Food Safety Health
Resume:
Dr. Nirupma Ved
adjz2i@r.postjobfree.com
C/o DR Sharma H2 Surendra Garden colony opp baghsavaniya Police station Bhopal MP Career Biography
I hold a PhD and also a post-graduate degree in Biotechnology from Jiwaji University, India. During my PhD program I worked on understanding the molecular epidemiology and transmission dynamics of Group A rotavirus in neonates from different species. I have more than a decade of biomedical research experience working in different capacities as Scientist B
(on contractual basis), and Senior Research Fellow in research laboratories of reputed institutes in India. I have the experience of working on Avian influenza in biocontainment suite (BSL3+ laboratory) in a high security laboratory. I have also co-authored several research articles, technical articles, conference proceedings, book chapters and NCBI GenBank submissions. Through my journey of research career and quest for scientific knowledge, I have gained technical expertise in various domains of modern biomedical scientific research.
Research Experience
PhD Research: “Molecular Characterization and Genotyping of Animal and Human Rotaviruses Isolates from Northern India”. In my research program I have worked on molecular epidemiology of circulating rotavirus A strains from neonates of different species including children and understanding the transmission dynamics. The work is significant breakthrough in public health perspective and prospect of developing of a rotavirus vaccine candidate.
MSc Research: “Cloning of alpha inhibin gene in a suitable cloning vector”. This was my first exposure to modern biotechnology and molecular biology techniques which inspired me to pursue a research career in biotechnology.
Scientist B (on contractual) at King’s George Medical University, Lucknow (U.P.), India. Senior research fellow at ICAR-National Institute of High security Animal Disease Laboratory, Bhopal, India- I worked as part of the technical team working on Avian virus surveillance from domestic and wild birds all over India as the lab is the only OIE referral lab of Avian influenza in India. I worked in BSL3+ suite, handling suspected clinical samples, processing them for nucleic acid extraction for confirmatory diagnosis by molecular methods. I also screened the samples by Haemagglutination test, Haemagglutination inhibition test. I passaged the positive samples in embryonated eggs and maintained the repository. Senior Research Fellow at Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, India- I was working in a NAIP (World Bank funded) project on identification and characterization of Toll like receptors (TLRs) in yak, mithun and hill cattle. We were the first research group to identify and characterize the TLRs from these unique species. We also worked on TLR transcript regulation in viral, bacterial and parasitic disease model.
Work Experience
Designation Institute Responsibilities
(SRF)
Study coordinator
AIIMS Bhopal
(M.P.)
30.09.2020 Working on “A Randomized,
Double blind Clinical Trial for the
Development of Mycobacterium w
for COVID19” Laboratory
Diagnostics test, Case Report form
and Inform consent form filling
Patient recruitment, Follow-ups of
patients, maintain documentation
Scientist
contractual)
B (on King’s George
Medical
University,
Lucknow
(U.P.), India
10.12.2018-
28-02-2019
Research on “Strengthening
laboratory surveillance for
pneumococcal meningitis in India to
understand the impact of
pneumococcal conjugate vaccine
(PCV) rollout”.
Senior
Fellow
Research ICAR-National
Institute of
High security
Animal
Disease
Laboratory,
Bhopal, India
20.05.2013-
07.07.2014
Research on Avian influenza
surveillance network project.
Assisted in conducted Laboratory
Experiments to ASEAN countries
Trainnes, Report writing.
Senior
Fellow
Research ICAR-Indian
Veterinary
Research
Institute,
Mukteswar,
India
16.12.2008-
18.06.2012
Research on NAIP (World Bank)
funded project on identification of
TLRs in yak, mithun and also on
TLR transcript regulation in viral,
bacterial and parasitic disease
models.
