Assistant Research Dynamic Energy

CV-Sunil Joshi-****

*

Sunil K. Joshi, DVM, PhD

*****************@*****.*** Phone # +1-757-***-**** INTRODUCTION:

A well-trained scholar (researcher and teacher) as Clinical & Cellular Immunologist focusing on innate and adaptive immunity, with major research interest in investigating the innate maturation of Dendritic Cells (DCs) followed by antigen cross-presentation via MHC-I to various T cell subsets. Further, curious to understand, how functional maturation of subtypes of DCs, influence the interaction with effector T cells, which determine the outcome of immune response in SELF (autoimmunity- T1D / MS) or NON-SELF (Cancers, Allografts, Infections- Malaria, Schistosoma & Toxoplasma, TB, Listeria, Tetanus & Anthrax, RSV, SARS-CoV2, HIV & Influenza) situations. As early-stage drug developer while working in the industry, I further extended my interest in the understanding of immune-dysregulation and immuno-pathogenesis of hereditary hemoglobinopathies (sickle cell / thalassemia) and hematological malignancies. I have a mindset of a "Global Citizen and Servant Leadership", I cultivate and reinforce high performance teams with strong work ethics to utilize innovative work methods through social interaction and team spirit to achieve performance-based recognition and excellence. I strive to create a positive and productive work environment by fostering diversity and the inclusion of minorities. I strongly believe that interpersonal, cross-cultural and multidisciplinary approaches can achieve ambitious goals. I manage my teams with compassion, emotional intelligence, and dynamic energy that inspires and empowers every person working around me. I am blessed with the intense passion to learn and to take on new challenges by applying strategic intelligence. AREAS OF EXPERTISE:

Multidisciplinary experience in Fundamental & Clinical Immunology applicable to Cancer, Infectious Diseases, Autoimmunity and Inflammation, Tissue Transplantation and In Silico Omics applications. Experience running Biomedical research programs & managing animal research, laboratory personnel and equipment. Proficient in immunological and molecular biology literature, techniques, and analysis. Subject matter specialist and highly experience in research design process, research integrity, Bioethics and regulatory policies of IACUC (PHS / National Centre for the Replacement, Refinement and Reduction of animals in research - NC3Rs / ARRIVE), CDC, FDA, AAALAC. Experience in administrating vivarium and ABSL-3, biological and radiation safety as University Veterinarian. Highly trained in Cellular immunology experiments / functional characterization of cellular immunogenicity, immune-monitoring and data analysis / interpretation in mouse and primate model (specific pathogen free Baboon), and humans (in vivo / ex vivo / in vitro functional characterization and data analysis). Histology & Imaging; Tissue culture & Cell line maintenance; Propagation and Biorepository. Molecular Cloning and Protein Expression & Purification. Human and Mouse T & B cells, Dendritic Cells, NKT cells Isolation, Purification and Functional Characterization. Many years of working experience in GLP settings in various labs. WORK EXPERIENCE:

I have more than 25 Years of robust research, teaching and administration experience in the Academia with overlapping 9 years of the Pharma Industry experience in drug development and pre-clinical research in mouse models, non-human primates and human subjects. Highly experience in working on bench in wet lab, presenting data in conferences and meetings, keynote speaker, writing research articles, editorials, reports & grants. I am well familiarized with regulatory components of Biomedical Research as per CDC / FDA / HHS. CV-Sunil Joshi-2022

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TOTAL PEER-REVIEWED PUBLICATIONS INCLUDING BOOK CHAPTERS: 69 SUCCESSFUL FEDERAL / PRIVATE GRANTS: Total 6 (~$ 8 million in funding) CITIZENSHIP: United States of America

PERSONAL STATEMENT:

I strive to create a positive and productive work environment by fostering diversity and the inclusion of minorities. I strongly believe that interpersonal, cross-cultural and multidisciplinary approaches can achieve ambitious goals. I manage my teams with compassion, emotional intelligence, and dynamic energy that inspires and empowers every person working around me. SUBJECT MATTER EXPERT:

Autoimmune Disorders and Allergies.

