Van Le

Location: Worcester, MA Email: adilil@r.postjobfree.com

Phone: 224-***-****

SUMMARY (Youtube link: https://youtu.be/hEh-F4g8fdw) Third-year Undergraduate Biology & Biotechnology Student with 3+ years of hands-on experience conducting laboratory experiments and developing innovative solutions to optimize research and make new discoveries within the biotechnology field. Motivated self-starter with a passion for delivering data-driven insights through advanced testing and laboratory work. SKILLS

● Research: Bacteria & Mammalian Cell Culture, Staining & Counting, Western Blot, ELISA, BCA, RT-qPCR, PCR, cDNA/RNA isolation, Flow Cytometry, IMAC, SEC, DNA Recovery & Purification, Plasmid & Oligo Design, Plasmid Miniprep, Transduction, Transformation, Biochemical Tests (Gram Positive/ Negative), Gel Electrophoresis, Tissue Dissection, Gibson Assembly, Immunostaining, Uv/Vis Spectrophotometry, Media and Plates preparation

● Leadership: Innovation, Project Management, Prioritization, Process Improvement, Cross-functional Collaboration

● Software & Equipment:LabArchives, BLAST, Benchling, Quartzy,Thermal Cycler, NanoDrop, GloMax Microplate Reader, Seahorse XF96, Countess II Automated Cell Counter, Cytation 5, Azure Biosystems, Licor Odyssey, Autoclave

● Languages: Fluent - English/Vietnamese, Intermediate - Mandarin EDUCATION & AWARDS

● Bachelor of Science in Biology & Biotechnology, Worcester Polytechnic Institute (WPI), GPA 3.8/4.0 May 2022

Relevant Coursework: Cell Biology, Microbiology, Anatomy & Physiology, Genetics, Molecular Biology & Genetic Engineering, Biochemistry, Organic Chemistry

RELEVANT EXPERIENCE

Research Assistant, Shell Lab WPI February 2020 - Present

● Conduct own project: Study the effects of TetR and tetO on mRNA half-life and protein degradation rate in Mycobacterium smegmatis to understand Tuberculosis and the cause of long-term therapy due to its stress tolerance.

● Conduct plasmid & oligo design, Gibson Assembly, plasmid purification, plasmid miniprep, PCR, RT-qPCR, Flow cytometry, DNA recovery and purification, E.coli & M. smegmatis transformation, cell culture, preparation of competent cells, MIC. Research Intern, Lucy Therapeutics May 2020 - August 2020

● Researched GPCR signaling pathways & mitophagy for drug development for neurodegenerative diseases by conducting cell lysis, BCA assay, cell culture & compound treatment, Seahorse XF96, and Complex V assay & presented testing data results to the 5 person research team.

● Studied the mechanisms of PINK1-PARK2-mediated mitophagy and the effect of mitochondrial quality control on pathways in different cell lines when treated with different compounds.

● Collaborated with the VP of Research and Director of Mitochondrial Biology on a Western Blot experiment.

● Utilized larger amounts of proteins, overnight incubation for primary antibodies at 4 C, and appropriate exposure times to provide sharp bands and clearer backgrounds to use the Western Blot experiment on more cell lines and antibodies.

● Assisted Research Associates with passaging and maintaining fibroblasts and Hela cells.

● Wrote, tested, and optimized SOP for Western Blot for fibroblasts, Hela cells, and glial cells detected by Azure Biosystems & Licor Odyssey to establish adenosine receptor profiles necessary for in vitro and in vivo testing later in development.

