neural differentiation of hES cells

Chang Hyun Lee, Ph.D.

****,**** **., **** *, Lubbock, TX., USA 79424 C: +1-806-***-**** E-mail: *********@*****.*** RESEARCH EXPERIENCES

****, **** – Present,

Postdoctoral research associate, Department of Chemical Engineering, Texas Tech University

Cardiac differentiation of H9 human ES cells

- Maintenance of human ES cells by high density mono- layer culture method.

- Cardiac differentiation of human and mouse ES cells.

Generation of Induced pluripotent stem cells using biopolymer (Elastin like polypeptide)

- FACS (Flow Jo), Immunocytochemistry (Nikon C2 confocal microscope)

- qRT-PCR (Quantstudio3, Applied biosystem)

- Gene delivery into fibroblast cells using biopolymer.

Neural differentiation of mouse and human embryonic stem cells.

- Dopaminergic neural differentiation of mouse ES cells using biopolymer.

3D culture of mouse and human ES cells and differentiation

- 3D differentiation of mouse and human ES cells

Covid19 research

- 293FT cell culture and Spike gene transfection and protein purification (Ni-NTA)

- Supplying VTM(virus transport media) of coronavirus testing to the local hospital and clinics

Microneedles for skin allergen immunotherapy

- Isolation of mRNA and analysis of cytokine expression in skin and spleen tissue using qRT-PCR.

- Splenocyte isolation from mouse spleen

- ELISA

- Cryosection of frozen tissue using Cryocut (Leica) and Immunohistochemistry

Microneedles for photodynamic therapy of skin tumors

- Skin tumor cell culture (A-20 cells)

2009.11 ~2013 .05

Researcher, Laboratory of Molecular Imaging, Department of Biomedical Science, CHA University

Molecular imaging using microRNA in mouse embryonic stem cells and cancer cells

- Operation of LSM710, 705 confocal microscopes (Carl Zeiss)

- Conjugation of Aptamer, oligonucleotide, peptide with nanoparticle (Fe3O4, Quantum dots)

- Gene delivery using Iron Nanoparticles, Fugene6 and Lipofectamine.

- Carbon dot synthesis and analysis (FTIR, XPS)

Molecular imaging and targeting using Aptamer in cancer cell, cancer stem cell and embryonic stem cells

Mouse hind –limb ischemia model production

- Molecular beacon-based bio sensing of microRNA126 during angiogenesis of EPC-treated ischemia.

2006. 07 ~ 2007. 08

MIRAE BIOTECH. CO., Ltd./Researcher

Mouse / Human ES Cells Culture and Maintenance

Neural Differentiation of human ES Cells and embryonic carcinoma cell (P19, Ntera2, mES and hES,)

Molecular Biology (PCR, QRT- PCR, Western blot and Gene Cloning)

2002. 08 ~ 2006. 06

MARIA Fertility Hospital / MARIA biotech/ Researcher

Mouse / Human ES Cells Culture and Maintenance

Differentiation of Mouse and Human ES Cells into Dopaminergic neuron

Transplantation of Disease-Model Animal and behavior test

- Production and verification of Parkinson’s disease animal model

- Production and verification of Huntington’s disease animal model

- Immunocytochemistry, Immunohistochemistry (DAB staining and fluorescence staining) and cryosection of frozen tissue

1998. 08 ~ 2002. 08

Microinjection of new foreign gene into porcine embryo

- Production of transgenic animal

EDUCATIONAL QUALIFICATION

2013.7 ~ present

Postdoctoral research associates

Department of Chemical Engineering, Texas Tech University 2006. 09 ~ 2012. 08

Degree of Doctor of Philosophy in Animal Biotechnology, Department of Animal Biotechnology, Konkuk University, Republic of Korea

Thesis: Nanoparticle-mediated efficient gene delivery into stem cells and generation of induced pluripotent stem cells

1999. 09 ~ 2001. 08

M.S. Department of Animal Husbandry, Konkuk University Thesis: Basic Study on Production of Transgenic Pig Harboring Cellulose Digestion Gene 1992. 02 ~ 1998. 02

B.S. Department of Animal Husbandry, Chonbuk University RESEARCH SKILL

Report Writing

Data collection

Time management

Problem-solving

Attention to detail

Creative thinker

Exceptional research skills

Academic positions and Honors

Academic

2013 - Present Postdoc research associate, Dept. of Chemical Engineering, Texas Tech University, Lubbock, TX

Industry

2002 - 2006 Researcher – MARIA Fertility Hospital / Medical Research Center, Seoul, Korea 1998 - 2002 Researcher- National Institute of Animal Science, Suwon, Korea Honors

2007 Science National Research Scholarship (The Korea Student Aid Foundation, KOSAF) 2007 Korean society of animal reproduction, Winner of Best Announcement Award 2007 Korean society of animal reproduction won the Best Poster Award Collaboration work

Dr. Anton M. Jetten, Dr. Kilsoo Jeon

Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Science, National Institutes of Health (NIEH/NIH), Research Triangle Park, NC 27709

- Glis3 induced dopaminergic neural differentiation of human ES cells.

