Contact

Tel: 281-***-****

E-mail : ado057@r.postjobfree.com

PRASANTI YERRAMILLI

Address

**** ********** *****, *********, ** 95014

Professional Summary

Detail oriented enthusiastic biologist with Master of Science in microbiology

6 years of research experience in the field of molecular biology

Seeking to contribute to the advancement of science by performing accurate research and complying with all laboratory safety protocol and standards

Strong leadership skills and lab management experience

Have a deep desire to learn new technologies in the field of cell and molecular biology

Key skills and attributes

Fast learner of new processes

Love to work on new challenging issues

Proven ability to improve process efficiency of lab operations

Mentor and train new employees

Great attention to detail in lab work and documentation

Thorough knowledge of procurement and inventory management

Highly dependable with accuracy and quality of work delivered on time

Comprehensive knowledge of Microsoft Word, Office, Excel and PowerPoint

Designing of primers and generation of Isogenic gene deletion mutants

PCR techniques

Proficient using BD Rhapsody Single cell analysis system.

Generating libraries for whole transcriptome Analysis, AbSeq analysis, and Targeted mRNA from the BD Rhapsody Single cell capture beads.

Microbial techniques

Working knowledge of confocal microscopy

Protein purification

Immunochemistry, ELISA assays

Western Blotting and Coomassie staining for Protein Analysis

Basic Cell culture techniques

DNA/RNA isolation from various sample types, using both manual and automated processes

Purification and QC of nucleic acids using Nanodrop, Drop-sense spectrophotometry, Pico green, QuantIT, QubIT, 2100 Agilent Bioanalyzer system, Agilent 4200Tapestation and E-gel electrophoresis procedures

RNA isolation, cDNA synthesis, Library prep and sequencing

RT-qPCR

Proficient in using Beckman Coulter Biomek Automated liquid handlers

Programmed and used Hamilton Automated liquid handlers

Used Covaris S2 system for shearing DNA samples

Loop mediated isothermal amplification PCR

Working knowledge of flow cytometry and Fluorescence activated cell sorting (FACS)

Illumina PCR-free Indexed Paired-End Library construction

Sanger Sequencing

CoVID-19 Sequencing on the GridIon X5 Oxford Nanopore platform.

SNP array genotyping using Fluidigm and Illumina Infinium platforms

Used Illumina BaseSpace, Seven Bridges software and BD Data View to perform the data analysis for whole transcriptome Analysis, AbSeq analysis, and Targeted mRNA from the BD Rhapsody Single cell capture beads.

Proficient in High Throughput Next generation whole genome DNA sequencing (HiseqX, Hiseq 2000, Hiseq2500, Miseq, Nextseq and Novaseq)

4 years of experience in a fast-paced Research lab at HGSC, Baylor College of Medicine and in the Clinical lab at HGSC, Baylor College of Medicine

2+ years of experience as Research Assistant at the Center for Molecular and Translational Human Infectious Diseases Research, HMRI

6 months at BD Biosciences as a Scientist II, In the Single cell Multiomics Product development team

Scientist II, Single cell Multiomics, Product Development Team. BD Biosciences. San Jose, California [ June 2021 to current)

Roles and Responsibilities:

Developed Standalone assays for Whole Transcript Analysis, Targeted mRNA analysis, Sample tagging(for multiplexing)and Antibody sequencing for Single cell Multiomics Technology at BD Biosciences.

Evaluated the cell capture efficiencies between 2 bead types.

Improve the sequencing efficiency of the WTA libraries,Targeted libraries and Abseq libraries on the Illumina flow cell.

Tested and improved the robustness of the raw material(custom cell capture beads) to improve the reliability and reproducibility of the ScM assays.

Performed Sequencing data Analysis on BaseSpace and Seven Bridges to validate the sensitivity and reliability of the standalone WTA, Abseq and Targeted mRNA assays.

Lab Manager HT-LAMP Rice360 Institute for Global Health, Rice University, Houston TX 77030 [November,2020 to February,2021]

Roles and Responsibilities:

Developed and designed high throughput testing protocol for LAMP-PCR COVID-19 testing.

Wrote Protocols to be implemented for Testing students for Spring Move-in day in January 2021

Designed Covid-19 testing protocol for low resource setting.

Automated the high-throughput testing on the Hamilton liquid handlers.

Optimized the sample lysis buffer for better testing outcome.

Increased the testing efficiency by 47%.

Designed the sample collection site layout for efficient and safe processing of students during Spring Move-in day.

