JOHN L. CUDDIHEE

** ***** ****, *******, **

214-***-****

adr0ce@r.postjobfree.com

Objective

A position in the field of biotechnology manufacturing and engineering.

I earned an Advanced Certificate in Biotechnology in May 2020.

Highlights of Skills and Qualifications

Detail-oriented multidisciplinary chemist/engineer with strong background in chemistry and biology. Passionate about stable isotope chemistry and its applications

Experienced in the safe handling and mixing of a wide variety of chemicals including fuels, oxidizers, acids, bases, and biopharmaceuticals

Experienced in Good Manufacturing Practice (cGMP) and Quality Assurance/Quality Control procedures and regulatory compliance including cGMP regulations in 21CFR Parts 210 and 211 for the manufacturing, processing, packing, or holding of drugs and pharmaceuticals

Excellent mentor and communicator with supervisory skills and experience

Biomanufacturing of proteins using bacteria, mammalian, and yeast cells:

oExperienced with aseptic manufacturing techniques and Biological Safety Cabinets

oGrowth of mammalian cell cultures (CHO) in process-controlled bioreactors

oDocumentation guidelines of cGMP

oFilling out batch records and log books

oEnvironmental monitoring for particle counts and viable microorganisms

oProtein purification using filtration and chromatography

oEvaluation for contamination using Gram stain and LAL

Excellent laboratory skills and tools including:

oCurrent Good Laboratory Practice (cGLP)

oEnvironmental Monitoring in Clean Room Environments

oDNA Extraction and Isolation

oDNA Fingerprinting

oSouthern Blotting

oReal Time Quantitative Polymerase Chain Reaction (qPCR)

oIsolation and Purification of Proteins with Column and Batch Chromatography

oIon Exchange and Gel Filtration Chromatography

oSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

oELISA for quantification of proteins

oOperation of lab equipment including pumps, UV detectors, meters, and familiarity with process-control software

oPreparation and editing of SOPs

Recent Previous Employment

Technical Manufacturing Chemist (2021-2022)

Actalent Inc. (Under Contract to Abbott Diagnostics)

Westbrook, ME

Preparation of reagents and antibody conjugate solutions applied to nitrocellulose membranes in support of the manufacture of COVID-19 test kits. Experienced with preparation of heavy-metal nanoparticle solutions and antibody solutions. Also responsible for safety training of staff for the manufacture and filtration of large quantity batches of a variety of buffer solutions.

Enumerator (2020)

United States Census – Department of Commerce

New Hampshire Seacoast

Senior Consultant (2001-2019)

TERRACON CONSULTANTS INC

Dallas, TX and Manchester, NH

Engineering design and project management associated with a variety of environmental engineering projects including bioremediation and water treatment design. Specialized in manipulating subsurface groundwater conditions by injecting a variety of amendments (including specialized bacterial cultures) to promote enhanced biodegradation of groundwater and soil contaminants. Extensive experience with water chemistry and treatment.

Education History

Issuing Institution

Location

Degree/Certificate

Course of Study

Great Bay Comm. College

University of Houston

Portsmouth, NH

Houston, TX

May 2020

Master's Degree

Biotechnology

Env. Engineering

Rice University

Houston, TX

Master's Degree

Isotope Geochemistry

Cornell University

Ithaca, NY

Bachelor's Degree

Geology (Chemistry Concentration)

Occupational Licenses, Certificates and Training

Certification Title

Issuing Organization

Water Works Operator

New Hampshire

Professional Engineer

Florida

Professional Engineer

Texas

Professional Engineer

New Hampshire

Contact this candidate