Cancer Research, Clinical Research, Project management

Dr. Began Gopalan

** *****’s Close, #**-***, Singapore 140021/E: ******@*****.***/M: 9011 2456.

Residential status: Singaporean PR

PROFESSIONAL SUMMARY

Highly organized, proficient and professional biologist with more than 15 years of research experience in cancer, pharmaceutical institutions and industries, co-authored 28 research papers published in various peer reviewed international scientific journals and invited speaker in various international scientific conferences. Laboratory expertise in mammalian cell culture, cell transfect ion, transduction, assay development and cell therapy for in vitro and in vivo cancer models. Leverage strong problem-solving skills in analyzing complex issues and developing effective solutions. Possess excellent collaborative, decisive, strong communication, analytical, researching, presentation, report writing skills as well as the ability to develop strategic ideas for continuous growth of the company.

CORE COMPETENCIES

Cancer research Research and Development Cell Culture Cell therapy Bio analytical skills Method development Interpersonal skills Working Relationship Trouble shooting skills Technical knowledge Creativity & Integrity Communication KEY ACHIEVEMENTS

Provided complex scientific and technical leadership in determining research priorities & the plan, design and execution of research projects.

Experienced in mammalian cell culture which includes cancer lines, primary cells and stem cells.

Successfully designed and developed cell culture and cell therapy for cancer research.

Excellent skills in cell transfection, transduction methods as well as assay development.

Demonstrated excellent knowledge of understanding cell-based, biochemical fluorescence detection methods and assays development.

Ability to work collaboratively & share cell culture as well as other scientific knowledge with cross-functional teams to reach common goals

Liaised between clients and developers on feature requests in the project work.

Strong communication, presentation, writing and team skills along with the ability to maintain professional working relationships with management, co-workers, and clients

Published 28 research papers in peer-reviewed scientific journal in the area of cancer, pharmaceutical and molecular therapy and invited speaker in various international conferences.

CAREER HISTORY

1

Self-Employed

(Freelancer)

August 2018 – to

present

Tutoring Biology, Chemistry, analytical instruments and Health care related subjects

Prepare summaries of experimental subjects and contributes to the evaluation and discussion of results

2

Lecture (Part time)

Dimensional

International College,

Singapore/Cardiff

Metropolitan

University UK

May 2017 – June 2018

Handled various health care related modules like Medical Biochemistry, Biotechnology and Laboratory cell culture system.

Experienced in sharing knowledge of cell culture, cell transfection, transduction, cell therapy and assay development.

Implemented analytical procedures and strategies.

Assessed student assignments, reports, examination papers and grade student performance

3

Self-Employed

(Freelancer)

May 2013 – April 2016

Developed and validated cell culture system.

Created and monitored data management, statistical analyses, and report writing.

Updated skills on cell therapy and assay development.

Reviewed research project and study report.

Evaluated therapeutic area and indication training for the project clinical team.

4

Research Scientist

Institute of

Bioengineering and

Nanotechnology

Singapore.

March 2006 –Dec 2012

Demonstrated ability to plans, schedules, conducts or coordinates detailed phases of the technical work in a part of a major projects and small- moderate scope projects.

Developed suitable in vitro mammalian cell culture system for cancer research.

Experienced in cell transfection, cell therapy and transduction.

Assisted in the development and validation of cell based assay system.

Experienced in creating stable cell lines for cancer and other human disease models.

Published 8 research papers in peer reviewed journals, Participated in various project team meetings and scientific conferences. 5

Post Doctoral Fellow

and Research

Investigator

Baylor College of

Medicine & MD

Anderson Cancer

Center, Houston,

University of Texas,

(USA)

June 2000 - Feb 2006

Designed and supervised the project entitled “Nanoparticle (non-viral) and viral based systemic gene therapy for Human health care.

Developed in vitro and in vivo mammalian cell culture models for therapy against cancers.

Experienced in cell transfection and cell transduction.