● Founded a Journal Club that presented a journal article every week to the board of experts to discuss the latest developments and studies in mitophagy and autophagosomes and their impact on current and future experiments. Veterinary Lab Technician, Beechnut Animal Hospital May 2019 - June 2019

● Assisted doctors, veterinary technicians, and 30+ animal patients per day with CBC, urinalysis, ear cytology, fecal flotation, Gram stain & parvovirus, heartworms tests, X-ray, dental radiography, and ultrasound. Lab Assistant - Professor Roberts and Professor Buckholt, WPI 2018 - 2020

● Set up, organized, and maintained a biology teaching and research lab by ensuring a clean and sterile environment, stocking and preparing chemicals, formulating solutions, plate pouring, and waste disposal to streamline operations class to class. SELECT PROJECT

The Race Against Antibiotic Resistance in 7 weeks, Professor Michael Buckholt and Louis Anthony Roberts

● Identified novel antibiotics produced by soil bacteria collected from areas around Worcester, MA, and hypothesized that these microbes had the potential antimicrobial ability to stop ESKAPE relatives from reproducing or destruction.

● Discovered that 1 of 12 isolated strains produced antibiotics active against E. coli and K. pneumoniae by serial dilutions, master plating, isolate testing, 16s gene PCR, gram stain, organic extraction, extract testing, and biochemical assays. 10/23/2020 Unofficial Transcript

https://bannerweb.wpi.edu/pls/prod/bwskotrn.P_ViewTran 1/4 Unofficial Transcript

522596438 Van Le

Oct 23, 2020 01:12 pm

100 Institute Rd

Worcester, MA 01609

This is ** NOT ** an official transcript.

Please Note:

Courses "in progress" appear at the end of this report. For undergraduate activity,

1/3 unit activity is represented as 3.00 credit hours. 1 Unit of activity is represented as 9.00 credit hours. Other unit values are represented proportionately. Information on minors and second majors is not displayed on this page. The undergraduate GPA is not reflected on the Transcript. The 'Grade Point Average for Passed Courses' appears on the Degree Audit web page. Transfer Credit Institution Credit Courses in Progress Transcript Data

STUDENT INFORMATION

Name : Van Le

Birth Date: Apr 08, 1999

Curriculum Information

Current Program

Major: Biology and

Biotechnology

***This is NOT an Official Transcript***

DEGREES AWARDED

Degree

Sought:

Bachelor of

Science

Degree Date:

Curriculum Information

Primary Degree

Major: Biology and Biotechnology

Sufficiency: CDR grade=A : Chinese language

TRANSFER CREDIT ACCEPTED BY INSTITUTION -Top-

xxx: Advanced Placement

Subject Course Title Grade Credit

Hours

R

BB 1000 BIOLOGY ELECTIVE L 3.000

10/23/2020 Unofficial Transcript

https://bannerweb.wpi.edu/pls/prod/bwskotrn.P_ViewTran 2/4 CH 1010 MOLECULARITY L 3.000

MA 1021 CALCULUS I L 3.000

MA 1022 CALCULUS II L 3.000

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 0.000 0.000 12.000

Unofficial Transcript

INSTITUTION CREDIT -Top-

Term: Fall 2018

Major: Biology and Biotechnology

Additional Standing: Dean's List

Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

BB 1025 01 HUMAN BIOLOGY B 3.000

CH 1020 01 CHEMICAL REACTIONS A 3.000

CN 1541 01 ELEMENTARY CHINESE I A 3.000

CN 1542 01 ELEMENTARY CHINESE II A 3.000

ISE 1801 01 COMPOSITN FOR NON-SPK

ENGLISH

A 3.000

MA 2611 01 APPLIED STATISTICS I A 3.000

PE 1207 01 CLUB SPORT - KARATE A 0.750

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 18.750 18.750 18.750

Cumulative: 18.750 18.750 18.750

Unofficial Transcript

Term: Spring 2019

Major: Biology and Biotechnology

Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

BB 2003 01 FUNDAMENTALS OF

MICROBIOLOGY

A 3.000

BB 2550 01 CELL BIOLOGY A 3.000

BB 2903 01 ANATOMY AND PHYSIOLOGY A 1.500

CH 1030 01 KINETICS, EQUILIB & THERMO C 3.000

CN 1543 01 ELEMENTRY CHINESE III A 3.000

CN 2541 01 INTERMEDIATE CHINESE I A 3.000

ISE 2800 01 COLLEGE WRTNG FOR NON-SPK

ENGL

A 3.000

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 19.500 19.500 19.500

Cumulative: 38.250 38.250 38.250

Unofficial Transcript

Term: Fall 2019

Major: Biology and Biotechnology

10/23/2020 Unofficial Transcript

https://bannerweb.wpi.edu/pls/prod/bwskotrn.P_ViewTran 3/4 Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