Neurodegenerative Disorders: Huntington's Disease, Alzheimer's Disease, Parkinson's Disease and Amyotrophic lateral sclerosis (ALS) Motor neuron disease.

Metabolic Syndromes: Obesity, Cardiovascular and Liver Diseases, Type 2 Diabetes, Diabetic Retinopathy, Peripheral Neuropathy

Kidney Dysfunctions: Chronic kidney disease (CKD), Amyloidosis, IgA Nephropathy, IgA Vasculitis, Diabetes insipidus, Lupus Nephritis and Glomerular Diseases

Immunodeficiencies and Infectious Diseases.

Immuno-oncology, Tumor Immunotherapies, CAR-T cells, Cellular Immunotherapy.

Transplantation Immunology and Immunosuppression, GVHD.

Rare and inherited Diseases: Sickle cell disease, Beta-Thalassemia, Immune aplastic anemia, Acquired hemophilia A (AHA), Acquired hemophilia B (AHB), Acute nonlymphocytic leukemia or Acute Myeloid Leukemia, Ichthyosis, Fanconi Anemia, AML, Addison’s Disease, hypogammaglobulinemia, Alkaptonuria, Bullous Pemphigoid, DiGeorge syndrome with athymia, Cystic Fibrosis, Hodgkin's Lymphoma, Guillain-Barré Syndrome, Cushing syndrome, Arginine: Glycine Amidino transferase Deficiency, Immunodeficiency with Ataxia Telangiectasia, Cerebral cavernous malformation (CCM), Louis-Bar Syndrome, Bechet’s disease, Autoimmune Hepatitis, Duchenne Muscular Dystrophy, Ataxia Cerebral Palsy, and Glucose-6-Phosphate Dehydrogenase Deficiency. MOST RECENT AFFILIATION & RESPONSIBILITIES:

Assistant Professor / Lab Director (03/2018 – 05/2020): Division of Hematology / Oncology & Bone Marrow Transplantation, Department of Pediatrics, University of Miami Miller School of Medicine, Miami, FL, USA.

Managing Lab of Transplantation Immunology – Allogeneic Bone Marrow Transplantation in humanized mouse model for hemoglobinopathies. Designing and developing protocols for cellular immunotherapy to alleviate the GVHD and B cell malignancy.

Conceptualizing, designing and developing cellular immunotherapy regimens involving iNKT, MDSc ex vivo expansion and in vivo transplantation with or without X-ray irradiation.

Providing hands-on training to MD/PhD students, postdoc and research scientists for in vitro and in vivo assays: Managing animal colony and designing construct for genetically engineered conditional knock-In PD-1 mouse model; Animal Disease models; Tissue and organ isolation; Immune cell isolation by AutoMACS and Cell Sorter; Immunophenotyping using multicolor flow cytometry and CyTOF / Hyperion; X-ray irradiation (Total Lymphocyte Irradiation-TLI and total bone marrow irradiation-TBI) & Bone marrow chimera generation; Apoptosis, CFSE-Cell proliferation assays both ex vivo and in vivo; Cytokine profiling and CV-Sunil Joshi-2022

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Inflammatory Markers using high-throughput assays; ELISA, ELISPOT, Immunoblot; mTOR Cell signaling and Innate immune response analysis; Data analysis and presentation.

Organizing Advanced Clinical Immunology Educational Course in Jaipur, India under FOCIS organization (March 26-30, 2019).

University services by serving on regulatory committees (IACUC, IBC, Radiation) as required.

Independently writing new proposals, manuscripts and protocols; presenting work in meetings and conferences.