Experienced in cell therapy and method validation

Published 14 research papers in peer reviewed journals, Participated in various project team meetings and scientific conferences 6

Post Doctoral Fellow

Kumamoto

University,

Kumamoto (Japan)

May 1998 -April 2000

Designed and conducted the experiments using cell culture system for the therapeutic effects of compounds against cancer.

Analyzed and investigated various therapeutic compounds using diagnostic techniques.

Developed study associated monitoring tools for research project.

Published 2 research papers in peer reviewed journals, Participated in various project team meetings and scientific conferences 7

PhD

CFTRI, University of

Mysore, India

May 1998 -April 2000

Designed and conducted the experiments on the extraction and isolation of various therapeutic compounds against cancer.

Excellent knowledge in understanding recombinant protein expression.

Published 6 research papers in peer reviewed journals, Participated in various project team meetings and scientific conferences EDUCATION, FELLOWSHIP & AWARDS

Educational Credentials:

Ph.D. in Biochemistry, Central Food Technological Research Institute, University of Mysore, Karnataka (India) 1992-1998

M.Sc. in Biochemistry, PSG College of Arts and Science, Bharathiar University (India) 1989- 1991

B.Sc. in Biochemistry, PSG College of Arts and Science, Bharathiar University (India) 1986- 1989

Fellowship:

Post-doctoral fellowship (2002-2006) from M.D. Anderson cancer center (MDACC) USA for research at the M.D. Anderson Cancer center, University of Texas, Houston

Post-doctoral fellowship (2000-2002) from Baylor College of Medicine (BCM) USA for research at the Baylor College of Medicine

Post-doctoral fellowship (1998-2000) from Japan Society for the Promotion of Science (JSPS) Japan for research at the University of Kumamoto

Senior Research Fellowship (1995-1998) from the Council of Scientific and Industrial Research

(CSIR) India for doctoral research at the Central Food Technological Research Institute, University of Mysore.

Awards:

Jay and Lori Eisenberg Research Award - M.D Anderson Cancer Center, University of Texas, Houston 2005

Best Poster award at Third International Food Convention (IFCON) – Central Food Technological Research Institute, University of Mysore, India September 1993 Publications and Conferences:

Co-author of 28 papers in peer reviewed journals associated with clinical research.

Co-author of 4 patents.

Presented 18 co-authored papers at Symposia and Seminars including invited talks, oral and poster presentations.

PUBLICATIONS

1) K. Narayanan, M. Khan, B. Gopalan, J. Antony, T.Das, Y.Y.Yang, A. C. A. Wan. Sensitization of Cancer Cells via Non Viral Delivery of Apoptosis Inducing Proteins Using a Cationic Bolaamphiphile. Biotechnology, First published: 26 May 2018 https://doi.org/10.1002/biot.201800020, IMPACT FACTOR: 3.446. 2) K. Narayanan, N. Erathodiyil, B. Gopalan, S. Chong, A.C.A. Wan, and J.Y. Ying. Targeting Warburg Effect in Cancers with PEGylated Glucose. Adv. Healthcare Mater. 2016, 5, 696–701 IMPACT FACTOR 5.11.

3) B. Gopalan,

K. Narayanan, Z. Ke, T. Lu, Y. Zhang, and L. Zhuo. Therapeutic effect of a multi targeted imidazolium compound in hepatocellular carcinoma,” Biomaterials 35-201*-****-**** IMPACT FACTOR 8.402.

4) M. R. Reithofer, K.-H. Chan, A. Lakshmanan, D. H. Lam, A. Mishra, B. Gopalan, M. Joshi, S. Wang, and C. A. E. Hauser, "Ligation of Anti-Cancer Drugs to Self-Assembling Ultrashort Peptides by Click Chemistry for Localized Therapy,” Chem. Sci., 5 (2014) 625-630 IMPACT FACTOR 8.668.

5) Yao Li, Zay Yar Oo, Shu Yung Chang, Peng Huang, Kim Guan Eng, Jia Liu Zeng, Alicia J. Kaestli, Began Gopalan, karthikeyan kandasamy, Farah Tasnim and Daniele Zink. An in vitro method for the prediction of renal proximal tubular toxicity in humans,” Toxicol. Res., 2 (2013) 352-365 IMPACT FACTOR 1.969.