Lecturer Subhash
Chandra Bose
College,
Gwalior, India
15.09.2003-
15.12.2006
Teaching
postgraduate
students
undergraduate and
Biotechnology
Academics
Qualification University Percentage
Year
PhD
(Biotechnology)
Jiwaji University, Gwalior, M.P. UGC 2009 2016
MSc. (Biotechnology) Jiwaji University, Gwalior, M.P. 68% 2003 B.Sc.
(Life sciences )
Bundelkhand University, Jhansi,
U.P.
58% 2001
Intermediate(12
th
) UP Board Allahabad 50% 1998
High School(10
th
) UP Board Allahabad 60% 1996
Special Attainments
Dr S. P. Singh Best Research Paper Award for 2019 through “Indian Association of Veterinary Public Health Specialist for publication in Journal of Veterinary Public Health entitled “G and P typing of Animal Rotaviruses from Northern and Central India” (part of PhD research).
Technical Expertise
Molecular Biology: Nucleic Acid Extraction from biomedical samples (whole blood and PBMCs, tissues, cell culture harvest supernatant, faecal samples), PCR and RT-PCR, real- time PCR, Gene Cloning, Mutagenesis, Sanger Sequencing. Immunological &Proteomics: Recombinant protein expression in heterologous system, SDS-PAGE, Immuno-blot, ELISA.
Virological: Virus propagation in cell culture and embryonated eggs, Virus titration, Virus Neutralization Test, Haemagglutination test, Haemagglutination inhibition test. Bioinformatics: Sequence retrieval and alignment, Software suites like DNAStar, MEGA7.0, BioEdit, Clustal, Primer Designing.
Publications
Research papers (12)
Citations-82, hindex-3
Nirupama Vaid, Shrivastav B. R, Malik Yashpal, Sharma Kuldeep, Yadav Naveen and S. Chakravarti Soumendu. G and P typing of Animal Rotaviruses from Northern and Central India. 2016, Journal of Veterinary Public Health. 14(I):1-7). (Impact NAAS 4.44).
C. Tosh, S Nagarajan, H. V. Murugkar, M. Kumar, M. Pandey, D. K. Jolhe, S. Tripathi, S. Shukla, NVaid, A. Mishra, R. C. Ghosh, D. D. Kulkarni. Emergence of M2Val27Ala mutation associated with amantadine-resistance in clade 2.3.2.1 H5N1 highly pathogenic avian influenza virus in India. 2014, ActaVirologica. 58: 389 – 392.
(Impact (0.636).
Y.P.S. Malik, V.Rawat, K. Sharma, N. Kumar, N. Vaid, R. Rakholia, B. Ganesh. Molecular evidence of Multiple Viral Infections in an Infant Hospitalized with Acute Gastroenteritis in India. 2014, Journal of Pure and Applied Microbiology, 733- 737. (Impact (1.48).
Y.P.S. Malik, K. Sharma, N. Kumar, A. A. Haq, N.Vaid. Determination of bovine rotavirus genotypes (G and P) circulating in Uttarakhand (2010–2012) by polymerase chain reaction. 2014, Indian Journal of Animal Sciences. 84 (2): 42– 50.(Impact (0.239).
Y.P.S. Malik, K. Sharma, N. Vaid, MunishBatra, Minakshi Prasad, Gaya Prasad, Baldev R. Gulati and Kiran N. Bhilegaonkar. Designing and validation of new primers for efficient genotyping of animal G3 rotaviruses. 2013, Veterinarski Arhiv. 83
(6), 611-622. (Impact 0.430).
Y.P.S. Malik, K. Sharma, N.Vaid, S. Chakravarti, K.M. Chandrashekar, Basera SS, R. Singh, M. Prasad, G. Prasad, B.R. Gulati, K.N. Bhilegaonkar, A.B. Pandey. Frequency of group A rotavirus mixed G and P genotypes in bovines: Predominance of G3 genotype and its emergence in combination with G8/G10 types. 2012, Journal of Veterinary Sciences. 13(3), 271-278. (Impact (1.561).
