Dr. Mandeep Kaur, Ph.D. (Zoology/ Entomology/ Cytogenetics)

* ***** ******* **

Brampton, ON

CANADA

L6P1S1

E-mail: adkbj1@r.postjobfree.com

Phone: +1-437-***-****

D.O.B.: Dec 4, 1988

Objective

To succeed in an environment of growth and excellence which provides me job satisfaction, self development and help me to achieve my personal as well as organizational goals. Academic/ Career Profile

Year

Degree/Class/Career Institute

Grade / Status

2014-2020

2014

2013-2014

Ph.D.

Pre PhD Course

B.Ed.

Guru Nanak Dev

University, India

Guru Nanak Dev

University, India

Guru Nanak Dev

University, India

With publications

CGPA 9.56

63%

2009-2011 M.Sc. (Molecular

biology &

Biochemistry)

Guru Nanak Dev

University, India

65%

(Ist Division)

2006-2009

2005

2003

B.Sc. (Medical)

10+2 (Physics,

Chemistry, Biology)

Matriculation

Guru Nanak Dev

University, India

P.S.E.B.

P.S.E.B.

71%

(Ist Division)

61%

(Ist Division)

76%

(Ist Division)

Professional Research Experience

PhD- Biochemical, Genotoxic and Cytotoxic effects of Entomopathogenic Fungi on Spodoptera litura (Fabricius) 2014- 2020 (Supervisor Dr. Pooja chadha) Antioxidant and antiproliferative activity of Tinospora cordifolia plant 2010-2011 extract on C6 glioma cell lines

-M.Sc. Project (Supervisor- Dr. Jatinder Singh)

Mandeep Kaur, PhD

Mentoring Experience

1. Trained junior PhD students in lab.

2. Trained/Mentored M.Sc. project students in India. Teaching Experience

Teaching Entomology and Zoology to M.Sc. students. Academic Honors, Awards and Fellowships

1. Best poster award in national seminar on “Recent trends in Plant and Environmental Sciences”. Guru Nanak Dev University, Amritsar (Punjab) India. 2. Qualified National eligibility test (NET) for Lectureship conducted jointly by Council of Scientific and Industrial Research (CSIR) and University Grants Commission (UGC)- New Delhi, in June 2013.

3. Awarded Merit Certificate awards during B.Sc. and M.Sc. 4. Qualified Ph.D. eligibility of University three times (Toped one time). 5. Awarded with international travel grant by SERB and CSIR-UGC which provided me an opportunity to present research in an international conference in Canada. Publications (Peer Reviewed)

1. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur, Arun Kumar Yadav, Ramandeep Kaur, Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura, Nature- Scientific reports, 2018, 8, 4693. Impact factor 4.2

2. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur, Arun Kumar Yadav, Ramandeep Kaur, Evaluation of Genotoxic and Cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura, Journal of applied microbiology, 2019, 126, 881-893. Impact factor 3.1. 3. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Prince Sharma, Effect of Aspergillus flavus on Glutathione-S-Transferase activity of Spodoptera litura, Asian journal of microbiology and biotechnology, 2018, NAAS Rating 4.93 4. Prince Sharma, Pooja Chadha, Mandeep Kaur. Organic flame retardant induced oxidative stress in various organs of freshwater fish Channa punctatus. Pollution Research, 2018, NAAS Rating 4.97

5. Rajvir Kaur, Jasleen Kaur, Mandeep Kaur, Vishali Kalotra, Pooja Chadha, Anupam Kaur, Amarjeet Kaur. An endphytic Penicillium oxalicum isolated from Citrus limon possesses antioxidant and genoprotective potential. Journal of applied microbiology, 2019, 126, 881-893. Impact factor 3.1.

6. Rahul Datta, Amandeep kaur, Isha Saraf, Mandeep Kaur, Inder Pal Singh, Sanehdeep Kaur,Pooja Chadha. Assessment of genotoxic and biochemical effects of purified compounds of Alpinia galanga on a polyphagous lepidopteran pest. Phytoparasitica, 2020. Impact factor 1.2.

Mandeep Kaur, PhD

7. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura. BMC Microbiology, 2020. Impact factor 3.3. 8. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Prince Sharma. Effect of Aspergillus flavus on lipid peroxidation and Activity of Antioxidant Enzymes in Midgut Tissue of Spodoptera litura larvae, Archives of phytopathology and plant protection. 2020, Impact factor 0.7.

9. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur. Aspergillus flavus induced oxidative stress and immunosuppressive activity in Spodoptera litura as well as safety for mammals. 2020, Submitted.

10. Rajvir Kaur, Jasleen Kaur, Avinash Sharma, Mandeep Kaur, Vishali Kalotra, Pooja, Chadha, Anupam Kaur, Amarjeet Kaur. An endophytic Talaromyces verruculosus with alpha glucosidase inhibitory, antioxidative and genoprotective potential. 2020, Submitted.

Research Activities (Presentations in Conferences & Symposiums) 1. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur (2017). Evaluation of insecticidal potential of biological origin fungus against Spodoptera litura. International conference on “Recent advances for quality enhancement in Science and Technology”. Hans Raj Mahila Maha Vidyalaya, Jalandhar (Punjab) India. 2. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur (2017). Entomopathogenic fungus Aspergillus flavus as a potential insecticide against insect Spodoptera litura. National seminar on “Recent trends in Plant and Environmental Sciences”. Guru Nanak Dev University, Amritsar (Punjab) India.

3. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur (2018). Assessment of Aspergillus flavus induced abnormal development and cytotoxicity in Spodoptera litura. 21st Punjab science congress “scientific advances for inclusive development and environmental protection”Punjab Agriculture University, Ludhiana (Punjab) India. 4. Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Sukanya Mehra

(2018). Isolation and screening of endophytic fungi for insecticidal potential. International meets on frontier in life science. Department of biotechnology and molecular biology and biochemistry. Guru Nanak Dev University, Amritsar (Punjab) India.

5. Mandeep Kaur, Pooja Chadha (2019). Genotoxic and Cytotoxic Effects of an Endophytic Fungus Schizophyllum commune in Spodoptera litura A Possible Mechanism for its Insecticidal Activity. Joint Meeting Acadian Entomological Society Entomological Society of Canada Canadian Society for Ecology and Evolution Fredericton, NB August 18 – 21, 2019.

Mandeep Kaur, PhD

Workshops/ Certificates

1. Certificate of attending seminar on Recent Trends for Eco-restoration (2017). Department of Zoology, Guru Nanak Dev University, Amritsar(Punjab) India. 2. Workshop on GC-MS and IRGA training: A hand approach (2017). Department of Botanical and Environmental Sciences. Guru Nanak Dev University, Amritsar (Punjab) India.

3. Six day GIAN course on “Post transcriptional control of gene expression in eukaryotes”

(2017). Guru Nanak Dev University, Amritsar(Punjab) India. 4. Thirteen day GIAN course on “Chemical Catalysis in Biology” (2016). Guru Nanak Dev University, Amritsar (Punjab) India.

Research Expertise

1. Isolation of genomic DNA by organic and inorganic method, quantification of DNA using spectrophotometric method, restriction digestion. 2. Separation of immune cells, TLC, DLC, lymphocyte culture and immunoassays, use of haemocytometer.

3. Electrophoresis: Agarose gel electrophoresis, SDS-PAGE. 4. Spectroscopic techniques: used to estimate total and reducing sugars, absorption maxima of aromatic amino acids, protein estimation by Biuret, Bradford, Lowry and UV methods etc.

5. Genotoxicity testing by comet assay, cytotoxicity testing by using different fluorescent dyes under fluorescent microscope and by Scanning Electron Microscope (SEM). 6. Great skills to write a perfect manuscript for submission to various journals. 7. Practical handling:

Handling of rat and fish.

Insect culturing (culturing of Spodoptera litura).

Isolation and maintenance of culture of microorganisms such as Isolation of endophytes (fungi from plants), slide culture techniques for fungi, production and extraction of metabolites.

8. Well versed with spectroscopic and physical research techniques. 9. Expertise in Performing test.

10. Aware about the safety rules while using chemicals. Independent as well as team worker. Independently operated Instruments

Light microscope, Fluorescent microscope, SEM (Coating, sample preparation), UV-vis., Rotavapor, Laminar flow, Autoclave, Orbit shaker, BOD Incubator, LC-MS, HPLC. Computational Skills

1. Well versed with Operating Systems: Windows-Xp, Vista, Window 7 or 8, including data analysis & graphics.

Mandeep Kaur, PhD

2. Softwares: MEGA7 (Evolutionary study, Phylogenetic tree construction), CASP LAB

(Comet scoring), Stat Softwares (Multiple).

3. Data presentation: MS office (PowerPoint, Word, Excel etc), Origin 70. A brief summary of representative research accomplishment Genotoxicity evaluation of endophytes on insects and on mammals Nature- Scientific reports, 2018, 8, 4693

( Mandeep Kaur et al )

In search for ecofriendly alternatives to chemical insecticides, we conducted a study to assess the insecticidal potential of an endophytic fungus Schizophyllum commune and its mechanism of toxicity by studying genotoxic and cytotoxic effects as well as repair potential using Spodoptera litura (Fabricius) as a model. Different endophytic fungi were isolated and tested for their insecticidal potential against S. litura. Among the tested endophytic fungi maximum mortality against S. litura was exhibited by S. commune isolated from Aloe vera. Extended development, reduced adult emergence was observed in larvae fed on diet supplemented with fungal extract. In addition to it the fungus also has propensity to increase oxidative stress which leads to significantly higher DNA damage. The significantly lower frequency of living haemocytes and increased frequency of apoptotic and necrotic cells was also observed in larvae treated with fungal extract. The extent of recovery of damage caused by fungus was found to be very low indicating long term effect of treatment. Phytochemical analysis revealed the presence of various phenolics, terpenoids and protein in fungal extract. Biosafety analysis indicated the non toxic nature of extract. This is the first report showing the insecticidal potential of S. commune and the genotoxic and cytotoxic effects associated with it. References

1. Dr. Pooja Chadda, Assistant Professor,

Department of Zoology,

Guru Nanak Dev University,

Amritsar-143005, India

E-mail: adkbj1@r.postjobfree.com

2. Dr. Arvinder Kaur, Professor,

Department of Zoology,

Guru Nanak Dev University,

Amritsar-143005, India

E-mail: adkbj1@r.postjobfree.com

3. Dr. Sanehdeep Kaur, Professor,

Department of Zoology,

Mandeep Kaur, PhD

Guru Nanak Dev University,

Amritsar-143005, India

E-mail: adkbj1@r.postjobfree.com

4. Dr. Amarjeet Kaur, Assistant Professor,

Department of Microbiology,

Guru Nanak Dev University,

Amritsar-143005, India

E-mail: adkbj1@r.postjobfree.com

Declaration

I hereby declare that the information furnished above is true to the best of my knowledge and belief and that the documents in support of the information will be forwarded on request. Place : Canada Mandeep Kaur

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