Kumar Subramani Page * of **

CURRICULUM VITAE

Education and Institution

Dr. KUMAR SUBRAMANI, Ph.D.

Assistant Staff Scientist

Cardiovascular Biology Program,

Oklahoma Medical Research Foundation (OMRF),

825, Northeast, 13

th

Street,

Oklahoma City, Oklahoma, 73104.

E-mail: adr05l@r.postjobfree.com

Phone: +1-706-***-****.

Residential address:

208, NW 54

th

Drive, Apt# B

Oklahoma City,

Oklahoma, 73118.

VISA Type:

Green Card Holder (Permanent Resident)

INSTITUTION AND LOCATION DEGREE MM/YY FIELD OF STUDY Cardiovascular Biology Program,

Oklahoma Medical Research Foundation (OMRF)

825 Northeast 13th Street

Oklahoma City, Oklahoma, 73104.

Assistant

Staff Scientist

10/2020 –

present

Cardiovascular Biology

Department of Internal Medicine,

Texas Tech University Health Sciences Center,

Lubbock, Texas, USA.

Post-doctoral

Research

Associate

11/2018 –

9/2020

Cardiovascular Biology

Medical College of Georgia,

Augusta University, Augusta, Georgia, USA.

Post-doctoral

Fellow

11/2014 -

11/2018

Trauma Hemorrhagic

shock and Sepsis

Annamalai University, India

Ph.D.

01/2014

Biochemistry

(Cardiovascular

Biology)

Annamalai University, India

M.Phil.

11/2009

Biochemistry

(Cardiovascular

Biology)

Thiruvalluvar University

M.Sc.

4/2008

Biochemistry

University of Madras

B.Sc.

4/2005

Biochemistry

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Professional Experience

Assistant Staff Scientist Current Research Work: Cardiovascular Pharmacology

Aortic stenosis occurs when the heart's aortic valve narrows. Aortic Stenosis and Heart failure are serious medical problems primarily affecting the elderly that can lead to death and as there are no effective medical treatments other than valve replacement or implantation with left ventricular assist device surgically, respectively. TGF-β 1 is a potent cytokine (potent inducer of fibrosis) and we have shown that it can be released from platelets and subsequently becomes activated by shear stress in vitro via thiol-disulfide exchange mechanism and that mice deficient in platelet TGF-β1 are protected from developing fibrosis in the heart when they undergo surgery to induce high shear stress. My current project in understanding the role of NAC and Galunisertib in mitigating age-related aortic stenosis is progressing toward finding a long-term treatment method in humans. Currently, I am working on aortic stenosis and ascending aortic constriction (AAC) models and the role of Galunisertib on AAC in heart fibrosis and shear stress activation of TGF-β1 mouse models and cell line. Overall, in our studies will illuminate and guide the development of potential interventions to prevent aortic stenosis progression as well as diagnostic tools to detect early complications in post-left ventricular assist device implantation heart failure patients.

Past Post-Doctoral Research Work: Cardiovascular Pharmacology The goal of previous research work is to better understand the cellular and molecular regulation of cardiovascular function in health and disease. Moreover, hypertension is directly implicated in various CVD states, including stroke, ischemic disease, and peripheral vascular disease. In this research work understanding the role of thioredoxin in mitigating age-related hypertension is progressing toward finding a long-term treatment method in human. In this work was arterial stiffness markers changes in young and aged and baboon models and role of thioredoxin on Angiotensin II induced hypertension in arterial stiffness markers on HASMC cell line. In this research project requires my participation toward reaching the goal of finding a cure for hypertension. Past Post-Doctoral Research Work: Hemorrhagic Shock and Sepsis Severe hemorrhage causes whole body hypoxia, multiple organ dysfunction and death. Fluid resuscitation is employed to restore organ perfusion and reoxygenation following hemorrhagic injury. Reoxygenation causes oxidative damage and cell and organ injury and there is a need to develop better resuscitation methods to reduce cell injury following hemorrhage. My past post- Kumar Subramani Page 3 of 10

doctoral research projects that include resveratrol, EVs, dichloroacetate and niacin in treatment with hemorrhagic injury mice and rat models. I was investigated the signaling mechanisms in the microcirculation during inflammation, trauma hemorrhage and sepsis.