Y.P.S. Malik, D. Singh, K.M. Chandrasekhar, S. Shukla, K. Sharma, N.Vaid, S Chakravarti. Occurrence of dual infection of peste-des petits-ruminants and goat pox in indigenous goats of central India, phylogenetically relatedness of PPR virus with Tibet and Chinese strains. 2011, Transboundary and Emerging Diseases, 58(3):268-73).
(Impact 4.188).
Y.P.S. Malik, S. Chakravarti, K. Sharma, N. Vaid, K.K. Rajak, V. Balamurgan, S. Biswas, B. Mondal, R.S. Kataria and R.K. Singh. Genomic analyses of Toll-like receptor 4 and 7 exons of Bosindicus from temperate sub-Himalayan region of India. 2011, Asian Australasian Journal of Animal Sciences. 1019-1025. (Impact 1.664).
Y.P.S. Malik, K.M. Chandrashekar, K. Sharma, AdilHaq, N.Vaid, S. Chakravarti, M. Batra, A.B Pandey. Picobirnavirus detection in buffalo calves suffering from acute diarrhoea. 2011,Tropical Animal Health Production,43: 1475-78. (Impact 1.333 ).
S. Chakravarti, S. Biswas, J.L. Mohapatra, K. Sharma, N. Vaid, K.K. Rajak, R.K. Singh, B. Mondal, Y.P.S. Malik. Sequence, Phylogenetic and Homology Analysis of Toll-like Receptor Gene TLR7 in Bosindicus from High Altitude Areas of Uttarakhand State. 2014, Agricultural Research, 3(I):92-96. (Impact 0.840).
S. S. Basera, R. Singh, N.Vaid, K. Sharma, S. Chakravarti and Y.P.S. Malik. Detection of Rotavirus infection in bovine calves by RNA-PAGE and RT-PCR. 2010, Indian Journal of Virology 21(2): 144-147. (Impact 0.999).
Y.P.S. Malik, S. Chakravarti, Kuldeep Sharma, N.Vaid, A. B. Pandey. Genetic characterization and phylogenetic analysis of Innate immune - Toll like receptor 4
(TLR4) of Bos frontalis (Mithun) from North East Region, India. 2010, Journal of Immunology and Immunopathology, 973-9149). (Impact NAAS 9.55). Technical/Popular Article (1)
S Chakarvarti, N. Vaid, K Sharma, SK Biswas, YPS Malik, B Mondal and V Bhanuprakash (2009). Amplification and cloning of full length Toll-like receptor 7 gene of Bosindicus from hilly terrains of Kumaon region, Uttarakhand. IVRI News Letter. 31(2): 3-4; July-December, 2009.
Book chapters (5)
Malik Y.P.S., Kumar N, Sharma K,N.Vaid. (2013). Animal Rotaviruses. In: Trends in Diagnosis of Animal Viral Diseases. EdsYashpal S. Malik, AB Pandey and JM Kataria, Published from Division of Virology, IVRI Mukteswar 263138 (Uttarakhand), pp- 19- 36.
Y.P.S. Malik, Kumar N, Sharma K,N.Vaid. (2013). Nucleic Acid Dependent Techniques. In: Trends in Diagnosis of Animal Viral Diseases. EdsYashpal S. Malik, AB Pandey and JM Kataria, Published from Division of Virology, IVRI Mukteswar 263138
(Uttarakhand), pp- 205-220.
Y.P.S. Malik, K Sharma, N. Yadav, N. Vaid, Amit Kumar. Approaches in the Diagnosis of Rotavirus Infection, Indian Veterinary Research Institute Mukteswar, Nainital
(Uttarakhand).
Malik Y.P.S, Chakravarti S, Sharma K, N. Vaid,A.B.Pandey (2011). Toll like innate immune receptors: Potential Clinical Applications. In: ICAR sponsored 3 day workshop under NAIP project (Component 4) entitled “Toll-like receptors in Farm animals and Applications in Evolutionary Lineages in Disease Resistance” on “Real-time PCR and SNP analysis” from 2-4th Feb,2011 at Dept. of Animal Biotechnology, Madras Veterinary College, Tanuvas, Chennai.
