Japanese Postdoctoral Fellow

HANI AL-SHAREEF, B.D.S., PH.D.

Graduate School of Medicine, Osaka City University

Oral and Maxillofacial Surgery

Molecular Oncology and Therapeutics

*-*****-*-* **********, Abeno-ku, Ōsaka-shi, Ōsaka-fu 545-0051 Japan

******************@*****.***

****.**-*******@***.*****-**.**.**

Tel.: +818*-****-****, Skype: hani3008

ACADEMIC POSITIONS & JOB HISTORY

Postdoctoral Fellow, Japan Society for the Promotion of Science (JSPS) Nov 2019 – present

Combination of Oncolytic virus, immune checkpoint blockade and Semaphorin/Neuropilin/Plexin (SNP) Complexes blockade as a novel promising effective immunotherapeutic option in CAF and related refractory/relapsed cancer

Osaka City University, Graduate School of Medicine

Oral and Maxillofacial Surgery, Molecular Oncology and Therapeutics

Postdoctoral Researcher Oct 2017 – Nov 2019

Oncology (Immunotherapy, Virotherapy, Cell biology, Basic science, Apoptosis, Invadopodia)

Osaka City University, Graduate School of Medicine

Oral and Maxillofacial Surgery, Molecular Oncology and Therapeutics

Project Collaborator Jul 2018 – Oct 2019

Study of lymph node metastasis of tongue cancer using image recognition technology and pathological images using Deep Learning, molecular biological and data science studies,

Osaka University, Graduate School of Dentistry

Project Collaborator Aug 2016 – Oct 2017

Human Triple-Negative Breast Cancer (TNBC-Project), Data analysis for a Ph.D. student at

Osaka University and National Institute of Biomedical Innovation, Health and Nutrition

Teaching Assistant May 2014 – Mar 2016

Graduate School of Dentistry, Osaka University, Japan

Graduate Research Apr 2012 – Mar 2016

Oral cancer studies (Molecular/Cell/Cancer Biology/Metastasis/Oral and Maxillofacial Surgery)

Graduate School of Dentistry, Osaka University, Japan

Advanced Clinical Trainee for Foreign Dentists Apr 2012 – Mar 2014

The First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, Japan

Research Student Apr 2011 – Mar 2012

Graduate School of Dentistry, Osaka University, Japan

General Dentist Feb 2007 – Feb 2011

Al-Farabi Medical Center, Ministry of Health, Sana’a, Yemen

Intern Aug 2007 – Aug 2008

Oral Surgery and Oral Medicine, Conservative Dentistry, Prosthodontics, Preventive Dentistry

AL-Thawra Hospital, Ministry of Health, Sana’a, Yemen

SKILLS AND EXPERIENCE

Research Skills:

Immunotherapy procedures; T cells isolation, activation, differentiation, Cytokine release assays, Human blood samples handling, etc.

Animal experiments and handling, Knocked mice, (mice and rats)

Flow Cytometry, Intracellular and Cell Surface staining

Invadopodia Procedures

Immunofluorescence Procedures

Immunocytochemistry Procedures

Cell Culture Techniques

Assays; ELISA, FISH

Western Blotting Procedures

Gene editing, Transfection, Single cell cloning

Cell-based assay, Protein Production, Purification

DNA, RNA extraction etc.

Next Generation Sequencing (NGS)

Differential Gene Expression Analysis: Direct DNA, RNA Sequencing

Octet (kinetics, affinity, and antibody/protein quantitation),

AKTA (chromatography and protein purification)

Hybridoma technology and antibody production and cloning

Data handling and bioinformatics

R programming uses

Python programming uses

Basic bioinformatics uses

Basics in Deep Learning Specialization

On project of using deep learning idea to recognize the pathological images of primary and metastatic cancer with Python and PyTorch.

Statistical Analysis: Data mining; using Cancer databases of (TCGA), (GDSC), Geneontology, TargetMine,(GEO) datasets from NCBI for gene expression and microarrays, correlations, heat maps, hierarchical clustering, survival analysis, using R programming, Python, IBM SPSS, SAS, Excel, StatView, Sigma Plot, GraphPad Prism…etc.

