Andrew F. Grube

adifxm@r.postjobfree.com

https://www.linkedin.com/in/andrew-grube

508-***-****

** **** **. #* **********, MA 02143

Professional Summary

Bioprocess engineer with 8 years of experience in the biotech industry. Focused in upstream cell culture process development and manufacturing. Proficient in small scale and manufacturing scale bioprocesses.

Education

Northeastern University - Boston, Massachusetts

Bachelor of Science in Chemical Engineering, Minor in Biochemical Engineering, May 2013

Honors Distinction, Cum Laude

Professional Experience

Codiak BioSciences – Cambridge, MA

Principal Associate Scientist July 2018 – Present

Sr. Associate Scientist June 2016 – July 2018

Continuous Processing Experience

Developed perfusion platform process for continuous production of native exosomes. Successfully applied perfusion platform to engineered exosome constructs

Technical lead for process transfer of perfusion process to CMO. Collaborated on writing and reviewing of BOM, batch records and tech transfer documents

Collaborated with vendors for custom solutions to engineering challenges

Supported manufacturing as onsite SME during two successful GMP campaigns

Successfully scaled up bench scale perfusion process to GMP 500L scale for production of clinical material for two engineered exosome constructs

Other Experience

Developed platform fed-batch process for batch production of exosomes

Reviewed batch records, wrote technical reports, and provided remote and onsite technical support for CMO for fed-batch engineering and GMP runs

Cross-trained in UF/DF and chromatography downstream unit ops

Prepared media, buffers, and feeds for general usage and evaluation of new formulations

Coordinated purchase, installation, and testing of benchtop bioreactors and Thermo 50L SUB (Finesse DeltaV infrastructure)

Coordinated installation of Seeq software to enhance real-time data collection and analysis capabilities

Participated in writing and review of upstream CTA sections

Biogen – Cambridge, MA

Engineer I July 2015 – June 2016

Associate Engineer I July 2013 – July 2015

Performed batch and fed-batch cell culture in shake flasks, 24 deep well, AMBR, WAVE, and 5L bioreactors

Performed clone screening and DOE experiments using ambr15 microbioreactors

Worked with ATF and TFF systems to optimize perfusion cultures at 5L bench scale

Supported manufacturing investigations with small scale bioreactor experiments

Tested and wrote protocols for pCO2 probes and ATF2

Developed improved media and feed formulations

Recorded and managed data in electronic lab notebook. Developed enhancements for greater functionality

Worked with pilot plant, manufacturing, and tech transfer teams to generate PFDs and transition programs to manufacturing

Acceleron Pharma – Cambridge, MA

MLSC Summer Intern, May – July 2013

Assisted with GMP fed-batch processes at 500L scale

Helped review and revise documents and batch records related to manufacturing campaign

Performed scale-down protein purification development

Acceleron Pharma – Cambridge, MA

Bioprocess Engineering Co-op, January – July 2012

Operated and monitored 250L Thermo SUB in a GMP manufacturing facility following SOPs and batch records

Performed fed-batch processes from thaw to harvest of 250L SUB bioreactor

Conducted scale-down experiments in 50L SUB, 3L glass spinners, WAVE, and shake flasks

Ran routine cell counting, nutrient, metabolite, and HPLC Protein-A assays

Performed cell banking of research CHO cell lines

Maintained and automated data analysis using Visual Basic and Excel

Helped review and revise SOP documents

Genzyme – Framingham, MA

Bioengineering Co-op, January – July 2010

Evaluated shake flask model for cell culture growth and enzyme production of batch-refeed process

Evaluated performance of newly purchased 12L glass bioreactors

Studied effect of temperature and pH on stability of enzyme product

Technical Skills

Skills: Cell culture (batch, fed-batch, batch-refeed continuous), cell banking, media and buffer prep, clarification and filtration, UF/DF, tech transfer, familiarity with downstream unit operations

Cell Line Experience: CHO, HEK293, DUKX B11, DG44i

Lab Equipment: NOVA, NOVA pHOx, NOVA Flex, BGA, Osmometer, Cedex BioHT, Cedex, Vi-Cell, YSI, Hemocytometer, HPLC, NanoSight NTA, Endosafe, AKTA, Glasswashers, Autoclaves, Tubing Welders, ABER, pH, DO, and pCO2 probes

Bioreactors Systems: Finesse DeltaV, Applikon glass bioreactors, Thermo SUBs (50L – 2000L), GE WAVE/XURI, ambr15, Sartorius Biostat, GE Xcellerx XDR SUB, XCell ATF

Programming Languages: C++, Visual Basic, Scheme

Software: IDBS ELN, Finesse DeltaV, Design Expert, SIMCA, JMP, SolidWorks Certified, MATLAB, Microsoft Office Suite, Seeq, Unicorn

Interests

Vintage audio, cycling, canoeing, fishing, gardening, interior design, board and card games, cooking, landscaping, yard sales

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