Chakravarti S, Shrivastava S, Malik YPS, N. Vaid, Sharma K, Haq AA and Pandey AB
(2010). TLR agonsits: Novel prospective for developing smarter vaccines. In: Applied Aspects of Biotechnology, Eds. Shrivastava S, Sonal and Kumar S, Indian Veterinary Research Institute, Izatnagar 243122 (UP), India, pp 229-237 Conference abstracts (14)
C. Tosh, S. Nagarajan, H.V. Murugkar, M. Kumar, S. Shukla, S. Tripathi, N.Vaid, A. Mishra and D.D. Kulkarni. Isolation and genetic characterisation of avain influenza (sub- type H5N1) viruses Isolated from chicken, duck and crow in Odisha, 2012 and 2014 at 2nd Annual conference and National Symposium on “Integrating poultry Health and Food Safety” AAHP-2014, 7-8 Nov. 2014, Pune, India.
Z. NongothungEzung, R. Singh K. Sharma, N. Kumar, A. Kumar N Vaid and Yashpal S. Malik. Molecular typing of the structural genes of bovine and human group A Rotavirus at Virocon 2012 Indian Veterinary Research institute Muktewar campus from Nov 8-10, 2012.
N. Kumar, K. Sharma, A. Kumar Sharma, N.Vaid, S.Sircar and Y.P.S. Malik. Expression and purification of polyhistidine-tagged truncated NSP-4 protein of rotavirus E2 genotype in prokaryotic expression system at Virocon 2012 Indian Veterinary Research Institute Muktewar campus from Nov 8-10, 2012.
A.A.Haq, Y.P.S. Malik, K. Sharma, N.Vaid, S. Chakarverti. Development and evaluation of real time PCR assay for rapid diagnosis of bovine group A rotaviruses at Virocon 2012 Indian Veterinary Research Institute Muktewar campus from Nov 8-10, 2012.
A.A.Haq, Y.P.S. Malik, K Sharma, N. Vaid,S. Chakarverti. Determination of P and G genotypes in bovine rotaviruses: A need to update the genotyping primers at Virocon 2012 Indian Veterinary Research Institute, Muktewar campus from Nov 8-10, 2012.
A.K. Sharma, K. Sharma, N. Kumar, N. Vaid, Y.P.S. Malik. Bovine Picobirnaviruses: Detection, phylogenetic analysis and development of RdRp gene based RT-PCR diagnostic assay at Virocon 2012 Indian Veterinary Research Institute Muktewar campus from Nov 8-10, 2012.
N. Vaid*, Y.P.S. Malik, S. Chakravarti, K. Sharma, A. Sen, G. Venkatesan, K.N. Bhilegaonkar, A.B. Pandey. Development of rapid nucleodiagnostic assay for detection for rotaviruses: Assessment of clinical significance in farm animals. In: X Agriculture Science Congress on “Soil, Plant and Animal health for Enhanced and sustained Agricultural Productivity” held at National Bureau of Fish Genetic Resources, Lucknow from Feb10-12,2011(Poster presentation).
Y.S.P. Malik, K. Sharma, N.Vaid, S. Chakravarti, N. Kumar, A.B. Pandey. Development and Evaluation of real-time Multiplex RT-PCR for detection and NSP4 gene based genotyping of group A Rotavirus at Virocon 2011.
Y.S.P. Malik, K. Sharma,N.Vaid, R. Singh, A. B Pandey. Occurrence of group A rotavirus mixed G and p genotypes in Bovines: Predominance of G3 genotype in Combination with G8/G10 types at Virocon 2011.
