Application for the post of Scientist in R&D

Dr. S. SATHEESH KUMAR

N0-9,1st street, Seemathamman nagar

Madhuravoyal,

Chennai-600095

Mobile:+91-994*******/877-***-****

Email: admwhl@r.postjobfree.com

OBJECTIVE :

To take up a challenging carrier grows with honesty, loyalty, good relationship and best performance, and translate my experience, knowledge, skills and abilities into value for an organization.

Work Experience

Presently working as Consultant Microbiologist in SRL DIAGNOSTICS, CHENNAI. Roles and responsiblities is that VIRAL RNA EXTRACTION, PCR MASTERMIX PREPARATION, RESULT ANALYSIS, EQAS/QA MONITORING, ICMR REPORT FOLLOUP AND SOP PREPARATION.

Work responsibilities Aarupadai Veedu Medical college and Hospital:

Worked as Research Scientist in Aarupdai Veedu medical college and Hospital, Puducherry, major role was to establishing nCOVID 19 infection detection and also dealt with research work on dengue virus, human papiloma virus, HIV and influenza virus screening.

Work responsibilities at ICMR-NIE:

worked as Project Scientist –B from December 2013 to January 2020 under the project entitled ‘ ̏National Hospital Based Rotavirus Surveillance and vaccine Impact evaluation’ at National Institute of Epidemiology, Indian Council of Medical Research, Govt.of.India, Chennai.

Microbiology and Molecular biology based analysis of human pathogens from body fluids

Rotavirus adaptation in cell lines

Western blot analysis of rotavirus from children stools

Routine field visit to public health centre for confirmation of rotavaccination status

Screening of Rotavirus from childrens stools by ELISA

Detection of Rotavirus and Norovirus Genotype from children stools by reverse transcriptional PCR, real time PCR and sequence based analysis

Screening of dengue and influenza virus by realtime PCR

Case reporting form data analysis.

Guiding of M.Sc, B.Tech and M.Tech biotechnology students project

Stool aiquation and inventory maintenance

Review of NRSN project activity at Institute of Child Health & Hospital for Children (ICH&HC), Egmore, Chennai once in a week.

NRSN QC/QA concordance and proficiency test screening

Preparation of Laboratory SOPs and project reports

INSTRUMENTS EXPERTISE :

ABI sequencer-3500, Bio Rad T-100 PCR, ABI 7500 and Rotor gene 6000-real time PCR and Beckman flow cyto metry

BIO-RAD immunowash 1575 and Imark microplate reader

HPLC -Shimadzu

Beckman ultra centrifuge and GE healthcare tangential flow virus concentration filtration apparatus

Bio safety cabinet class II- and III Bioklenz and Laminar flow- Clean air system

Autoclave-JSR and Incubator and humidity chamber

BIO- RAD U.V.Visible spectrophotometer

Biomerieux-BACT/ALERT-3D and AQUALAB 4TE-Water activity meter

Digital refractometer and Erose viscometer and ADVIA centeur-Siemens

EXPERIENCE IN MEDICAL DIAGNOSTICS AS PRODUCT SPECIALIST :

Marketing and Promotion of molecular diagnostic kits and microbiology reagents and kits such as Realtime PCR-Gene based detection of human pathogens like HIV, HBV, HCV, HSV, Chikungunya, Dengue, H1N1, TB and Influenza virus.

EXPERIENCE IN MOLECULAR DIAGNOSTICS LABORATORY :

1.Senior Molecular Biologist from September 2007 to January 2008.

Organization : Neon Healthcare and Research Institute, Kolkatta.

Division : Molecular Diagnostics.

ROLES AND RESPONSIBILITIES.

Screening of HIV,MTB,HBV,HCV,HSV,CMV, Chikungunya, Dengue, BCR-ABL Chromosome fusion,Genotype using rotor gene-6000-Real-time PCR.

2.Real-time PCR and Flow cytometry analyst from March 2005 to August 2007.

Organizatiosn: Isotope Diagnostic centre, Chennai.

