PROFILE

I tremendously enjoyed the tenure of my master and Ph.D. degrees I completed my laboratory experience both at university and during my internships, which allowed me to gain insight into basic research and to acquire useful technical and analytical skills. I am now well acquainted with many common molecular and cellular biology methods such as western blot, FACS, microscopy, cell culture, etc.. In addition, I acquired expertise in niche techniques such as the CRISPR Cas9 and isolation of stable clones. During Internship isolation of endothelial cells from veins of umbilical cords, angiogenesis assays and live-cell fluorescent imaging was studied. I also have some experience with handling mice, which I gained whilst working on a project involving immunohistology staining of decellularized tissue. Finally, my laboratory experiences taught me dedication, patience, and careful record-keeping, which I believe are essential in order to achieve success.

The projects in Pharma industry is particularly appealing to me because it's closely related to my work. Finally, working in pharma projects would not only enable me to apply my technical skills, which largely correspond to industry requirements but also to learn new techniques that I will be able to use in the future. I am looking for a position in an industry where I can apply my skills and progress along with the organization.

Technical Skills

Cell isolation, Cell Culture, Yeast Culture

Angiogenesis, FACS, Flow cytometry, Live-cell fluorescent imaging, Immunohistology staining, Microtome sectioning Lyophilization

Protein purification (e.g. immunoprecipitation, affinity purification)

Western Blotting

Polyacrylamide gel electrophoresis (1D and 2D, denaturing and native)

Basic biochemical assays

Microsoft Office and computer skills: (Word, PowerPoint, Excel and Outlook), Statistical data analysis, fundamentals of C programming, GraphPad, CLC genomics workbench

Multi-lingual: Fluent in English and Hindi, Marathi

PROJECT COMPLETED

EDUCATION

Ph.D. from University of Strathclyde, Glasgow 2016-2021

Diploma in Intellectual property law, Bharati Vidyapeeth, Pune 2011-2012

Masters in Biochemistry, University of Pune 2008 – 2010

Bachelors in Chemistry, University of Mumbai 2004-2007

WORK EXPERIENCE

Ph.D. at University of Strathclyde Glasgow, UK: Oct2016-Oct2021

oSilencing IKKα by using the CRISPR/Cas9 system to interrogate NF-kB non-canonical pathway in cellular cancer models. Thesis submission in progress.

Internship at the Department of Molecular Medicine and Surgery, CMM, Karolinska, Solna, Sweden: Sep2015-Dec2015

oProject: ‘Investigation of cytomegalovirus induced endothelial IL-11 production’.

oExperience in using various methods, Isolation of primary cells from human tissue such as veins of umbilical cords, angiogenesis assay, scratch assay, live-cell imaging, FACS assay, and ELISA.

Project trainee at the Laboratory for Transplantation Biology, Gothenburg, Sweden: Jan 2015-April 2015

oProject: ‘Excisional full-thickness skin wound healing by tissue-engineered graft prepared from porcine intestine in a blab/c immunocompetent mouse model.’ In this project we were interested in creating a dermal substitute with similar cellular organization to that of normal skin. Highly porous, decellularized ECM sheets were fabricated from porcine intestine via homogenization, centrifugation, chemical and enzymatic treatments, molding, and freeze-drying. At the early stage, the ECM sheet efficiently absorbed wound exudates and tightly attached to the wound surfaces in few mice. After four weeks of implantation, the wound was completely closed, epidemic cells were well arranged, and the bilayer structure of the epidermis and dermis was restored in some cases. Moreover, hair follicles and microvessels were newly formed in the ECM sheet-implanted wounds. Still my colleagues are working on this project.

Master’s Thesis project at Fergusson College Biochemistry Department, University of Pune: April 2010- Nov 2010.

oProject 1: ‘Inhibition and characterization of bacterial blight of Pomegranate caused by Xanthomonas axonopodis pv. Punicae’ Xanthomonas axonopodis pv. Punicae causes a lot of damage to the pomegranate crop and fruit which was real problem for the farmers all over India. Isolation of bacterial blight from the surface of pomegranate by using streak plate spread plate and pour plate microbial techniques. Successfully isolated bacterial blight was treated with Carica papaya and vitex negundo various concentrations of methanolic leaf extracts to find the cheap and crude way to inhibit its spread. vitex negundo plant extract shows inhibitory effects after 3 to 5 days of incubation.

oProject 2: ‘Comparison of the nutritional value of non-conventional leafy vegetables with conventional leafy vegetables’ Moringa oleifera and Cicer arietnum plants are widely distributed all over the world. Both bear large foliage most of it is considered as waste and is thrown away. From the results obtained from our study, it can be concluded that these samples are good nutritional supplements that can be incorporated into our diet. The samples contained higher concentration of protein and carbohydrate which is very important from nutritional point of view. The samples also showed presence of essential and semi-essential amino acids such as methionine, arginine, and tyrosine which enhances nutritive value of these leaves. The samples were also found to contain high anti-oxidant activities as it showed high phenolic content, good reducing and h2o2 scavenging activities. This feature of the leaves proved them to be more nutritive sources that provide protection against several diseases as good amounts natural anti-oxidants are present. The samples also contained high amounts of vitamin C and vitamin B2 as compared to other conventional leafy vegetables. The anti-nutritional factors like lectin showed very low activity in both the samples that make them more suitable for consumption. Drumstick pods are more widely used for consumption though it exhibits high lectin activity. Thus, the use of leaves should be preferred over pods. Various other anti-nutritional factors present in conventional leafy vegetables are not found in leaves of drumstick and chickpea. Thus, they can act as better supplements than spinach. Drumstick and chickpea are cost-effective nutritional sources. As a whole, many non-conventional food plants are at par with conventional ones but need further investigation.

PRIME SKILLS

Project Management

oStrategic and operational planning, executing, reporting, data collection and analysis

oManaged multiple projects, a track record of independent project management

oParticipated in concept and process development, design and optimization of SOPs/ protocols

oKnowledge & experience of GMP/GLP

oOffered mentorship and coaching to junior team members

Molecular Life Sciences and Clinical Skills

oMolecular biology, CRISPR/Cas9, Cell Biology techniques (inclusive of DNA/RNA extractions, PCR, plasmid construction/ cloning, Gibson Assembly, transgenic cell line development, protein expression/ modification, genome editing)

oAseptic cell culture techniques, Cell cycle and Apoptosis Assay On FACS

oAntibodies-based/ Immunoassays, enzyme, and cell-based assays, ELISA,

Western blots, Biological database handling

oMolecular Biology and Bioinformatics tools and software

oImmunofluorescence staining and slide preparation, Fluorescence microscopy, FACS/ Flow cytometry, DIVA and FlowJo

oScientific literature search (e.g. PubMed, EndNote), review and analysis

oMedical writing for Clinical case studies, biologics/ drug/ protein therapies, and medical devices

oClinical trials data analysis

oPreclinical / non-clinical research in life sciences

Strong oral and written communication skills: presentation with internal and external stakeholders, writing scientific reports and summaries

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