IMMIGRATION STATUS: PERMANENT RESIDENT (GREEN CARD)

EDUCATION

• Ohio University, USA, Doctor of Philosophy in Biochemistry Aug 2015

• University of Peshawar, Pakistan, Bachelor of Science in Biochemistry Feb 1996 PROFESSIONAL EXPERIENCE

• Postdoctoral Fellow, Yale University, New Haven, CT, USA May 2020 – Present

• Postdoctoral Associate, Yale University, New Haven, CT, USA May 2018 – Apr 2020

• Scientific writer, Ohio University, Athens, OH, USA Jan 2017 – Dec 2017

• Visiting Scholar, University of Colorado, Denver, USA Feb 2016 – Dec 2016

• Postdoctoral Associate, University of Georgia, Athens, Georgia, USA Aug 2015 – Jan 2016

• Graduate Teaching Assistant, Ohio University, Athens, OH, USA Jan 2010 – Aug 2015 TEACHING EXPERIENCE

Labs Taught:

• CHEM 1210: Principles of Chemistry-I

• CHEM 1220: Principles of Chemistry-II

• CHEM 1500: Concepts in Chemistry

• CHEM 1510: Fundamentals of Chemistry-I

• CHEM 1520: Fundamentals of Chemistry-II

Exams Graded:

• CHEM 1210: Principles of Chemistry-I

• CHEM 1220: Principles of Chemistry-II

• CHEM 1500: Concepts in Chemistry

• CHEM 1510: Fundamentals of Chemistry-I

• CHEM 1520: Fundamentals of Chemistry-II.

Alamzeb Khan, PhD

Department of Vascular Biology and Therapeutics

& Department of Pediatrics

Yale School of Medicine, Yale University

10 Amistad St. Amistad Building

New Haven, Connecticut, 06520

Cell: 303-***-****

Fax : 203-***-****

Email: admsyw@r.postjobfree.com

https://www.linkedin.com/in/alamzeb-khan-phd-13918038/ RESEARCH INTERESTS

Endothelial Permeability, Capillary Leak, Endothelial Barrier Function, Capillary Permeability Vascular Inflammation, Vascular Endothelium, Vascular biology; Blood Vessels; Endothelium; Sepsis; Capillary Leak Syndrome; Microvascular endothelium; Nitric oxide; Peroxynitrite; superoxide. RESEARCH EXPERIENCES

PhD Research Assistant:

Nanomedical Research Laboratory, Ohio University, Athens, OH, USA

• Studied nitric oxide (NO) and peroxynitrite (ONOO-) imbalance in dysfunctional endothelium in cellular models of hypertension and diabetes and investigated the effect of vitamin D3 on the restoration of

[NO]/[ONOO-] imbalance.

• Used nano-analytical approach (nano-sensors for detecting NO and ONOO-) and measured in real-time the release of NO and ONOO- in HUVECs in cellular models of hypertension and diabetes.

• Used molecular and cell biology techniques and investigated the effect of vitamin D3 on the translational regulation of e-NOS, i-NOS and NADPH oxidases-4 in cellular models of hypertension and diabetes. Postdoctoral Associate / Postdoctoral Fellow:

Yale School of Medicine, Yale University, New Haven, CT, USA

• Measures trans-endothelial electrical resistance (TEER) – a quantitative technique to measure the integrity of tight junction dynamics in cell culture models of inflammation.

• Interrogates cellular signalling events related to endothelial permeability changes through western blot, qRT-PCR, siRNA knockdown and pharmacological manipulation of endothelial cells. Scientific Writer:

Nanomedical Research Laboratory, Ohio University, Athens, OH, USA

• Measured in real-time release of nitric oxide, and peroxynitrite in cell culture models.

• Analysed, interpreted, and calculated scientific data, wrote, and published manuscript in the International journal of Nanomedicine.

• Trained graduate students and participated in research projects of other graduate students Visiting Scholar:

Anschutz Medical Campus, University of Colorado Denver, Aurora, USA

• Introduced new technologies for measuring reactive oxygen and nitrogen species in cell culture and animal models.

• Reviewed scientific publications for international journals.

• Helped graduate students with their project-related experiments and worked closely with postdoctoral research associates in their projects.

