Air package handler.. Restaurant utilities
Resume:
STEVEN BOWEN
*********@*****.***
Raleigh, NC 27603
SUMMARY
My general area of expertise is in microbiology, and molecular genetics, with emphasis on gene regulation. Throughout my career, I have endeavored, and often with success, to make a positive impact on the scientific research goals of various biological research laboratories. It is rewarding to advance the knowledge and understanding of such scientific inquiry.
EXPERIENCE
Utility Worker - Red Lobster, Raleigh, NC
Oct 2018 - present
Job duties include operation of dish machine, washing and sanitizing all dishes, utensils, pots, pans, and food preparation equipment. Assists in maintaining cleanliness of restaurant, including both front and back of house. Assists in receiving and stocking of inventory, and in the opening or closing of restaurant. Contributes to food preparation of the bread (those famous cheddar biscuits) and side orders of rice, potatoes, and seasoned greed beans. Completion of other tasks when needed, including rolling of silverware. Part time position.
Air Package Handler – UPS, Morrisville, NC
Dec 2017- present
Primary responsibilities include unloading of commerce from cargo jets to route trucks, vans, and feeder planes, in the coordinated efforts to ensure timely delivery of packages to final destinations. Part time position.
Meat/Seafood Shoppe Clerk – Lowes Foods, Raleigh NC
Mar 2018 – Sept 2018
Duties and responsibilities were met by providing excellent customer service including aiding guests in choosing high-quality beef, chicken, pork, and seafood products, preparation of foods, maintaining a clean and functional working environment, pricing merchandise, and keeping the presentation case and aisle fully rotated and stocked. Part time position.
Research Associate – Department of Crop Science, North Carolina State University, Raleigh, NC
Dec 2001- Dec 2014
Applied the techniques of molecular biology toward identification and characterization of genes in tobacco to introduce harm reduction traits, as well as other genes of potential agronomic value.
Duties and job responsibilities included the generation and management of large data sets such as sequencing and micro-array data, development, propagation, and analysis of transgenic tobacco plants, alkaloid analysis, molecular marker assays for the screening of large sets of tobacco plants possessing targeted gene modifications, and the care and cultivation of tobacco plants in the laboratory, greenhouse, and field.
Skills acquired beforehand and further developed, and including new ones, consisted of PCR, Southern, Northern, and Western analysis, Sanger and next generation sequencing, development and use of DNA molecular markers, genotyping, epigenetic analysis, and gene expression studies.
A primary objective was achieved in the ongoing efforts relating to harm reduction in tobacco use. The functional development of a non-GM approach through mutational breeding of tobacco to introduce stable knockout mutations in three key genes that encode nicotine N-demethylase activity in select cultivars was accomplished. Such plants were shown to have a dramatic reduction in one of the known tobacco-specific nitrosamines, which are recognized as potent carcinogens.
Postdoctoral Research Fellow – Department of Food, Bioprocessing, and Nutrition, North Carolina State University, Raleigh, NC
May 2001- Nov 2001
Research relating to the study of virulence, molecular epidemiology, and genetics of the foodborne pathogen, Listeria monocytogenes.
Research Associate – Department of Microbiology, North Carolina State University, Raleigh, NC
Nov 1997- April 2001
Investigated the biosynthesis and biological roles of Superoxide Dismutases (SODs) and Hydroperoxidases, key enzymes involved in combating cellular oxidative stress. Primary focus was research on gene regulation of SODs and Oxidative Stress Regulons in Escherichia coli and Streptococcus thermophilus.
Postdoctoral Research Fellow – Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA
Aug 1994- Feb 1997
Employed antisense RNA technology to inhibit Hepatitis B virus gene expression in cell culture by gene transfer as an approach to block immune recognition and pathogenic sequelae. The retroviral-mediated antisense gene transfer combined with liver (or hepatocyte) transplant could provide a molecular targeting approach for treating chronic hepatitis patients.