EDUCATIONAL BACKGROUND:

Post-doctoral Fellow (PDF), Molecular Immunology / Gene Manipulation / Protein Transduction. Medical College of Georgia, Augusta University, Augusta, GA, USA

Doctor of Philosophy (PhD): Structural & Cellular Immunology, DBRU / Agra University

/ JALMA and International Center for Genetic Engineering & Biotechnology (ICGEB), New Delhi, India

Master of Veterinary Science (MVSc): Clinical Immunology / Virology Residency, Indian Veterinary Research Institute, Izzatnagar, India

Doctor of Veterinary Medicine (DVM): GBPUAT, Pantnagar, India

Bachelor of Science (BS): Biology & Chemistry, DBRU / Agra University, Agra, India CERTIFICATIONS / LICENSE:

Executive Education Certification (EEC): Management and Leadership Massachusetts Institute of Technology (MIT)-Sloan School of Management, Boston, MA, USA

Clinical Veterinary Practitioner (small animals), India:

• Uttar Pradesh State Veterinary Registration No. UPSVC / 354 / 92

• Indian Veterinary Practice Registration No. 224 / 05 / 27 / 98

Certified IACUC-Veterinarian & Animal Welfare Evaluator: Trained at Scientist Center for Animal Welfare “IACUC Advanced Workshop” at Cornell University, Ithaca, NY. PROFESSIONAL COMPETENCIES:

My technological expertise includes cellular, molecular, analytical and structural immunological techniques in both specialized technological platforms to perform proof-of-concept studies and translational innovative research by applying in silico, in situ, in vitro, ex vivo and in vivo methods. I have used in vitro tissue/cell culture methods and multiple animal models (Mouse

/ Primates / Bovines) to investigate the functions of immune system at the interface of cancer, pathogens, auto-immunity, rare genetic diseases, and allogeneic tissue transplantation. I rigorously maintain a current understanding of where the field of innate and adaptive immunology is headed through constantly reading current literature, attending seminars / webinars and conferences. More specifically, I am very well trained and have substantial bench experience in the following techniques along with supporting skills:

Cellular and Structural Immunology, Basic and translational concepts.

Routine biochemical techniques, molecular cloning, protein purification, structural and functional characterization for cGMP grade.

More than 7 years of experience in the industry-relevant recombinant protein process development for GMP clinical-phase products. Specifically, I have cloned, expressed, purified and functionally characterized: TB (Mycobacterium bovis) culture filtrate proteins, Plasmodium falciparum blood-stage parasite protein (MSP-1) / synthetic peptides, TAT- CV-Sunil Joshi-2022

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Cre Recombinase, Protective antigen (PA) and lethal factor (LF) proteins of anthrax

(Bacillus anthracis) by column chromatography (by means of AEX, CEX, affinity, SEC, HIC, HPLC, FPLC). I have used Forma scientific and Waters / Shimadzu HPLC system and Ultrafiltration of proteins by Tangential Flow Filtration (TFF).

Purified proteins characterized by Qualitative assays including SDS-PAGE and Western Blot; Quantitative assays using UV-Visual spectrophotometer / Mass-spectrometry; functional assays using cell lines, flow cytometry, animal models.

Knowledge and use of statistical design of experiments (DoE).

Animal models to study immune system, cancer and infections models.

Deep knowledge of Innate and Adaptive Immunity fundamentals and new developments.

Isolation, purification and phenotypic / functional characterization of various immune cells

(Macrophages, Neutrophils, T / B Lymphocytes, Dendritic cells, NK, NKT, gd-T cells, MDSc, MSC) from blood and various tissues sources in both animals and humans.

Mammalian cell / tissue culture, ex vivo / in vitro assays, T cell cloning and in vitro NKT cell expansion (both in mouse and human).

Immune monitoring through the use of multi-color Flow Cytometry and intra-cellular cytokine staining; Flow data analysis; ELISA; Western Blot; ELISPOT; MHC-Tetramer / CD1d-Tetramer staining; multiplex cytokine analysis; ex vivo / in vivo T cell proliferation and cytotoxicity.

Bone Marrow Chimera and allogeneic bone marrow transplantation in humanized mice. Antigen cross presentation assays and in vivo adoptive transfer of ex vivo-matured antigen-loaded dendritic cells and in vivo cell-tracking.

Basic molecular biology techniques: PCR, RT-PCR, mRNA, molecular cloning, protein expression, folding and purification.

Light Microscopy, Live cell imaging, confocal and fluorescent microscopy and data analysis.

Deep technical understanding of ‘omics and their application in target discovery through experience of using IMMPORT / Immunology OMICS.

