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Biotechnology And Biochemistry

Location:
Qibla, Kuwait City, Kuwait
Posted:
April 16, 2021

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Resume:

Communication Address :

VIMAL KUMAR PERUMAL

Flat No. 10, Building No. 139, Block No. 1, Street No.91, Farwaniyah - 85401, Kuwait.

Mobile No. +965-********

WhatsApp No.+91-989*******

Email :- adlqda@r.postjobfree.com

Summary of Qualifications:

Over all 13 years of experience in Biochemistry(Protein Purification/Clinical), Biotechnology, Microbiology and QA/QC

Strong background in Clinical and Technical analysis

Proven skills as a good team player with exemplary analytical and problem solving

Demonstrated ability to perform any kind of analytical methods in Biochemistry, Biotechnology, Microbiology and QA/QC

Professional communication skills includes fluency in English, Tamil, Hindi, Arabic

Master of Science in Biotechnology (June 2008 – May 2010)

Bachelor of Science in Biotechnology (June 2004 – May 2007)

Computer skills include - Wwindows XP, internet, and Microsoft office – Word, Excel and Power point.

PROFESSIONAL EXPERIENCE:

Laboratory Technician – Biochemistry from 2014 till Date

Ministry of Health (Under MOH) - Farwaniyah Hospital, Kuwait.

Executed and analyzed tests in areas including Biochemistry, hematology, Microbiology, and bacteriology to aid physicians in diagnosing and treating disease.

Skilled in medical lab technician tasks including sample collection, analysis and lab report production

Knowledgeable of state approved medical lab policies, hygiene requirements and general/standard procedures

Consistently commended for the timely, high quality completion of both routine and special laboratory assays of patients specimen.

Ensured test result validity before recording / reporting results earning a reputation for meticulous attention to detail.

Demonstrated the ability to communicate the test results effectively with physicians and pathologists.

Evaluated quality control with in laboratory using standard laboratory test and measurements controls, and maintain OSHA, Safety and Risk management guidelines.

In-house preparation of diagnostic reagent for biochemical analysis such as glucose, creatinine, protein, albumin, phosphorus and calcium.

RESEARCH AND DEVELOPMENT IN BIOTECHNOLOGY from 2007 until 2013

Laboratory technician Biotechnology and QA /QC

Tirumalai Chemicals Limited - Vellore, Tamilnadu, India.

Laboratory experience in protein electrophoresis

Biochemical analysis of sugars, proteins amino acids by qualitative and quantitative methods

Native and SDS – PAGE (CBB staining, silver staining and zonogram analysis)

Purification and identification of Amino acids & Protein using Chromatography (Column, TLC and Paper).

Enzyme Immobilization

Micro biology Laboratory

Laboratory experience in Aseptic techniques like Culture maintenance of mother culture, started culture and strains.

Isolations of microorganism from soil, water analysis and environmental air monitoring processes.

Anti-microbial susceptibility testing techniques including antibiotics

Checking the growth of organism with the inoculants (Turbidity method)

Determination of viable count (MPN method)

Identification test of environmental swabbing.

Microbial limit test for the raw materials and in process samples.

Isolation and identification of plate count microorganisms

Analyzing various samples for the presence of microorganisms.

Culture Handled

Nocardia Tartaricans

Arthrobacter

Rhodobacter

Upstream Techniques

Culture Development & Maintenance:

Culture development by Inoculum Development-from slant to seed culture.

Inoculum development from seed to pilot level for fermentation process

Culture maintenance were done by making Glycerol stock and slants

Documentation, maintenance and updating of SOP

Downstream Techniques

Centrifugation and separation of biomass using REMI and PENWALT.

Filtration was done through Micro filtration.

Cell disruption techniques (Homogenizer and Sonicator)

Purification of enzymes both intracellular and extracellular

Protein precipitation using solvent and salting out methods

Enzymology

Whole cell Enzyme Immobilization (Hydrolase enzyme from Lab to Production scale )

Enzyme Immobilization (Lactate Dehydrogenase, Lactase, Lipase, Peroxidase & Glutamate Dehydrogenase)

Dual Enzyme Immobilization (Leucine Dehydrogenase & Formate Dehydrogenase)

LAB CHEMIST

Analysis of DM Plant water samples, Boiler water samples, Cooling tower water samples, Effluent water samples.

Analysis of feed water samples (pH, conductivity, Silica, Hydrazine, Morpholine, chlorine, Iron, Total Hardness, Phosphate, TSS, SDI, MLVS, TDS, DO, Sulphate)

Analysis of ETP water samples (pH, COD, BOD, TDS, Sulphate, chloride, alkalinity, and total hardness.

Analysis of o-xylene and its impurities by Gas Chromatography

Analysis of intermediate samples

Analysis of crude phthalic anhydride and pure phthalic anhydride.

Analysis of oil samples. (Density, Moisture, viscosity, acidity)

Analysis of scale samples

ADDITIONAL RESPONSIBILITIES INCLUDES:

Controlling Process intermediate quality Parameters through continual Monitoring.

Implementing and Maintaining of Quality Management system ISO.9001/14000.

Keeping the Laboratory very systematic and Hygienic condition by implementing 5S Activities through TPM. Safety and performance presentation

Safety and performance presentation

Upgrading Product Quality through implementing TPM concept.

Calibration and Validation of all instruments and analysis methods

Regular maintenance of analytical instruments

Documentation.

Training and guiding of new trainees

Maintaining personal and industrial safety

Co- ordination with process engineers

Trouble shooting in analysis.

Instruments Handled / Analytical Techniques

Gas Chromatography –

Tangential flow filtration (Minimate and Centramate) and all other filtration aids.

Centrifuge (Thermo scientific)

Sonicator

SDS PAGE for protein profiling and molecular weight determinations

High Performance Liquid Chromatography –

UV – Visible Spectrophotometer

Lyophilization.

Flame photometer.

Cannon Fensky Viscometer

Karl Fisher Titrates

IR Analyzer

pH Meter

Conductivity Meter

Dew Point Apparatus

EDUCATIONAL DETAILS

Master of Science in Biotechnology (June 2008 – May 2010)

Periyar University, Salem, Tamilnadu, India.

Bachelor of Science in Biotechnology (June 2004 – May 2007)

VIT University, Vellore, Tamilnadu, India.

PERSONAL DETAILS

Date of Birth : 25/05/1987

Nationality : Indian

Marital status : Married

Languages known : English (Speak, Read and Write)

Tamil (Speak, Read and Write)

Hindi (Speak)

PASSPORT DETAILS

Passport No : M9886266

Place of issue : Kuwait

Validity : 06/12/2015 till 05/12/2025

Permanent Address : No. 50, S.K.M Garden, Jaga Jothi Nagar Extension,

Alamelumangapuram, Vellore – District,

Tamilnadu- 632009, India.



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