Microbiology/virology-biotechnology-infectious diseases-biochemistry

CIRRUCULUM VITAE

Name: Nasser Khodai Booran

Date of Last Update: Nov 01, 2021

Mail Address: 126 Lambton Avenue,

Toronto-Ontario, M6n 2S9

Telephone: work. 416-***-****

Personal Information: e –mail: *************@*****.*** and ******.************@********.**

EDUCATION

Degrees, Diplomas, Licensures and Certifications

1997 Visiting scientist, Pasteur Institute

Paris, France *

1996 PhD in Medical Bioscience/Biotechnology, Pasteur Institute

Of Tehran and other Universities/Organizations**

1991 M.Sc., Medical Microbiology, Tehran University of Medical

Sciences

1985 B.Sc.(hon)., Microbiology, University of Tehran

**PhD program was done in Pasteur Institute with collaboration of other academic and industrial Institutions (Tehran University of Medical Sciences, Blood transfusion centre, Genetic Engineering and Biotechnology Centre, National Industrial Research Centre. The PhD transcript was evaluated course by course by World Evaluation Services (WES) in USA and Canada. I ranked #1 in the national exam among more than 500 participants in this Ph.D program.

*First person from Pasteur Institute of Iran who was chosen by Pasteur Institute of Paris Scientific Committee and was granted for bursary from government of France as visiting scientist in the laboratory of the Unite de Programation Moleculaire et Toxicologi Genetique, Biotechnology Dept., Pasture Institute of PARIS-France

Specialty Training:

1987-1998 Medical Parasitology (Practical and Theoretical) Health Faculty, Tehran University of Medical Sciences

1991 Bacterial Genetic (practical and Theoretical) in Pasteur Institute of Iran supervisor Professor G.B. Smirnov

1991-1992 Purification of immunoglobulin from human sera and made SRID kits in Biochemistry Department, Pasteur Institute of Iran

1992 Visiting scientist in GlaxoSmithKline Biologicals, Rixensart, Belgium.From ministry of health, Deputy in Food and Drug.

1992 Animal Tissue Culture by W.H.O. collaborating centre for Reference and Research on Rabies and Viral Derivatives Departments of Pasteur Institute of Iran

1992 Immunology and Molecular Biology of Infectious Diseases (Theoretical and Practical) ELISA, Western Blotting. etc. by W.H.O. Pasteur Institute of Iran

1992 Restriction Enzyme Purification, Molecular Biology Department Pasteur Institute of Iran by Professor V. Rechinskii of Moscow Biotechnology Centre

1994 PCR and its applications. Department of Biotechnology. Pasteur Institute of Iran by Dr. F Kashanchi of NIH, USA

1995 Radiation Protection in Medicine, Atomic Energy Centre of Iran

2001 Second International Course on Infections and Cancer, WHO International Agency for Research on Cancer (IARC) Foundation, Merieux, France

2006 Real-Time PCR training Hospital for Sick Children, Toronto, Canada

2008 Partek RNA and DNA Data Analysis Workshop at The Hospital for Sick Children on June 19 and 20. The Centre for Applied Genomic @ SickKids.

2013 DNA/RNA Sequencing and Genotype Analysis Workshop with Golden Helix. Next Generation Sequencing (NGS) Analysis: Family-based DNA-Sequencing, NGS Analysis: Rare variant analysis in SVS. Toronto, On, at Research Institute of The Hospital for Sick Children

2014 Judge at Sikkids hospital Summer Research (SSuRe) Program Symposium, August, 7 2014; The Hospital for Sick Children.

2014 Research Quality and Risk Management, Medical Oversight in Clinical Trials, August 20, 2014, The Hospital for Sick Children.

2014 Incidental Leadership Workshop, Manage Conflict and Improve Relationship. October 23 & 24, 2014. The Hospital for Sick Children- Research Institute.

Doctoral Thesis

1. Detection of human Herpes Viruses by Polymerase Chain Reaction and its role in Pathophysiology of Atherosclerosis 1996 (PhD thesis)

2. Study of beta hemolytic Streptococci group A in School Children 1991 (MSc thesis)

3. Designing of recombinant vectors and expression of Escherichia coli of recombinant proteins composed of the Maltose Binding Protein from E. coli in which epitopes from the AIDS virus HIV were inserted into different position and their Immunological assay. Pasteur institute of PARIS, France, 1996-97

Appointments/Work Experience

1980-1987 Technician in private medical laboratories- duties included sampling, microbiology, parasitology, serology, hematology, immunology, biochemistry etc.