• Worked with MD fellows, lab technicians and junior faculty members.

• Brought unique skills in NO biology and contributed to the ongoing research in the laboratory related to oxidative stress and pulmonary hypertension.

Postdoctoral Associate:

College of Pharmacy, University of Georgia, Athens, Georgia

• Investigated approaches for targeting post-translational modifications of proteins in castration resistant prostate cancer.

• Suppressed the proliferation of endothelial cells in then inhibition of prostate tumorigenesis.

• Communicated regularly and in a timely fashion with the PI regarding research and other issues relevant to the operation of the laboratory.

• Assisted with the supervision of junior members of the laboratory PROFESSIONAL SKILLS

Proteomics/Cell Biology/Molecular Biology:

• Mammalian Cell culture/Cell passaging,

• Isolation of Human Dermal Microvascular Endothelial Cells (HDMECs) using MACs CD31 Microbead

• Protein extraction

• BCA/Bradford Assay,

• Western blotting, ELISA

• Agarose gel electrophoresis, Polyacrylamide gel electrophoresis (PAGE),

• Confocal/Fluorescent Microscopy,

• Bacterial Transformation/Colony Screening

• siRNA Transfection

• Lentivirus/Retrovirus Transduction

• Lentivirus Production

• Preparation of competent cells, Growth cycle of bacteria, Colony screening.

• Organelle fractionation,

• Cell counting (Haemocytometer)

• LDL/HDL Separation by gradient centrifugation, UV-spectroscopy

• Protein Pull-down Assays/Immunoprecipitation of Proteins/Transwells Assay

• Immuno-histochemistry, Immuno-cytochemistry

Genomics:

• PCR, RT-PCR, qRT-PCR,

• DNA and RNA purification

• Plasmid DNA purification, minipreps, Maxi-Prep,

• Gene Cloning, Ligation reactions, Restriction Enzymes digestion,

• Primers designing

• Immortalization of HDMECs using Retrovirus carrying h-TERT and SV40 Large T-Antigen Analytical Skills:

• Preparation of Nitric Oxide (NO) and Peroxynitrite (ONOO-) Nano-sensors,

• In vivo, ex vivo and in vitro Measurements of Nitric Oxide and Peroxynitrite by amperometry

• Measurement of Nitric Oxide and Peroxynitrite standards by UV-spectrophotometry.

• Electrical Cell Impendence Sensing (ECIS) for measuring cellular Trans-endothelial electrical resistance (TEER).

Animal Models:

• Spontaneous Hypertensive Rats (SHR), Normotensive Wistar Kyota Rats (WKY), Prostate Generation Assay.

AWARDS/HONORS

• First Class First Division throughout academic career 1992-1998

• District Scholarship Award, Government Postgraduate College Charsadda, Pakistan 1998

• Merit Scholarship Award for Undergraduate Studies, Government Postgraduate College 1995

• Charsadda, Pakistan

Faculty of Arts Graduate Award, University of British Columbia, British Columbia 2017

• Canada

FELLOWSHIP/GRANT AWARD

NIH/T32 training grant fellowship (5T32HL007974-20)

• Supported by Department of Laboratory Medicine, Immunohematology and Transfusion Medicine Training Grant, Yale School of Medicine, Yale University TALKS/PRESENTATIONS

2018 Calcitriol Reverses Nitric Oxide and Peroxynitrite Imbalance in Dysfunctional Endothelium: a nanomedical approach, Department of Biochemistry, Quaid-e-Azam University, Islamabad, Pakistan. 2018 Calcitriol Reverses Nitric Oxide and Peroxynitrite Imbalance in Dysfunctional Endothelium: a nanomedical approach, Department of Biochemistry, Khyber Medical University, Islamabad, Pakistan 2017 Nanomedical Studies of Vitamin D3 restoration of Nitric Oxide/Peroxynitrite Balance in Dysfunctional Endothelium – Clinical Implications for Cardiovascular Diseases, Department of Pediatrics Critical Care Medicine, School of Medicine, Yale University, Connecticut, New Havens 2014 The Beneficial effect of 1 α25 Dihydroxyvitamin D3 on the restoration of nitric oxide and peroxynitrite in dysfunctional endothelium. Membrane-Molecular and Cellular Biology Program, Konneker Research Labs, Ohio University, Athens Ohio

