Research Fellow Public Health

Gbemi Madarikan, MS, MPH, MB(ASCP)CM Page * of *

Gbemi Madarikan

Derby, CT 06418 848-***-****

**********@*****.*** LinkedIn: http://linkedin.com/in/gbemi-madarikan-84381a103 A recent graduate of Master of Public Health with a solid background in science and transferable skills including laboratory skills. My interests within the Public Health field include epidemiology, infectious diseases prevention, outbreak response, and community health education. Possess excellent interpersonal skills with clear communication in both written and verbal format, and a strong ability to work both in a team environment and individually with a positive and diligent attitude. EXPERIENCE

Integrated Oncology, Shelton, CT Dec 2021-Present

Senior Molecular Technologist

• Responsible for advanced technical duties including QC, calibrations, and troubleshooting of technical problems

• Assist with the management of the laboratory through operations maintenance, supervision of personnel, quality assurance and proficiency testing

• Assist in quality management including personnel training, testing, and quality review

• Ensure inspection readiness and tracer activities for appropriate regulatory and accreditation agencies

• Maintain laboratory inventory of reagents including ordering and validations Integrated Oncology, Shelton, CT Feb 2018- Dec 2021 Molecular Technologist

• Contributed to the validation process of the standard operating procedures preceding inspection by the College of American Pathologists and other state agencies and use in clinical diagnosis on-site.

• Performed routine manual and automated molecular tests including oncology-based PCR (RT-PCR, real-time PCR using the 7900HT-Fast Real time PCR systems and end-point PCR amplification using the GeneAmp 9700 PCR system and Tetrad 2 PCR machine), nucleic acid extractions and quantitation (using the QIAcube and Tecan Infinite 200, respectively), capillary electrophoresis using the 3130xl genetic analyzer and fragment analysis using the Gene Mapper software as outlined in the standard operating procedure (SOPs) for advanced cancer diagnosis.

• Troubleshoots problems when nonconformities occur by using critical reasoning to analyze each situation and proffer solutions within the established turn-around times for clinical testing.

• Ensure quality in testing is maintained within the laboratory through reagent validations, and equipment maintenance as a quality ambassador

Yale University, New Haven, CT Jul 2017-Nov 2017

Postgrad Research Fellow

Project: Roles of Hsp90 in regulating epithelial mesenchymal transition (EMT) in ovarian cancer

• Produced data supporting EMT-reversal hypothesis via intracellular Hsp90 inhibition and promoter activity overexpression factors by luciferase assay.

• Optimized RNA extraction and cDNA synthesis conditions (critical genes in 2 carcinoma models by real-time PCR) and treatment conditions (cytotoxicity studies: dose and duration of 17-AAG (Hsp90 inhibitor)).

• Reproduced experiments in normal, embryonic cells as quality control measures for the experimental cell lines.

• Optimized the cell density and incubation time conditions to promote high plasmid transfection efficiency in culturing the ovarian cancer cell lines.

• Utilized and compared multiple western blot detection techniques to ensure high result accuracy of Hsp90 protein expression.

Gbemi Madarikan, MS, MPH, MB(ASCP)CM Page 2 of 3

• The findings from this project were published in a scientific research journal (Chong et al., 2019). Yale University, New Haven, CT Jun 2016-Aug 2016

Postgrad Research Fellow

Project: CRISPR/Cas9 system knockout for pooled lentiviral library production in ovarian cancer cell lines

• Performed resistance screens on 5 target primary cell lines following successful nuclear viral infection by HEK293t mediated chemical and electroporation-based transfection techniques.

• Conducted validation experiments to confirm CRISPR-mediated gene knockdown and expression levels of Cas9-directed RNAi knockdown using quantitative Real-time PCR (qRT-PCR) in target genes.

• Optimization of pooled screening using an enhanced GFP-dCas9 fusion protein as a reporter system to indicate successful transfection.

Redeemer’s University, Ede, Nigeria Oct 2012-Jul 2013 Undergrad Research Student

Project: Effect of virgin coconut oil on Trimethoprim-Sulfamethoxazole toxicity in Wistar rats

• Designed dose-dependent, combination studies to determine the minimum toxicity levels that affect normal organ biochemistry.

• Performed in-depth toxicity studies on cell lysates from 20 euthanized rats specifically investigating the enzyme kinetics of liver, lungs, and testes samples using biochemical assays.

• Findings from this study indicating that virgin coconut oil reduces hepatotoxicity in Wistar rats were published in a scientific journal (Otuechere et al., 2014).

The Premier Specialists’ Medical Center, Lagos, Nigeria Feb 2012-Aug 2012 Medical Laboratory Intern

• Performed consistent chemical urinalysis and prepared specimen for microscopic examination contributing to patient diagnoses, care, and recovery processes

• Prepared various types of laboratory specimens prior to laboratory testing and result analysis; implementing Quality assurance steps to minimize errors prior to physicians’ review LABORATORY SKILLS

Real-time PCR, PCR, capillary electrophoresis, aseptic techniques, mammalian, bacterial and viral cell culture, bacterial transformation, cell fractionation, 3D cell culture, immunoprecipitation, protein production and purification, ELISA, western blot analysis, gel electrophoresis, gel extraction, nucleic acid extraction and purification, liposome-mediated plasmid transfection, CRISPR-mediated lentivirus transduction, luciferase assay caspase 3/7 assay.

STATISTICAL & DATA ANALYSIS SYSTEMS

GeneMapper, R software, SPSS Statistical Software, Microsoft Office Proficient. EDUCATION

• University of Bridgeport, Bridgeport, CT May 2021-June 2022 Master of Public Health, with Concentration in Community Health

• University of New Haven, New Haven, CT Aug 2015-Dec 2017 Master of Science, Cellular and Molecular Biology

• Redeemer’s University, Ede, Nigeria Sep 2009-Jul 2013 Bachelor of Science (Hons.), Biochemistry

CERTIFICATIONS

• American Society for Clinical Pathology, MB(ASCP) July 2021 Technologist in Molecular Biology, Cert. # 5991

Gbemi Madarikan, MS, MPH, MB(ASCP)CM Page 3 of 3

PUBLICATIONS

• Chong KY, Kang M, Garofalo F, Ueno D, Liang H, Cady S, Madarikan O, Pitruzzello N, Tsai CH, Hartwich TMP, Shuch BM, Yang-Hartwich Y. HSP90 Inhibitor Suppresses Twist1 Transcription. Molecular Pharmacology August 2019, 96 (2) 168-179; DOI: https://doi.org/10.1124/mol.119.116137.

• Otuechere CA, Madarikan G, Simisola T, Bankole O, Osho A. Virgin coconut oil protects against liver damage in albino rats challenged with the anti-folate combination, trimethoprim-sulfamethoxazole. J Basic Clin Physiol Pharmacol. 2014 May 1; 25(2):249-53. doi: 10.1515/jbcpp-2013-0059. REFERENCES

• Available upon request.

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