Meerut Plant

Kuheli Sen

Contact: 1-314-***-**** (Cell). Email: **********@*****.***

Github: https://github.com/kuhelidsen/data-science-projects

LinkedIn: www.linkedin.com/in/kuhelidsen

Educational Degrees

Master’s in Data Science: Maryville University of St Louis, MO (Dec 2021). GPA: 4.0

Ph.D. (Microbiology, Biotechnology): GBPUA&T. Pantnagar, India (Aug 2005). GPA: 3.6

Master’s (Botany, Biotechnology): GBPUA&T. Pantnagar, India (Aug 2001). GPA: 3.7

Bachelor’s (Zoology, Botany, Chemistry): C.C.S. Univ. Meerut, India (Jun 1999). GPA: 3.8

Certificates

Certificate in Machine Learning: Maryville University of St. Louis (Dec 2021). GPA: 4.0

Certificate of Specialization in Biotechnology: St. Louis Community College. St Louis. MO (Dec 2014). GPA: 4.0

Technical Skills

R Programming

Predictive Modelling

Text Mining

Experimental Design

SQL

Math Modelling

Big Data Analytics

Tableau

Python

Basic Linux Commands

Blockchain

SAS

Machine Learning

Data Visualization

Work Experience

Washington University School of Medicine: Programmer I: Feb 2021 – Present

Statistical Analysis of Phenotypic data of Clinical Trials of patients of Alzheimer’s disease using Python, SAS and SQL and visualization using R and Python

Data Base management using SQL, worked on relational database (File maker Pro) by designing new workflows (forms to submit samples and entering data to the tables automatically) for submission of clinical samples

MOgene LC.: Research Scientist: Aug 2014 – May 2020

Next Generation Sequencing: Manage and execute NGS based projects as well as developed, tested, validated and implemented new genomic technologies and protocols. Prepares reports and send the data to the clients

Preparing DNA libraries using illumina, pacbio and KAPA reagent kits and load them to sequencer for gene sequencing, performing Quality Check for DNA samples using bioanalyzer, tapestation, nanodrop, agarose gel electrophoresis, quantitate DNA using picogreen assay, QPCR and Qubit. Operating Nextseq, Miseq and pacbio sequencers. DNA, RNA and protein isolations. Operating liquid handlers. Uses Microsoft excel, word and power point

Regulated and executed Clinical Diagnostic Laboratory Test (CLIA).

Other Assays: Demonstrated knowledge and experience in QPCR based Assays, Nanostring assays, protein assays and Fluidigm assays

Microarrays: One color and dual color microarray-based gene expression, Comparative Genomic Hybridization of DNA samples by labeling the samples with cyanine dyes and hybridizing them on agilent microarray slides. Scanning the slides, extracting the features using feature extraction software and analyzing the data using genomic workbench software to detect mutation in different DNA samples

Data Analysis: Demultiplexing and file processing on linux based system to send out NGS data to clients. Analyzing RTQPCR, SNP data, microarray data and other QC data analysis

Sigma-Aldrich: Intern: Jun 2014 – Aug 2014

Mammalian Cell Culture techniques: Performed Sterile Techniques and used Biological Safety Cabinet for cell culture techniques, estimated cell count using Hemocytometer and automated cell counter, passing cells and maintaining cells for few months, estimating % confluence of cells, creating minipools of cells in a 96 well plate, cell banking

Molecular techniques: Isolated genomic DNA from plant tissue, bacteria and mammalian cells and estimated concentration, isolated plasmid DNA from E.coli (minipreps and maxipreps), Polymerase Chain Reaction (PCR) and PCR cleanups, transformation and transfection

Lecturer: CMR Institute of Management and Science, Bangalore, India: Oct 2006 – Nov 2007

Microbiology, Biotechnology and Molecular genetics: Theory and Lab classes for B.S. and M.S. students.

For B.S. students (Theory) I taught General Microbiology which includes different types of microflora, classification of bacteria and fungi. Detailed description of gram positive and gram-negative bacteria and different types of fungi

B.S.Lab Class: gram staining of bacteria to differentiate gram positive and gram negative bacteria. Staining and differentiating different types of fungus under microscope

For M.S. students (Theory) I taught Molecular Genetics which includes Different Laws of Genetics, dominant and recessive genes, different hereditary diseases, molecular basics of genetics which includes linkage and crossing over. Molecular description of cell division and different types of syndromes

M.S.Lab Class: DNA-isolation from plants. Plant tissue culture. Different stages of cell division under microscope. Solving genetics problems using Laws of Heredity

PhD Thesis:

‘P’ Solubilization Potential of Plant Growth Promoting Pseudomonas Mutants at Low and Ambient Temperature

Thesis work focused on potential of cold tolerant mutants of Ps. fluorescens strains for improving plant growth and thus promoting organic farming in an eco-friendly environment.

Awards /Fellowship

National Eligibility Test (NET) – 2003

GATE – 2001 (Graduate Aptitude Test in Engineering).

Research Publication

Das, K., Katiyar, V. and Goel, R. (2003) `P’ solubilization potential of plant growth promoting Pseudomonas mutants at low temperature. Microbiol. Res. 158, 359-362.

Abstracts in Conferences

Das, K.; and Sharma. A. 2003. Biocontrol of Plant Pathogens by Rhizobacteria from diseased Rice Plants. International Symposium on “Molecular Toxicology and Environmental Health”. Held at ITRC, Lucknow from Nov. 5-8, 2003. (Abst. ME-2)

Das, K., Kumar, A. and Rao, P.B. 2003. Use of RAPD markers to study genetic diversity in various species of the genus Sonchus, family Asteraceae. National Symposium on “Improving Crop Productivity in an Ecofriendly Envioronment: Physiological and Molecular Approaches” held at G.B.P.U.A. & T., Pantnagar from Oct. 15-17, 2003. (Abst. P/VI/6).

Trainings

Training course on pacbio sequencing and library construction, 2019.

Training course on “Gene Cloning and Expression”, 11-31 August 2003, conducted by NATP.TO E, Veterinary Vaccines Technology held at Indian Veterinary Research Institute, Bareilly.

Workshop on “Online documentation of Plant Biodiversity of India with special reference to Uttaranchal”, conducted by Department of Molecular Biology & Genetic Engineering, G.B.P.U.A. & T. Pantnagar from Jan.10-14, 2004.

Training course on “IPR and WTO issues: Relevance for Uttaranchal” conducted by Patent Information Centre held at G.B.P.U.A. & T., Pantnagar.

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