2014 The effect of angiotensin-II-Induced generation of oxidative stress on endothelium. Membrane-Molecular and Cellular Biology Program, Konneker Research Labs, The Ridges, Ohio University, Athens Ohio 2013 The beneficial effect of 1 α25 Dihydroxyvitamin D3 on Dysfunctional Endothelium. Chemistry and Biochemistry Group (cBIG) Seminar, Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio

2012 Reversing nitric oxide and peroxynitrite imbalance in dysfunctional endothelium. Chemistry and Biochemistry Group (cBIG) Seminar, Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio

2011 The Role of nitric oxide and peroxynitrite in the pathophysiology of migraine headache. Chemistry and Biochemistry Group (cBIG) Seminar, Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio.

PUBLICATIONS

• Khan A, Dawoud H, Malinski T. Nanomedical studies of the restoration of nitric oxide/peroxynitrite balance in dysfunctional endothelium by 1,25-dihydroxy vitamin D3 - clinical implications for cardiovascular diseases. Int J Nanomedicine. 2018 Jan 19 ;13:455–66.

• Ramaswamy A, Brodsky NN, Sumida TS, Comi M, Asashima H, Hoehn KB, Khan A et al. Post- infectious inflammatory disease in MIS-C features elevated cytotoxicity signatures and autoreactivity that correlates with severity. medRxiv; 2020.

• Khan A, Ni W, Lopez Giraldez F, Kluger MS, Pober JS, Pierce RW. Tumor necrosis factor induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption. FASEB J [Internet]. 2021 Jun 4 [cited 2021 May 5];35(6):e21627. Available from: https://onlinelibrary.wiley.com/doi/10.1096/fj.202002783RR MANUSCRIPT UNDER PREPARATION

• A. Khan, and R. Pierce, TNF-induced ARHGEF12 acts as a specific GEF for Rap1A in human endothelial cells and reverses tight junction disruption and barrier breakdown. Under preparation, and to be submitted to Circulation Research.

• A. Khan and T. Malinski, 1 α25 Dihydroxyvitamin D3 improves Nitric oxide and decreases nitroxidative stresses in hyperglycemic-induced dysfunctional endothelium. To be submitted to Clinical Diabetes

(Journal of American Diabetic Association).

MEMBERSHIP

• Member of North American Vascular Biology Organization, USA CONFERENCES AND SEMINARS

• Alamzeb Khan et al. ARHGEF10 acts as a GEF for RhoB in controlling capillary endothelial permeability. ePoster presented at the North American Vascular Biology Organization, Germantown, MD 20874-2211 USA (2020)

• Alamzeb Khan et al. ARHGEF12 acts as a GEF for Rap1A in controlling capillary endothelial permeability. ePoster presented at the North American Vascular Biology Organization, Germantown, MD 20874-2211 USA (2020)

• Alamzeb Khan et al. ARHGEF10 acts as a GEF for RhoB in controlling capillary endothelial permeability. Poster presented at the Department of Pediatrics, and Cell Biology School of Medicine, Yale University, USA (2019)

COMPUTER AND OTHER SKILLS

• OriginLab for Data Analysis

• Microsoft Word, Excel, Power Point

• Windows 10

• Prism

• And a bunch of other software.

REFERENCES

• Dr. Tadeusz Malinski (PhD advisor)

Distinguished Professor, Department of Chemistry and Biochemistry, Ohio University, Athens Ohio 45701, Tel: 740-***-****/740-***-****; Fax: 740-***-****; Email: admsyw@r.postjobfree.com

• Dr. John J. Kopchick

Distinguished Professor & Goll-Ohio Eminent Scholar, Edison Biotechnology Institute & Dept. of Biomedical Sciences, HCOM, Ohio University, Athens, Ohio 45701, Tel: 740-***-****, Email: admsyw@r.postjobfree.com

• Dr. Rebecca Barlag

Professor of Instruction and Director of B.S. Forensic Chemistry, Department of Chemistry and Biochemistry, Ohio University, Athens Ohio 45701, Tel: 740-***-****/740-***-****; Fax: 740-***-****; Email: admsyw@r.postjobfree.com

END

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