Y.P.S. Malik, S. Chakravarti, N.Vaid, K. Sharma, R.S. Kataria, G.D. Rao, R.K. Singh, AB Pandey. Genetic Characterization and Evolutionary Lineages Analysis of TLR2 and TLR4 of BosGrunniens (Yak) from North Eastern Region, India. In: XVII Annual Convention of Indian Society of Veterinary Immunology & Biotechnology held at Department of Veterinary Microbiology & Biotechnology, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner, Rajasthan.
Y.P.S. Malik, S. Chakravarti, N.Vaid, K. Sharma, R.K. Singh, V. Bhanuprakash, A.B. Pandey. Molecular characterization and evolutionary lineage analysis of Toll like Receptor gene 7 of Bosgrunniens (Yak) and Bosindicus of Uttarakhand, India. In: 5th Uttarakhand State Science & Technology Congress held at Doon University, Dehradun, Uttarakhand from 10th to 12th November 2010.
Chakaravarti, S.K. Biswas, K. Sharma, N.Vaid, R.S. Kataria, G.D. Raj, V. Bhanuprakash, R.K. Singh, B. Mondal, Y.P.S. Malik. Characterization of Toll-like Receptor 4 and 7 exon fragments from BosIndicus of Kumaon (Hill) Region, Uttarakhand. In 10th Indian Veterinary Congress, XVIIth Annual Conference of IAAVR and National Symposium on Newer Challenges in Veterinary Research and Education vis-à-vis Safe Animal, Food and Human Health” College of Veterinary Science & Animal Husbandry, Jabalpur,from 11-12 March 2010.
Y.P.S. Malik, S. Chakaravarti, S.K. Biswas, K Sharma, N.Vaid, R.S. Kataria, G.D. Raj, V. Bhanuprakash, B. Mondal, R.K. Singh. Genetic characterization and evolutionary lineage analysis of Toll-like receptor 7 of Bosgrunniens (Yak) from Himalayan region of India. In XIX National Conference of VIROCON on “Recent trends in viral disease problems and management” held at Dept. of Virology, Sri Venkateswara University, Trupati- 517502, Andhra Pradesh, from 18-20 March 2010.
Y.P.S. Malik, N.Vaid, S. Chakravarti, K. Sharma, S.K. Biswas, B. Mondal, V. Balamurgan, R.K. Singh, V. Bhanuprakash. Genetic characterization and evolutionary lineage analysis of Toll Like Receptor gene 7 of Bosindicus from Hill region of Kumaon, Uttarakhand. In: 4th Uttarakhand State Science & Technology Congress held at GB Pant Agricultural University, Pantnagar, Uttarakhand from 10th-12th Nov, 2009.
NCBI Gene Bank Submissions
Toll Like receptors (TLRs-yak, mithun and cattle)-
Rotavirus-
Picobirnavirus-
Workshop/Traning/Conference attended
.
Protocol training by CRO from Cadila pharmaceuticals limited Ahmadabad for IMP handling, ICH and GCP handling, CRF and ICH process for all the three Clinical trails with Mycobacterium w.
NIDA Clinical Trail Network, online Good Clinical Practice training.
Covid 19 Online learning course education founded in Galway, Ireland by Irish social entrepreneur by Mike Ferick on 21 April 2007.
Participated and Presented paper in Climate conclave 2020 “National conference on Scientific &Environmental Innovation and Implementation of Sustainable Development Goals” during 7-8 March, 2020 Jointly organized by Society for Health, Education &Family Welfare (SHEFWEL) and NIDAN Sarvjan Welfare Society in collaboration with M.P.Council of Science &Technology (MPCST), Bhopal.
Participated in 9
th
Annual Introductory course on Primary Immunodefiency. Diseases,organized by AIIMS, Bhopal in collaboration with Indian Society for Primary Immunodeficiency (ISPID) & Foundation for Primary Immunodeficiency (FPID) on 1
st
March 2020 at AIIMS,Bhopal.
