Bingqiang Wen

Gender: Male

Nationality: China

Tel : 513-***-****

Email : adm88p@r.postjobfree.com adm88p@r.postjobfree.com Address 141 LOUIS AVE, APT3

CINCINNATI, OH, 45220

EDUCATION AND PROJECTE

2017.8 up to the present : Research Fellow, Cincinnati Children’s Hospital Medical Center Project: The Use of Mouse-Mouse and Mouse-Rat Chimeras to Generate Lung Tissue from Embryonic Stem Cells.

2012.9 - 2017.6: Doctor degree in Physiology, China Agricultural University Project: The study on tetraploid ESCs pluripotency. Project: The study on the role of Krt8 in the mouse preimplantation embryos. Cooperative Project: The effect of iPSC mitochondrial supplement on the development and methylation status of imprinted genes in early stage mouse embryos. 2009.9 - 2012.6: Master degree in Animal Genetics, Breeding and Reproduction Science, Hebei Agricultural University

Project: The study on the effect of histone deacetylase inhibitor scriptaid on sheep somatic cell nuclear transplantation embryos

2005.9 – 2009.6: Bachelor degree in Traditional Chinese Medicine, Hebei Agricultural University

RESEARCH TECHNIQUES AND SKILLS

Mouse oocyte/embryo techniques: oocyte assessment, oocyte cryopreservation, in vitro maturation (IVM), in vitro fertilization (IVF), in vitro culture (IVC), micromanipulation (SCNT, ICSI, plasmid microinjection, mRNA and siRNA intracytoplasmic injection, pronuclear removal, ES cells injection), separate embryo blastomeres, separate ICM cells and TE cells, embryo grade evaluation, embryo transfer.

Cell techniques: ES cell line establishment, TS cell line establishment, iPS cell line establishment, cell transfection, cell infection.

Molecular biological techniques: PCR, qRT-PCR, vector construction, gene knockdown, gene overexpression, gene knockout (CRISPR-Cas9), immunohistochemistry, immunofluorescence histochemical, Western Blot, Bisulfite sequencing, FACS, co-immunoprecipitation etc.. JOURNAL LIST

1. Wen B, Li E, Ustiyan V, Wang G, Guo M, Na CL, Kalin GT, Galvan V, Xu Y, Weaver TE et al. 2021. In Vivo Generation of Lung and Thyroid Tissues from Embryonic Stem Cells Using Blastocyst Complementation. Am J Respir Crit Care Med 203: 471-483 2. Wang G*, Wen B*, Ren X, Li E, Zhang Y, Guo M, Xu Y, Whitsett JA, Kalin TV, Kalinichenko VV. 2021. Generation of Pulmonary Endothelial Progenitor Cells for Cell-Based Therapy Using Interspecies Mouse-Rat Chimeras. Am J Respir Crit Care Med (doi:10.1164/rccm.202003- 0758OC) (Co-first)

3. Wen, B*, Li, R*, Cheng, K, Li, E, Zhang, S, Xiang, J, Wang, Y, & Han, J. (2017). Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues. Scientific reports 7(1): 3030. (Co-first) 4. Li R*, Wen B*, Zhao H, Ouyang N, Ou S, Wang W, Han J, Yang D. 2017. Embryo development after mitochondrial supplementation from induced pluripotent stem cells. J Assist Reprod Genet doi:10.1007/s10815-017-0948-9. (Co-first)

5. Wen, B*, Li, J*, Li, J, Tian, S, Sun, S, Qi, X, Cai, W, & Chang, Q. (2014). The histone deacetylase inhibitor Scriptaid improves in vitro developmental competence of ovine somatic cell nuclear transferred embryos. Theriogenology 81, 332-339. (Co-first) 6. Li E, Ustiyan V, Wen B, Kalin GT, Whitsett JA, Kalin TV, Kalinichenko VV. 2021. Blastocyst complementation reveals that NKX2-1 establishes the proximal-peripheral boundary of the airway epithelium. Developmental dynamics : an official publication of the American Association of Anatomists doi:10.1002/dvdy.298.

7. Cappelletti M, Doll JR, Stankiewicz TE, Lawson MJ, Sauer V, Wen B, Kalinichenko VV, Sun X, Tilburgs T, Divanovic S. 2020. Maternal regulation of inflammatory cues is required for induction of preterm birth. JCI Insight 5.

8. Yue L, Pei Y, Zhong L, Yang H, Wang Y, Zhang W, Chen N, Zhu Q, Gao J, Zhi M Wen B et al. 2020. Mthfd2 Modulates Mitochondrial Function and DNA Repair to Maintain the Pluripotency of Mouse Stem Cells. Stem Cell Reports doi:10.1016/j.stemcr.2020.06.018. 9. Zhong, L, Mu, H, Wen, B, Zhang, W, Wei, Q, Cao, S. & Han, J. (2018). Identification of porcine long non-coding RNA involved in the regulatory network during pre-implantation embryonic development and somatic reprogramming. Scientific reports 8(1): 6649. 10. Xiang J, Cao S, Zhong L, Wang H, Pei Y, Wei Q, Wen B, Mu H, Zhang S, Yue L et al. 2017. Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos. Protein Cell doi:10.1007/s13238-017-0470-y. 11. Wang, Y, Li, J, Xiang, J, Wen, B, Mu, H, Zhang, W. & Han, J. (2016). Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs. Protein Cell 7(2): 152-156.

12. Pei, Y, Yue, L, Zhang, W, Wang, Y, Wen, B, Zhong, L, Xiang, J, Li, J, Zhang, S, Wang, H, Mu, H, Wei, Q & Han, J. (2015). Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming. Scientific reports 5: 16539. 13. Zhang, W, Pei, Y, Zhong, L, Wen, B, Cao, S, & Han, J. (2015). Pluripotent and Metabolic Features of Two Types of Porcine iPSCs Derived from Defined Mouse and Human ES Cell Culture Conditions. PLoS One 10, e124562.

14. Cao, H. Su, W. Li, J. Wen, B, Tian, S, Sun S, Li, L, Wang, H, & Li, J. (2015). Effects of Scriptaid on Cell Cycle and Histone Acetylation of Ovine Nuclear Donor Cumulus Cells and their Ability to Support the Development of Somatic Cell Nuclear Transfer Embryos. Journal of Microbiology and Biotechnology 4(3): 15-21.

HONORS AND AWARDS

2013: Excellent master's thesis of Hebei Province

2014: Second Academic Scholarships

2015: First Academic Scholarships

2019: Young Investigator award of 2019 ATS Grover conference

Contact this candidate