6) K Narayanan, K M. Schumacher, F.Tasnim, K Kandasamy, A Schumacher, M Ni, S Gao, B. Gopalan, D. Zink and J. Y. Ying. Human embryonic stem cells differentiate into functional renal proximal tubular-like cells,” Kidney International, 83(2013) 593-603 IMPACT FACTOR 8.395. 7) B. Gopalan, Z. Ke, C. Zhang, Y. Kng, N. A. Suhaimi, S. N. Riduan, Y. Zhang and L. Zhuo,

"Metal-Free Imidazolium Salts Inhibit the Growth of Hepatocellular Carcinoma in a Mouse Model," Laboratory Investigation, 91 (2011) 744-751 IMPACT FACTOR 4.857. 8) B. Gopalan, M. Shanker, A. Scott, C.D. Branch, S. Chada, R. Ramesh, "MDA-7/IL-24, A Novel Tumor Suppressor/cytokine is Ubiquitinated and Regulated by the Ubiquitin-proteasome System, and Inhibition of MDA-7/IL-24 Degradation Enhances the Antitumor Activity," Cancer Gene Ther., 15[1] (2008) 1-8 IMPACT FACTOR 3.652.

9) M. Shanker, B. Gopalan, S. Patel, D. Bocangel, S. Chada, R. Ramesh, "Vitamin E Succinate in Combination with MDA-7 Results in Enhanced Human Ovarian Tumor Cell Killing through Modulation of Extrinsic and Intrinsic Apoptotic Pathways," Cancer Lett., 254[2] (2007) 217-226 IMPACT FACTOR 6.375.

10) Y. Oida*, B. Gopalan*, R. Miyahara, P. Chiao, C. Branch, S. Chada, R. Ramesh, "Inhibition of Nuclear Factor kB Augments Antitumor Activity of Adenovirus-mediated Melanoma Differentiation–associated Gene-7 Against Lung Cancer Cells via MEKK1 Activation," Molecular Cancer Therapeutics, 6[4] (200*-****-**** (*equally contributed) IMPACT FACTOR 5.764.

11) B. Gopalan, M. Shanker, S. Chada, and R. Ramesh, "MDA-7/IL-24 Suppresses Human Ovarian Carcinoma Growth in vitro and in vivo, "Molecular Cancer, 6 (2007) 11 IMPACT FACTOR 6.204.

12) S. Inoue, M. Sankar, R. Miyahara, B. Gopalan, S. Patel, Y. Oida, C.D. Branch, A. Munshi, R.E. Meyn, M. Andreeff, F. Tanaka, A.M. Mhashilkar, S. Chada, and R. Ramesh, "MDA-7/IL-24- Based Cancer Gene Therapy: Translation from the Laboratory to the Clinic,"Current Gene Therapy, 6[1] (2006) 73-91 IMPACT FACTOR 5.318.

13) B. Gopalan, A. Litvak, Sharma S, A.M. Mhashilkar, S. Chada, and R. Ramesh, "Ad.mda-7/IL-24 Mediated Killing of Human Ovarian Cancer Cell Involves the Fas/FasL Signaling Pathway,"Cancer Res., 65-200*-****-**** IMPACT FACTOR 9.329. 14) Y. Saito, R. Miyahara, B. Gopalan, A. Litvak, S. Inoue, M. Sankar, C.D. Branch, A.M. Mhashilkar, J.A. Roth, S. Chada, and R. Ramesh, "Selective Induction of Cell Cycle Arrest and Apoptosis in Human Prostate Cancer Cells through Adenoviral Transfer of the Melanoma Differentiation Associated-7 mda-7) / nterleukin-24 (IL-24) gene,"Cancer Gene Ther., 12 (2005) 238-247 IMPACT FACTOR 3.652.

