Medical Assistant Scientist

CURRICULUM VITAE

Ali Zendedel Haghighi

Home Address: **** ********* *****

San Jose, CA 95120

Phone: 510-***-**** (cell)

408-***-**** (home)

E-mail: **********@*****.***

Employment:

LamdaGen Corporation

Menlo Park, CA

Scientist for Bioassay development

2021-present

Mega Pre College-prep

Cupertino, Ca

Science teacher ( Biology, Chemistry and Physics)

Mentor for the research projects

2019-2021

Translational Bioscience

Sunnyvale, Ca

Cell Culture Lab Manager

2018-2019

Harker High School

San Jose, Ca

Instructor for Chemistry and Biology

2016-2018

National Hispanic University

San Jose, Ca

Instructor for Chemistry and Biology

2012- 2016

Eeonyx Corporation

Pinole, Ca

Consulting Scientist on the conductive polymers’ projects 2013-2014

Chitotech Corporation

Consulting Scientist on the Wound healing products 2011-2012

American Century University

San Jose, CA

Setting up the Biotechnology program, as well as the instructor for the pro- gram

2010- 2012

Applied Chemicals

Sunnyvale, CA

Scientist for Biofuel projects

2009-2010

LamdaGen Corporation

Menlo Park, CA

Scientist for Bioassay development

2008-2010

2012-2013

Polygenetics, Inc.

Mountain View, CA

Senior Biochemist

2005-2009

GeneEd

San Francisco, CA

Editor for Biotech courses

2007-2008

San Francisco State University

San Francisco, CA

Instructor for the Clinical Trial program

March 2006- May 2006

CoolSkin Corp.

Los Angles, CA

Consultant for skin rejuvenation projects

2005- 2008

NovaCal Pharmaceuticals, Inc.

Emeryville, CA

Senior Scientist

2004- 2005

Bryman College

Hayward, CA

Science and Clinical Instructor

Surgical Technology

2001-2004

Genidis

San Jose, CA

Consultant for Bioinformatic Projects

2003-2004

Laserscope, Inc.

San Jose, CA

Consultant for the projects on the skin rejuvenation 2001- 2003

Sunrise Technologies, Inc.

Fremont, CA

Senior Scientist and Clinical Coordinator

1999-2001

MAST Immunosystems, Inc.

Mountain View, CA

Senior Scientist

1994-1999

Centaur Pharmaceuticals, Inc.

Sunnyvale, CA

Research Scientist

1993- 1994

The Cleveland Clinic Foundation

Cleveland, Ohio

Research Fellow

1990-1993

Education:

Ph.D. in Biochemistry

Cleveland State University, 1991

M.S. in Biochemistry

Kent State University, 1986

B.A. in Chemistry

Kent State University, 1982

Certification: American Heart Association, BLS Instructor Teaching Certification for the Surgical Technology Teaching Certification for the Medical Assistant

Renewable and Green Technology Projects:

Copper-plated TiO2, exposed to UV light, as an alternative catalyst to Pt in Fuel cells (Mega Prep)

Removal of water contaminants (chemical and Biological), using TiO2 and UV light ( Applied Chemicals)

Use of nanoparticles as catalyst for the production of Biodiesel fuel (Bio Dico)

Development of a process to convert polysaccharide contents of Biomass to mono-saccharides, at room temperature and 1 atmosphere pressure for the use in Biofuel production ( Applied Chemicals)

Experience (Biotech and teaching):

Senior scientist responsible for development of diagnostic assays using nano-particle surfaces and LSPR (Lamdagen Corporation) Instructor for AP and SAT Chemistry, Biology and Physics, as well as con- ducting research and supervising students on research projects related to fuel cells, diagnostics and removal of CO2 from atmosphere (Mega Pre College- Prep)

Manager of the cell culture lab, testing the effects of anti-cancer drugs on various cancer cell line (Translational Bioscience) Instructor for the High school Chemistry classes (Harker High School) Instructor for the first year college chemistry and biology courses (National Hispanic University)

Initiating and supervising the projects on the wound healing products, based on the silver-nano particles’ anti-septic properties (Chitotech Corporation) Setting up the Biotechnology program and functioning as the main instructor for the program (American Century University)

Assay development on biosensors, for the detection of biomolecules as well as drugs (LamdaGen Corporation)

Development of an ELISA-based diagnostic assay system, using Cavilink- TM polymers, as well as their application in drug delivery systems (Polyge- netics, Inc.).