1987-1991 Technologist in microbiology and chemistry lab, in the Parenteral Solutions Department in Quality Control Lab and line product controller at the Pasteur Institute, Karaj, Iran

1992 Lecturer and Head of Microbiology Lab-

Pasteur Institute of Iran

1992 Established a new molecular genetic lab in the Biochemistry Department for detection of pathogens by nested and multiplex PCR (HCMV, HSV, Toxoplasma)

Pasteur Institute of Iran-Tehran.

1993-1994 Supervised and trained researchers and technologists to establish a new lab for the diagnosis of human diseases by molecular genetic methods like PCR, RFLP, etc. at the Ahvaz Welfare Organization in Iran.

1994-1995 Responsible for Kits and reagent research and Development and production at the Pasteur Institute of Iran

1994-1998 Head of the Biochemistry Department at the Pasteur Institute of Iran.

1995-1996 Official delegate of the Director General of the Pasteur Institute in Hepatitis B Vaccine production.

1996-2003 Assistant /Associate Professor and Head of the Biochemistry Laboratory at the Pasteur Institute of Iran.

1998-2003 Director of Public Relations and International Affairs, Pasteur Institute of Iran.

2003-2005 Visiting scientist at Professor Cliff A. Lingwood’s laboratory Research Institute, in the Immunity Injury & Repair Program, Hospital for Sick Children Toronto, Ontario, Canada

2005-Present Researcher working with Professor Upton Allen, Division of Infectious Diseases chief, The Hospital for Sick Children, Toronto, Ontario, Canada

Teaching Experience

1994-2003 Practical and applied advanced Biochemistry and medical microbiology to PhD and MSc. students, Pasteur Institute of Iran.

Research Experience

1992-1993 Principal Investigator on the project of Detection of human cytomegalovirus (HCMV) in clinical specimens by a two-round polymerase chain reaction with nested primer oligonucleotides.

1992-1994 Principal Investigator on the project of Detection of Toxoplasma gondii and cytomegalovirus DNA in blood from transplant recipients by multiplex nested polymerase chain reaction

1994-1998 Principal Investigator on the project of Detection and Restriction Analysis of Viral DNA Sequences Persisting in Human Atherosclerotic Plaques by Using Nested polymerase Chain Reaction.

1995-1997 Co-investigator on a project of analysis of heavy metals in different kinds of milk by Atomic absorption spectroscopy in the Biochemistry Department of the Pasteur Institute of Iran

1996-1998 Co-investigator on the Project of Chemical synthesis of Oxytoxin, at the Pasteur Institute of Iran.

1996-1997 Working on designing recombinant vectors and expression in Escherichia coli of recombinant proteins composed of the Maltose Binding Protein from E.coli in which epitopes from the AIDS virus HIV were inserted into different positions and their immunological and antigenicity assay. in the Biotechnology Department, Pasteur Institute of Paris, France.

1997-1998 investigator on the project, Survey of prevalence of abortions due to CMV by using polymerase chain reaction (PCR) and comparison with Immunofluorescence (IF) in maternity clinic in Tehran, Virology Department, Pasteur Institute of Iran

1997-1998 Co-investigator on the project of investigation of Mercury level in blood and urine of Tehranian dentists. Pasteur Institute of Iran.

1997-1999 Investigator on the Project, production of Cyclosporine A, Pasteur Institute of Iran

1998-2001 Investigator on the project; Pilot Production of a Recombinant Human alpha Interferon in E.coli.

2003-06/2003

Evaluation of the interaction of exogenous DNA and Verotoxin1 B subunit-cro chimeric protein and Gb3 and its effect on DNA transfection into human tumoral cell line after exposing to Cro-VT chimeric protein by Luciferin/Luciferase method. Dr. Lingwood’s lab, Hospital for Sick Children, Toronto, Canada

2003-01/2005

Generation of a fusion protein of the A and B subunit genes of

the E.coli Verotoxin1 by gene splicing and overlap extension : initial

biological properties.

