RNA Biology, Biomedical science, cardiac diseases

Curriculum Vitae Chaitali Misra

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CHAITALI MISRA, PhD

Department of Biochemistry ********.*****@*****.*** University of Illinois, Urbana-Champaign ********@********.*** 461 Medical Sciences Building, Urbana, IL-61801 Phone: 217-***-**** EDUCATION AND RESEARCH EXPERIENCE

Research Scientist, University of Illinois, Urbana-Champaign 2019-present

• Awarded funding from Department of Defense (DoD) PRMRP Discovery Award on the “Role of Neuron-Specific Giant Ankyrins Isoform in Developing Cardiac Arrhythmias for Myotonic Dystrophy Type 1 (DM1) Patient”.

• Developed human DM1 patient derived iPSC cell-based disease modeling and transgenic animal models to understand disease mechanism and develop therapeutic approaches.

• Determining the genome-wide differences in RNA binding activities of RBFOX2 isoforms to identify the defects promoting cardiac pathogenesis in DM1. Postdoctoral Research Associate, University of Illinois, Urbana-Champaign 2014-2019

(PI: Auinash Kalsotra, PhD)

• Uncovered the role of non-muscle isoform overexpression of RNA-Binding-Protein Rbfox2 in Myotonic Dystrophy patient heart in developing cardiac arrhythmias.

• Developed two Rbfox2 isoform specific mice model using CRISPR CAS9.

• Awarded funding from American Heart Association Postdoctoral fellowship.

• Published first author publication in Dev Cell, Misra et al., 2020

• Published a book chapter on “RNA metabolism deregulation in microsatellite expansion diseases”

(published by Springer).

Postdoctoral Research Fellow, Nationwide Children’s Hospital, OSU 2009-2012

(PI: Vidu Garg, MD)

• Identified the role of a Gata4 point mutation found in a human family with congenital heart disease using knock-in mutant mouse model

• Resulted in 2 first author publications (Plos Genetics, Human Molecular Genetics), and one coauthor publication (human mutation).

• Wrote an editorial article for Nature Medicine on novel genetic etiology on ventricular non- compaction cardiomyopathy with Dr. Vidu Garg

Graduate Student Researcher, Indian Institute of Chemical Biology, India 2003-2009

(PI: Susanta Roychoudhury, PhD)

• Thesis title “Analysis of polymorphic variants of wild type and mutant p53 gene in Head and Neck cancer”.

• Involved in construction of The Indian Genome Variation database (IGVdb).

• Resulted in several coauthor and first author publications (Molecular carcinogenesis, International journal of cancer, Journal of clinical pathology). Curriculum Vitae Chaitali Misra

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Master’s in science in Biotechnology, Visva-Bharati University, India 1998-2000 Bachelor of Science in Botany(major), Zoology, Chemistry, Calcutta University, 1995-1998 Honors, Fellowships and Awards

DOD (PRMRP) Discovery Award 2019

Excellent oral presentation award at the Rustbelt RNA Meeting, Columbus, Ohio. 2018 AHA Postdoctoral Fellowship 2016

Oral presentation award at the "UIUC-COM Research Day" symposium 2016 Associate Faculty Member, Faculty of 1000 2014

Keystone Symposia Scholarship award, National Heart, Lung, and Blood Institute, USA 2012 Service star, Nationwide Children’s Hospital, USA 2010 CSIR-UGC-NET Predoctoral Fellowship, CSIR, India 2003 Qualified in GATE (Graduate Aptitude Test in Engineering-2002) Examination and scored 93.23 percentile, IIT, India 2002

Stood first in M.Sc. Examination in Biotechnology, Visva-Bharati University 2000 PUBLICATIONS

1. Chaitali Misra, Sushant Bangru, Darren J. Parker, Sara Koenig, Ellen Lubbers, Jamila Hedhli, Thomas A. Cooper, Wawrzyniec L. Dobrucki, Peter Mohler and Auinash Kalsotra “Aberrant Expression of a Non-muscle RBFOX2 Isoform Triggers Cardiac Conduction Defects in Myotonic Dystrophy”. Dev Cell. 2020 Mar 23;52(6):748-763.e6. doi: 10.1016/j.devcel.2020.01.037. Epub 2020 Feb 27. (PMID: 32109384). 2. Misra C., Lin F., Kalsotra A. (2018) Deregulation of RNA Metabolism in Microsatellite Expansion Diseases. In: Sattler R., Donnelly C. (eds) RNA Metabolism in Neurodegenerative Diseases. Advances in Neurobiology, vol 20. Springer, Nature.