Bioinformatic analysis of genome / proteins sequences; Data mining; Data management; Data analysis; Statistical Analysis; MS Office-Windows; Adobe Photoshop; GraphPad Prizm; FCS Express and Flow Jo.

Proven track record in successfully leading or co-leading large complex public health research projects, including multicenter coordinating centers.

Successfully obtained funding from NIH, Bill & Melinda Gates Foundation, Faculty startup grant and grants from pharmaceutical company.

Demonstrated track record of leadership in developing and sustaining a climate that fosters growth in research funding, staff capabilities, and scientific excellence and reputation.

Experienced in leading multi-disciplinary teams including senior, mid-level and junior researchers, statisticians, programmers and research coordinators/project managers.

Proven record of scientific stature in presentation and publication of study results in peer- reviewed journals and other venues.

Proven record in successful grant writing and obtaining extramural research funds.

Excellent skills in communication, negotiation, compromise, and building consensus within and between groups.

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EMPLOYMENT RECORD:

Assistant Professor (Cellular Immunotherapy Lab Director): Division of Hematology / Oncology & Bone Marrow Transplantation, Department of Pediatrics / Microbiology & Immunology, University of Miami Miller School of Medicine, Miami, FL (Mentor: Julio Barredo, MD Pediatrics). 03/2018 – 05/2020.

Assistant Professor (tenure-track): Frank Reidy Research Center for Bioelectrics and School of Medical Diagnostic and Translational Sciences, Old Dominion University, Norfolk, VA. 03/2013 – 06/2017.

Adjunct Teaching Faculty: Department of Microbiology & Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA. (Dianne C. Daniel, PhD, Associate Professor and Biotechnology Program Director). 03/2013 – 06/2017.

Senior Scientist, Early Drug Development: EpimedX / SCCF, Oklahoma City, OK. Pharmacological testing of EDX-17 pipe-line drug to increase the induction of fetal hemoglobin in Sickle cell disease BERK-mouse model. 01/2010 – 12/2019.

Assistant Professor of Research: DTRA Research Scholar, Department of Microbiology and Immunology, Primate Immunologist, Comparative Medicine Oklahoma University Health Science Center (OUHSC), Oklahoma City, OK (Mentor: Mark L. Lang, Ph.D.). 08/2008 – 03/2013.

University Veterinarian / ABSL-3 Vivarium In-charge: Supervising ABSL-3 Vivarium, IACUC approval, Training for Mouse procedures, Infection monitoring and managing supporting staff and Veterinary technicians. Augusta University, Augusta, GA. 05/2005 – 04/2007

Postdoctoral Research Associate: Molecular Immunology Program at Institute of Molecular Medicine and Genetics; Assistant Research Scientist / Immunologist, ABSL-3 Program In-charge, and attending Clinical Veterinarian, Laboratory Animal Resources, Medical college of Georgia, Augusta, GA (Mentor: Pandelakis A. Koni, Ph.D). 10/1999 – 04/2005.

Visiting Research Fellow: Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, Hôpital de la Pitié-Salpêtrière, Paris, France. 08/1998 – 02/1999.

Research Fellow and In-charge vivarium: Malaria Research group at International Center for Genetic engineering and Biotechnology (United Nations), New Delhi, India. 01/1995 – 07/1998.

COMPLETED FUNDED PROJECTS:

NIH-P40: 2 P40 OD 010988-16 (Parent grant PI: White, OUHSC, Oklahoma City, OK) Project Title: “Baboon Research Resources Program (2013-2018)”; Title (Sub- Project 4):

“Immunity to Plasmodium knowlesi Malaria in Baboons” (PI: JOSHI).

ODU-Center of Bioelectrics Startup Funds (PI: JOSHI 2013 - 2016): As a tenure-track faculty, I have been provided all equipment (major & small) and common reagent supplies, research staff and discretionary funds to establish cellular immunology lab and develop new research areas relevant to my experience and expertise. The funds are being used to purchase experimental animals, pay core and vivarium expanses, undergraduate / graduate education & training, continuing education & learning new technologies, publication fee and purchasing specific biological / chemical / molecular reagents. The major part of this fund is also being used in proteomics and mass-spectroscopic analysis of proteins expressed before and after pulsed-electrical field application using both mouse and human purified Dendritic cells.

