Dr. M. Rajasekar, Msc, M.Phil, PhD.

S/O A.Muthusamy

No.16, Ramadoss nagar, 2

nd

st, Aladi road

Virudhachalam – 606001

Cuddalore Dt, Tamil Nadu, India.

E.Mail: adppkq@r.postjobfree.com

Mobile: +91-801******* (WhatsApp), 861*******

ACADEMIC PROFILE:

Year of

Degree Branch Marks/OGPA % passing University/Board Dept. of Biochemistry and

Ph.D Biotechnology NA Biotechnology,

Awarded Annamalai University,

(on Aug Annamalai Nagar -608002

11

th

2017) Tamil Nadu, India.

CAS in Marine Biology,

M. Phil Marine 8.30 2012 Annamalai University,

Biotechnology Annamalai Nagar -608002

Tamil Nadu, India.

Dept. of Biochemistry and

M.sc Biotechnology 6.50 2009 Biotechnology

(integrated) Annamalai University

Annamalai Nagar -608002

Tamil Nadu, India

SUMMARY OF THE PH. D WORK:

Title: Evaluation of chemopreventive potential of Diosmin on DMBA induced oral carcinogenesis - An in vitro and in vivo approach. Oral cancer is a major cause of morbidity and mortality in developing countries. Despite have advances in chemotherapy for the cancer management. However, the survival rate has not yet been improved, because poor understating about molecular mechanisms of cancer. Understanding the molecular mechanism and oncogenic signaling events would help for the prevention and/or clinical treatment of oral cancer. Dietary nutrient has been received a lot of attention in the development of novel chemotherapeutic drug. Diosmin may be a naturally occurring flavonoid glycoside that can be isolated from varied plant sources may be a potent inhibitor that functions as a strong antioxidant and anti-inflammatory, free-radical scavenging activity. In the present study, we investigated the chemopreventive efficacy of Diosmin in DMBA induced hamster buccal pouch (HBP) carcinogenesis. Oral squamous cell carcinomas (OSCC) were induced in HBPs by painting with 0.5% of 7,12- dimethylbenz(a)anthracene (DMBA), thrice in a week for 16 weeks. We observed 100 % tumor incidence by the consequence of increased levels of lipid peroxidation and decreased antioxidant and detoxification enzymes. Furthermore, defective and up-normality of inflammatory, cell proliferative, apopototic and angiogenesis events in DMBA induced hamsters.

Oral administration of Diosmin significantly inhibits tumor development as evidenced by the possible mechanism of enhanced antioxidant, detoxification enzymes and decreased lipid peroxidation, phase I enzymes and glycoconjugates in DMBA induced hamsters. Moreover, Diosmin could modulate Bax/BcL-2 ratio and significantly increased p53 expression subsequently leads to apoptosis. Furthermore, Diosmin inhibits inflammation (IL-1, IL-6, IL-8, TNF-α, COX-2, CRP), cell proliferation and angiogenesis markers (PCNA, Cyclin D1, VEGF, HIF-1α) through inhibits transcriptional activation of NF-κB, JAK/STAT-3, PI3K/AKT signaling in DMBA induced animals. Additionally, we have investigated the anticancer effect of Diosmin was evaluated in Hep-2 cell lines. Diosmin preferentially inhibited cell proliferation and induced apoptosis in Hep-2 cells through enhanced ROS generation thus in loss mitochondrial membrane potential, increased oxidative DNA damage and modulate apoptotic markers (Bcl-2, Bax, caspases-9, caspases-3). Thus, findings suggested that Diosmin have potent anti-tumor activity against DMBA induced HBP carcinogenesis. Therefore, Diosmin could be used as a novel therapeutic agent for the medical treatment and/or prevention of cancer in the future.

