Michal Machnicki

**** ******** **

Glendale CA 91208

email: adrei7@r.postjobfree.com

phone: 818-***-****

Work Experience

KitePharma August 2021 to current

Research Scientist and Laboratory Technician

Working with Analytical Development Team

Familiar with multiplex/tetraplex ddPCR (BioRad QX200 – two color and four targets) and RT-ddPCR,

QIACube semi-automated RNA/DNA isolation

Statistical analysis with JMP 15 (e.g. DoE, ANOVA, Jacknife outliers, new method validation)

ELN (Bovia)

Familiar with MS Office

UCSD November 2020 to July 2021

Research Scientist and Laboratory Technician

Started multiplex high throughput qPCR laboratory

PE pipeline for isolation of RNA and PCR amplification from COVID-19

Actively searched for and identified potential mutations detection by sequencing.

NGS library preparation

UCLA January 2006 to November 2020

Laboratory and Research Experience

Developed and applied tools for investigation of stroke and stroke recovery.

Worked independently without requiring supervision

Introduced several new techniques in the lab: lentivirus based miRNA (Invitrogen Gatevway), Gibson Assembly, In Fusion, Golden Gate CRISPR vector generation.

Experienced in Long Range PCR standard PCR and RT-PCR, TaqMan

Familiar with AAV generation (different serotypes) and production for in vitro and in vivo (high concentration) titers

Familiar with virus purification and titration

Experienced in gene isolation (from BACs) and cloning for functional analysis in vivo

Experienced in cell culture, and establishing transient and stably transfected cell lines

Familiar with lipid based transfection and electroporation

Familiar with gene chip Affymetrix and Illumina platforms

Experienced in Laser Captured Microdissection

Experienced in RNA amplification for gene chip application (NuGen Ovation)

Experienced in RNA/DNA quantification (NanoDrop, Qubit, Agilent Bioanalyzer RNA 6000 pico)

Familiar with Western blot and ELISA

Familiar with protein isolation

Management and Mentorship:

Trained students in cell culture, flow cytometry and molecular biology techniques.

Developing knowledge of biosafety

Helped writing grant proposals and designing new experiments that will better answer project goals

Provided troubleshooting and helping design experiments solving technical issues other lab members

Medical College of Georgia, Institute of Molecular Medicine and Genetics September 1997 to December 2005

Post-Doctoral Fellow February 2002 to December 2005

Investigated the source of the cells populating embryonic heart tracing labeled cells on their way to the heart. At different stages cells were isolated and sorted by flow cytometry and used for the detection of genes of interest

Performed immunocytochemistry, library construction, in situ hybridization on tissue sections and whole mount

Isolated and purified total RNA and poly(A)RNA as well as DNA

Performed library construction

Provided DNA/RNA electrophoresis and designed primers

Performed sequencing and DNA cloning

Utilized TUNEL method (apoptotic cell death analysis)

Performed flow cytometry cell sorting, and cell analysis

Analyzed data using records from Gen Bank using GCG, FASTA, VectorNTI DNAstar software to map genomic DNA loci around known genes, identifying functional motifs

Post-Doctoral Fellow September 1998 to February 2002

Worked on genetic regulation of early heart development using knockout mice to investigate the role of specific genes in the early stages of embryonic heart.

Post-Doctoral Fellow September 1997 to August 1998

Worked with the mouse model of LCMV virus ( lymphocytic choriomeningitis virus) looking at T cell receptor repertoire in vivo.

Examined the regulation of virus specific CD8 T cell responses during chronic LCMV infection of mice and how virus can escape immune response.

Analyzed T cell receptor sequences in LCMV viral infections in the mouse model

Neurological Sciences Institute - Portland, OR January 1995 to December 1997

Post-Doctoral Fellow

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy – PL September 1988 to April 1994

Graduate Research Assistant

Education

PhD in Molecular Biology

Institute of Immunology and Experimental Therapy 1994

Research

Institute of Immunology and Experimental Therapy - Wroclaw, PL 1990

M.S. in Immunology

Academy of Medicine - Wroclaw, PL 1988

B.S. in Pharmacy

Wroclaw Medical School 1987

Skills

ELISA

Western Blot

Molecular Biology

Microscopy

Transfection

Aseptic Technique

Microbiology

Immunohistochemistry

Laboratory Experience

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