Development of in vitro models and studies to test the effect of the Nova- cal’s products on wound healing, enhancement of collagen synthesis, as well as their ability to effectively destroy biofilm, generated by infecting bacteria. Also, studies on the selection of possible drug vehicle for the Novacal’s products. (Novacal Pharmaceuticals)

Instructor for the basic sciences (i.e., Anatomy, Physiology, Pharmacology and Microbiology) in a Surgical Technology training program. Also, clinical site instructor for the students receiving training at the area hospitals. (Bry- man College).

Study on the effect of Proteases and Reactive Oxygen Species (ROS) on the lens proteins and subsequent Cataract formation. (UCSF) Study on the role of oxidative damage and UV light in the development of Cataract and Presbyopia and, development of possible methods, to inhibit as well as reverse Cataract and Presbyopia. (Sunrise Technologies) Study on the mechanism of heat transfer in the cornea after laser treatment and development of a method to treat Myopia, using laser thermal kerato- plasty (LTK). Development of biological models to study the long-term ef- fect of laser treatment on the cornea and, development of a biochemical pro- cedure to reduce or inhibit the regression associated with the laser refractive surgery. Designing, implementing and coordinating clinical studies on the use of laser for the treatment of Myopia. Also, designing clinical studies on the biochemical treatment of the laser-treated hyperopia individuals. (Sunrise Technologies)

Development of a novel and simple procedure for the detection of immune complexes in serum, formed as the result of excessive food allergens.

(MAST Immunosystems)

Purification and characterization of a new allergenic protein in Mite farinae, using different protein purification methods such as: gel filtration chromatography, SDS-PAGE (both analytical as well as preparative), elec- troelution of protein molecules, Western blotting, ELISA and different pro- tein quantitation methods. (MAST Immunosystems)

Purification and characterization of proteins causing allergic reactions and coupling these proteins to a solid matrix to be used in an allergy-testing de- vice. Also, development of assays (chemical, biochemical and ELISA.) and writing protocols, SOPs, MBRs, QAP and QTS documents for these assays and protein purification procedures to be used by the QA and manufacturing departments. (MAST Immunosystems)

Study on the inhibitory effect of beta-amyloid peptide and methionine sul- foxide on glutamine synthetase and creatine phosphokinase, by the use of enzyme assays and free radical scavengers. (Centaur Pharmaceuticals) Setting up the Biochemistry laboratory in a startup company. Evaluating and developing biochemical assays, and procedures such as: assays for gluta- mine synthetase, creatine phosphokinase and lipoxygenase, protein and lipid oxidation assays and brain tissue preparation protocols and writing SOPs to screen compounds as therapeutic agents for neurodegenerative diseases.

(Centaur Pharmaceuticals)

Purification and characterization of a human immunosuppressive lym- phokine produced and released by a human thymus hybridoma, utilizing the techniques of cell culturing, antibody isolation and preparation, im- munoaffinity column preparation, enzyme immunoassay (EIA), antigen pu- rification and preparation, SDS-PAGE (both analytical and preparative), western blotting, bioassays for lymphokine activity measurements, protein purification and PCR. ( The Cleveland Clinic Foundation) Study of the mechanism of the low density lipoprotein oxidation by the acti- vated monocytes as well as the oxidation of pure lipids mediated by the free radicals, utilizing the techniques of cell culturing, TLC, lipid isolation and purification, lipid assays, assays for the detection and measurements of oxi- dized lipids and assays for the detection and measurements of different free radicals. (The Cleveland Clinic Foundation)