Developed and implemented a sample tracking and barcoding system to ensure the safety and accuracy of sample transfer through the pipeline.

Scheduled and managed daily work for the lab personnel.

Hired and trained new lab personnel.

Managed the lab inventory and procurement of all consumables and reagents to keep the lab running smoothly.

Presented testing data on a weekly basis.

Research assistant, Houston Methodist Research Institute [June,2019 to May, 2021]

Center for Molecular and Translational Human Infectious Disease Research.

Roles and Responsibilities:

Sequencing CoVID-19 samples using Illumina Novaseq Platform

Sequencing CoVID-19 samples using Grid Ion Oxford Nanopore technology

Viral RNA extraction from the CoVID Positive clinical samples

cDNA synthesis, library prep( Manual and automated)and loading on the sequencing platforms.

Inventory management for reagents and consumables, as necessary to ensure smooth operation of the lab.

Collecting the positive CoVID samples using the hospital’s tube tracking system.

Generation of Group A and Group B Streptococcus isogenic gene deletion mutants

Confirmed the gene deletion mutants with PCR validation and Sanger Sequencing

Processed the Input and output libraries to isolate genomic DNA and helped prepare Tradis libraries for sequencing on Nextseq platforms to compare the gene expression profiles in the mutants

Analyzed the quality and concentration of the libraries using QubiT, QuantIT, Nanodrop, gel electrophoresis and Agilent Bioanalyzer.

Diluted, denatured, and loaded Tradis libraries on Illumina Nextseq 550

Enzymatic DNA extraction and Illumina Nextera XT DNA library preparation for WGS of bacterial samples.

Isolated RNA from the isogenic mutants and performed the RT-qPCR to quantify the changes in gene expression levels in mutants.

Performed growth curve assays for GAS and GBS using BioTek Synergy2 plate reader.

Compared the biofilm formation in different GAS and GBS mutants using BioTek Synergy2 plate reader.

Performed CAMP and CAMP like tests on GBS mutants with weaker hemolysis to check if they restored a strong hemolytic phenotype in the presence of WT strains/ Staphylococcus Aureus.

Performed Protease activity assay for GAS and GBS strains

Helped with the study of growth of GBS and GAS in Saliva, blood and plasma.

Performed the E-test for multiple antibiotics on selected MGAS strains. And confirmed the Etest with liquid MIC assay different antibiotics.

Compared the NAdase and SLO activity of different GAS mutants using Western Blotting and Coomassie staining assay.

Performed ELISA test to get a quantitative measurement of the binding capacity of the pooled human Ig to the MGAS antigens.

Performed SLO Activity Assay for MGAS strains.

Research assistant, Baylor College of Medicine Human Genome Sequencing Center, CLIA Lab[October 2015 to June 2019]

Worked in 4 labs in the HGSC-CLIA. Nucleic acid lab, Sample intake lab, SNP array lab, and Sequencing lab.

HGSC-Nucleic acid Lab

Roles and responsibilities

●Isolated and purified high molecular weight genomic DNA and RNA from whole blood, buffy coat, saliva, tissue, and FFPE samples, and dried blood on Whatman paper using various isolation kits

●Used the LIMS system for efficient sample intake, sample tracking and sample handover to the library group after the initial QC using the NanoDrop, Dropsense or Pico green procedures

●Troubleshooting during the isolation process.

●Updated data into LIMS system for NA lab

●Validated the SOPs according to the CLIA requirements

●Ordered reagents and lab supplies for a steady uninterrupted functioning of the lab

●Organized the lab to get CAP accredited and CLIA certified

●Helped in the installation and validation of Chemagic-360 robot for automated nucleic acid isolation

●Develop and write SOPs for the different nucleic acid isolation processes

●Helped implement the Exemplar LIMS system for NA lab

Sample Intake Lab

Roles and responsibilities

●Received DNA and RNA samples in LIMS from different collaborating research labs and clinics

●Performed a thorough quality testing using PICO green assay, 2100 Agilent Bioanalyzer system, NanoDrop, Dropsense Spectrophotometry, and E-gel electrophoresis. And delivering accurate QC data within the turnaround time.

●Used Biomeck robots for efficient sample QC and aliquoting

●Worked on Gen2 LIMS system and Exemplar LIMS system to track and keep account of all the samples and data.

●Communicated with different collaborators to ensure proper sample shipment and procurement

SNP Array Lab

Roles and responsibilities

●Processed hundreds of Clinical and research samples every week for genotyping using Fluidigm Platform and Illumina Infinium platform.

