ANIL KUMAR RAM

Correspondence Address:

Lab- **

Department of Immunopathology,

Post Graduate Institute of Medical Education and Research, Chandigarh, India

no: +91-921*******

Email Id: adijly@r.postjobfree.com

Present Status

I am a PhD student and going to submit my thesis on or before 30th September, 2020 under the supervision of Dr. Yashwant Kumar, at department of Immunopathology, PGIMER,Chandigarh. My thesis is on etiopathogenesis of biliary atresia a most common cholestatic disorder in children with unknown etiology. The recent hypothesis is that it could be a virus induced an autoimmune disorder. I have tried to find out the viral (CMV, EBV, Rotavirus and Reovirus) association in terms of viral load by quantifying the viruses using real time PCR and ELISA. In addition, we have also found differential protein profile in patients compared to control by using two proteomic approaches like 2D Gel electrophoresis based MALDI TOF/TOF and LC MS/MS based mass spectrometry. Educational Qualification

• M.Sc. (Microbiology): Jiwaji, Gwalior (M.P) in 2010.

• B. Sc: of Calcutta in 2004.

National level examinations qualified

• Indian Council of Medical Research (ICMR) Examination for Junior Research Fellow 2013.

• Council of Scientific and Industrial Research (CSIR) – National Eligibility Test for Lecturer ship (Dec 2010).

• Graduate Aptitude Test in Engineering (GATE) - Life Sciences (2010 & 2011). M.Sc. Thesis

“Molecular and In-silico analysis of Antigen 84 gene Rv 2145c of Mycobacterium tuberculosis” under the supervision of Dr. Kiran Katoch, National JALMA Institute for Leprosy and Other Mycobacterial Diseases

(ICMR), Agra, India.

Work Description: I did molecular characterization of Rv 2145c gene in Multiple drug resistant tuberculosis clinical isolates by PCR amplifications and restriction digestion (RFLP). In-silico analysis of the Antigen 84 gene was carried out by NEB Cutter (Ver2.0) (tools.neb.com/NEB cutter2/index.php) and ClustalW Multiple Sequence Alignment Tool (www.ebi.ac.uk/services/clustalw) and the docking analysis of Ag84 protein with primary and secondary line drugs against Mycobacterium tuberculosis by using Firedock server

(bioinfo3d.cs.tau.ac.il/FireDock/) and www.ks.uiuc.edu/Research/vmd. Research Experience

Having a total of two years nine months research experience in Life Sciences (after M.Sc).

• From 1st January 2012 to 30th November 2013 worked as Junior Research Fellow in Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India on Indo-US collaborated project entitled “Rapid diagnosis of early TB in HIV patients” Work description: TB is one of the primary causes of morbidity and mortality in HIV+ patients mainly due to lack of ability to diagnose the disease in early stage. This project was a step towards the development of a rapid, simple Point of Care (POC) test that can accurately identify early TB and can be used to routinely monitor HIV+ patients with TB. As a Junior Research Fellow, I have screened serum samples of -naive HIV+ patients for tuberculosis with purified recombinant Mycobacterial antigenic proteins by Indirect ELISA. During this tenure I did Mycobacetrial DNA extraction, recombinant vector formation using bacterial plasmid and mycobacterial genes, Colony PCR, recombinant vector transformation, expression, purification and dialysis of Mycobacterial genes

(ESAT6, CFP10, MS, and MPT51).

• From 15th February 2011 to 31st December 2011 worked as Research Assistant in Department of Biotechnology, Indian Institute of Technology (IIT), Roorkee on project entitled “Ganga Basin Management Plan” funded by Ministry of Environment and .

ANIL KUMAR RAM

Work description: In Indian sub-continent, the Ganga ecosystem supports 25,000 or more species ranging from microorganisms to mammals and serves as lifeline for the population of over 500 million people. As a Research Assistant of this project I have collected and analysed the secondary data regarding animal diversity (zooplankton, nematodes, oligochaetes,, molluscs, amphibians, reptiles, fishes and etc.) from different books, thesis, papers and reports.

Experimental Skills

• Molecular Techniques: Isolation of DNA and RNA from various pathological samples (Blood, urine, biliary tissue and liver biopsies), Agarose gel electrophoretic analysis of DNA and RNA. Primer designing, Conventional PCR, qRT PCR (quantification of Cytomegalovirus, Epstein-bar virus, Rotavirus and Reovirus)

• Gene Cloning and Protein purification: Recombinant vector formation, transformation, gene expression, protein purification, protein estimation and protein dialysis, ELISA etc.

