Molecular Microbiology

Location:

Tehran, Iran

Posted:

March 30, 2021

Contact this candidate

Resume:

Rooh Kheiri Vita

E-mail: *.*******@**.**.**

Cell Phone: +989*********

Professions

●Bacteriology Expert

Alborz Province Water and Wastewater (private sector). IRI, Oct 2003 - Present.

●Molecular microbiology expert

Alborz Province Water and Wastewater (private sector). May 2009 – Present. PCR, primer and probe designing, Multiplex PCR setting and optimization.

Researcher for Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. 2010-present.

●Chemical tests analyzer

●Metagenomics analyzer

Researcher for Molecular and Metagenomics analyzer, Tehran University, Iran. 2018-present. Bioinformatics soft wares such as R studio, QIIME, and Linux.

Education

●Tehran University, Ph.D., Microbiology, Sep 2018 - Present (sophomore) (The first rank of the University).

●Karaj Islamic Azad University, Masters, Microbiology, Sep 2009 - Dec 2012.

●Karaj Islamic Azad University, Bachelor, Microbiology, Sep 1998 - Jul 2002

Experiences

●I have great experiences in culture-based microbiology Based on my APHA 2017 including Heterotrophic plate count, Total coliforms, E. coli, fecal streptococci, Pseudomonas aeruginosa, LAL, and Bioburden testing.

●Performing molecular techniques such as DNA/RNA extraction, DNA isolation, electrophoresis, real time PCR, conventional PCR, plasmid isolation and characterization, Sanger sequencing.

●I have designed several multiplex qPCR& PCR kits for the simultaneous detection of bacteria and viruses. For example, one of my kits, a hexaplex one is designed for the environmental (water) samples which can specifically and simultaneously detect 6 targets including, Escherichia coli, Salmonella spp., Shigella spp., Enterococcus spp., Pseudomonas aeruginosa, and Vibrio cholera. My other designed PCR kits include

oOne Pentaplex for Shigella (to detect all genuses)

oTwo hexaplexes for pathotyping Escherichia coli

oOne Pentaplex for Enterococcus (to detect some important geneses)

oOne Hexaplex for Salmonella enterica (to detect some important serovars)

oOne Pentaplex for Vibrio cholera (to detect toxin, biotype and serogroup)

oDetecting Legionella by qPCR applying ISO TS 12869.

● Knowledge and prior hands-on experiences with molecular biology tools (including nucleic acid processing, qPCR and RT-qPCR assays, and next generation sequencing) and bioinformatics (genomic and metagenomics) tools are desirable. I am highly self-motivated and with excellent communication skills, and will work on research projects on wastewater surveillance of COVID-19 and other infectious diseases. It is noteworthy that I have developed the duplex qPCR kit for Coronavirus and Enterovirus detection.

●I have passed ISO/IEC 17025 courses and I am able to run the lab works based on ISO protocols. Therefore, I can Maintain standard requirements for ISO 17025 accreditation by working within the requirements of the NAFG Laboratory Quality Management System. I Exhibit technical competence in these areas, Accuracy of results established by analyzing a Certified Reference Material and Measure of uncertainty calculations utilized to verify adherence to requirements.

●I can apply some necessary quality control actions of a lab including UV irradiation efficiency and Culture media recovering test based on MC farland, Surface sterilization efficiency determination and so on.

●Knowledge of soft wares including Office tools to accurately enter testing data and results and statistically analyzing the results.

●Maintains open communication with supervisors and fellow employees to ensure optimal operation of the department.

●Working for a long time in water and wastewater laboratory, I have good experiences in chemistry tests such as pH, Electrical Conductivity, Total Suspended Solids (TSS), Total Dissolved Solids (TDS), BOD, COD, free residual chlorine, heavy metals (by Voltammetry polarography), and so on.

●As my Ph.D. thesis “Metagenomics analysis of hypersaline ecosystem”, I am studying the whole genomes of an extreme environment both cultured-based and metagenomics based (bioinformatics soft wares such as R studio, QIIME, and Linux).

Workshops

●Bioinformatics workshop (IBRC), Sep 2013

●Biostatistics workshop (IBRC), Aug 2013

●Molecular biology techniques workshop (IBRC), Sep 2012

●Microbial nutrition (IBRC), Dec 2012.

●Genetic engineering and gene cloning workshop (IBRC), May 2012

●Molecular phylogenetic analysis workshop (IBRC), Dec 2012

●Molecular diagnosis courses (Pasteur institute summer school), Sep 2012.

●Molecular detection of bacteria (Tehran University), 2017

●Phylogenetic analysis of prokaryotes (Tehran University), 2018

●ISO/IEC 17025 assessing (Tehran University), 2018

●Real time PCR (IBRC), 2019.

●Whole genome metagenomics analysis (Tehran University), 2019

●Linux and R studio (Tehran University), 2020

And so on

Personal characteristics

●Long time working as a lab supervisor makes me

oto work collaboratively, as a member of a team, to achieve a common goal

oThe flexibility and productivity to work in a fast paced, often varied environment

oA strong sense of pride in achieving accurate, timely, and consistent results in their work and perform other related tasks as needed

o Plan procedures for and direct special investigations and research; interpret and prepare reports of laboratory procedures, observations and results of tests; and develop and modify procedures and methods.

●As a teacher of the university I provide training in the area of expertise to laboratory personnel and visitors and provide technical support for developmental projects as needed, technical assistance in areas of: testing reagents, keeping records, and ordering media.

●Works closely with all laboratory personnel to ensure timely client service and accurate test results

Devices application

Device

Model

Usage

Incubators

different models of Memmert