Elbashir Araud, MS, PhD

**** *. ******* **., ***. 202, Urbana, IL 61801

Phone (cell): 216-***-**** E mail: adjcha@r.postjobfree.com LinkedIn: https://www.linkedin.com/in/elbashir-araud-58119616b Summary:

Passionate and experienced food scientist seeks opportunity to develop and improve quality products for a health-conscious company. Over eight years of experience in food safety research evaluating traditional and novel inactivation strategies to inactivate foodborne viruses. Determined the inactivation mechanism and parameters of heat, high pressure processing, free chlorine, and UV-irradiation of foodborne viruses in fresh produce and seafood. Experienced in Biosafety Level 2 and 2+

laboratory management. Proficient in molecular biology assays to detect pathogens in food and water samples. Familiar with various databases and software programs, including U.S. Department of Agriculture (USDA) National Nutrient Database, PathogenTracker, and Microsoft Office Suite.

Skills:

• Experienced in heat, high pressure processing, free chlorine, UV-irradiation, and cold plasma inactivation of foodborne viruses in seafood and fresh produce.

• Experienced in isolation and identification of multiple viruses (norovirus, hepatitis A virus, rotaviruses, adenoviruses, and ortherovirus).

• Routinely conducted various molecular biology assays such as RT-PCR, PCR/qPCR, ELISA, plaque assay, functional assay (virus binding assay), SDS-PAGE, primers design, RNA/DNA sequencing, cell and beads protein binding.

• Experienced in working with HPLC.

• Experienced in working on a variety of mammalian and human cell lines (mice macrophage cells RAW-264, MA104, MK-LLC2, human cancer cells A549, vero cells, and insect cells).

• Experienced in bacteria numeration and identification methods in water and food samples, including total count plates, membrane filtration, MPN, and bacterial biochemical properties by API system and selective media. Education:

• Microbiology and Virology, PhD August 2015

• The Ohio State University (Columbus, OH)

• Department of Food Science & Department of Veterinary Biosciences

• Agricultural Microbiology, MS July 2004

• Cairo University (Cairo, Egypt)

• Food Science and Technology, BS July 1991

• Higher Institute of Technology (Brack, Libya)

Work Experience:

• BioNanotechnology Laboratory Interim Manager Oct. 2019– Present University of Illinois at Urbana-Champaign

Holonyak Micro and Nanotechnology Laboratory

• Managed laboratory safety and biological hazard waste procedures.

• Prepare the laboratory for DRS auditing.

• Train graduate students to utilize shared laboratory properly.

• Managed common use calendar to avoid time conflicts.

• Post-Doctoral Research Associate Nov. 2015 – Apr., 2019 University of Illinois at Urbana-Champaign

Department of Civil and Environmental Engineering

• Investigated the stability of human enteric viruses (rotavirus, human norovirus, orthoreovirus, and adenovirus) in wastewater and fresh produce.

• Studied the mechanism of human enteric viruses’ inactivation under solar irradiation (UV-A and visible light), low and medium pressure UV light, cold plasma, and monochromatic UV in food and environmental samples.

• Developed PMG-MG method to mimic the viruses’ attachment to the host cell receptor.

• Compared viral sequences from clinical feces samples with viral sequences available on GenBank

• Trained and supervised 7 undergraduate students, 3 MS students, and 4 Ph.D. students, sharing expertise and knowledge on virus-related experiments, data collection, and analysis.

• Graduate Research Assistant, PhD

The Ohio State University

Department of Food Science and Technology

Department of Veterinary Biosciences

• Investigated the stability and distribution of enteric viruses (Hepatitis A virus, human norovirus, rotavirus, Tulane virus, and murine norovirus) in seafood

(oysters).

• Determined mechanism of viral inactivation by thermal and non-thermal processing High Hydrostatic Pressure technologies.

• Investigated the mutation in enteric viruses genome that help in develop heat and high pressure resistance using Tulane virus as a model.

• Graduate Research Assistant, MS

Cairo University

Department of Agricultural Microbiology

• Produced single cell protein, alcohol, and cellulase enzymes from agricultural wastes by yeasts (Saccharomyces cerevisiae), fungi (Trichoderma viride, Trichoderma reesei, Phanerochaete chrysosporium), and bacteria (Bacillus cellobiose).

• Produced Saccharomyces cerevisiae from molasses and barley waste hydrolyses. Professional Development & Leadership Experience:

• BioNanotechnology Laboratory Interim Manager at Nick Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign

• Oct., 2019– Present

• Chair of Botany program at University of El Gabal El Gahrby, Libya.

• August 2006 – August 2008

Projects:

• Identification of effective and practical methods for microbial disinfection in wastewater for water reuse (Funded by EPA).

• Ensure the microbial safety of seafood (Funded by USDA).

• Reduction of norovirus burden in food (Funded by NoroCORE).

• Safe recycling of agricultural wastes (Funded by Cairo University). Teaching Experience:

• Graduate Teaching Assistant

The Ohio State University

Dept. of Food Science and Technology

o FDSCTE 2400: Introduction to Food Processing.

o FST 836: Advanced Food Microbiology II (Food Virology and Immunology). Publications:

Barakat, Olfat, S.; El Gizawy, S. A.; Araud, E.; Zahra, M. K., Biological Conversion of Barley Straw into Glucose for Baker’s Yeast Biomass Production. Mansoura Journal of Agricultural Science 2005, 30, (12), 8053 8073.