15) Y. Oida, B. Gopalan, R. Miyahara, S. Inoue, A.M. Mhashilkar, J.A. Roth, S. Chada, and R. Ramesh, "Sulindac Enhances Ad-md7/Il-24 Mediated Killing of Lung Tumor Cells,"Mol. Cancer Ther., 4 (2005) 291-304 IMPACT FACTOR 5.764.

16) I. Ito, L. Ji, F. Tanaka, Y. Saito, B. Gopalan, C.D. Branch, K. Xu, N.E. Atkinson, B.N. Bekele, L.C. Stephens, J.D. Minna, J.A. Roth, and R. Ramesh, "Liposomal Vector Mediated Delivery of the 3p FUS1 Gene Demonstrates Potent Antitumor Activity Against Human Lung Cancer in vivo,"Cancer Gene Ther., 11 (2004) 733-739 IMPACT FACTOR 3.652. 17) R. Ramesh, I. Ito, B. Gopalan, Y. Saito, A.M. Mhashilkar, and S. Chada, "Ectopic Production of MDA-7/IL-24 Inhibits Invasion and Migration of Human Lung Cancer Cells," Molecular Therapy, 9 (2004) 510-518 IMPACT FACTOR 6.938.

18) B. Gopalan, I. Ito, C.D. Branch, L.C. Stephens, N. Bekele, J.A. Roth, and R. Ramesh,

"Nanoparticle Based Systemic Gene Therapy for Lung Cancer: Molecular Mechanisms and Strategies to Suppress Lipoplex-mediated Inflammatory Response," Technolnogy in Cancer Research and Treatment, 3 (2004) 647-657 IMPACT FACTOR 2.204. 19) Y. Saito, B. Gopalan, A.M. Mhashilkar, J.A. Roth, S. Chada, L. Zumstein, and R. Ramesh,

"Adenovirus-medaited PTEN Treatment Combined with Caffeine Produces a Synergistic Therapeutic Effect in Colorectal Cancer Cells," Cancer Gene Therapy, 10 (2003) 803-813 IMPACT FACTOR 3.652.

20) I. Ito, B. Gopalan, I. Mohiuddin, T. Saeki, Y. Saito, C.D. Branch, A. Vaporciyan, L.C. Stephens, N. Yen, J.A. Roth, and R. Ramesh, "Increased Uptake of Liposomal-DNA Complexes by Lung Metastases following Intravenous Administration," Mol Ther., 7 (2003) 409-418 IMPACT FACTOR 6.938.

21) B. Gopalan, M. Goto, A. Kodama, and T. Hirose, "Response Surfaces of Total Oil Yield of Turmeric (Curcuma Longa) in Supercritical Carbon Dioxide,"Food Res Internat., 33 (2000) 341- 345 IMPACT FACTOR 3.77.

22) B. Gopalan, M. Goto, A. Kodama, and T. Hirose, "Supercritical Carbon Dioxide Extraction of Turmeric (Curcuma Longa)," J. of Agric. Food Chem., 48-200*-****-**** IMPACT FACTOR 3.154.

23) B. Gopalan, B. Manohar, K.U. Sankar, and A.G.A. Rao, "Response Surface of Solubility of Total Lipid of Crude Soylecithin in Supercritical Carbon Dioxide," Eur Food Res Technol., 210[3]

(2000) 209-212 IMPACT FACTOR 1.664.

24) B. Gopalan, E. Sudharshan, K.U. Sankar, and A.G.A. Rao, "Interaction of Curcumin with Phosphatidylcholine -A Spectrofluorometric Study," J. Agric. Food Chem., 47 (1999) 4992 – 4997 IMPACT FACTOR 3.154.

25) B. Gopalan, E. Sudharshan, and A.G.A. Rao, "Change in the Positional Specificity of Lipoxygenase-1 due to Insertion of Fatty Acids into Phosphatidylcholine Deoxycholate Mixed Micelles," Biochemistry, 38 (1999) 139**-***** IMPACT FACTOR 2.938. 26) B. Gopalan, E. Sudharshan, and A.G.A. Rao, "Inhibition of Lipoxygenase 1 by Phosphatidylcholine Micelles -Bound Curcumin," Lipids, 33-199*-****-**** IMPACT FACTOR 1.934.