Participated and hands on workshop on “Real-time PCR Molecular Diagnostic workshop towards Strengthening of Laboratory surveillance for Pneumococcal meningitis in India” between 12-14 Feb 2019,at the Christian Medical college, Vellore. The training conducted under the auspices of the Centres for Disease Control and Prevention (CDC, Atlanta) and National Institute of Epidemiology (ICMR).
Participated in 4th Biennial conference of PAi and International symposium on Probiotic Therapy: Translating to Health and Clinical Practice from 16-17 Feb 2018 at All India Institute of Medical sciences New Delhi.
Participated in Two week Faculty Development Programme ”Promoting Innovation of Technology in Science &Technology Institutions”(Technical Teacher’s Traning Programme-TTTP) held from 06 Jan 2018- 19 Jan 2019 at Entrepreneurship Development Cell Madhav Institute of Technology & Science Gwalior (M.P.).
Participated in National conference on “Sustainable Innovation in Life sciences & Environment” on 17th & 18th December Organised by (MP science and Technology COST Bhopal) at Vijayraje Institute of science & Management Gwalior (M.P.)
Participated in National workshop on “Influenza Risk factor Massive Impact and Uncertain future” on Oct 19,2015 at Indian veterinary Research Institute Bareilly under the ageis of Indian Virological society.
Attended International Bio- safety Awareness Programme Including Workshop On Culture of Responsibility on January 11, 2014 at High Security Animal Disease Laboratory, BSL3, IVRI, Bhopal (M.P.).
Trains Trainers in Training cum workshop on 'Conventional and Molecular techniques for diagnosis of trans-boundary animal diseases', Phase 1: 18-25 February, 2014; Phase 2: 28 Feb - 7 March, 2014 at HSADL, BSL3, IVRI, Bhopal.
Attended ICAR-Sponsored short-term training under Niche Area of Excellence on” RECENT ADVANCES IN DISEASE DIAGNOSIS OF LIVESTOCK” Organized at IVRI, Campus Mukteswar from Feb 22-March 2, 2012.
Attended 3 days Hands on Training in” TECHNIQUES IN PROTEOMICS” Jointly Organized by College of Life sciences Gwalior and Helini Biomolecules,Chennai during May 5-7,2011.
Attended National Symposium on” FRONTIERS IN BIOCHEMISTRY AND BIOTECHNOLOGY” held on March 16-17,2012 organised by school of studies in Biochemistry and Biotechnology, Jiwaji university Gwalior(Madhya Pradesh).
Attended workshop on” Molecular & Immunological methods”(ELISA FLOW Cytometry, PCR,RFLP,RAPD and Microarrays) Sponsored by Society for Immunology and Immunopathology at Central Jalma Institute for leprosy and Mycobacterial Diseases.
Membership of professional bodies
Indian Science Congress
Personnel Details
Name- Nirupma Ved
Father’s Name –Ashok Vikram Ved (Advocate)
DOB- 4 Sept 1980
Place- Gwalior (M.P.)
Marital Status –Unmarried
Permanent address- Babai house gopal ganj ORAI, Distt-Jalaun (U.P.) Referees
Dr Y.P.S. Malik
Principal Scientist & ICAR (National
Fellow) Division of Biological
standardization Indian Veterinary Research
Institute Bareilly (U.P.), India
: adjz2i@r.postjobfree.com
Dr G.B.K.S. Prasad
Coordinator & HOD (SOS in Biotechnology)
Jiwaji University, Gwalior (M.P.), India
adjz2i@r.postjobfree.com
Dr. S. Nagarajan
Senior Scientist, OIE Referral Lab for Avian influenza ICAR-National Institute of High security Animal Disease Laboratory Anand Nagar, Bhopal (M.P.), India
: adjz2i@r.postjobfree.com, adjz2i@r.postjobfree.com,adjz2i@r.postjobfree.com
Contact this candidate