Research Areas and Experiences

Cancer Biology, genetic variation studies in seabass (Lates calcarifer), Dietary antioxidants. Ph.D Dissertation Title:

Evaluation of chemopreventive potential of Diosmin on DMBA induced oral carcinogenesis An in vitro and in vivo approach

M.Phil (2010-2012)

Genetic variation studies in wild and captive populations of Seabass, Lates calcarifer using molecular markers

Research Assistant (RA) (2012-2015):

ICMR-Major research project

Annamalai University,

Annamalai Nagar - 608 002, India.

Project Title: Evaluation of Chemopreventive potential of Diosmin on DMBA induced oral carcinogenesis An in vitro and in vivo approach Technical exposure

Cell culture techniques

Subculture and maintaining cell lines

Determination of cell viability & cytotoxicity by MTT-assay

Measurement of ROS and MMP

Apoptotic morphological changes in fluorescent microscope using different probe staining

Single cell gel electrophoresis (Comet assay).

Molecular Biology

techniques DNA and RNA isolation

RT-PCR and qRT-PCR techniques

Immunoprecipitation

Western Blot analysis

Hematoxylin and Eosin staining

Histological

techniques Immunohistochemistry

Confocal and fluorescence microscopy handling

Isolation of cell Mitochondrial isolation

organelles

Cytosolic and nuclear fractionation

Biochemical

Enzyme assays using UV spectrophotometer and

ELISA

techniques reader

Animal handling Small animal handling (rat and mice) and maintenance

Drug administration (subcutaneous, intraperitoneal injection and oral gauage)

Animal sacrification and dissection

Work Experience:

1. Worked as guest lecturer in biotechnology department for one year from 18.6.2018 to 16.4.2019 in Post graduate Extension center, Villupuram, Thiruvalluvar University. Tamilnadu

2. Worked as Scientist in Karpagam Academy of Cancer Research Center, from 11.11.2019 to May 2020, (5 months) Coimbatore, Tamilnadu

RESEARCH PUBLICATION:

1. Muthusamy Rajasekar, Kathiresan Suresh, Kathiresan Sivakumar. Diosmin induce apoptosis through modulation of STAT-3 signaling in 7,12 dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis. Biomedicine & Pharmacotherapy. 2016; 83: 1064-1070. 2. Kathiresan Suresh, Muthusamy Rajasekar, Govindan Annamalai, Ramu Arun kumar. Scanning Electron Microscopy of Diosmin treated experimental animals on 7, 12-dimethylbenz (a) anthracene induced oral carcinogenesis in hamsters. International Journal of Advanced Research in Biological Sciences. 2015; 2 (1): 114–118.

3. Kathiresan Suresh, Muthusamy Rajasekar, Ramu Arun Kumar, Kathiresan Sivakumar. Protective Effect Of Diosmin On 7,12-Dimethylbenz (A) Anthracene

(Dmba) Induced Hamster Buccal Pouch Carcinogenesis. Life Science Archives. 2016; 2 (4): 601-609.

4. Arokia Vijayaanand Mariadoss, Suresh Kathiresan, Rajasekar Muthusamy, Sivakumar Kathiresan. Protective effects of [6]-paradol on histological lesions and immunohistochemical gene expression in DMBA induced hamster buccal pouch carcinogenesis. Asian Pac J Cancer Prev. 2013; 14(5):3123-9. 5. M.Rajasekar, M.Thangaraj, T.R.Bharath kumar, K.Muthazhagan Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers. Notulae Scientia Biologicae, 2012; 4(3):33-37. 6. J. Elizabeth Parveen, M. Thangaraj, T.T Ajith kumar, B.V. Bhimba M. Rajasekar. RAPD Probe on Genetic variation in Damselfishes (Family Pomacentridae) in Gulf of Mannar region. Asian Journal of Experimental Biological sciences. 2011; 2 (4): 687- 690.