• Chromatographic Techniques: Paper Chromatography, Thin layer Chromatography and Column chromatography (Ni 2+ NTA).

• Protein Techniques: One Dimension SDS-PAGE, 2D Gel Electrophoresis, MALDI TOF/TOF Mass Spectrometry, LC MS/MS Mass Spectrometry, Western blotting etc.

• Cell Culture/ Microscopy/Immunology: Primary and continuous cell culture, Estimation/Cell death and cell viability assays (MTT and DAPI staining) and FACS.

• Microbiological Techniques: Isolation and characterization of microorganisms, microbial cultures and storage, handling of Bio Safety Laboratory Level II & III, Mycobacterium laboratory practices (Culture and Drug sensitivity test).

• Additional Skills: Efficient in Microsoft, PowerPoint, Excel, GraphPad Prism for statistical analysis, Mendeley and Endnote for Referencing.

Training Attended

• Hands-on Training Workshop on “Basic Cell Culture Technology” from 16th May to 19th May 2016 at National Centre for Cell Sciences (NCCS) Pune.

• Microbiological and Molecular Techniques at National JALMA Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Agra (UP).

• MaxQuant Summer - July 2017 on “Quantitave Proteomics applied to Life Sciences” held at Harnack of the Max Planck Society, Berlin, Germany.

• “Advanced Techniques in Protein Design and Engineering” March 2016 held at IISER, Mohali, Punjab.

• National workshop on “Analytical phytopharmaceutical by High Performance Liquid Chromatography-Mass Spectrometry” from 5th December to 9th December 2016 at SAIF, CSIR-CDRI., Lucknow.

Oral/Poster Presentation

ANIL KUMAR RAM

• Oral Presentation: Topic entitled “Proteomic analysis of blood and atretic bile duct reveals candidate markers for biliary atresia” held at 27th Annual Conference of Asian Pacific Association for the Study of the Liver

(APASL) Annual Meeting 2018, New Delhi.

• Poster Presentation: Topic entitled “High prevalence of cholangiotropic virus in biliary atresia” held at Keystone, Colorado, USA.

• Poster Presentation: Topic entitled “Differential Plasma Proteome Profile of Biliary Atresia in Infants: A Study” held at MaxQuant Summer - July 2017, Harnack of the Max Planck Society, Berlin, Germany.

• Poster Presentation: Topic entitled “High prevalence of Cryptococcus neoformans in droppings: a threat to Chandigarh population” presented in Research Scholar Convention-2K15, Punjab, Chandigarh. Paper Publication

1. An improved and easy protocol for primary epithelial cell culture from atretic tissue in biliary atresia. Sanghamitra Machhua, Yashwant Kumar, Ravi Prakash Kanojia, Alka Bhatia, Babu Ram Thapa, Ranjana Walker Minz, Anil Kumar Ram, Veena Dhawan. Tissue and Cell, Volume-56, 2019, Pages 83-89, ISSN 0040-8166, https://doi.org/10.1016/j.tice.2019.01.001. 2. Effect of Dietary Advanced Glycation end Products on Cell Cycle of Peripheral Blood Lymphocytes. Anupriya Chhabra, Anil Kumar Ram, Alka Bhatia1 and Sumit Goel. Journal of Blood and Lymph (2018), Volume 8, Issue 2, Pages 1-3, DOI:10.4172/2165-7831.1000211. 3. Prevalence of Cryptococcus neoformans in dry droppings of domestic pigeons in Chandigarh. Anil Kumar Ram., Yashwant Kumar., Biman Saikia., Ranjana Walker Minz. International Journal of Current Advanced Research (2018) Volume 7; Issue 2(J); February 2018; Page No. 103**-*****. DOI: http://dx.doi.org/10.24327/ijcar.2018

4. Increased advanced glycation end product specific fluorescence in repeatedly heated used cooking oil. Anupriya Chhabra, Alka Bhatia, Anil Kumar Ram, Sumit Goel. Journal of Food and Technology

(2017). 54(8):2602–2606 DOI 10.1007/s13197-017-2682-9. 5. Micronutrients as predictors of tuberculosis development in HIV infected patients” in Journal of acquired immune deficiency syndrome” (In communication)

Personal Profile

Father’s Name : Late Ram Ashis Ram

Date of Birth : 12 Jan 1985

Gender : Male

Marital status : Married

Permanent Address : Vill-Bagahwa, Po-Semariya, Dist- Gopalganj (Bihar) Declaration: It is certified that all information given by me above is true to the best of my knowledge. If any information were found false, I am aware that my application is liable for cancellation. Date: 23-8-2020

Place: Chandigarh (Anil Kumar Ram)

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