Division : Molecular Diagnostics.

ROLES AND RESPONSIBILITIES.

Screening of HIV,MTB,HBV,HCV,HSV,CMV, Chikungunya, Dengue,,Genotype using rotor gene-3000-Real-time PCR.

All Microbiological test and serological test analysis.

EXPERIENCE IN ACADEMIC INSTITUTION:

1. Lecturer in Microbiology Department From July 2004 to Sep 2006

Organisation : Pachaiyappas collehe, Chennai

Division: Microbiology and Molecular Biology

EXPERIENCE IN FOOD ANALYSIS :

Identification of microorganisms from sea food

Enumeration of microorganisms from sea food

Pathogen testing from sea food

Physico- chemical analysis of environmental samples

RESEARCH EXPERIENCE AND EXPERTISE :

10.3 years of research experience in the area of molecular virology (White spot syndrome virus, Rotavirus, Norovirus, Dengue and Influenza virus), in-situ DNA hybridisations, Immunology, histopathology, sediment physico chemical properties analysis, microbiology, cloning and expression, anti viral gene therapy, gene expression analysis, molecular identification of bacteria, fungi and actinomycetes. Bacteria Metagenomic library construction and viral metagenomic library construction. White spot syndrome virus transmission, viability and infectivity.

1.Seniour Research fellow from August 2010 to July 2013, in a project entitled ‘Transmission of White spot syndrome virus in Brackishwater ecosystems’.

Organization : Central Institute of Brackishwater Aquaculture (Indian council of Agricultural Research) Govt of India.

Division : Micro Biology.

2.Juniour Research fellow from December 2009 to July 2010, in a project entitled ‘Development of Antiviral gene therapy against shrimp viral pathogens’.

Organization : Central Institute of Brackishwater Aquaculture (Indian council of Agricultural Research) Govt of India.

Division : Molecular Biology.

Project Handled in CIBA :

Departmentof Biotechnology (DBT, Govt. Of India) funded project entitled’ Development of Antiviral gene therapy and virus transmission’.

RESPONSIBILITIES AND NATURE OF WORK :

Development of monoclonal antibodies against vibrio spp

Cell lines maintenance and White spot syndrome virus adaptation in mosquito cell line

Whole genome Amplification of environmental samples and cloning in to vector

Microbes were screened with special reference to antagonostic activity/ useful metabolites

Cloning of VP28 & RR1 gene in to pLitmus vector

dsRNA production by in Vitro synthesis

Virus purification using Ultra centrifuge

In Situ DNA hybridization for virus infection confirmation in shrimp.

Histopathology for shrimp disease diagnosis using Hematoxylin & Eosin stain.

Ph.D. programme:

My thesis work was carried out in ICAR-Central Institute of Brackishwater Aquaculture, Govt.of.India, Chennai. My Doctoral Research work on transmission and viability of White Spot Syndrome Virus (WSSV) in brackishwater ecosystems. Virus concentration from water and sediment method was developed and WSSV was isolated from large water samples by tangential flow ultra filtration technique. WSSV infection in clam gills (Meretrix meretrix) was confirmed and infection was first time proved by insitu DNA hybridizations. Viability and infectivity of WSSV has been analysed in host free virion water, sediment, microalgae and clam. Evaluated WSSV viability relation with sediment physicochemical properties.

M. Sc Dissertation Work:-

The dissertation submitted in the name of“identification of pathogenic micro organisms and antibiotic susceptibility pattern from catheter related infection in ICU Patient Done at Dept., of Microbiology, Ramachandra Hospital & Research Institute.

Academic Qualifications:-

Name and address of Institute/College

University Board

Degree

Specialization

Percentage

Year of Passing

ICAR-Central Institute of Brackish water Aquaculture

(CIBA),Chennai.

University of Madras

Ph.D

Gene Technology, Microbiology and Pathology

Feb 2020

J.J.College of Arts and Science Pudukkottai.

Bharathidasan University

M.Sc

Gene Technology

76.3

2004

Pachaiyappas college, Chennai.