BB 2915 01 SEARCH FOR SOLUTIONS IN

SOIL

A 3.000

CH 2310 01 ORGANIC CHEMISTRY I B 3.000

CN 2542 01 INTERMEDIATE CHINESE II A 3.000

CN 2543 01 INTERMEDIATE CHINESE III A 3.000

PE 1099 01 HEALTHY ALTERN: ZUMBA A 0.750

PH 1110 01 GENERAL PHYSICS-MECHANICS C 3.000

PSY 1400 01 INTRO TO PSYCHOLOGICAL

SCIENCE

A 3.000

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 18.750 18.750 18.750

Cumulative: 57.000 57.000 57.000

Unofficial Transcript

Term: Spring 2020

Major: Biology and Biotechnology

Additional Standing: Dean's List

Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

BB 1035 01 BIOTECHNOLOGY A 3.000

BB 2904 01 ECOLOGY, ENVIRONM, & ANIM

BEHV

A 1.500

BB 2920 01 GENETICS A 3.000

BB 3511 01 NERVE AND MUSCLE

PHYSIOLOGY

A 1.500

BB 3527 01 MOLECULAR BIO & GENETIC ENG A 3.000

CH 2320 01 ORGANIC CHEMISTRY II A 3.000

CH 2330 01 ORGANIC CHEMISTRY III A 3.000

PE 1055 01 PHYSICAL CONDITIONING A 0.750

PE 1207 01 CLUB SPORT - KARATE A 0.750

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 19.500 19.500 19.500

Cumulative: 76.500 76.500 76.500

Unofficial Transcript

Term: Summer 2020

Major: Biology and Biotechnology

Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

INT 1000 01 INTERNSHIP AT 0.000

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 0.000 0.000 0.000

Cumulative: 76.500 76.500 76.500

10/23/2020 Unofficial Transcript

https://bannerweb.wpi.edu/pls/prod/bwskotrn.P_ViewTran 4/4 RELEASE: 8.7.1

Unofficial Transcript

Term: Fall 2020

Major: Biology and Biotechnology

Subject Course Level Title Grade Credit

Hours

Start and

End Dates

R CEU

Contact

Hours

BB 2050 01 ANIMAL BEHAVIOR A 3.000

BB 2902 01 ENZYMES, PROTEINS, &

PURIFICTN

A 1.500

BB 3003 01 MEDICAL MICROBIOLOGY A 3.000

BB 3101 01 HU ANATMY & PHYS: MVMT &

COMUN

A 3.000

CH 4110 01 PROTEIN STRUCTURE &

FUNCTION

A 3.000

Term Totals (Undergraduate)

Attempt

Hours

Passed

Hours

Earned

Hours

Current Term: 13.500 13.500 13.500

Cumulative: 90.000 90.000 90.000

Unofficial Transcript

COURSES IN PROGRESS -Top-

Term: Fall 2020

Major: Biology and Biotechnology

Subject Course Level Title Credit Hours Start and

End Dates

BB 3080 01 NEUROBIOLOGY 3.000

ID 2050 01 SOC SCI RES-IQP-WORC MA (WI) 3.000

Unofficial Transcript

Term: Spring 2021

Major: Biology and Biotechnology

Subject Course Level Title Credit Hours Start and

End Dates

BB 2030 01 PLANT DIVERSITY 3.000

BB 3010 01 SIMULATION IN BIOLOGY 3.000

CH 4140 01 METABOLISM AND DISEASE 3.000

PC 1000 01 PROJECT CENTER - WORCESTER 0.000

Unofficial Transcript

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