27) B. Gopalan, B. Manohar, and K.U. Sankar, "Enrichment of Phosphatidyl Choline by Alcohol Fractionation of Carbon dioxide De-oiled Lecithin," Z Lebensm Unters Forsch A,

(International Journal of Food Research and Technology), 205 (1997) 360-363 IMPACT FACTOR 1.08.

28) B. Manohar, B. Gopalan, and K.U. Sankar, "Response Surface Methodology Applied to Fractionation of De-oiled Soyalecithin Obtained by Supercritical Carbon Dioxide Extraction," Lebensm.-Wissu.-Technol., 28 (1995) 218-221 IMPACT FACTOR 4.03. PATENTS

1. L. Zhuo, C. Zhang, Y. Zhang, J. Y. Ying, B. Gopalan, Z. Ke, Z. Ding, "Imidazolium Salts as Anti- Oxidative, Anti-Inflammatory and Anti-Fibrotic Agents," Singapore Patent Granted on August 15, 2011

2. L. Zhuo, C. Zhang, Y. Zhang, J.Y. Ying, B. Gopalan, Z. Ke, Z. Ding, "Method For Treating Fibrosis And Cancer With Imidazolium And Imidazolinium Compounds," Singapore Patent Granted, August 15, 2011.

3. L. Zhuo, C. Zhang, Y. Zhang, J.Y. Ying, B. Gopalan, Z. Ke, Z. Ding, "Method For Treating Fibrosis And Cancer With Imidazolium And Imidazolinium Compounds," US Patent Application No. 12/865,668S .

4. KU. Sankar, G.Began and B. manohar. A process for preparing purified phosphatidyl choline from plant lecithin. Ref. No. FT/TT/053(109)/MS/94.

ACADEMIC CONFERENCE PRESENTATIONS

1. F.R. Strebel, W. Deng, E. Rachal, D.S. Loose, J.L. Alcorn, B. Gopalan, R. Ramesh, R.W. Rowe, and J.M.C. Bull, "Transgene Expression in vivo: Effects of Species Difference and Intravenous Infusion Rate of Cationic Liposome DNA Complex," 96th AACR Annual Meeting, 16-20 April 2005, Anaheim, California, USA

2. B. Gopalan, M. Shanker, S. Chada, and R. Ramesh, "Adenovirus-mediated Transfer of the mda-7 Gene Suppresses Human Ovarian Cancer Growth in vitro and in vivo," 96th AACR Annual Meeting, 16-20 April 2005, Anaheim, California, USA 3. B. Gopalan, C.D. Branch, C.L. Stephens, J.A. Roth, and R. Ramesh, "A Novel Strategy for Suppressing Toxicity-mediated by Systemic Delivery of DNA- Nanoparticles," 96th AACR Annual Meeting, 16-20 April 2005, Anaheim, California, USA 4. Y. Saito, B. Gopalan, A.M. Mhashilkar, J.A. Roth, S. Chada, L. Zumstein, and R. Ramesh,

"Adenovirus-medaited PTEN Treatment Combined with Caffeine Produces a Synergistic Therapeutic Effect in Colorectal Cancer Cells," 95th AACR Annual Meeting, 27-31 March 2004, Orlando, Florida, USA

5. Y. Oida, B. Gopalan, S. Inoue, R. Miyahara, A.M. Mashilkar, B.N. Bekele, J.A. Roth, S. Chada, and R. Ramesh, "mda-7/IL-24 in combination with Sulindac Induces a Synergistic Therapeutic Effect in Human Lung Cancer Cells," 95th AACR Annual Meeting, 27-31 March 2004, Orlando, Florida, USA

6. B. Gopalan, A. Litvak, S. Sharma, M. Sankar, B.N. Bekele, C. Branch, A.M. Mhashilkar, J.A. Roth, S. Chada, and R. Ramesh, "Ad.mda-7/IL-24 Mediated Killing of Human Ovarian Cancer Cell Involve Fas/FasL Signaling Pathway," 95th AACR Annual Meeting, 27-31 March 2004, Orlando, Florida, USA