Ph.D. and M.Phil. Research Work: Cardiovascular Pharmacology My M.Phil. & Ph.D. research works are focused on the study of biochemical and molecular mechanisms of naturally occurring phenolic phytochemical antioxidants medicated cardioprotective effect and left ventricular function in experimental male albino rats in the platform gene and protein expression levels in cardiovascular treatments.

Doctoral Research: Thesis entitled- “Evaluation of cardioprotective effects of syringic and vanillic acid in L-NAME induced hypertensive rats”. Master of Philosophy Research: Thesis entitled- “Effect of piperine a natural alkaloid in L-NAME induced hypertensive rats.

Technical expertise:

Animal handling experience

Rats handling for 10 years’ experience.

Transgenic and knockout mice handling for 8 years’ experience.

Baboon tissue samples handling for 2 years’ experience. Animal necropsy experience

Rats and mice necropsy for 13-14 years’ experience.

Baboon necropsy for 2 years’ experience.

Dose administration

Oral gavage for rats and mice.

Intraperitoneal (i.p), Intramuscular, tail vein and Subcutaneous). Blood collection

Cardiac puncture.

Retro orbital.

Surgical models for cardiovascular biology:

Aortic Stenosis model.

Ascending aortic construction (AAC) in mice model.

Telemetry surgery in mice model.

Hemorrhagic injury mice and rat model.

Polymicrobial sepsis in mice and rat model.

Uninephrectomy (removal of one kidney) to induce hypertension in Rat and mouse model.

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Cardiovascular hemodynamics

Mastered and excelled in echocardiographic assessment techniques using a cutting-edge high-resolution ultrasound device, Vevo-2100, a gold- standard method used to characterize aortic stenosis and heart failure in mice model.

Ex-vivo isolated Langendorff mouse and rat heart system for cardiac assessment.

Blood pressure measurement by non - invasive tail cuff method.

Myograph Systems are used to measure the physiological function and properties of small arteries from mice.

Histology:

Paraffin embedded and OCT tissues preparation.

Tissues sectioning in microtomes 2- 4years experience (mice, baboon and human).

Immunofluroscence and Immunohistochemistry for 8 years’ experience.

H&E, Masson's trichrome, Alizarin red, Alcian blue staining for 4years experience.

Biochemistry and Molecular biology

Western blot.

Immunoprecipitation.

ELISA.

Genotyping.

Isolation of RNA and DNA, Gene amplification by PCR, RT-PCR, Agarose gel Electrophoresis, siRNA gene knockdown.

Cell biology

Hemavet 950 Analyzer

Analysis in erythrocyte deformability.

Mitochondrial and nuclear fraction isolation from tissues and cell culture samples.

Cell culture

Cell line culture (Human aortic smooth muscle cells, Human aortic endothelial cells, MLEC, HLMVEC and H9C2 cells).

Statistical data analysis

Graph Pad Prism, ANOVA (SPSS), student -t test and Image J. Computer skills

Microsoft, adobe Photoshop and illustrator.

Research Assistantship and Teaching

Guided several undergraduate and postgraduate students in research projects.

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Awards Received

University Research Fellowship (2011-2012).

Membership in Society

Member: Indian Society for Biological Chemist (SBC), India. (2008-2014).

Member: American Heart Association.

Member: American Society for Biochemistry and Molecular Biology.

Member: Shock Society.

Scientific Journals Review Panel

Naunyn-Schmiedeberg’s Archives of Pharmacology.

Scientific Report

Journal of King Saud University – Science.

Cardiology Research and Practice.

Editorial board member: EMS Surgery Journal.

Board member: International Journal of Pharmaceutical Research.

Editorial Board (International): International Journal of Pharmacy Research & Technology (IJPRT).

