ASMITA NANDI

Email: adirve@r.postjobfree.com, Contact: 813-***-****, LinkedIn: https://www.linkedin.com/in/asmita-nandi-b682b416a/ OBJECTIVE

Self-motivated and hard-working Biotechnology Engineering professional actively seeking exciting opportunities in research and development of therapies and consequent drug discovery and formulation for treating diseases, wherein my skills will contribute to the fulfillment of an organization’s goals and aspirations by gaining relevant knowledge and experience EDUCATION

Masters of Science (Biotechnology-3.5) University of South Florida (USF); Tampa, Florida [2019-2021] Courses: Aging and Neuroscience, Methods in Molecular Biology, Translational biotechnology, Applied bioinformatics Bachelor of Technology (Biotechnology-3.7) Chaitanya Bharathi Institute of Technology (CBIT); India [2015-2019] Courses: Cell biology, Molecular Biology, Genetics, Bioinformatics, Medical biotechnology, Cancer biology, Genetic Engineering

SKILL SET AND TOOLS

Programming language: C, C++ basics

Computer skills: Microsoft Word, Microsoft PowerPoint, Microsoft Excel Techniques: SDS-PAGE, Agarose electrophoresis, PCR, FRAP assay, Comet Assay, Thioflavin-T assay, DDPH assay and ABTS assay, Pareto analysis, Single radial & ouchterlony double immunodiffusion, ELISA, DNA & RNA isolation, serial dilution, quadrant streaking and spreading, C-DNA synthesis, western blotting; Thin layer, paper and Column chromatography Bioinformatics tools: BLAST and ClustalΩ, Chimera, Marvin sketch, MODELLER, Swiss PDB viewer, PROCHECK, Primer3, Autodock and Igemdock

EXPERIENCE

Molecular Biology Intern, Ceincia Life Sciences (Hyderabad, India) [May 2019-July 2019]

• Performed isolation of DNA, prepared stock solutions, standardized assays and working protocols

• Executed SDS-PAGE, Agarose electrophoresis and PCR

• Conducted immunodiffusion experiments

Project Assistant, Nizam’s Institute of Medical Sciences (NIMS) (Hyderabad, India) [Dec 2018- April 2019] Title: “Evaluation of Risk Factors for Temporal Lobe Epilepsy and Assessment of Surgical Therapy”

• Narrowed down five risk factors linked to epilepsy, formulated case sheets for individuals with refractory epilepsy in Hyderabad, India

• Statistical analysis was done to postulate most common risk factor and to assess surgical therapy via a patient inventory-

[Quality of life in Epilepsy-(QOLIE-31)]

Bioinformatics Intern, Ceincia Life Sciences (Hyderabad, India) [Aug 2018-Nov 2018]

• Familiarized with various biological databases, visualization by chimera, sketched structures with Marvin sketch, performed sequence alignment with BLAST and ClustalΩ

• Performed docking with Autodock and Igemdock

• Accomplished homology modeling with MODELLER, Swiss PDB viewer, PROCHECK and Primer3 Summer Research Fellow, University of Calcutta (Kolkata, India) [May 2018-July 2018] Title: “Identifying flavonoids with anti-amyloidogenic properties and evaluating their antioxidant potential” (Science Academies’ Summer Research Fellowship Programme (IASc-INSA-NASI))

• Estimated anti-amyloidogenic potential of flavonoids using Thioflavin-T assay

• Performed DDPH assay and ABTS assay after standardization to estimate antioxidant potential of flavonoids

• Conducted molecular mechanic simulations for the flavonoids in study, visualized structures in Pymol Microbiology Intern, Ceincia Life Sciences (Hyderabad,India) [Dec 2017-April 2018]

• Performed isolation of bacteria from various sources, serial dilution, preparation of growth media, purification by streaking and spreading techniques

• Executed primary screening tests such as catalase test, gram staining, endospore staining, oxidase test

• Conducted secondary screening tests such as carbohydrate fermentation & gas production, citrate utilization, H2S production, colony morphology and characteristics

Summer Intern, Rajiv Gandhi Centre for Biotechnology (RGCB) (Trivandrum, India) [June 2017-July 2017]

• Acquired hands on experience on various protocols conducted in the lab

• Familiarized with the working of PCR

• Gained insight into the various processes such as DNA isolation, C-DNA Synthesis, electrophoresis, western blotting, SDS-PAGE, maintenance of cell lines etc

EXTRA-CURRICULAR ACTIVITIES

• Active vocal training in Hindustani classical music from Smt. Sudeshna Gupta “KOMAL GANDHAR”; India

• Member of the organizing team of the technical symposium of CBIT “Sudhee-Neozion” 2018; India

• Member of the organizing committee for the literary event of CBIT, “LITERATI” 2016; India

• Deputy Head of councils, ensured smooth functioning of CBIT MUN (Model United Nations 2016); India

• Completed all the levels in Abacus, a tool used to solve math part of skill development programme under SIP Academy; India

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