Great Bay Water Works
Resume:
JOHN L. CUDDIHEE
************@*****.***
Objective
A position in the field of biotechnology manufacturing and engineering.
I earned an Advanced Certificate in Biotechnology in May 2020.
Highlights of Skills and Qualifications
Detail-oriented multidisciplinary chemist/engineer with strong background in chemistry and biology. Passionate about stable isotope chemistry and its applications
Experienced in the safe handling and mixing of a wide variety of chemicals including fuels, oxidizers, acids, bases, and biopharmaceuticals
Experienced in Good Manufacturing Practice (cGMP) and Quality Assurance/Quality Control procedures and regulatory compliance including cGMP regulations in 21CFR Parts 210 and 211 for the manufacturing, processing, packing, or holding of drugs and pharmaceuticals
Excellent mentor and communicator with supervisory skills and experience
Biomanufacturing of proteins using bacteria, mammalian, and yeast cells:
oExperienced with aseptic manufacturing techniques and Biological Safety Cabinets
oGrowth of mammalian cell cultures (CHO) in process-controlled bioreactors
oDocumentation guidelines of cGMP
oFilling out batch records and log books
oEnvironmental monitoring for particle counts and viable microorganisms
oProtein purification using filtration and chromatography
oEvaluation for contamination using Gram stain and LAL
Excellent laboratory skills and tools including:
oCurrent Good Laboratory Practice (cGLP)
oEnvironmental Monitoring in Clean Room Environments
oDNA Extraction and Isolation
oDNA Fingerprinting
oSouthern Blotting
oReal Time Quantitative Polymerase Chain Reaction (qPCR)
oIsolation and Purification of Proteins with Column and Batch Chromatography
oIon Exchange and Gel Filtration Chromatography
oSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
oELISA for quantification of proteins
oOperation of lab equipment including pumps, UV detectors, meters, and familiarity with process-control software
oPreparation and editing of SOPs
Recent Previous Employment
Technical Manufacturing Chemist (2021-2022)
Actalent Inc. (Under Contract to Abbott Diagnostics)
Westbrook, ME
Preparation of reagents and antibody conjugate solutions applied to nitrocellulose membranes in support of the manufacture of COVID-19 test kits. Experienced with preparation of heavy-metal nanoparticle solutions and antibody solutions. Also responsible for safety training of staff for the manufacture and filtration of large quantity batches of a variety of buffer solutions.
Enumerator (2020)
United States Census – Department of Commerce
New Hampshire Seacoast
Senior Consultant (2001-2019)
TERRACON CONSULTANTS INC
Dallas, TX and Manchester, NH
Engineering design and project management associated with a variety of environmental engineering projects including bioremediation and water treatment design. Specialized in manipulating subsurface groundwater conditions by injecting a variety of amendments (including specialized bacterial cultures) to promote enhanced biodegradation of groundwater and soil contaminants. Extensive experience with water chemistry and treatment.
Education History
Issuing Institution
Location
Degree/Certificate
Course of Study
Great Bay Comm. College
University of Houston
Portsmouth, NH
Houston, TX
May 2020
Master's Degree
Biotechnology
Env. Engineering
Rice University
Houston, TX
Master's Degree
Isotope Geochemistry
Cornell University
Ithaca, NY
Bachelor's Degree
Geology (Chemistry Concentration)
Occupational Licenses, Certificates and Training
Certification Title
Issuing Organization
Water Works Operator
New Hampshire
Professional Engineer
Florida
Professional Engineer
Texas
Professional Engineer
New Hampshire
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