In Prof. Clifford A. Lingwood’s lab, Hospital for Sick Children, Toronto, Canada

2005-2010

Factor Predictive of EBV Lymphoproliferation Following organ Transplantation: Beyond Viral Load

2008-2012

Studying and working on prognosis and outcomes of EBV lymphoproliferation (PTLD) among organ transplant recipients and the role of gene expression profiling by using DNA microarrays. in Professor Upton Allen’s lab, Division of Infectious Diseases, The Hospital for Sick Children, Toronto, Canada

01/2009-present

Study and working on Epstein-Barr Virus in health and Disease in Children and Youth: A prospective Study of Markers of Virus-Host Interaction and Genetic Variation.

2010-2016

Primary EBV Infection in the Immunosuppressed Setting of Organ Transplantation: Determinants of the Outcomes of

Virus-Host Interaction.

2011-2015

Prevalence of 30-bp deletion variant of latent membrane protein-1 in pediatric transplant patients compared with patients with infectious mononucleosis.

2015-present

Genetic Diversity of Epstein - Barr virus in the Setting of Transplant

Relative to Non-Transplant Settings:

Memberships

Continuous member of the following Iranian Societies of:

Virology

Microbiology

Immunology and Allergy

Biotechnology

Biochemistry

1998-2002 Member of the National Committee of Biosafety and Ethics in Biotechnology; gathered, translated, edited from many international biosafety and ethic sources to be used as references in national wide.

Publications

1. Khodai Booran N., Bakaev, V Detection of human cytomegalovirus

(HCMV) in clinical specimens by a two-round polymerase chain reaction with

nested primer oligonucleotides. Ir. J. Med. Sci. Vol.20, No.3&4. (1995)

*2nd congress of Blood and Related Diseases, Tehran-Iran 21-23, (1996).

*Second international conference of Pakistan society for microbiology (1997).

2. Khodai Booran N., Bakaev, V., Assmar, M., and Hovanessian, A. Detection

of Toxoplasma gondii and cytomegalovirus DNA in blood from transplant

recipients by multiplex nested polymerase chain reaction Ir. Bio.

J., No.3. (1998)

*The International Congress of Blood Diseases, Montreal-Canada.. July, 2002,

*The fourth International congress of Immunology & Allergy, Iran, Isfahan,

13-15 May, (1998).

3. Khodai Booran N., Bakaev, V. Detection and Restriction Analysis of Viral

DNA Sequences Persisting in Human Atherosclerotic Plaques by Using Nested

Polymerase Chain Reaction. I. R.I. Med. J., Vol.15, No:3, Nov, (2001)

P.155-159.

*Pathology Congress of Professor Armin, Medicine faculty of Tehran (1996).

*Second Eastern Mediterranean Congress on Heart Health American

University of Beirut-Lebanon. May,2000,

4. Khodai Booran, N., Valery.V.Bakaev, Introduction to PCR Methodology;1996; BOOK. (In English).

*Khodai Booran, N., A practical manual for handling and transportation of clinical sample to microbiology laboratory. Book; 1993.

5. Saghiri, R., Tabatabaie, M., Khodai Booran, N., Borzani, M.V. A laboratory production of xylose isomerase. Second international conference of Pakistan Society for microbiology(1997).

6. Chinikar, S., Khodai Booran, N. Investigation of heavy metal levels in different kinds of Milk by using Atomic Absorption Method.

*The Fourth International Biochemistry Congress, Babol-Iran (1998).

7. Akbari, M.R., Khodai, N., Rechensky, V. Expression and Immunogenicity of

Escherichia coli heat-labile toxin B subunit: A candidate for oral vaccine

development. *The Fourth International congress of Immunology& Allergy,

Iran, Isfahan (1998).

8. Eliane Coëffier, Jean-Marie Clément, Valérie Cussac, Nasser

Khodaei-Boorane, Muguette Jehanno, Maurice Hofnung and Clude

Leclerc.Antigenicity and immunogenicity of the HIV-1 gp41 epitope ELDKWA

inserted into permissive sites of the MalE protein.Vaccine, Vol. 19 (7-8)

(2001) pp. 684 - 693.

9. Khodai Booran, N., Zarrin Pashneh, E., Identification of mycobacterium

tuberculosis strains by thin layer chromatography. The 14th Nationwide Congress on Tuberculosis, October 13-15, 1998, Kashan, Iran.