7. Suresh Kathiresan, Arokia Vijayaanand Mariadoss, Rajasekar Muthusamy and Sivakumar Kathiresan. Protective Effects of [6]-Shogaol on Histological and Immunohistochemical Gene Expression in DMBA Induced Hamster Buccal Pouch Carcinogenesis. 2nd International Conference on Environment, Agriculture and Food Sciences (ICEAFS'2013) August 25-26, 2013 Kuala Lumpur (Malaysia). 8. S. Sivasakthi, P. Saranraj, M. Rajasekar. Optimization of Cellulase Production by Aspergillus Niger using paddy straw as substrate. International Journal of Advanced scientific and Technical Research. 2011; 1 (1): ISSN 2249-9954S PRESENTATIONS AND PARTICIPATIONS IN CONFERENCES:

Presented (Oral presentation) a paper during the 5 th

Annual Conference of the

Society for Mitochondrial Research and Medicine (SMRM-2015), held at Aravind Medical Research Foundation, Madurai, from 6 th

- 7

th

November

2015

Participated in four days “Workshop and Training on Sequence Analysis and Molecular Simulation” between 20

th

- 23

rd

January 2016 organized by the

Department of Biotechnology, Anna University, Chennai.

Attended the One-day Hands – on training on Flow Cytometer (HTFC-2015) organized by Centralized Instrumentation and Service Laboratory, Department of Physics, Annamalai University, Annamalai Nagar on 7 th

October 2015.

Attended UGC Sponsored RESEARCH METHODOLOGY WORKSHOP organized by the Department of Economics, Annamalai University, during 24 th

– 27

th

March 2014.

Attended National Conference on “Trends in Medical Biotechnology: Opportunities & Challenges (TMBT-2014)” organized by Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, India, March 7

th

& 8

th

, 2014.

Participated in national level technical symposium Genesis 2k5 conducted by Dept of Biotechnology Bharathidasan University on 14 th

and 15

th

of October

2005.

Participated in National conference on Diabetes and cancer during 15 th

& 16

th

Dec, 2007 in the department of Biochemistry and Biotechnology, Annamalai University.

Participated in International conference on Diabetes and mellitus and cancer during 14-16

th

Dec 2012 in the department of Biochemistry and Biotechnology, Annamalai University.

Presentation on a topic entitled Evolutionary Biology of cancer: An Overview in Biochemical Society – Research Colloquim Department of Biochemistry and Biotechnology, Annamalai Nagar, Chidambaram on 29

th

July 2015.

SOCIAL ACTIVITIES:

1. Got a certificate of appreciation from Tamil Nadu State AIDS Control Society & Tamil Nadu State Blood Transfusion Council for voluntary blood donation service to suffering patients on 22

nd

March 2012.

2. Got a certificate of appreciation from Rajah Muthiah Blood Donors Club, Rajah Muthiah Medical College and Hospital, Annamalai University, Chidambaram. 3. Got a certificate of appreciation in English Essay competition in National Science Day Celebration – 28

th

Feb 2014.

4. Got a certificate of appreciation and placed 4

th

place in university level Essay

Competition for Research Scholars on “Human Rights” organized by Department of Political Science & Public Administration, Annamalai University on 30

th

March, 2016.

5. Actively participated in National Service Scheme & Special Camping Program and got certificates.

SUMMARY

My ambition is to gain experience in novel areas of research in growth-oriented society where my skills and abilities will be utilized to the mutual benefits.

I acquired 5 years of research experience in the field of cancer biology.

I worked as a Research Assistant, in ICMR-Major research project during my doctoral research.

I have experience in grant writing, animal handling, cell culture, biochemical and molecular biology techniques.

I have ability to work with a team of scientists with diverse background, shares knowledge and expertise with lab members.

I am a self motivated and experienced academician who works well with minimum supervision.

DEMOGRAPHIC PROFILE

Date of Birth : 22

nd

July, 1987

Gender : Male

Father’s Name : A. Muthusamy

Mother’s Name M. Santhi

No: 16, Dr. Ramadoss nagar 2

nd

street,

Residential Address Aladi road, Virudhachalam-606001 Marital Status : Married

Nationality : Indian

Languages Known : English, Tamil (read, write and speak) Declaration

I hereby declare that the details furnished above are true to the best of my knowledge. Yours faithfully

M. Rajasekar

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