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Curriculum Vitae

Ashish Arun Mokate

Ph. D Microbiology, NET

Contact Details :

Ph.No. 940-***-****, 996*******

E mail: adjm2a@r.postjobfree.com

adjm2a@r.postjobfree.com

LinkedIn profile: https://in.linkedin.com dr-ashish-mokate-276298107 Educational profile

• Ph.D. in Microbiology from SRTM University Nanded in 2015

• M.Sc. (Microbiology) with first class from SRTMU Nanded 2008.

• B.Sc. (Microbiology, Biotechnology & Chemistry) first class with distinction from SRTMU Nanded in 2006 with subjects Microbiology, Biotechnology & Chemistry

• NET (LS) 28 December 2018 Conducted by ICAR ASRB Experience:

• 11 years experience as an Assistant Professor Microbiology (UG) Graduate degree student teaching at Indira Gandhi Sr. College, CIDCO, Nanded.

• Freelance International Academic Research writer and Biology International Tutor on Upwork.com from may 2020

• Run a free education YouTube channel Real Biology by Dr. Ashish

• Five years experience as an 8th CBSE to 12th CBSE Biology online tutor

Teaching Methodology

• Marker Board theory writing and use of diagrams

• Use of self prepared cell and organelle models

• Use of ICT : ppt and video as a part of interactive learning 2

Annexure A

Ph.D. Thesis Title: "Production of Lipase by Pseudomonas species"

(http://www.srtmun.ac.in/Thesis/Thesis.aspx) July 2015 Abstract:

Production of Lipase by Pseudomonas aeruginosa species was studied by using normal laboratory media and Agro-industrial wastes as substrates or nutrient sources. For this Pseudomonas aeruginosa Isolates from three different habitats Lonar Lake, Buldhana dist; Arabian sea, Juhu beach, Mumbai & Godavari river, Nanded were isolated, identified using Bergy's manual biochemical tests, using specific growth media and 16s r-RNA sequencing. Lipase production was studied at pH 9.5 (Lonar Lake isolates), pH 8.5 (Arabian Sea isolate) and pH 7 (Godavari river isolate). Assay of Lipase produced was carried out using p- Nitro phenyl acetate as substrate. Characterization of lipases was done, such as effect of incubation period, pH, temperature, carbon sources, nitrogen sources, different oil and oil industry waste substrates, metal ions, organic solvents etc. Using crude partially purified lipase. Later purification was done using techniques salt and solvent precipitation, dialysis, affinity DEAE Cellulose chromatography, SDS-PAGE and at last HPLC. The molecular wt of Lipase from Pseudomonas species L1 was found to be ~60kd. Key words: Pseudomonas species, lipase, Lonar, Arabian sea, river, SDS- PAGE

Professional Skills:

- Isolation, Identification & characterization of bacterial cultures from normal and hyper saline habitats.

- Screening of bacterial cultures for production and assay of primary and secondary metabolites such as enzymes, along with purification and characterization of by using affinity DEAE Cellulose chromatography, SDS-PAGE &/ HPLC.

- Able to work overtime, late night whenever necessary. 3

Hands on Experience in Operating Following Lab Equipment’s: Autoclave (automatic, semi-automatic, manual), Laminar air flow, Incubator, U. V. Chember, Cyclomixer, Orbital Rotary Shaker (REMI), Oven, Research Centrifuge (REMI), Ultra Centrifuge (REMI), HPLC, SDS- PAGE, Chromatography column preparation and operation, Bangalore Genei Electrophoresis & SDS-PAGE apparatus

Publications:

1. A. A. Mokate

1 and S. M. More (Nov. 2013):

Isolation, Identification and Characterization of Pseudomonas Species from Lonar Lake for Production of Lipase; Int. J. Curr. Microbiol. App. Sci. 2(11): 36-42

2. A. A. Mokate

1, S. M. More, V. A. Shinde, S. B. More (2014):

Production of Lipase by Using Pseudomonas sp. L1 Isolated From Meteorite Crater Lake, Lonar; J. Pur. App. Microbiol., Vol. 8 (3): 2445- 2452

3. S. M. More1, A. V. Girde1, A. A. Mokate

1, M. M. V. Baig2 (2012):

Bacitracin Production By Wild and Mutant Isolates of Bacillus licheniformis From Hot Water Springs Of Unkeshwar, Nanded, India; Nat. J. Lif. Sci. Vol. 9(1): 72-76

4. S. M. More1, A. A. Mokate

1, M. M. V. Baig2 (2015):

Utilization of Oil Industry wastes for Production and Partial Purification of Lipase by Using Pseudomonas sp L1 from Alkaline Lake Lonar, India; worldwide Int. Interdisciplinary res. J. Vol. 1(2): 10-20 Personal Particulars:

Corresponding Address: Shri Sudarshan, 742, Ankur Nagar, Bhavsar Chowk, Taroda (Kd.),

Nanded,

Maharashtra State, India.

Pin Code:-431605

Date of Birth: 15 June 1985

Nationality: Indian, Have Indian Passport Marital Status: Married Light Motor Vehicle (LMV) Driving License Valid till July 2022 4

References:

1. Name: Dr. P. K. Dhakephalkar

Designation and Affiliation:

Scientist E, ARI Pune

Phone Number; 952-***-****

Email: adjm2a@r.postjobfree.com

2. Name: Dr. MMV Baig

Designation and Affiliation:

Asst. prof. & H.O.D. Dept. of Biotechnology, Yeshwant Mahavidyalaya, Nanded

Phone Number: 942-***-****

Email: adjm2a@r.postjobfree.com

3. Name: Dr. R. Onkarappa

Designation and Affiliation

Associ. prof. & H.O.D. Dept. of Microbiology,

Sahyadri Science Colleg, Shimoga, Kuvempu

University, Karnataka, India

Phone Number: 944-***-****

Email: adjm2a@r.postjobfree.com

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