- Crosslinked elastin-like polypeptides mediate direct cardiac differentiation of mouse embryonic and induced pluripotent stem cells without use of cardiac differentiation factors. Contribution to Science

Full publication list; (total 21 article, Total citation No.: 659

(https://scholar.google.com/citations?user=J1RfbiwAAAAJ&hl=en, 3/8/2021) C.H. Lee, H.S. Gill., (2019) Crosslinked elastin-like polypeptides mediate direct cardiac differentiation of embryonic and induced pluripotent stem cells without use of cardiac differentiation factors. Acta Biomaterials,2019, Preparing for Revision.

C.H. Lee, H.S. Gill., (2019) A New 3D mESCs Culture System and Characterization. 2019 in preparing patent applications. C.H. Lee, H.S. Gill., (2019) Generation of induced pluriportent stem cells using elastin like polypeptides as a non-viral gene delivery system Biochim Biophys Acta Mol Basis Dis. Feb 10 cited by 2 AK Shakya, CH Lee, MJ Uddin, HS Gill(2018) Assessment of Th1/Th2 Bias of STING Agonists Coated on Microneedles for Possible Use in Skin Allergen Immunotherapy Molecular pharmaceutics 15 (11), 5437-5443 cited by 3 AK Shakya, CH Lee, HS Gill (2018) Coated microneedle-based cutaneous immunotherapy prevents Der p 1– induced airway allergy in mice J. of Allergy and Clinical Immunology 142 (6), 2007-2011. e3 cited by 5 Akhilesh Kumar Shakya, Chang hyun Lee, and Harvinder Singh Gill (2017) Cutaneous vaccination with coated microneedles prevents development of airway allergy. J Control Release. 265, 75-82 cited by 18 Lee CH, Kim JH, Lee HJ, Jeon K, Lim H, Choi Hye, Lee ER, Park SH, Park JY, Hong S, Kim S, Cho SG. (2011) The generation of iPS cells using non-viral magnetic nanoparticle based transfection. Biomaterials. 32(28):6683- 91 PMID: 21683440 cited by 83

Lee CH, Kim EY, Jeon K, Tae JC, Lee KS, Kim YO, Jeong MY, Yun CW, Jeong DK, Cho SK, Kim JH, Lee HY, Riu KZ, Cho SG, Park SP. (2008) Simple, efficient, and reproducible gene transfection of mouse embryonic stem cells by magnetofection. Stem Cells Dev.,17(1):133-41. PMID: 18271700 cited by 50 Jain A.K., Lee C.H., Gill H.S. (2016) 5-Aminolevulinic acid coated microneedles for photodynamic therapy of skin tumors. J Control Release. 10; 239:72-81. PMID:27543445 cited by 34 Chang Hyun Lee, Jung il Chae, Hae Young Ko, Soonhag Kim (2013) Molecular beacon-based microRNA biosensor for imaging EPC-treated cellular therapy of ischemia. Journal of Molecular Imaging & Dynamics, 3:1 cited by 5 Eun-Hyung Noh, Hae Young Ko, Chang Hyun Lee, Myoung-Seok Jeong, Young Wook Chang, Soonhag Kim (2013) Carbon nanodot-based self-delivering microRNA sensor to visualize microRNA124a expression during neurogenesis Journal of Materials Chemistry B 1(35): 4438-4445 cited by 32 Lee CH, Rajendran R, Jeong MS, Ko HY, Joo JY, Cho S, Chang YW, Kim S. (2013) Bioimaging of targeting cancers using aptamer-conjugated carbon nanodots. Chem Commun., 25;49(58):6543-5. PMID: 2376499 cited by 54 Jo MH, Jeong MS, Ko HY, Lee CH, Kang WJ, Kim S. (2013) Radioisotope imaging of microRNA-9-regulating neurogenesis using sodium iodide symporter. Biomaterials. 34(20):4803-9. PMID: 23541638 cited by 9 Jo MH, Ali BA, Al-Khedhairy AA, Lee CH, Kim B, Haam S, Huh YM, Ko HY, Kim S. (2012) A reverse complementary multimodal imaging system to visualize microRNA9-involved neurogenesis using peptide targeting transferrin receptor- conjugated magnetic fluorescence nanoparticles. Biomaterials. 33(27):6456-67 PMID: 22727464 cited by 19 Mi-hee Jo, Chang Hyun Lee, Bahy A Ali, Saud A Alarifi, Abdulaziz A Al-Khedhairy, Soonhag Kim 2012 A bioinformatics approach for in vivo imaging of endogenous MicroRNA targets during neurogenesis. Tissue Engineering and Regenerative Medicine 9(3) 157-169 cited by 2