Dental Expertise

Dentistry Oral Surgery Implantology

Orthodontics Periodontics Prosthodontics Prevention Cosmetic Dentistry Restorative Dentistry

Oral Medicine Oral Pathology Oral Radiology

Oral and Maxillofacial Surgery as an Assistant and Minor Surgeon

PUBLICATIONS

Kawamura Kohei, Lee Chonho, Yoshikawa Takashi, Hani Al-Shareef, Usami Yu, Toyosawa Satoru, Kogo Mikihiko, Tanaka Susumu, Hiraoka Shin-ichiro.“Prediction of cervical lymph node metastasis from immunostained specimens of tongue cancer using a multilayer perceptron neural network” International Journal of Cancer [submitted] IJC-21-2620

Kohei Kawamura, Shin-ichiro Hiraoka, Hani Al-Shareef, Chonho Lee, Takashi Yoshikawa, Yu Usami, Noriaki Tanaka, Susumu Tanaka, Mikihiko Kogo. “Study of lymph node metastasis of tongue cancer using image recognition technology and pathological images using Deep Learning “conference paper Japanese Society of Oral Oncology - J-Stage 2018, Japan Society for Head and Neck Cancer 2019

Hani Al-Shareef, Yudai Matsuoka, Mikihiko Kogo, and Hirokazu Nakahara. “Effects of decreased Rac activity and malignant state on oral squamous cell carcinoma in vitro” Accepted PONE-D-19-02913, Plos One 2019. Peri-submitted bioRxiv. Febraury 2019:540591. doi:10.1101/540591

Hani Al-Shareef, Yudai Matsuoka, Mikihiko Kogo, and Hirokazu Nakahara. “Inhibition of Rac Suppresses Protein Phosphatase 5 Activity, Inducing Hyper-Activation of c-Jun N-Terminal Kinase and Caspase-Dependent Apoptosis” [submitted] 2019 under revision

Kazuma Harada, Hani Al-Shareef, Yosuke Shogen, and Hirokazu Nakahara. “A case of dental surgery intervention for a hemophilia A patient” International journal of Oral Health Sciences 2019. DOI: 10.4103/ijohs.ijohs_36_19 * Equal order as the first author

Hani Al-Shareef, Shin-ichiro Hiraoka, Noriaki Tanaka, Yosuke Shogen, Doksa Lee, Sanam Bakhshishayan, Mikihiko Kogo. 2016. “Use of NRP1, A Novel Biomarker, along with VEGF-C, VEGFR-3, CCR7, and SEMA3E, to Predict Lymph Node Metastasis in Squamous Cell Carcinoma of the Tongue.” Oncology Reports 2016 Nov;36(5):2444-2454. doi: 10.3892/or.2016.5116. Epub 2016 Sep 21

Doksa Lee, Koichiro Wada, Yoshitaka Taniguchi, Hani Al-Shareef, Tomotake Masuda, Yu Usami, Tomonao Aikawa, Masaya Okura, Yoshinori Kamisaki, and Mikihiko Kogo. 2014. “Expression of Fatty Acid Binding Protein 4 is Involved in the Cell Growth of Oral Squamous Cell Carcinoma.” Oncology Reports 2014 Mar;31(3):1116-20. doi: 10.3892/or.2014.2975. Epub 2014 Jan 13

Ongoing projects and manuscripts in draft

Triple combination of oncolytic virus, immune checkpoint blockade and SNP complexes blockade as a novel promising effective immunotherapeutic option in refractory/relapsed cancer (JSPS project)

A review study, A characteristic comparison study for the mechanism and the therapeutic measures in novel SARS-CoV-2 virus and Oncolytic viruses

A review study, Management of patients with oral cancer during the covid-19 pandemic compared to Severe acute respiratory syndrome (SARS) pandemic

Therapeutic strategies options of the oncolytic herpes simplex virus vector, G47Δ in squamous cell carcinoma treatment in vivo and vitro models.

Combination Immunotherapy strategies with PD-1/PD-L1 axis blockade including CD4, and CD8 T Cells, CTLA-4, Anti-Semaphorin 4D_CD100 and Semaphorin 3E antibodies in vivo and vitro models.

Clinicopathological features and outcomes of vascular endothelial growth factors, semaphorins, and neuropilins in squamous cell carcinoma.

The outcome effect of knocking down NRP1, VEGF-C, VEGFR-3, CCR7, and SEMA3E on squamous cell carcinoma.

Study case of a cancer patient with Immune thrombocytopenic purpura (ITP) disorder along with surgical intervention

Invadopodia and Matrix Degradation regulations in oral, gastro, hepatic, and colorectal cancer cell lines a morphological cancer study.