Study of enzymes released from PMNs due to the external stimulation, using the enzyme activity assays for various enzymes, protein assay, SDS-PAGE, the Superoxide Dismutase (SOD) enzyme kinetic studies, and development of a methodology for SOD activity measurements. Study of the effect of Phenobarbital treatment on the serum lipoprotein, Thromboxane A2 and Prostacyclin, using radioisotope labeling, PAGE and SDS-PAGE, protein purification as well as protein and cholesterol assays. Lipoprotein classification and their molecular weight distribution, by utiliz- ing the gradient PAGE and analytical ultracentrifugation. Development of methodology for purification of Herpes Simplex virus by use of skin Fibroblast cell culture, density gradient ultracentrifugation, pro- tein assay and SDS-PAGE.

Study of the changes in liver plasma membrane composition in the choles- terol-fed rats, using the preparative density gradient ultracentrifugation and phosphate and protein assays.

Study of a drug and oligonucleotide interaction, using virus infected skin fi- broblast cells and interferons.

Study of the methyl mercury toxicity in Bluegills using the protein and cho- lesterol assays and SDS-PAGE methods.

Publications:

1) Ali Zendedel Haghighi, LamDagen’s meta-catalytic surfaces, first case study: Role of the surfaces in the catalytic decomposition of hydrogen per- oxide, (2009), White paper, written as a part of the work in LamdaGen copr- poration.

2) Ali Zendedel Haghighi, James R. Benson and Nai-Hong Li, Use of highly porous polymer in a diagnostic assay, (2007), American Laboratory, 39 (14), 35-37.

3) A. Z. Haghighi and Robert Wei, Measurement of superoxide dismutase in erythrocytes and whole blood using iodonitrotetrazolium violet, (1998), Anal. Lett., 31 (6), 45.

4) Ali Zendedel Haghighi and Kirk R. Maples, On the Mechanism of the In- hibition of Glutamine Synthetase and Creatine Phosphokinase by Methion- ine Sulfoxide,(1996), Journal of Neuroscience Research, 43, 107-111. 5) Ali Zendedel Haghighi and Martha K. Cathcart, Subtractive antibody to a human immunosuppressive lymphokine affinity isolates a suppressive factor and blocks its function, (1992), Immunopharmacology, 24, 65-76. 6) Ali Zendedel Haghighi, Study on the release of enzymes from the human Polymorphonuclear Leukocytes and development of Superoxide Dismutase assay by p-Iodonitrotetrazolium Violet, (1991), Ph.D. dissertation, Cleveland State University.

7) Thomas I. Pynadath and Ali Zendedel Haghighi, Inhibition of Thrombox- ane A2 synthesis in rats treated with Phenobarbital, (1987), Prostaglandins Leukotrienes and Medicine, 28, 61-71.

8) Ali Zendedel Haghighi and Thomas I. Pynadath, Stimulation of Prostacyclin synthesis in rats treated with Phenobarbital, (1987), Prostaglandins Leukotrienes and Medicine, 28, 267-275. 9) Ali Zendedel Haghighi, Effect of Phenobarbital on low and high density lipoproteins, Thromboxane A2 and Prostacyclin, (1986), M.S. thesis, Kent State University.

10) Hiran M. Data and Ali Zendedel Haghighi, Methylmercuric chloride and serum cholesterol level in Bluegill (Lepomus Macrochirus), (1986), Bull. Environ. Contam. Toxicol., 36, 181-185.

11) Hiran M. Data, S.B. Lall and Ali Zendedel Haghighi, Methyl mercury induced changes in the serum proteins of Bluegills-Lepomus Macrochirus

(Teleostel), (1983), Ohio J. Sci., 83, 119-122.