Publications

1. Sandra Gostynska, Thamizhiniyan Venkatesan, Kumar Subramani, Brienne Cortez, Amanda Robertson, Sandeep Subramanian, Pratibha Dube, Jasimuddin Ahamed. Megakaryocyte/platelet-derived TGF-β1 inhibits megakaryopoiesis in bone marrow by regulating thrombopoetin production in liver. Blood Advance. 2022 Jun 14;6(11):3321-3328. doi: 10.1182/bloodadvances.202*******. (Impact factor: 6.799) 2. Sandeep Subrahmanian, Rohan Varshney, Kumar Subramani, Brennah Murphy, Sean Woolington, Jasimuddin Ahamed. N-acetylcysteine inhibits aortic stenosis progression in a murine model by blocking shear-induced activation of platelet latent TGF-β1. Antioxidant Redox signaling. 2021 Oct 8. doi: 10.1089/ars.2021.0037 (Impact factor: 8.401). 3. Xiaogang Chu, Kumar Subramani, Bobby Thomas, Alvin V. Terry, Sadanand Fulzele, Raghavan Pillai Raju. Reparative effect of juvenile plasma-derived factors in aging and injury. Aging and Disease. 2022; 13(2): 568–582.

(Impact factor: 6.745).

4. Subramani Kumar, Chu Xiaogang, Warren Marie, Lee Mariah, Lu Sumin, Singh Nagendra, Raju Raghavan. Deficiency of metabolite sensing receptor HCA2 impairs the salutary effect of niacin in hemorrhagic shock. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2019; 1865 (3), 688-695. (Impact factor: 5.187).

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5. Subramani Kumar, Raju Srikrishnan, Chu Xiaogang, Warren Marie, Pandya Chirayu, Hoda Nasrul, Fulzele Sadanand, Raju Raghavan. Effect of plasma- derived extracellular vesicles on erythrocyte deformability in polymicrobial sepsis. International immunopharmacology. 2018; 65, 244-247. (Impact factor: 4.932).

6. Warren Marie

1

, Subramani Kumar

1

, Schwartz Richard, Raju Raghavan.

Mitochondrial dysfunction in rat splenocytes following hemorrhagic shock. Biochim Biophys Acta (BBA)-Molecular Basis of Disease. 2017; Oct;1863(10 Pt B):2526-2533. doi: 10.1016/j.bbadis.2017.08.024. (1equal contribution). (Impact factor: 5.187).

7. Subramani Kumar, Lu Sumin, Warren Marie, Chu Xiaogang, Toque A Haroldo, Caldwell R William, Diamond P Michael, Raju Ragavan. Mitochondrial targeting by dichloroacetate improves outcome following hemorrhagic shock. Scientific Reports. 2017 Jun 1; 7(1):2671. doi: 10.1038/s41598-017-02495-5. (Impact factor: 4.380). 8. Lu Sumin, Aguilar Alex, Subramani Kumar, Poulose Ninu, Ayub Ahmar, Raju Raghavan. Alteration of cytokine profile following hemorrhagic shock. Cytokine. 2016; 81:35-38. doi:10.1016/j.cyto.2016.01.022. (Impact factor: 3.488).

9. Murugesan Saravanakumar, Boobalan Raja, Jeganathan Manivannan, Thangarasu Silambarasan, Pichavaram Prahalathan, Subramanian Kumar, Santosh Kumar Mishra. Oral administration of veratric acid, a constituent of vegetables and fruits, prevents cardiovascular remodelling in hypertensive rats: a functional evaluation. British Journal of Nutrition. 2015; Nov.114(9):1385-94. doi: 10.1017/S0007114515003086. (Impact factor: 3.334).

10. Subramanian Kumar, Pichavaram Prahalathan, Murugesan Saravanakumar, Boobalan Raja. Vanillic acid prevents the deregulation of lipid metabolism, endotheline 1 and up regulation of endothelial nitric oxide synthase in nitric oxide deficient hypertensive rats. European Journal of Pharmacology. 2014; Sep 17. 743, 117-125. (Impact factor:4.432). 11. Subramanian Kumar, Pichavaram Prahalathan, Boobalan Raja. Vanillic acid: a potential inhibitor of cardiac and aortic wall remodeling in L-NAME induced hypertension through upregulation of endothelial nitric oxide synthase. Environmental Toxicology and Pharmacology. 2014; Jul 23;38(2):643- 652. (Impact factor: 3.292).