Poster Presentation

Hani Al-Shareef, Shin-ichiro Hiraoka, Noriaki Tanaka, Mototomi Tokumiya, Chiaki Yamada, Masaaki Kimata, Sanam Bakhshishayan, Masaya Okura, Mikihiko Kogo. “Lymphangiogenesis Mechanism Predicts Correlations with Metastatic SCC and Prognosis.” 59th Congress of Japanese Society of Oral and Maxillofacial Surgeons, October 2014

AWARDS/RECOGNITION

FY2019 JSPS

Postdoctoral Fellowship for Research in Japan (P19415) Aug 2019

Top Student Award

Ph.D. degree with honor, and presented as the top student for Ph.D. Degree in Oral Science, Graduate School of Dentistry, Osaka University, 2016

Japanese Government Scholarship (MEXT Grant)

Ministry of Education, Culture, Sports, Science and Technology, Japan

Ph.D. Student at Graduate School of Dentistry, Osaka University, 2012 – 2016

Japanese Government Scholarship (MEXT Grant)

Ministry of Education, Culture, Sports, Science and Technology, Japan

Research Student at Graduate School of Dentistry, Osaka University, 2011 – 2012

EDUCATION

Osaka University, Japan Apr 2012 – Mar 2016

Ph.D. degree with honor in Oral Science Studies,

Graduate School of Dentistry, The First Department of Oral and Maxillofacial Surgery

Thesis Title: “Lymphangiogenesis Mechanism Predicts the Correlation between Metastatic Oral Squamous Cell Carcinoma and Prognosis.”

Sana'a University, Yemen Sep 2001 – Jul 2007

Bachelor degree in Dentistry, Dental Surgery (BDS)

Faculty of Dentistry

Taiz University, Yemen Sep 1999 – Jul 2001

Biology and Chemistry Courses, Faculty of Science, Chemistry/Biology

ACADEMIC CONFERENCES

Osaka International Cancer Center conferences and Symposiums, Osaka, Japan, 2016, 2017, 2018

iPS Cells for Regenerative Medicine, The 18th Takeda Science Foundation Symposium on Bioscience, Kyoto, Japan, January 2015

CiRA International Symposium, The 18th Takeda Science Foundation Symposium on Bioscience, Kyoto, Japan, January 2015

60th Congress of the Japanese Society of Oral and Maxillofacial Surgeons, Nagoya, Japan, October 2015

59th Congress of the Japanese Society of Oral and Maxillofacial Surgeons, Tokyo, Japan, October 2014

57th Congress of the Japanese Society of Oral and Maxillofacial Surgeons, Yokohama, Japan, October 2012

56th Congress of the Japanese Society of Oral and Maxillofacial Surgeons, Osaka, Japan, October 2011

ADDITONAL COURSES AND EXPERTIES

Peer reviewer Journal of Oral and Maxillofacial Surgery, Medicine and Pathology, Elsevier, May 2016 – present

Statistics in Medicine, Online Course, Stanford University, USA, August 2013

English Language Diploma, Seeds Center Sana’a, Yemen, April 2009 – March 2010

Creation and Management of Small and Micro Business and Marketing Course, French Embassy and Ministry of Youth, Sana’a, Yemen, June 2008 – August 2009

TUT Dental Implants System Course, Sana’a, Yemen, May 2007

Basic Orthodontics Course, Sana’a, Yemen, 2007

Basic Life Support (BLS) Course, Mosul University, Iraq, September 2002

Computer Maintenance Technique Course, Mosul, Iraq, 2002

MEMBERSHIPS AND VOLUNTARY ACTIVITIES

Member: Japanese Society of Oral and Maxillofacial Surgeons (JSOMS), August 2012 – present

Leader: Osaka University International Students Association (OUISA), April 2011 – 2016

Member: The International Youth Exchange Organization of Japan (IYEO), Japan, April 2011 – present

Member: SWYAA, Japan, The Ship for World Youth Alumni Association (SWYAA) supported by United Nations University and Government of Japan, UN, Education, Environment, Volunteering, Cross Culture Understanding, January 2010 – present

Volunteer: National Cancer Control Foundation, Yemen, September 2009 – September 2010

Member: Japanese-Yemeni Friendship Association (JYFA), Yemen, Japan, May 2008 – present

Member: The National Union for Development the Poorest (Marginalized), Yemen, September 2008 – present

Member: The Yemeni Medical Charitable Association, Health Care, Social Services, Education, Yemen, September 2007 – present

Member: The General Union of Yemeni Students, Iraq, Yemen, 2001 – 2007

REFERENCES

Reference 1:

Professor Hirokazu Nakahara, DDS, Ph.D.

Osaka City University, Graduate School of Medicine

Head of Department of Oral and Maxillofacial Surgery

********.********@***.*****-**.**.**

Tel: +81 6 6645 2781

Reference 2:

Associate professor Masakazu Yashiro, MD., Ph.D.

Osaka City University, Graduate School of Medicine

Head of Department of Molecular Oncology and Therapeutics

Surgical Oncology, Cancer Center of Translational Research

********@***.*****-**.**.**

Tel: +81 6 6645 3936

Reference 3:

Mustafa Sami, Ph.D.

Scientist and data analysist at

RIKEN Center for Developmental Biology

****.****@*****.***

*******@***.*****.**

TEL +81-78-261-****

Contact this candidate