Postdoctoral Research Fellow – Department of Plant Molecular Biology, Tsukuba Science City, Japan
Feb 1993- Feb 1994
Exploited the natural plant DNA virus, Geminivirus, as proof of concept for use in various plant genetic manipulations such as gene targeting.
EDUCATION
Doctor of Philosophy – PhD (1993) Department of Microbiology, North Carolina State University, Raleigh, NC
Master of Science – MS (1988) Department of Food Science, North Carolina State University, Raleigh, NC
Bachelor of Science – BS (1983) Food Science, North Carolina State University, Raleigh, NC
Associate's Degree – (1981) Brevard College, Brevard, NC
SELECT SKILLS
Basic molecular biology techniques
Protein extraction, isolation, and characterization
Molecular cloning
Gene expression studies including protein and gene fusions
Genetic transfection and transformation techniques in mammalian, plant, and bacterial cells
Tissue culture techniques
Flow cytometry
RNAi technology
Acquisition and management of large biological data sets
Antigen assays (ELISA)
Generation of transgenic plants
Use of next generation machinery for DNA manipulations and analysis
Generation and development of various DNA molecular markers
Traditional, high-throughput, and next generation sequencing
Familiar with various enzymatic assays
PUBLICATIONS
Lewis RS, Lopez HO, Bowen SW, Andres KR, Steede WT, and Dewey RE. (2015) Transgenic and mutation-based suppression of berberine bridge enzyme-like (BBL) gene family reduces alkaloid content in field-grown tobacco. PloS One; 10(2):e0117273.
Li D, Lewis RS, Jack AM, Dewey RE, Bowen SW, and Miller RD. (2012) Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2, and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco. Mol. Breeding 29(3):589-599.
Lewis RS, Bowen SW, Keogh MR, and Dewey RE. (2010). Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tobacum L.: functional characterization of the CYP82E10 gene. Phytochemistry 71(17-18):1988-1998.
Chakrabarti M, Bowen SW, Coleman NP, Meekins KM, Dewey RE, and Siminszky B. (2008) CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway. Plant Mol. Biol. 66(4):415-427.
Gavilano LB, Coleman NP, Bowen SW, and Siminszky B. (2007) Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco. J. Biol. Chem. 282(1):249-256.
Siminszky B, Gavilano LB, Chakrabarti M, Dewey RE, and Bowen SW. (2007) Evolution of nicotine N-demethylase genes and their use in reducing nornicotine levels in tobacco. Recent Adv. Tob. Sci. 33:27-38.
Siminszky B, Gavilano LB, Bowen SW, and Dewey RE. (2005) Conversion of nicotine to nornicotine in Nicotiana tobacum is mediated by CYP82E4, a cytochrome P450 monooxygenase. Proc. Natl. Acad. Sci. USA. 102(41):149**-*****.
Andrus JM, Bowen SW, Klaenhammer TR, and Hassan HM. (2003) Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus A054. Arch. Biochem. Biophys. 420(1):103-113.
Tung FY, Bowen SW. (1998) Targeted inhibition of hepatitis B virus gene expression: a gene therapy approach. Front. Biosci. 3:a11-5.
Bowen SW, and Hassan HM. (1993) Characterization of cis-acting regulatory mutations causing anaerobic expression of the sodA gene in Escherichia coli. Arch. Biochem. Biophys. 302(2):372-379.
Bowen SW, and Hassan HM. (1988) Induction of manganese-containing superoxide dismutase in Escherichia coli is independent of the oxidative stress (oxyR-controlled) regulon. J. Biol. Chem. 263(29):148**-*****.
U.S. PATENTS ISSUED
U.S. Patent 9,228,195 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Jan. 5, 2016) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.
U.S. Patent 9,228,194 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Jan. 5, 2016) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.
U.S. Patent 9,187,759 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Nov. 17, 2015) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.
U.S. Patent 8,124,851 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Feb. 28, 2012) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.
U.S. Patent 7,884,263 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Feb. 8, 2011) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.
The above patents show the compositions and methods for reducing the level of nornicotine and N'-nitrosonornicotine (NNN) in Nicotiana plants. Methods find use in production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.
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