7. R. Ramesh, I. Ito, B. Gopalan, Y. Saito, A.M. Mhashilkar, and S. Chada, "Ectopic Production of MDA-7/IL-24 Inhibits Invasion and Migration of Human Lung Cancer Cells," 12th International Conferenceon Cancer Gene Therapy, 11-13 December 2003, San Diego, USA 8. Y. Oida, B. Gopalan, S. Banerjee, R. Miyahara, A.M. Mashilkar, P. Chiao, J.A. Roth, S. Chada, and R. Ramesh, "Supression of Adenovirus-mediated mda-7/IL-24 Activation of NFkB Induces a Synergistic Therapeutic Effect in Human Lung Cancer Cells," 6th Annual American Society of Gene Therapy Meeting, 4-8 June 2003, Washington DC, USA 9. B. Gopalan, Y. Saito, C. Branch, A.M. Mhashilkar, J.A. Roth, S. Chada, and R. Ramesh,

"Adenovirus-Mediated mda-7/IL-24 Gene Transfer Induces Cell Cycle Arrest and Apoptosis in Human Ovarian Cancer Cells," 94th AACR Annual Meeting, 5-9 July 2003, Washington, USA 10. B. Gopalan, L. Ji, I. Ito, Y. Saito, C. Branch, K. Xu, L.C. Stephens, J.D. Minna, J.A. Roth, and R. Ramesh, "The Anti-Inflammatory Drug Naproxen Protects Mice from Lipoplex-Mediated Toxicity," 94th AACR Annual Meeting, 5-9 July 2003, Washington, USA 11. B. Gopalan, L. Ji, I. Ito, Y. Saito, C. Branch, K. Xu, L.C. Stephens, J.D. Minna, J.A. Roth, and R. Ramesh, "The Anti-Inflammatory Drug Naproxen Protects Mice from Lipoplex-Mediated Toxicity," 2nd Trainee Recognition Day, 25 April 2003, M.D. Anderson Cancer Center, Houston, TX, USA

12. B. Gopalan, and N.S. Templeton, "Gene Targeting via Folate and CD13 Receptors," 3rd Annual CAGT Retreat, 15-16 November 2001, TX, USA

13. B. Gopalan, M. Goto, A.A. Kodamand, and T. Hirose, "Fractionation of Turmeric Oil From Turmeric using Supercritical Chromatography," 65th CE conference, 26-30 March 2000, Tokyo, Japan

14. B. Gopalan, M. Goto, A.A. Kodamand, and T. Hirose, "Extraction of Turmeric and its Chracterisation," 64th CE Conference, 25-28 March 1999, Nagoya, Japan 15. B. Gopalan, E. Sudharshan, and A.G.A. Rao, "Fatty Acid Inserted in Phosphatidyl Choline Micelles Alters the Regioselectivity of Lipoxygenase 1.," 66th Annual Meeting of the Socitey of Bilogical Chemists, 22-24 December 1997, Vishakapatnam, India 16. B. Gopalan, E. Sudharshan, and A.G.A. Rao, "Phosphatidyl Choline Encapsulated Spice Principles: Potentiation of Lipoxygenase Inhibitory Activity," 65th Annual Meeting of the Socitey of Bilogical Chemist, 19-23 November 1996, Bangalore, India 17. B. Gopalan, K.U. Sankar, and B. Manohar, "Enrichment of Phosphatidyl Choline by Alcohol Fractionation of Carbon Dioxide," International Seminar on Challenges Facing Fats, Oleochemicals and Surfactents in the 21st Century and Expo, 19-22 December 1995, Bombay, India

18. B. Gopalan, K. Udayasankar, and T. Rao, "Extraction of Soya Lecithin Using Supercritical Carbon Dioxide," 3rd International Food Convention (IFCON), 7-12 September 1993, C.F.T.R.I., Mysore, India (Best Paper Award).

RERFERENCES:

Available upon request

Contact this candidate