10. Khodai Booran, N., Bahri Najafi, M., Koloobandi, A, Biosynthesis of

Cyclosporine A from C.Inflatum.HPLC2002, 26th International Symposium on

High Performance Liquid Phase Separations and Relation Techniques. June 2-7,

2002, Montreal.CANADA.

11. Khodai Booran, Nasser, Tailor Chetankumar., Binnington Beth, Lingwood,

Clifford A. Generation of a fusion protein of the A and B subunit genes of

the E.coli Verotoxin1 by gene splicing and overlap extension : initial

biological properties. Presented in Sick Kids Scientific Retreat 2004.Toronto, Ontario, Canada.

12. N. Khodai Booran, J. Beyene, M. Forbes, S. Read and U. Allen. Abstract: Preliminary assessment of gene expression profiling using DNA microarray technology in patients at risk of Epstein-Barr virus (EBV) lymphoproliferation after transplantation. World Transplant Congress, July, 2006, Boston, MA, USA.

13. Upton Allen, Michelle Barton, Joseph Beyene, Pingzhao Hu, Nasser Khodai-Booran, Diane Hébert, Anne Dipchand, Vicky Ng, Melinda Solomon, David Grant, Annie Fecteau, Bo Ngan, Stanley Read, Maria Zielenska, Sheila Weitzman.

Gene expression using microarrays in transplant recipients at risk of EBV lymphoproliferation after organ transplantation: Preliminary proof-of-concept

Pediatric Transplantation; Volume 13, Issue 8, pages 990–998, December 2009.

14. U. Allen, P. Hu, M. Barton, J. Beyen, N. Khodai-Booran, D.Hebert, A. Dipchand, V. Ng, M. Solomon, D. Grant. O21 Differentially expressed host B cell-associated genes in high/intermediate versus low/undetectable viral load states in Epstein–Barr (EBV) virus infection after pediatricorgan transplantation.by:.International Journal of Antimicrobial Agents, Vol. 34 (July 2009), pp. S8-S8.

15. U Allen, P Hu, S Pereira, J Beyene, D Ho, N Khodai-Booran, T Nalpathamkalam, S Read (2012) Exploring genetic diversity of Epstein-Barr Virus (EBV) using whole genome next generation sequencing. International Congress on Oncogenic Herpesviruses and Associated Diseases, Philadelphia, Pennsylvania, USA, August 1-4, 2012.

16.N. Khodai-Booran, C.Tailor, B.Binnington E .Chan and C. A. Lingwood. An A-B Subunit Chimera of the E.Coli Verotoxin1 Provides a Receptor Probe and Potential Vaccine Approach. International Journal of Applied Biology and Pharmaceutical Technology Volume: 3: Issue-4: Oct-Dec -2012

17. Upton Allen, Pingzhau Hu, Sergio Pereira, Anne Dipchand, Vicky Ng, Diane Hebert, Melinda Solomon, Joseph Beyene, Tara Paton, Nasser Khodai-Booran, Thomas Nalpathamkalam and Stanley Read. Pilot Study of Genetic Diversity of Epstein-Barr Viral Genes Among Pediatric Solid Organ Transplant Recipients. American Transplant Congress, May 18 - 22, 2013 Seattle, WA.

18. N. Khodai-Booran, C.Tailor, B.Binnington E .Chan and C. A. Lingwood. An A-B Subunit Chimera of the E.Coli Verotoxin1 Provides a Receptor Probe and Potential Vaccine Approach .The 31st Annual Meeting of the European Society for Paediatric Infectious Diseases, May 28-June 1, 2013; Milan, Italy..

19. Upton Allen, Pingzhau Hu, Sergio Pereira, Nasser Khodai-Booran, Tara Paton. Genetic Diversity of Epstein - Barr virus in the Setting of Transplant

Relative to Non-Transplant Settings: Proof-of-Principle.

AMMI Canada - CACMID Annual Conference

VICTORIA, BC April 3 – 5, 2014

20.Correlates of illness severity in infectious mononucleosis.

Odame J1, Robinson J, Khodai-Booran N, Yeung S, Mazzulli T, Stephens D, Allen UD.

Can J Infect Dis Med Microbiol. 2014 Sep;25(5):277-80.

21. U. Allen, N. Khodai-Booran, T. Paton, S. Yeung, A. Dipchand, V. Ng, D. Hébert, M. Singh. Prevalence of 30-bp deletion variant of latent membrane protein-1 in pediatric transplant patients compared with patients with infectious mononucleosis.