Kang WJ, Cho YL, Chae JR, Lee JD, Ali BA, Al-Khedhairy AA, Lee CH, Kim S. (2012) Dual optical biosensors for imaging microRNA-1 during myogenesis. Biomaterials. 33(27):6430-7. PMID: 22698721 cited by 30 Ham O, Song BW, Lee SY, Choi E, Cha MJ, Lee CY, Park JH, Kim IK, Chang W, Lim S, Lee CH, Kim S, Jang Y, Hwang KC. (2012) The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling. Biomaterials. 33(18):4500-7. PMID: 22449550 cited by 81 Ko HY, Choi KJ, Lee CH, Kim S. (2011) A multimodal nanoparticle-based cancer imaging probe simultaneously targeting nucleolin, integrin αvβ3 and tenascin-C proteins. Biomaterials. 32(4):1130-8. PMID: 21071077 cited by 81 Tae JC, Kim EY, Jeon K, Lee KS, Lee CH, Kim YO, Park SP, Kim NH. (2008) A MAPK pathway is involved in the control of cortical granule reaction and mitosis during bovine fertilization. Mol Reprod Dev., 75(8):1300-6 PMID: 18459151 cited by 13

Jeon K, Kim EY, Tae JC, Lee CH, Lee KS, Kim YO, Jeong DK, Cho SK, Kim JH, Lee HY, Riu KZ, Cho SG, Park SP.

(2008) Survivin protein expression in bovine follicular oocytes and their in vitro developmental competence. Anim Reprod Sci.,108(3-4):319-33. PMID: 17945442 cited by 20

K.S. Chung and J.H. Lim Park, S.P., E.Y. Kim, S.G. Kim, Y.J. Lee, K.S. Kil, S.Y. Park, J. Y. Yoon, C.H. Lee, In vitro Developmnet of Somatic Cell Nuclear Transferred Bovine Embryos Following Activation Timing in Enucleated and Cryopreserved MII Oocytes. Korean J. Animal Reprod.26(3): 245-252. PATENTS

BIOMATERIALS FOR 3D CELL GROWTH AND DIFFERENTIATION 2019, PCT Patent Application, Serial No.: PCT/US2019/056580

Method of manufacturing embryonic stem cell being introduced gene using nanoparticle. KR1020080010310A A composition for improving the proliferation ability and differentiation ability of induced pluripotent stem cells. KR1020090020115A

Transgenic porcine capable of producing human erythropoietin and preparation method thereof KR20010044610A

Oral and Poster presentation

CH Lee, AJ Shakya, and HS Gill, “Elastin like polypeptide enhances mouse stem cells differentiation into cardiomyocyte-like cells”, International Society for Stem Cell Research (ISSCR) Annual Meeting (June 2019), Los Angeles, CA, USA

Chang Hyun Lee, Harvinder Gill, "A New 3D mESC Culture System and Characterization", BMES Annual Meeting (Oct 2018), Atlanta, Georgia, USA

Chang Hyun Lee and Harvinder Singh Gill,"Generation of induced pluripotent stem cells using elastin like polypeptide”, International Society for Stem Cell Research (ISSCR) Annual meeting (June 2017), Boston, MA, USA Molecular imaging of a cancer-targeting theragnostics probe using a nucleolin aptamer- and microRNA-221 molecular beacon-conjugated nanoparticle, 2012, The Korean Cancer Association Efficient generation of safe induced pluripotent stem cells (iPSC) using magnet-based nanofection and comparative analysis of their transcriptomes and proteomes, 2010, The EMBO Meeting Generation and proof of induced pluripotent stem cells using nanofection and analysis, 2009, 34th FEBS Congress. Efficient Transfection into Embryonic Stem Cells Using Nanoparticles, 2007, The 19th Annual Meeting of the Korean Society for Molecular and Cellular Biology

Transplantation of the hES cell-derived NPC/OPCs into spinal cord injured rats, 2006, The Korean Society of Animal Reproduction.

Genetically Modified Human Embryonic Stem Cells Expressing Nurr1 and Their Differentiation into Tyrosine Hydroxylase Positive Cells in vitro, 2004, The Korean Society of Animal Reproduction. Genetically Modified Human Embryonic Stem Cells Relieve Symptomatic Motor Behavior in a Rat Model of Parkinson's Disease, 2003, The Korean Society of Animal Reproduction.

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