EpimedX / SCCF Pilot grant: PI: JOSHI 04/30/2015-12/31/2015 Title: “Pathophysiology of Sickle cell disease in transgenic mouse models”.

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NIH-U19: AI062629 (PI: Coggeshall, OMRF and Lang, OUHSC) U19 Parent Grant. Title: Molecular and Immunologic Analysis of the Pathobiology of Anthrax Title: “Anthrax toxin- induced anergy in primary human NKT cells” (sub-project: Joshi 2011 - 2012).

Bill & Melinda Gates Foundation: Grand Challenge Exploration (GCE Round 4) OPP1015489 Title: “Development of Safe, Cost-effective, and Functional Strategy for Immune Intervention” (2010 – 2012).

NIH / NCRR-G20: 1G20RR024019-01 (Facility Improvement Grant) Title: “Developing and Improving Institutional Animal Resources” (2006).

PEER REVIEWED PUBLICATIONS: Total 69

Original Research Articles: Total 40

1. Hemanth K. Manikyam, Sunil K. Joshi, Spandana Vakadi and Sandeep Balavant Patil.2021

"Anticancer activity of terpenoid saponin extract of Psidium guajava on MCF-7 cancer cell line using DAPI and MTT assays." African Journal of Pharmacy and Pharmacology 15, no. 12 (2021): 206-211. 2. Hemanth K. Manikyam*, Sunil K. Joshi, Malinidevi. M, Afeefa Noor. 2020. Ayurveda and Siddha systems polyherbal formulations to treat COVID-19 caused by SARS-CoV- 2 and brief insight on application of Molecular Docking and SWISS Target prediction tools to study efficacy of active molecules. International Journal of Phytomedicine [S.l.], v. 12, n. 2, p. 35-41, jun. 2020. DOI: 10.5138/09750185.2409.

3. Hemanth Kumar Manikyam* and Sunil K Joshi*. 2020. Dammarane and Ergostane derivatives as prophylactic agents against SARS-CoV-2 host cell entry Inhibitors. Journal of Pharmacognosy and Phytochemistry. 2020; 9(3): 1211-1216.

4. Hemanth K Manikyam*, Sunil K Joshi*. 2020. Computational methods to develop potential neutralizing antibody Fab region against SARS-CoV-2 as therapeutic and diagnostic tool. BioRxiv 2020.05.02.071506. DOI:10.1101/2020.05.02.071506.

5. Hemanth K. Manikyam*, Sunil K. Joshi. 2020 Computational studies on Gene Ontology for Molecular functions, Cellular component and biological process of SARS-CoV-2 targeted proteins. PREPRINT AT RESEARCH SQUARE 2020.05.04. DOI:10.21203/rs.3.rs-26263/v1. 6. Manikyam HK* and Joshi SK*. 2020. Whole Genome Analysis and Targeted Drug Discovery Using Computational Methods and High Throughput Screening Tools for Emerged Novel Coronavirus (2019- nCoV). J Pharm Drug Res, 3(2): 341-361. NIHMS ID:1582187. 7. Hemanth Kumar Manikyam* and Sunil K Joshi. 2020. “Nicotinamide, Folic Acid and Derivatives as Potent Inhibitors of Inflammatory Factors against Novel Corona Virus Infection". Acta Scientific Pharmaceutical Sciences 4.5 (2020): 27.

8. Khandia R, Pattnaik B, Rajukumar K, Pateriya A, Bhatia S, Murugkar H, Prakash A, Pradhan HK, Dhama K, Munjal AK and Joshi SK*. 2017. Anti-proliferative role of recombinant lethal toxin of Bacillus anthracis on primary mammary ductal carcinoma cells revealing its therapeutic potential. Oncotarget. May 30;8(22):358**-*****. PMID:28415766.

9. T. Scott Devera, Dawn Kennedy, Sunil K. Joshi, Jimmy D. Ballard and Mark L. Lang. Immunization of mice with anthrax Protective Antigen limits cardiotoxicity but not hepatotoxicity following Lethal Toxin challenge. Toxins 2015, 7, 2371-2384.

