Glendale Ca Research Assistant
Resume:
Michal Machnicki
Glendale CA 91208
email: ***.******@****.***
phone: 818-***-****
Work Experience
KitePharma August 2021 to current
Research Scientist and Laboratory Technician
Working with Analytical Development Team
Familiar with multiplex/tetraplex ddPCR (BioRad QX200 – two color and four targets) and RT-ddPCR,
QIACube semi-automated RNA/DNA isolation
Statistical analysis with JMP 15 (e.g. DoE, ANOVA, Jacknife outliers, new method validation)
ELN (Bovia)
Familiar with MS Office
UCSD November 2020 to July 2021
Research Scientist and Laboratory Technician
Started multiplex high throughput qPCR laboratory
PE pipeline for isolation of RNA and PCR amplification from COVID-19
Actively searched for and identified potential mutations detection by sequencing.
NGS library preparation
UCLA January 2006 to November 2020
Laboratory and Research Experience
Developed and applied tools for investigation of stroke and stroke recovery.
Worked independently without requiring supervision
Introduced several new techniques in the lab: lentivirus based miRNA (Invitrogen Gatevway), Gibson Assembly, In Fusion, Golden Gate CRISPR vector generation.
Experienced in Long Range PCR standard PCR and RT-PCR, TaqMan
Familiar with AAV generation (different serotypes) and production for in vitro and in vivo (high concentration) titers
Familiar with virus purification and titration
Experienced in gene isolation (from BACs) and cloning for functional analysis in vivo
Experienced in cell culture, and establishing transient and stably transfected cell lines
Familiar with lipid based transfection and electroporation
Familiar with gene chip Affymetrix and Illumina platforms
Experienced in Laser Captured Microdissection
Experienced in RNA amplification for gene chip application (NuGen Ovation)
Experienced in RNA/DNA quantification (NanoDrop, Qubit, Agilent Bioanalyzer RNA 6000 pico)
Familiar with Western blot and ELISA
Familiar with protein isolation
Management and Mentorship:
Trained students in cell culture, flow cytometry and molecular biology techniques.
Developing knowledge of biosafety
Helped writing grant proposals and designing new experiments that will better answer project goals
Provided troubleshooting and helping design experiments solving technical issues other lab members
Medical College of Georgia, Institute of Molecular Medicine and Genetics September 1997 to December 2005
Post-Doctoral Fellow February 2002 to December 2005
Investigated the source of the cells populating embryonic heart tracing labeled cells on their way to the heart. At different stages cells were isolated and sorted by flow cytometry and used for the detection of genes of interest
Performed immunocytochemistry, library construction, in situ hybridization on tissue sections and whole mount
Isolated and purified total RNA and poly(A)RNA as well as DNA
Performed library construction
Provided DNA/RNA electrophoresis and designed primers
Performed sequencing and DNA cloning
Utilized TUNEL method (apoptotic cell death analysis)
Performed flow cytometry cell sorting, and cell analysis
Analyzed data using records from Gen Bank using GCG, FASTA, VectorNTI DNAstar software to map genomic DNA loci around known genes, identifying functional motifs
Post-Doctoral Fellow September 1998 to February 2002
Worked on genetic regulation of early heart development using knockout mice to investigate the role of specific genes in the early stages of embryonic heart.
Post-Doctoral Fellow September 1997 to August 1998
Worked with the mouse model of LCMV virus ( lymphocytic choriomeningitis virus) looking at T cell receptor repertoire in vivo.
Examined the regulation of virus specific CD8 T cell responses during chronic LCMV infection of mice and how virus can escape immune response.
Analyzed T cell receptor sequences in LCMV viral infections in the mouse model
Neurological Sciences Institute - Portland, OR January 1995 to December 1997
Post-Doctoral Fellow
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy – PL September 1988 to April 1994
Graduate Research Assistant
Education
PhD in Molecular Biology
Institute of Immunology and Experimental Therapy 1994
Research
Institute of Immunology and Experimental Therapy - Wroclaw, PL 1990
M.S. in Immunology
Academy of Medicine - Wroclaw, PL 1988
B.S. in Pharmacy
Wroclaw Medical School 1987
Skills
ELISA
Western Blot
Molecular Biology
Microscopy
Transfection
Aseptic Technique
Microbiology
Immunohistochemistry
Laboratory Experience
Contact this candidate