Araud, E.; DiCaprio, E.; Yang, Z.; Li, X.; Lou, F.; Hughes, J. H.; Chen, H.; Li, J., High Pressure Inactivation of Rotaviruses: Role of Treatment Temperature and Strain Diversity in Virus Inactivation. Applied and Environmental Microbiology 2015, 81, (19), 6669-6678. Araud, E.; DiCaprio, E.; Ma, Y.; Lou, F.; Gao, Y.; Kingsley, D.; Hughes, J. H.; Li, J., Thermal Inactivation of Enteric Viruses and Bioaccumulation of Enteric Foodborne Viruses in Live Oysters (Crassostrea virginica). Applied and Environmental Microbiology 2016, 82,

(7), 2086-2099.

Araud, E.; Shisler, J.L.; Nguyen, T.H., Inactivation mechanisms of human and animal rotaviruses by solar irradiation. Environ. Sci. Technol. 2018, 52, (10), 5682-5690. Leone, C.; Dharmasena, M.; Tang, C.; DiCaprio, E.; Ma, Y.; Araud, E.; Bolinger, H.; Rupprom, K.; Yeargin, T.; Li, J.; Schaffner, D.; Jiang, X.; Sharp, J.; Vinje, J.; Fraser, A., Prevalence of Human Noroviruses in Commercial Food Establishment Bathrooms. Journal of Food Protection 2018, (81), 719–728.

Sun, P. P.; Araud, E.; Huang, C.; Shen, Y.; Monroy, G. L.; Zhong, S.; Tong, Z.; Boppart, S. A., Eden, J. G.; Nguyen, T. H., Disintegration of Simulated Drinking Water Biofilms with Arrays of Microchannel Plasma Jets, npj Biofilms and Microbiomes 2018, (18), 4-24. doi: 10.1038/s41522-018-0063-4. eCollection 2018.

Wang, Y., Araud, E., Thanh, T.H., Yuan, B., Inactivation of MS2 bacteriophage by UV disinfection in water containing algal organic matter. Chemosphere. 2018 Sep 17;214:195-202. doi: 10.1016/j.chemosphere.2018.09.065. [Epub ahead of print] Fuzawa, Miyu; Araud, Elbashir; Li, Jianrong; Shisler, Joanna ; Nguyen, Thanh, Free Chlorine Disinfection Mechanisms of Rotaviruses and Human Norovirus Surrogate Tulane Virus Attached to Fresh Produce Surfaces. ES&T manuscript es-2019-03461d (Accepted). Araud, E., Fuzawa, M., Li, J., Shisler, J.L.; Nguyen, T.H., UV inactivation of rotavirus and Tulane virus targets different components of the virions. Applied and Environmental Microbiology, 2019 (AEM01384-19) Accepted.

Conferences/Presentations:

Araud, E.; Li, J., Distribution and Inactivation of a Human Norovirus Surrogate and Hepatitis A Virus in Oysters, International Association of Food Protection Annual Meeting, Milwaukee, WI July 31-August 3, 2011. Poster.

Araud, E.; Li, J., High pressure Processing of Rotaviruses: The Roles of Strain Diversity and Treatment Temperature in Virus Inactivation. International Association of Food Protection Annual Meeting, Providence, RI, Jul 22- 25, 2012. Oral presentation. Araud, E.; Guest, J.S.; Shisler, J.; Nguyen, T.H., The Impact of Strain Diversity on Rotavirus Solar Irradiation Disinfection. 2016 23rd Annual Midwest Microbial Pathogenesis Conference, Urbana-Champaign, IL, September 24-25, 2016. Poster. Araud, E.; Li, J., Identification of Effective and Practical Thermal and Non-thermal Processing Technologies to Inactivate Major Enteric Viruses. Environmental Engineering and Science, Program Seminar, University of Illinois at Urbana-Champaign, IL, January 25, 2017. Invited talk.

Araud, E.; Guest, J.S.; Shisler, J.; Nguyen, T.H., The Mechanism Underlying the Solar Irradiation Disinfection of Rotaviruses and MS2. 2017 Water Microbiology Conference and the 19th IWA-HRWM Symposium, Chapel Hill, NC, May 15-18, 2017. Oral presentation. Araud, E.; Guest, J.S.; Shisler, J.; Nguyen, T.H.; The Mechanism Underlying the Solar Irradiation Disinfection of Rotaviruses and MS2. The Association of Environmental Engineering and Science Professors (AEESP) Research and Education Conference, Ann Arbor, MI, June 20-22, 2017. Poster.

Lardizabal, A.T.; Araud, E.; Nguyen, T.H.; Guest, J.S., Wavelength- dependent mechanistic modeling of indirect, exogenous virus inactivation to predict UV and solar- based disinfection efficiency. The Association of Environmental Engineering and Science Professors (AEESP) Research and Education Conference, Ann Arbor, MI, June 20-22, 2017. Poster. Araud, E.; Nguyen, T.H., Inactivation mechanisms of human and animal rotavirus by solar irradiation. Environmental Engineering and Science, Program Seminar, University of Illinois at Urbana-Champaign, IL, January 25, 2018. Invited talk. Affiliations:

• Food Science Club at The Ohio State University 2010 – present

• Member

• Institute of Food Technologists 2011 – present

• Member

• Gamma Sigma Delta – The Honor Society of Agriculture 2013 – present

• Member

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