10. Julianna Pryor, Brittany Collins and Sunil K. Joshi*. A transgenic mouse model correlates anomalous immune dynamic in human sickle cell disease (HUM1P.256). J Immunol. May 1, 2015, 194 (1 Supplement) 52.5.

11. Sunil K. Joshi*. High-intensity ultra-short electric pulse applications in modulating innate immunity: views on cutting-edge research. The Biomedical Scientist 2015 March; 59 (3): 146-7. 12. Robert Broyles, Sunil K. Joshi, Carol Curtis, Austin Roth, Patrick Floyd, Marie Trudel, Visar Belegu and Robert Floyd. EdX-17: a novel, safe and efficacious treatment for Sickle Cell Disease. Blood 2014; 124:1357 (21).

13. Sunil K. Joshi*. Enhanced functional maturation of dendritic cells by exponential wave electric pulse application. J. Clin. Cell Immunol. 2014, 5.5.

14. Robert Broyles*, Austin Roth, Robert Floyd, Visar Belegu, Emily Curry, Carol Curtis, Marie Trudel, Paolo Santambrogio, Sonia Levi, Paolo Arosio, and Sunil K. Joshi* (2013). Nuclear Ferritin: From Discovery CV-Sunil Joshi-2022

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to Promising Treatments to Stop Sickle Cell Disease and Malaria: Visits from the Three Princes of Serendip and Recent Progress. American Journal of Hematology Volume 88, Issue 5, pages E5– E243, May 2013.

15. Robert Broyles*, Austin Roth, Carol Curtis, Robert Floyd, and Sunil K. Joshi* (2013) Edx−17, A Safe Phytohormone that Promises a Phenotypic Cure for Sickle Cell Disease and a Blocking Treatment for Malaria: In Vivo Proof of Principle in Animals. American Journal of Hematology Volume 88, Issue 5, pages E5–E243, May 2013.

16. Sunil K. Joshi*, David Carey, Maria Chavez-Suarez, Mark Lang, Robert Broyles, and Gary White. Immune dysregulation in a major hematologic disease J Immunol. 2013 190:43.45. 17. Shah HB, Joshi SK, Rampuria P, Devera TS, Lang GA, Stohl W, Lang ML. BAFF- and APRIL- dependent maintenance of antibody titers after immunization with T-dependent antigen and CD1d- binding ligand. J Immunol. (2013) Vol 191(3):1154-1163. 18. Joshi SK*, Lang GA, Devera TS, Johnson AM, Kovats S, Lang ML. (2012) Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation. J Leukoc Biol. May;91(5):783-90.

19. Mark Lang, Hemangi Shah, Sunil K. Joshi, Susan Kovats, Gillian Lang, and T. Devera. CD1d expression by dendritic cells and CD40L or ICOS expression by NKT cells is dispensable for NKT- enhanced humoral immunity. J. Immunol. 2011 186:61.16. 20. R. H. Broyles, Sunil K. Joshi, V. Belegu, A. C. Roth, E. J. Curry, R. A. Floyd, S. Levi, P. Santambrogio, P. Aroslo and M. Trudel (2011). In vivo testing in mouse models using ferritin heavy chain and abscisic acid as potential therapies for malaria, sickle cell disease and beta thalassemias. American Journal of Hematology Volume 86, Issue 9;17 AUG 2011.

21. R. H. Broyles, Visar Belegu, Austin C. Roth, Emily J. Curry, Robert A. Floyd, Sonia Levi, Paolo Arosio, Paolo Santambrogio, Marie Trudel and Sunil K. Joshi* (2011). Ferritin-H and a Phytotherapeutic, Alone or Combined, Reprogram RBC Precursor Cells from SCD Patients to Produce Levels of Fetal Hemoglobin That Constitute a Phenotypic Cure for Sickle Cell as Well as Providing Resistance to Malaria and a Probable Treatment for Beta-Thalassemia. Blood 2011 118:903. 22. H. B. Shah, Sunil K. Joshi and Mark L. Lang (2011). CD40L-null NKT cells provide B cell help for specific antibody responses. Vaccine 29, 9132–9136. 23. T. Scott Devera, Sunil K. Joshi, Lindsay M. Aye, Gillian A. Lang, Jimmy D. Ballard and Mark L. Lang

(2011). Regulation of anthrax toxin-specific antibody titers by Natural Killer T cell-derived IL-. PLoS ONE 6(8): e23817.