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12. Subramanian Kumar, Pichavaram Prahalathan, Boobalan Raja. Syringic acid ameliorates L-NAME induced hypertension by reducing oxidative stress. Naunyn-Schmiedeberg’s Archives of Pharmacology. 2012; Dec;385(12):1175-84. (Impact factor: 2.238).

13. Subramanian Kumar, Pichavaram Prahalathan, Boobalan Raja. Antihypertensive and antioxidant potential of vanillic acid, a phenolic compound in L-NAME induced hypertensive rats: A dose dependence study. Redox Report. 2011; 16(5):208-15. (Impact factor:4.412). 14. Subramanian Kumar, Murugesan Saravanakumar, Boobalan Raja. Antihypertensive, Antioxidant and free radical scavenging potential of syringic and vanillic acid, a phenolic compound in L-NAME induced hypertension. In- vivo and in-vitro study. Journal of Pharmacy Research. 2012; 5(5): 2942- 2948. (Impact factor: 0.27).

15. Subramanian Kumar, Murugesan Saravanakumar, Boobalan Raja. Efficacy of piperine, an alkaloidal constituent of pepper on nitric oxide, antioxidants and lipid peroxidation markers in L-NAME induced hypertensive rats. International Journal of Research in Pharmaceutical Science. 2010; 1, 3: 300-307. (Impact factor: 0.60).

16. Pichavaram Prahalathan, Subramanian Kumar, Boobalan Raja. Morin attenuates blood pressure and oxidative stress in deoxycorticosterone acetate-salt hypertensive rats: A biochemical and histopathological evaluation. Metabolism. 2012; 61(8):1087-1099. (Impact factor: 6.513). 17. Pichavaram Prahalathan, Subramanian Kumar, Boobalan Raja. Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study. Asian Pacific Journal of Tropical Biomedicine. 2012; 443-448.

(Impact factor: 1.72).

18. Murugesan Saravanakumar, Subramanian Kumar, Boobalan Raja. Antihyperlipidemic effect of borneol on L-NAME induced hypertensive rats. Journal of Pharmacy Research. 2010; 3(10):2362-2365. (Impact factor: 0.27).

19. Murugesan Saravanakumar, Subramanian Kumar, Boobalan Raja. Antihypertensive and antioxidant potential of borneol-a natural terpene in L-NAME induced hypertensive rats. International Journal of Pharmaceutical and Biological Archive. 2010; 1(3):271-279. (Impact factor: 0.74).

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PROCEEDINGS

1. Kumar S, Sandeep S, Rohan V, Thamizhiniyan V, Brienne C, Pratibha D, Jasimuddin A. Low dose galunisertib halts aortic stenosis progression by inhibiting TGF-β signaling in mice. The Heart Valve society meeting, March 3-5, 2022. (https://heartvalvesociety.org/meeting/program/2022/15.cgi). 2. Xiaogang C, Kumar S, Marie W, Raghavan PR. Innate immune response in acute lung injury following hemorrhagic shock. Journal of Immunology. May 1, 2017; 198 (1 Supplement) 70.9.

3. Kumar S, Sumin L, Marie W, Alex A, Damalie Z; Ham PB, Michal D, Raghavan PR. Role of mitochondria in hemorrhagic injury. Shock. 45, 6: 90- 91, 2016.

4. Kumar S and Raja B. Effect of syringic acid treatment on cardiac remodeling and antioxidant enzymes in L-NAME induced hypertensive rats. Indian Journal of Physiology and Pharmacology. 2011; 55 (5)113-114. Presentations - National/International conferences 1. Xiaogang C, Kumar S, Marie W, Raghavan PR. Presented a poster entitle

“Innate immune response in acute lung injury following hemorrhagic shock.” Journal of Immunology. May 1, 2017, 198 (1 Supplement) 70.9. Washington DC convention center, USA.