●Prepared samples for Fluidigm 96.96 dynamic array, loaded on the IFC, read the chip on the Biomark HD system, and completed the genotyping analysis on Fluidigm software.

●Reported accurate data using Gen2 and Exemplar LIMS system within the scheduled time frame

●Used Gen2 and Exemplar LIMS system to track samples and data.

Sequencing Lab

Roles and Responsibilities:

●Conduct experiments daily on Next Generation Sequencing Platforms specifically

●Illumina Hiseq X, Illumina Hiseq 2000, Illumina Hiseq 2500, Illumina Miseq and Illumina NovaSeq Sequencing platforms.

●Thorough knowledge of the working protocol on these platforms. Have diluted and denatured libraries, used the cBot for cluster generation and loaded the flowcells on the above-mentioned platforms. Have done troubleshooting and resolved issues when required. Called technical support to resolve bigger issues. Have performed quality–control analysis on sequencing runs to ensure there are no errors, and the QC parameters are met. If required, acted upon prompt remediation of the issues to ensure the QC metrics are met. Have also performed final quality control analysis at the end of the runs ensuring successful data transfer for downstream analysis. Worked daily on the run data spreadsheets to make sure the data was accurate and updated for the Analysis Team.

●Managed work queue and helped assign Daily schedule to the staff in the lab and implemented day to day administrative tasks

●Supervised and trained new employees in the lab. Helped staff with technical issues daily.

●Maintained through records of laboratory experiments, results, and instrument maintenance.

●Prepared graphs and data in Microsoft Excel to communicate results.

●Demonstrated strict adherence to quality assurance policies and practices as outlined in the SOPs. Helped in updating the departmental SOPs, as necessary.

●Helped draft and finalize the SOPs for Novaseq and Hiseq 2000 platforms.

●Helped with generating the monthly cost reports.

●Used Gen2 LIMS and Exemplar LIMS system to receive and track samples during the sequencing run.

●Meticulously maintained an inventory of all the reagents and consumables in the lab. Ordered reagents and consumables, as necessary to ensure smooth operation of the lab.

●Ensured that sample libraries are stored at the appropriate temperatures and maintained logs so that library refrigerators/freezers are functioning properly.

●Assisted with maintenance of equipment and troubleshooting. Helped with proper handling, use, and disposal of biological/chemical waste and materials.

●Documented updates to safety practices and monitored safety equipment in work areas. Reported incidents of neglect or non-compliance immediately to supervisors for action.

●Enforced and maintained a clean work area to ensure a safe working environment.

●Helped write the SOP in CLIA lab for the Novaseq platform.

●Helped develop the Exemplar LIMS system for CLIA lab.

Ivaturi Consulting Inc. [July 2012 to September 2015]

●Worked as an administrative assistant for 3 years for a small IT Consulting firm.

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●Documented project deliverables discussed between the consulting company and business clients

●Prepared position papers with clients with appropriate detail of software services that were agreed between various parties

●Maintained detailed transactional data about accounting transactions in sales, payroll, and cash management applications

●Helped management with tax filing and reports

Education

●Master of Science in Microbiology

Orissa University of Agriculture and Technology, Bhubaneswar, India

Graduated at top of the class with distinction (Valedictorian) 1998

7.95/10 GPA

Course work: Principles in microbiology, microbial techniques, microbial physiology and biochemistry, microbial genetics and biotechnology, principles of immunology and serology, care and management of lab animals, general virology, clinical microbiology, food microbiology and computer applications.

Dissertation: Biosorption of Cu, Zn, and Cd using waste biomass.

Worked for dissertation under the guidance of Dr. G. Roy Chaudhury, Ph. D., in the Metallurgical Department at Regional Research Laboratory, Bhubaneswar, India

●Bachelor of Science in Botany (Majors), Zoology and Chemistry (Minors)

Utkal University, Bhubaneswar, India

Graduated top of the class with distinction

Awarded Miss Botany 1995

Activities and Interests

Research about health and nutrition.

Coached middle and high school students for various science competitions.

Master of Arts degree in Odissi Dance from Akhil Bharatiya Gandharva Mahavidyalaya Mandal Pune, India.

I studied the Indian classical dance, “Odissi” for 20 years. Performed in various cities in India and US.

Love to entertain and cook for friends and family.

Passionate gardener.

Great interest in traveling around the world and learning about new cultures

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