24. G. A. Lang, Johnson A. M., Devera T. S., Sunil K. Joshi and M. L. Lang (2011). Reduction of CD1d expression in vivo minimally affects NKT-enhanced antibody production but boosts B-cell memory. International Immunol. (4):251-260.

25. Elizabeth Charles, Sunil K. Joshi*, John D. Ash, Barbara Fox, A. Darise Farris, David J. Bzik, Mark L. Lang, and Ira J. Blader. CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1 (2010). Infection and Immunity Aug;78(8):3484-3492. *Equal contribution.

26. T.S. Devera, L.M. Aye, G.A. Lang, Sunil K. Joshi, J.D. Ballard and M.L. Lang (2010) CD1d-dependent B-cell help by NK-like T cells leads to enhanced and sustained production of Bacillus anthracis lethal toxin-neutralizing antibodies. Infection and Immunity (4):1610-1617. 27. Sunil K. Joshi*, Gillian Lang, T. Scott Devera, Lindsay M Aye, Hemangi B Shah, Jimmy Ballard, and Mark Lang. Bacillus anthracis lethal toxin abrogates TCR- induced cytokine production by CD1d- restricted Natural killer like - T cells J Immunol. 2009 182:134.15 28. Sunil K. Joshi*, G. A. Lang, J. Larabee, L. M. Aye, T. S. Devera, H.B. Shah, J. D. Ballard, and M. L. Lang. Bacillus anthracis lethal toxin disrupts TCR signaling in CD1d- restricted NKT cells leading to functional anergy. (2009) PLoS Pathogens 5(9): 1-10. 29. Degui Geng, Sunil K. Joshi, and J. X. She. GCSF receptor regulates antigen cross- presentation and expression of cytokines and costimulatory molecules in dendritic cells. (2007) Molecular Immunology Jan; 44(4): 521-529.

30. M. Shimoda, F. Mmanywa, Sunil K. Joshi, T. Li, K. Miyake, J. Pihkala, J. A. Abbas, and P. A. Koni. Conditional ablation of MHC-II suggests an indirect role for MHC-II in regulatory CD4 T cell maintenance.

(2006), Journal of Immunology, June 1; 176 (11): 6503-6511. CV-Sunil Joshi-2022

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31. Qing-Sheng Mi, Zhong-Bin Deng, Sunil K Joshi, Zai-Zhao Wang, Li Zhou, Sarah Eckenrode, Ratanmani Joshi, Dalam Ly, Bing Yi, Terry L Delovitch & Jin-Xiong She. The autoimmune regulator (Aire) controls iNKT cell development and maturation. Nat Med. 12, 624–626 (2006). 32. J. Pablo Abonia, K. Frank Austen, Barrett J. Rollins, Sunil K. Joshi, Richard A. Flavell, William. A. Kuziel, Pandelakis A. Koni, and Michael F. Gurish. Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2. (2005) Blood, June 1; 105 (11) 4308-4313.

33. Sunil K. Joshi, K. Hashimoto, and Pandelakis A. Koni. Induced DNA Recombination by Cre Recombinase Protein Transduction. (2002) Genesis, May; 33 (1): 48-54. 34. K. Hashimoto, Sunil K. Joshi, P. A. Koni. A conditional null allele of the major histocompatibility IA-beta chain gene. (2002) Genesis, Feb; 32 (2): 152-3.

35. P. A. Koni, Sunil K. Joshi, U. A. Temann, D. Olson, L. Burkly, R. A. Flavell. Conditional vascular cell adhesion molecule 1 deletion in mice: impaired lymphocyte migration to bone marrow. (2001), Journal of Experimental Medicine. 19; 193 (6): 741-54.