3. Chaitali MisraƗ, Sheng-Wei Chang Ɨ, Madhumita Basu, Nianyuan Huang and Vidu Garg (2014). “Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte proliferation and atrioventricular septation”. Human Molecular Genetics, 2014 Oct 1; 23(19):5025-35 (Ɨ Authors contributed equally), doi:10.1093/hmg/ddu215 (PMID:24858909).

4. Chaitali Misra, Vidu Garg (2013). “Compacting the heart with Notch”. Nature Medicine (News & Views). February 6 (19) 133-134, doi:10.1038/nm.3071 (PMID: 23389606). 5. Chaitali Misra, Nita Sachan, Caryn Rothrock McNally, Sara N. Koenig, Caryn Rothrock McNally, Haley A. Nichols, Anuradha Guggilam, Pamela A. Lucchesi, William T. Pu, Deepak Srivastava and Vidu Garg (2012).

“Human CHD Causing Mutation GATA4 G296S Mutation Displays Cardiomyocyte Proliferation Deficits in vivo”. PLoS Genetics, 8(5): e1002690. (PMID: 22589735). 6. Chaitali Misra, Mousumi Majumder, Swati Bajaj, Saurabh Ghosh, Bidyut Roy and Susanta Roychoudhury

(2009). “Polymorphisms at p53, p73 and MDM2 loci modulate the risk of Tobacco Associated Leukoplakia and Oral Cancer”. Molecular Carcinogenesis, 48(9):790-800 (PMID: 19204927). 7. “EGLN1 involvement in high-altitude adaptation revealed through genetic analysis of extreme constitution types defined in Ayurveda” Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18961-6. Epub 2010 Oct 18 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 20956315).

8. Sheng-Wei Chang, Mona Mislankar, Chaitali Misra, Nianyuan Huang, Daniel G.DaJusta, Steven M. Harrison, Kim L. McBride, Linda A. Baker and Vidu Garg (2013). “Genetic abnormalities in FOXP1 are associated with congenital heart defects”. Human Mutation 2013, June 13 (PMID: 23766104). 9. “Complex Patterns of Genomic Admixture within Southern Africa” PLoS Genet. 2013;9(3):e1003309 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 23516368).

10. S Mitra, C.Misra, R K Singh, C K Panda, S roychoudhury( 2005). “Association of specific genotype and haplotype of p53 gene with cervical cancer in India”. J Clin Pathol. 2005 58:26-31 (PMID: 15623478). 11. “Differential serum cytokine levels are associated with cytokine gene polymorphisms in north Indians with active pulmonary tuberculosis” Infect Genet Evol. 2011 Jul;11(5):1015-22 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 21463712). Curriculum Vitae Chaitali Misra

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12. Sayan Mitra,Nilabja Sikdar, Chaitali Misra, Shalini Gupta, Ranjan Rashmi Paul, Bidyut roy, Chinmay Kumar Panda and Susanta Roychoudhury. “Risk assessment of p53 genotypes and haplotypes in tobacco-associated leukoplakia and oral cancer patients from Eastern India”. Int. J. Cancer 2005 117:786-93 (PMID: 15981211). 13. Mitra S, Banerjee S, Misra C, Singh RK, RoyA, Sengupta A, Panda CK, Roychoudhury S. “Studies on interplay between Human Papilloma Virus Infection and p53 gene alterations in head and neck squamous cell carcinoma of an Indian patient population”. J Clin Pathol. 2007 60:1040-7 (PMID:17079356). 14. “Presence of strong association of the major histocompatibility complex (MHC) class I allele HLA-A*26:01 with idiopathic hypoparathyroidism.” J Clin Endocrinol Metab. 2012 Sep;97(9):E1820-4 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article)

(PMID:22723329).

15. “Utilizing linkage disequilibrium information from Indian Genome Variation Database for mapping mutations: SCA12 case study” J Genet. 2009 Apr;88(1):55-60 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 19417544). 16. “Single nucleotide polymorphisms in homocysteine metabolism pathway genes: association of CHDH A119C and MTHFR C677T with hyperhomocysteinemia” Circ Cardiovasc Genet. 2009 Dec;2(6):599-606 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 20031640).

