molecilar biologist

SUKHVINDER KAUR (CV)

*C -***, Ist Floor, N.I.T. Faridabad, Haryana, India. 981*******. *********@**********.***

Highly motivated and innovative molecular biologist with specialization in molecular genetics, cell biology, microbiology and biochemistry. Expertise in development and execution of genetic and biochemistry-based research methods. Passionate about learning new scientific skills. Enthusiastic team player adept at providing leadership while also learning from fellow team members. Experienced in managing multiple projects simultaneously. Effective communicator and teacher that can conceptualize complex biological to audiences with varied expertise.

Skills

Molecular Biology and Biochemistry: DNA Isolation, RNA Isolation, Plasmid Isolation, PCR, RT-PCR, Agarose Gel Electrophoresis, SDS-PAGE, RFLP, Bacterial Competent Cell Preparation, Transformation, Cloning, Biochemical Assays, 5 RACE library preparation, Yeast competence cell preparation, Yeast transformation, LAMP assay.

Immunology: ELISA, Ouchterlony immunodiffusion technique, Radial immunodiffusion, Rocket Electrophoresis Sandwich dot ELISA, Leukocyte counts.

Microbiology: Methods of Culturing Aerobes and Anaerobes, Methods of Isolation and Maintenance of Pure Culture, Types and Preparation of Media, Smearing and Staining.

Primer Explorer Version 3 software (A Bioinformatics software used for primer

designing)

Bioinformatic analysis of DNA and proteins

Blast, Clustal W, Phylip, Expasy

Data mining using R and Hadoop

Work History and Education

UGC-PDF for Women Scientist -2014 onwards till date working as PDF in JMI New Delhi on project entitled “Genome profiling to study the role of single nucleotide polymorphism (SNP) in migraine”.

PhD thesis entitled “Molecular characterization of genetic determinants of arsenic (As) resistance in bacteria” submitted to Jamia Millia Islamia, New Delhi in 2008 and awarded degree in 2009.

M.Sc. Biochemistry: 1996-1999 from Punjab Agricultural University (PAU) Ludhiana with minor Biotechnology. Thesis submitted entitled “Regulation of sucrose storage in cell suspension cultures of sugarcane (Saccharum hybrid)”with 78.4%.

B.Sc. with Botany, Zoology and Chemistry: 1993-1996 from GCW Ludhiana with 75%.

Research Expertise

Experience in molecular microbiology with strong background in Plant-Microbe interaction, Antibiotic Resistant Bacteria, SNP detection and LAMP assay. Experience in widely used Computer Programing like R and Hadoop, LaTeX software, and Laboratory instruments.

Research Outcomes

During M.Sc. thesis the research was done with the objectives of delineating the role of nitrogen, phosphate, metal ions and hormones in the accumulation of sucrose in the cell suspension cultures and determining the activities of enzymes of sucrose metabolism viz. sucrose synthase, sucrose phosphate and invertase in relation to sucrose accumulation in high and low sucrose synthesizing genotypes. Sugar and protein accumulation were adversely affected in sugarcane cell cultured in the presence of 6mM carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The proteins declined by more than 80% while sugar by 60%. Late maturing sugarcane cultivar CoS767 was more susceptible to inhibition than early maturing cultivar C0J64.

During PhD objectives were undertaken to identify the E. coli strains from fresh water bodies that are resistant to arsenic heavy metal toxicity and to describe the divergence of the arsenic resistant determinants of arsenic -resistant bacteria. Here 17 strains exhibiting the highest levels of tolerance to the arsenic were selected and their nucleotide sequences for both arsC and ars A genes were determined. Further phylogenetic analysis was done to check the divergence in strains and the results were submitted in NCBI GeneBank with accession numbers. These genetic determinants will be of great help in constructing plant expression vectors which in turn will be useful to create transgenic plants with arsenic resistance properties for contaminated soils.

In PDFWM the project was aimed to study the role of single nucleotide polymorphisms (SNP) in migraine. For this after getting ethical clearance from Jamia Millia Islamia committee and ESIC Medical College, blood samples were collected from individuals suffering from migraine as well as healthy controls belonging to different age, race and sex and nine following SNPs along with their genes were selected to study the potentially present risk for migraine:-