University of Madras

B.Sc

Micro Biology

58.1

2001

Training work shop attended:- Training and worlshop on detection and genotyping of Noroviruses by rt-PCR and sequence analysis at CMC, Vellore conducted by CMC, Wellcome Trust Research laboratory and CDC Atlanta USA, from 7th to 9th February 2017.

Training programme on Rotavirus Screening by ELISA and genotype detection by rt-PCR at NIE conducted by CMC, Wellcome Trust Research laboratory, Vellore and CDC Atlanta USA, on August 24-26, 2016.

National training programme on Electron microscopy for a period of one week from 03.01.2011 to 08.01.2011 in the department of Electron microscopy Cancer institute (WIA), Chennai.

Technical Skills:-

Molecular biology: - Flow cytometry and Sequencing

cDNA library construction, genotype detection.

Cloning and gene expression

Purification and analysis of recombinant protein

Polymerase Chain Reaction (PCR) and RT- PCR

Restriction Fragment Length Polymorphism (RFLP)

Electroporation,Polyacrylamide gel electrophoresis.

Immunology: - Western blotting, ELISA, in-situ DNA hybridization.

Microbiology: - Microbial screening from water and Human specimen, Isolation and

molecular identification of microbes are using 16SrRNA.

Tangential flow filtration, Ultra centrifugation. Haemocytometer.

Epifluorescence Microscopy, Metagenomic analysis.

Publication:-

Satheesh Kumar. S, Chandrasekar Bal Amarnath, Prithiviraj Nagarajan, Prabhudas Nelaturi, Vishnu Bhat Ballambattu, Sambandam Ravikumar. Phylogeograohic and genetic analyses of novel COVID-19 reveals divergence with other coronavirus family. journal of Xidian University, Elsvier publication P-4-7, July 2020

Satheesh Kumar. S, R. Ananda Bharathi, J.J.S. Rajan, S.V. Alavandi, V. Chitra, M.Muralidhar. Viability of white spot syndrome virus (WSSV) with reference to soil physico chemical properties. journal of Aquaculture International, Springer publication P-1-14, June 2019

S.V.Alavandi, R.Anandha Bharathi, S.Satheesh Kumar, N..Dinesh Kumar, C.Saravana kumar, Joseph Sahayarajan. Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water. Journal of Virological methods,March 2015

Satheesh Kumar. S, R. Ananda Bharathi, J.J.S. Rajan, S.V. Alavandi, M. Poornima, C.P. Balasubramanian, A.G. Ponniah. Viability of white spot syndrome virus (WSSV) in sediment during sun-drying (drainable pond) and under non-drainable pond conditions indicated by infectivity to shrimp. Aquaculture 402-403 (2013) 119–126

Ramesh kumar D, Sanjuktha M, Singaravel R1, Ramakrishnan S, Rajan J.J.S, Satheesh kumar S, Poornima M*, Santiago T.C2, Alavandi S.V Molecular modelling and Docking studies on shrimp vitellogenin receptor and ligand target mediated delivery system. International Journal of Research in Drug Delivery 2012; 2(1): 11-14.

Satheesh Kumar. S, R. Ananda Bharathi, J.J.S. Rajan, C.P. Balasubramanian, S.V. Alavandi, . Role of bivalvemollusk (Meretrix meretrix) in the transmission of white spot syndrome virus (WSSV) in shrimp. Manuscript submitted to journal of Virology (Manuscript under review)

Manuscript under preparation and about to submit

C.P.Girish Kumar., Satheesh Kumar, S. Venkatasubramanian A.Sree devi Narayanan, Priya darshini, R.Ruban raj. Prevalence of severe rotavirus associated gastroenteritis among children under five years of age in Chennai, India.

C.P.Girish Kumar, Satheesh Kumar, S. venkatasubramanian A.Sree devi Narayanan, Priya darshini, R.Ruban raj. Molecular diversity of rotavirus from children with acute gastroenteritis in and around Chennai, India.