17. “Mapping human genetic diversity in Asia” Science. 2009 Dec 11;326(5959) (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 20007900). 18. “Genetic landscape of people of India: A canvas for disease gene exploration. Indian Genome Variation Consortium.” Journal of Genetics, 2008 87: 3-20 (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 18560169). 19. “The Indian Genome Variation database (IGVdb): a project overview. Indian Genome Variation Consortium” Human Genetics 2005. (I am one of the participants of the Indian Genome Variation Consortium and listed as a contributor to this article) (PMID: 16133172).

Manuscript in preparation:

1. “Dissecting the role of non-muscle RBFOX2 isoform upregulation in DM1 cardiac pathogenesis” Chaitali Misra, Ullas Valiya Chembazhi, Sarah N. Matatov, Jamila Hedhli, Wawrzyniec L. Dobrucki and Auinash Kalsotra

2. “Poly(A) Binding Proteins Tune Translation in the Heart” Joe Seimetz, Sandip Chorghade, Bo Zhang, Chaitali Misra, Stefan Bresson, Nicholas Conrad, Auinash Kalsotra. Invited and Platform Presentations

Molecular and Cellular Biology Retreat, UIUC 2019

RNA Rustbelt Meeting, Columbus, OH 2018

The International Myotonic Dystrophy Consortium (IDMC-11), San Francisco, CA 2017 Institute of Post-Graduate Medical Education and Research, Kolkata, India 2017 Weinstein Cardiovascular Development and Regeneration, Duke University, NC 2016 UIUC-College of Medicine Research Day" symposium, UIUC, IL 2016 National Institute of Immunology, Delhi, India 2012 Ad hoc Reviewer for Journals: Scientific Reports, Circulation Journal, PLOS One. Certificates and Workshops

Confocal Microscope Training from UIUC, USA 2016

Certificate of completion of Data Science in Python from IBM 2016 Curriculum Vitae Chaitali Misra

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Certificate in Foundations of Teaching (University of Illinois, Urbana-Champaign) 2019 Workshops:

Teaching with Confidence 2019

Improving our testing and grading 2019

Creating a Promising Syllabus 2019

Student motivation and Activities 2019

Using Informal Early Feedback 2019

Research Support.

1) Title: Functional consequences of Rbfox2 splice isoform switch in Myotonic Dystrophy Type1 Funding Source: MWA Winter 2016 Postdoctoral Fellowship AHA ($110,000) Dates of proposed project: 07/01/16 – 06/30/18

The major goals of this project were to uncover a novel role of Rbfox2 in Myotonic Dystrophy type1 cardiac pathogenesis and to understand the tissue specific role of alternate splicing transitions during development.

2) Title: Role of Neuron-Specific Giant Ankyrins Isoform in Developing Cardiac Arrhythmia for Myotonic Dystrophy Type 1 Patient

Funding Source: Department of Defense Peer Reviewed Medical Research Program (PRMRP) Discovery Award ($317,000)

Date of Proposed project: 01/01/2020 – 12/31/2021

The major goals of this project are to investigate the underlying mechanisms of mis-splicing for large Ankyrin exons and their pathological roles in triggering cardiac arrhythmias in DM1 patients. REFEREE CONTACT INFORMATION

1. Auinash Kalsotra, PhD

Associate Professor of Biochemistry

University of Illinois, Urbana-Champaign,

506 South Mathews Ave.,

Urbana, IL 61801

Office: 217-***-****

email: ********@********.***

2. Wawrzyniec Dobrucki, PhD

Associate Professor in the Department of Bioengineering University of Illinois, Urbana-Champaign,

4261 Beckman Institute

405 North Mathews Avenue

Urbana, IL 61801

Office: 217-***-****

email: ********@********.***

3. Vidu Garg, MD

Curriculum Vitae Chaitali Misra

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Director, Center for Cardiovascular Research

The Research Institute at Nationwide Children's Hospital Research Building III

Columbus, OH 43205

email: ****.****@*******************.***

4. Stephanie Ceman, PhD

Professor of Cell and Developmental Biology

University of Illinois, Urbana-Champaign,

23 Medical Sciences Building

Urbana, IL 61801

Office: 217-***-****

email: ******@********.***

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