rs1801133 and rs1801131, ch1, MTHFR gene

rs11172113, ch12, LRPI gene

rs4379368, ch7, Succinic HMG coenzyme A transferase (C7orf10) gene

rs10504861, ch8, MMP16 gene

rs2658199, ch1, PRDM16 gene

rs10166942, ch2, TRPM8 gene

rs2052129 and rs10156191, ch7, DAO gene

Genotyping was done by PCR-RFLP method. Genotypic and allelic frequencies were compared by SPSS 24 version. On comparing migraine subclasses, migraine with aura (MA) and without aura (MO) with control groups, the present study suggested that in MTHFR polymorphisms, the prevalence of 677CT genotype and T allele in C667T SNP influences susceptibility to MA (p < 0.05) but not to MO. Meanwhile, CC genotype in A1298C SNP could be a risk factor for migraine patients without aura (p < 0.05). For rs11172113 SNP, the CC genotype was higher in migraine (20 vs. 11%) and show significant difference on relating with CT genotype (p<0.05). This study was able to associate the role of rs 4379368 SNP with migraine susceptibility and suggested that genotype CT in rs4379368 SNP could be a genetic marker for migraine with aura (MA) but not with migraine without aura (MO). This study confirmed the association of PRDM16 to migraine susceptibility in MO and female subgroup at both genotypic and allelic level in the North Indian population and found that rs10166942 variant may be a potential marker for MA. Findings from this research work clearly indicated that a female patient with rs10156191T allele and in MA subgroup showed an increased risk for migraine. This study also confirmed that rs2052129T variant plays a significant role in migraine susceptibility in this population.

Personal Information

Husband Name: Mr. R.P. Singh

Nationality: Indian

Permanent Address: 3c -240, Ist Floor, N.I.T. Faridabad, Haryana, India.

Date of Birth: 10/09/1975

Marital Status: Married

Sex: female

Fluency: English, Hindi and Punjabi.

Awards Received

JRF-CSIR-NET (June-2003)

UGC-PDFWM(2014-19)

Teaching Experience

Two years teaching experience at the Postgraduate level in the department of Biotechnology in Jamia Millia islamia, New Delhi, India.

One year teaching experience at postgraduate level in the department of Biosciences in JMI during Postdoc.

Referee

Dr. Arif Ali, Professor (Rtd.), Department of Biosciences, Jamia Millia Islamia, New Delhi.

Email: ***.*******@*****.***

Dr. A. K. Pandey, Head, Department of Physiology, ESIC Medical College & Hospital, Faridabad, Haryana

Email: **********@*****.**.**.

Dr. J.S. Virdi, Professor, Department of Microbiology, University of Delhi, South Campus, New Delhi.

Email: **********@**********.***

Research Publications

Association of MTHFR gene polymorphisms with migraine in North Indian population. Sukhvinder Kaur, Arif Ali, Anil Kumar Pandey and Balkirat Singh. Neurol Sci (2018) 39; 4: 691-698. Impact factor: 2.484

(DOI: 10.1007/s10072-018-3276-7) PMID:29427165

rs2651899 variant is associated with risk for migraine without aura from North Indian Population. Kaur, S., Ali, A., Ahmad, U. et al. Mol Biol Rep (2019) 46:1 1247-55. Impact factor: 2.107

https://doi.org/10.1007/s11033-019-04593-1

Could rs 4379368 could be a genetic marker for North Indian common migraine patients with aura?: Preliminary evidence by a replication study- kaur,S., Ali A., Siahbalaei Y., Ahmad U., Pandey AK., Singh B. Neuroscience letters. 712(2019) 134482. Impact factor: 2.173

http://doi.org/10.1016/j.neulet.2019.134482.

Diversity of arsenate reductase genes (arsC Genes) from arsenic-resistant environmental isolates of E. coli. Curr Microbiol. (2009) Sep; 59 (3): 288-94. Kaur, S., Kamli, M.R. & Ali, A. Impact factor: 1.595

https://doi.org/10.1007/s00284-009-9432-9.

Role of arsenic and its resistance in nature. Can. J. Microbiol. 57: 769-74 (2011). By Kaur S, Kamli MR and Ali A. (2011) 57(10): 769-774. Impact factor: 1.550

https://doi.org/10.1139/w11-062.

Association of Diamine oxidase variants with the risk for migraine from North Indian population- kaur,S., Ali A., Siahbalaei Y., Ahmad U., Nargis F., Pandey AK., Singh B. Accepted in Metagene. Impact factor: 0.625

https://doi.org/10.1016/j.mgene.2019.100619

Role of Single Nucleotide Polymorphisms (SNPs) in Migraine-Kaur, S., Ali, A., Ahmad, U. et al. Egypt J Neurol Psychiatry Neurosurg (2019) 55: 47. Impact factor: 0.200

https://doi.org/10.1186/s41983-019-0093-8.

Conferences and workshop

Scientific program FIPS congress-2019 pre workshop and conference attended at ESIC Medical College & Hospital, Faridabad

Strength

I am highly flexible and adaptable hence can modulate myself according to the

requirements of the situations, I posses a constant learning attitude. My aim is

to work in an environment where I can utilize and enhance my skills and

knowledge to add value to the organization /institute.

Declaration

I do hereby declare that all the information furnished above is true to the best of

my knowledge.

Place: Faridabad

(Sukhvinder Kaur)

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