8th International Congress of The International Pediatric Transplant Association (IPTA). March 28 – 31, 2015, at the Westin St., San Francisco, California.

22. U. Allen, N. Khodai-Booran et al. Division of Infectious Diseases1, the Transplant and Regenerative Medicine Centre2 and the Research Institute3, Hospital for Sick Children, University of Toronto

The genetic diversity of Epstein-Barr virus in the setting of transplantation relative to non-transplant settings: A feasibility study. 2016 Feb; 20 (1):124-9.

23. Marianna Abdulnoor, Nasser Khodai-Booran, PhD1, Tara Paton, PhD3, Upton Allen, MD, FIDSA1,2. Hospital for Sick Children, Toronto, ON, Canada, (3) Research Institute, Hospital for Sick Children, Toronto, ON, Canada. ID Week 2017TM, October 4-8, 2017 at the San Diego Convention Center, in San Diego, CA.

Genetic Diversity of Epstein - Barr virus Lytic Gene BZLF-1 among Patients with and without Post-transplant Lymphoproliferative Disorder.

24. Pietrzyk J 1, Khodai-Booran N 1, Casallo G 2, Allen U 1,2Genetic Diversity of the Epstein Barr Virus Nuclear Antigen 1 (EBNA-1) among Patients with Infectious Mononucleosis and Organ Transplant Recipients. The International Conference on EBV and KSHV, July 2018, Madison, Wisconsin, USA.

26.U. Allen, N. Khodai-Booran et al. Epstein-Barr Virus Genetic Diversity in Blood versus Saliva Samples from Patients with Infectious Mononucleosis. Presented at ID Week October 2018.

27.Genetic Diversity of Epstein–Barr Virus Lytic Gene BZLF-1 among Patients with and Without Post-transplant Lymphoproliferative Disorder. 2018. Upton Allen, Nasser Khodai-Booran, Tara Paton, Melinda Solomon

28.Phylogenetic Analysis of the Epstein Barr Virus Nuclear Antigen 1 and Associated Correlates among Canadian Transplant Patients. Pediatrics Research Day, May 29, 2019. The Hospital for Sick Children-Toronto, Canada.

M. Abdulnoor1, S. Isabel1, T. Paton1, N. Khodai-Booran1, T. Schechter1, P. Birk2, T. Blydt-Hansen3, B. Foster4, S. Mital1, V. Phan5, S. Urschel6, G. Casallo1, T. Papaz1, L. Hamiwka7, S. Min1, U. Allen1

1University of Toronto, Toronto, ON, CANADA, 2University of Manitoba, Winnipeg, MB, CANADA, 3University of British Columbia, Vancouver, BC, CANADA, 4McGill University, Montreal, QC, CANADA, 5University of Montreal, Montreal, QC, CANADA, 6University of Alberta, Edmonton, AB,

29. Epstein-Barr Virus Genetic Diversity: Preliminary Study of BZLF1 Variants among Transplant Patients in the CNTRP POSITIVE Study. Pediatrics Research Day, May 29, 2019. The Hospital for Sick Children-Toronto, Canada.

Mariana Abdulnoor1, Nasser Khodai-Booran1, Tal Schechter2, Tara Paton3, Guillermo Casallo3, Tanya Daljevic4, Sandar Min4, POSITIVE Study Investigators, Upton Allen1, 3.

Division of infectious disease, The Hospital for Sick Children 2. Division of Hematology/Oncology, the Hospital for Sick Children 3. Research Institute, the Hospital for Sick Children 4. Heart Centre, Division of Cardiology, the Hospital for Sick Children

30. Immunogenicity of a Quadrivalent Human Papillomavirus Vaccine in Pediatric Kidney and Liver Transplant Recipients (manuscript is ready for publish) 2021-2022

Taito Kitano MD, MPH a, Kevin L. Schwartz MD, MSc, FRCPC b,c,d,e, Mariana Abdulnoor BSc a, Hartley Garfield MD f, Nasser Khodai Booran PhD a, Yaron Avitzur MD g, Chia Wei Teoh MB, BCh, BAO, MRCPI, LRCSI h, Diane Hebert MD, FRCPC h, Upton Allen MBBS, MSc, FRCPCa

Languages:

Persian mother tongue

English fluent

French adequate

Turkish (Azeri) fluent

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