Papers Presented:

1) Effect of Neutrophil immune response products, proteases and reactive oxygen species, on lens proteins. B.C. Bruno, A. Zendedel Haghighi, T.P. Margolis, S.D. McLeod (2002). The Association for Research in Vision and Opthalmology, 2002 Annual Meeting.

2) Effect of UV light and oxidative damage on the formation of Cataract. A. Zendedel Haghighi, B.W. Woodward (2001), The Association for Research in Vision and Opthalmology, 2001 Annual Meeting, p. S454. 3)On the mechanism of redox reactions in cornea, in the presence or absence of laser treatment. A. Zendedel Haghighi, B.W. Woodward, (2000), The As- sociation for Research in Vision and Ophthalmology, 2000 Annual Meeting, p.S466.

4)A novel and simple procedure for the detection of circulating immune complexes, formed due to excess food allergens in serum. N.T. Eldredge, V. Marinkovich, A. Zendedel Haghighi, (1999), The XVIIIth Conggress of the European Academy of Allergology and Clinical Immunology, P411. 5)Presence of a 97 kDa allergen in Mite, Dermatophagoides farinae. E. Fashandi, N. Eldredge, C. Brown and A. Zendedel Haghighi, (1997), In- ternational Association of Allergology and Clinical Immunology, ICACA XVI, Supplement No. 4, p. 140.

6) The use of Capillary Electrophoresis for Allergen Extract Standardization, Zychlinsky E., Innerst D., Millward V., Zendedel Haghighi A., Eldredge N., Brown C., (1995), The Second Asian Pacific Congress of Allergology and Clinical Immunology, p. 3.

7) Preparation of a subtractive antibody to hybridoma suppressor factor. A. Zendedel-Haghighi and M. K. Cathcart, (1991), The society for Leukocyte Biology/ Leukocyte Culture Conference joint meeting, supplement 2, p. 103. 8) On the determination of Superoxide Dismutase by Iodonitrotetrazolium Violet. A. Z. Haghighi and R. Wei, (1990), American Society for Biochem- istry and Molecular Biology/ The American Association of Immunologists joint meeting, Vol. 4, p. A2304.

9) Release of proteins from the human PMNs treated with PMA or cyclochalasin B/ FMLP. A. Z. Haghighi and R. Wei, (1989), Federation of American Societies for Experimental Biology, Vol. 3, p. A322. 10) Characteristics of intermediate density lipoproteins. Jan J. Opplt and Ali Z. Haghighi, (1987), American Association for Clinical Chemists, Vol. 33, p. 896.

11)Phenobarbital decreases Thromboxane synthesis and increases Prostacyclin synthesis in rats. Thomas I. Pynadath and Ali Z. Haghighi,

(1985), Federation of American Societies for Experimental Biology, Vol. 44, p. 489.

12) Effect of Phenobarbital treatment on serum lipoproteins in rats. Thomas I. Pynadath and Ali Z. Haghighi, (1984), Federation of American Societies for Experimental Biology, Vol. 43, p. 1530.

13) Changes in liver plasma membrane composition of cholesterol-fed rats. Thomas I. Pynadath, Ali H. Zendedel and Brian P. Maloney, (1982), Federa- tion of American Societies for Experimental Biology, Vol. 41, p. 1613. Patent Applications:

1) Compositions and methods for stabilizing modified tissue (# 09559933) 2) Use of dry chemistry to reduce the cost of reagents in a chemical or bio- chemical assay (# SV01268)

3) Apparatus and method for shrinking collagen (#200********) 4) Apparatus and method for modifying dermal collagen (#60271093) 5) Compositions and methods for the treatment of intrinsic and extrinsic skin aging changes (#60715716)

6) Methods and compositions utilizing metal cations for treatment of intrin- sic & extrinsic skin aging changes (#60718534)

7) Methods and composition for generation of reactive oxygen species

(#60921490)

8) Degradation of biomass by metal-catalyzed peroxide oxidation (# 61355392)

References:

References will be provided upon request.

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