2. Kumar S. and Raja B. Presented a paper entitled “Effect of syringic acid treatment on cardiac remodeling and antioxidant enzymes in L-NAME- induced hypertensive rats.” in APPICON 2011, Department of Physiology, and (December 13-17, 2011). All India Institute of Medical Sciences, AIIMS, Ansari Nagar, New Delhi, 110, 029, India.

3. Kumar S. and Raja B. Presented a paper entitled “Efficacy of phenolic compounds, vanillic and syringic acid on blood pressure, antioxidants and lipid peroxidation markers in L-NAME hypertensive rats - A dose dependant study” in UGC, CSIR, ICMR & MoES. International Conference on Natural Products and Biomedical Technology (ICNPBT-2011). Jan. 10-12, 2011. Annamalai University, Tamil Nadu, India.

4. Kumar S. and Raja B. Presented a paper entitled Antihypertensive and antioxidant effect of vanillic acid, a phenolic compound in L-NAME hypertensive rats. National Conference on “Recent trends in phytotherapy research” 21

st

Annual meeting of society of biological chemists (India) Annamalainagar Chapter 05

th

Mar. 2011.

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5. Kumar S. Saravanakumar M, Raja B. Presented a paper entitled “Beneficial effect of piperine a natural alkaloid nitric-oxide, blood pressure and oxidative stress in L-NAME hypertensive rats” in UGC sponsored National Conference on Advances in Biological Sciences (ABS), Dept. Zoology, Annamalai University, Annamalainagar, Tamil Nadu, and Jan. 2010. 6. UGC Sponsored National conference on Molecular and Cellular Mechanism of Diseases: Intervention with Natural Products” & The 22 nd

Annual Scientific

Meeting of Society of Biological Chemists (India), Annamalainagar, Chapter. April 7

th

and 8

th

2012, Annamalai University, Tamil Nadu, India.

7. 21

st

National conference of the Indian Society of Hypertension (BPCON 2011), World Hypertension League and Canada-India Network Society, Rajah Muthiah Medical College, Annamalai University, Annamalainagar, Tamil Nadu, India, 24-25,

th

Sep. 2011.

8. National conference on Explorations of the Natural Bioactive Compounds from the Marine Resources (BIOACTIVA’2011), Department of marine sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu. Feb. 10-11, 2011.

9. International Conference on Natural Products in Health & 21 th

Annual

Conference of the Indian Society for Atherosclerosis Research, Dec. 2008, Annamalai University, Tamil Nadu, India.

10. National conference on “Frontiers in Medical Biochemistry” & The 20 th

Annual

Scientific Meeting of Society of Biological Chemists (India), Annamalai Nagar, Chapter. Feb. 2010, Annamalai University, Tamil Nadu, India. 11. National conference on “Frontiers in Biology” & the 18 th

“Annual Scientific

Meeting of Society of Biological Chemists (India), Annamalainagar, Chapter. Feb. 09

th

Annamalai University, Tamil Nadu, India.

12. 6

th

winter Symposium “Cardiovascular Science.” Jan 10-12, 2008; Christian Medical College, Vellore, Tamil Nadu. India.

13. Intersociety of Biochemistry and Nutrition III Annual National Symposium

“Cancer Today” -07 19

th

Feb.2007 D.K.M. College, Vellore, Tamil Nadu. India. Kumar Subramani Page 10 of 10

WORKSHOPS ATTENDED

Attended in AYUSH, MoES UGC sponsored National Workshop on “Industry Meet to Upgrade Herbal Medicine (AIMHM) - Organized by Department of Biochemistry and Biotechnology” Annamalai University, 8 th

August. 2011.

Participated in “Biotechniques Illumine” a workshop demonstrating techniques in Cellular and Molecular Biology, Organized by the Department of Biochemistry, Christian Medical College, Vellore, on 24 th

Oct. 2007.

Gas Chromatography for one-week training July 30 th

to August 3

rd

2007.

Advanced Training Institute Chennai Government of India Ministry of Labour and Employment Directorate General of Employment and Training. DECLARATION

I do hereby declare that the above-mentioned information given is correct, complete, and true to best of my knowledge and belief. Yours sincerely,

Kumar Subramani

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