36. Sunil K. Joshi, P. Suresh and V. S. Chauhan. Flexibility in TCR recognition: Degenerate specificity at the T cell level in the recognition of promiscuous TH epitopes exhibiting no primary sequence homology.

(2001), Journal of Immunology, June 1;166 (11): 6693-703. 37. Sunil K. Joshi, A. Bharadwaj, S. Chatterjee, and V. S. Chauhan. Analysis of Immune Responses against T and B Cell Epitopes from Plasmodium falciparum Liver Stage Antigen-1 (PfLSA-1) in Rodent Malaria Models and malaria-exposed Human Subjects in India. (2000), Infection and Immunity, Vol. 68

(1): 141-150.

38. A.V. Pandey, Sunil K. Joshi, B. L. Tekwani and V. S. Chauhan. A colorimetric assay for heme in biological samples using 96 wells plate. (1999), Analytical Biochemistry, Vol. 268, p 159-161. 39. Bharadwaj, P. Sharma, Sunil K. Joshi, B. Singh and V. S. Chauhan. Induction of protective immune responses by immunization with linear multiepitope peptides based on conserved sequences from Plasmodium falciparum antigens. (1998), Infection and Immunity, Vol. 66, No.7, p 3232-3241. 40. Bharadwaj, P. Sharma, V. N. Sailaja, Sunil K. Joshi and V. S. Chauhan. Immunogenicity of synthetic peptides containing multiple epitopes from malaria antigens. (1997), Annals of Tropical Medicine and Parasitology, Vol 91, No. 1, p S19-S20.

Review Articles: Total 15

1. Gowthaman V, Kumar S, Koul M, Dave U, Murthy TRGK, M P, Tiwari R, Karthik K, Dhama K, Michalak I, Joshi SK*. Infectious Laryngotracheitis: Etiology, Epidemiology, Pathobiology, and Advances in Diagnosis and Control - A Comprehensive Review. The Veterinary quarterly. 2020 Apr 21:1-30. PMID: 32315579.

2. Dhama K, Latheef SK, Dadar M, Samad HA, Munjal A, Khandia R, Karthik K, Tiwari R, Yatoo MI, Bhatt P, Chakraborty S, Singh KP, Iqbal HMN, Chaicumpa W and Joshi SK. 2019. Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values. Frontiers in Molecular Biosciences. 6:91. doi: 10.3389/fmolb.2019.00091.

3. Khandia R., Dadar M., Munjal A., Dhama K., Karthik K., Tiwari R., Yatoo MI., Iqbal HMN., Singh KP., Joshi SK*, Chaicumpa WA. 2019. Comprehensive Review of Autophagy and Its Various Roles in Infectious, Non-Infectious, and Lifestyle Diseases: Current Knowledge and Prospects for Disease Prevention, Novel Drug Design, and Therapy. Cells. Jul 3;8(7). pii: E674. 4. Anouk A. J. Hamers, Sunil K. Joshi, Asha B. Pillai. 2019. Innate Immune Determinants of Graft-Versus- Host Disease and Bidirectional Immune Tolerance in Allogeneic Transplantation. OBM Transplantation. doi:10.21926/obm.transplant-1901044.

5. Dhama K, Karthik K, Khandia R, Munjal A, Tiwari R, Rana R, Khurana SK, Sana Ullah, Khan RU, Alagawany M, Farag MR, Dadar M, and Joshi SK.2018. Medicinal and Therapeutic Potential of Herbs and Plant Metabolites / Extracts Countering Viral Pathogens - Current Knowledge and Future Prospects. Curr Drug Metab.19(3):236-263. PubMed PMID:29380697. 6. Munjal A, Khandia R, Dhama K, Sachan S, Karthik K, Tiwari R, Malik YS, Kumar D, Singh RK, Iqbal HMN, Joshi SK. 2017. Advances in Developing Therapies to Combat Zika Virus: Current Knowledge and Future Perspectives. Frontiers of Microbiology. Aug 3;8:1469. PMID:28824594 7. Singh RK, Dhama K, Malik YS, Ramakrishnan MA, Karthik K,

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