Satheesh Kumar. S, R. Ananda Bharathi, J.J.S. Rajan, C.P. Balasubramanian, S.V. Alavandi, . Role of microalgae (Chlorella salina) in the transmission of white spot syndrome virus (WSSV) in shrimp. Manuscript under preparation.

Paper Presentation:-

Satheesh Kumar S., Ananda Bharathi, R., J.J,S.Rajan., C.P. balasubramanian, Alavandi, S.V.2019. Role of bivalvemollusk (Meretrix meretrix) in the transmission of white spot syndrome virus (WSSV) in shrimp . World International conference on viruses to genomes, 2019, Jan 23-25, ICAR-CIBA, Chennai, Abstract#HLT 57, P-160.

Satheesh Kumar S., Ananda Bharathi, R., Chitra., J.J,S.Rajan., Alavandi, S.V.2017. WSSV viability in shrimp pond sediments with reference to physicochemical properties. National conference on viruses to virpomes, Virocon 2017, Dec 07-09, NITTE university, Mangalore, Abstract#19, P-179.

Satheesh Kumar S., Ananda Bharathi, R., Alavandi, S.V., Poornima.M. Kalaimani, N.2012..Detection of WSSV and other Shrimp viral pathogens in water from brackish water ecosystems. National conference on Biotechnological Approaches in Aquaculture, Live Aqua 2012 February 1-3,Bharathiyar University,Coimbatore,Tamil Nadu,Abstract #21, pp18-19.

Conference Proceeding:-

1. National symposium on viruses to viromes at virocon 2017 conducted by NITTE University, Mangalore on December 07 to 09, 2107

2. Ananda Bharathi, R., Satheesh Kumar, S., Alavandi, S.V., Poornima.M. Kalaimani, N.2012.Viral abundance in brackish water aquaculture ecosystems. National conference on new vistas in Indian Aquaculture- Book of Abstracts. Coastal Aquaculture society of india,Chennai and Central institute of Brackish water Aquaculture,Chennai,23-24th February 2012,Abstract # HM 0-8, pp 48.

3. National symposium on problems of Anemia in India conducted by Genetics and Environment Dept, sponsored by Medical council of India.

Computer Skills:-

Knowledge in MS Word, Excel, Photoshop, Gene tool, Clustal – X, Gene Doc, Bioedit, Internet, NCBI blast, phylogenetic tree constructions and primer designing,etc.,

Personal Details:-

Date of Birth : 16th March 1981

Gender : Male

Martial Status : Married

Passport No : H4932224

Contact Address:-

# NO 9, 1st street, Seemathamman nagar, Madhuravoyal, Chennai-600095

Mobile: +91-994*******

Email: admwhl@r.postjobfree.com

Declaration

I hereby declare that the above-mentioned details are true to the best of my knowledge.

S.Satheesh Kumar

Station: Chennai.

Date: 14-05-2021

References:-

Dr. S.V. Alavandi, Ph.D (Microbiology)

Principal Scientist & Guide

Head, AquaticAnimalHealth&EnvironmentDivision,

ICAR- Central Institute of Brackish water Aquaculture (CIBA),

75,Santhome high road, R.A. puram, Chennai-28.

Email: admwhl@r.postjobfree.com

Dr. C.P.Balasubramanian. Ph.D (Animal Pathology)

Principal Scientist & Co-guide

SIC, Crustacean Culture Division

ICAR- Central Institute of Brackish water Aquaculture (CIBA),

75,Santhome high road, R.A. puram, Chennai-28.

Email: admwhl@r.postjobfree.com

Dr.C.P.Girish kumar, Ph.D (Microbiology).

Scientist E

National Institute of Epidemiology (NIE)

Indian Council of medical Research (ICMR)

Ayapakkam, Chennai

Email: admwhl@r.postjobfree.com

Dr. S, Ravikumar

Principal Scientist

Aarupadai Veedu Medical college and Hospital

Biomedical Research Centre

Central Research laboratory

Puducherry

Email: admwhl@r.postjobfree.com

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