Scientist micro/molecular biology R&D

Location:

Irvine, CA

Salary:

80000/yr

Posted:

January 07, 2021

Contact this candidate

Resume:

Matt Earl Cruzen, Ph. D.

***********@*****.***

P.O. Box 23192

Santa Ana CA, 92711

Cell:714-***-****

RESUME

Objectives:

To be a full time life science professional, utilizing my broad background and training in molecular biology/biochemistry, communication skills, and experience. My strengths are a persistence and dedication with an inner drive towards accomplishing goals in a team environment, including a true passion for teaching and seeing others learn and excel. I also have a hunger for more knowledge and to communicate to others as a lifelong learner and team player.

Education:

Institution Degree Field of Study

California State Polytechnic University, Pomona B.S.-1985 Microbiology

University of California, Irvine Ph.D.-1993 Biological Chemistry

California State University, Long Beach Teacher credential program 1998-2000

Major Research Field: Microbiology, Genetics, and Molecular Biology.

Honors: California State Polytechnic University, Pomona: Magna Cum Lauda, 1985.

Skills and Experience Summary:

●Microbiology sterile technique

●production and evaluation of gold nanoparticle conjugates

●Assay development for microbial testing in soil, water, food

●Efficacy testing of antimicrobials

●Spectrophotometry

●Enzyme assays

●Light microscopy

●Fluorescent microscopy

●Scanning electron microscopy

●Mammalian cell tissue culture

oEstablish and maintain cell lines

oScale up batch cultures

●Virus quantification

●Flow cytometry

●FISH

●Aging studies with C.elegans

●PCR

●Gold nanoparticle conjugation for LFA

●Various chromatography methods for protein purification

●Nucleic acid and protein extraction purification

●ELISA plate and Ab preparation and quantification

●Tissue processing/embedding/sectioning and analysis

●H & E reagent prep and staining, IHC slides

●Gel electrophoresis and blotting DNA, RNA, Protein

●Biological safety officer

Research and Industry Experience:

Lead Chemist,Applied Biomedical LLC, April 2020-October 2020.

Troubleshooting and process improvement of diagnostic LFA assays for covid antibodies

manage and supervise technicians

Technical Support Scientist I, Focus Technologies Incorporated, September 2001-April 2002.

Troubleshooting of diagnostic assays

Prequalification of incoming raw materials

Design and develop methods for streamlining and reducing cost in production.

Write and present technical material in group settings

cGLP/cGMP and ISO9001 training

Microbiologist, Medical Chemical Corporation, April 2002

Develop and perform efficacy assays on batch samples of industrial cleaning products

Contract project scientist Avid Bio services April 2002-August 2002.

Monitor and optimize growth rate and product yield of transformed mammalian cell lines in bioreactors.

Collaborate with client and make reports based on experiments to maximize product yield

Research Associate, Oncotech Incorporated, June 1993 - August 1994.

Assay development, and independent research of human tumor necrosis factor (TNF) intracellular processing and biological activity.

Drug and assay development of compounds which affect glutathione metabolism in breast, ovarian, and melanoma cell lines, and fresh tumor specimens, employing experimental procedures such as ELISA and spectrophotometric enzyme activity assays and cell surface characterization by flow cytometry.

Responsible for maintaining cell culture, and laboratory inventory and ordering.

Graduate Student, University of California, Irvine, September 1985 - April 1993.

Carry out original research cloning, characterization, and molecular biology of human aminoacyl-tRNA synthetases using experimental procedures such as Southern and Northern blotting, in vitro transcription, manual and automated sequencing, and PCR.

Carry out physiology and aging studies in Caenorabditis elegans monitoring a variety of enzyme activities by fluorometric analysis of life span specified cloned nematodes.

Supervise undergraduate and rotating graduate student projects.

Administrative Experience:

Biological Sciences department chair, Biola University, Spring 2005-Spring 2017.

Coordinate faculty schedules and course loads

Working with department budgets

Provide perspective of the biological sciences on university wide issues.

Coordinate with other department chairs

Seek out and hire full and part time faculty (5 full time faculty and several adjunct instructors hired)

Development of current and new academic programs

oCurrently in the process of getting state approval for a newly developed proposal in process, clinical laboratory scientist [CLS] program with a new concentration of Biological Sciences: biological chemistry

Biological Safety Officer: Write and update Standard Operating Procedures for all laboratories involving the use and disposal of potential and known biological agents. Manage the handling of biological hazardous wastes. Train laboratory technician and teaching assistants regarding biological safety. Training and certificate Spring 2016

Director, Community College SMET programs, University of CA, Irvine Center for Educational Partnerships, September 2000 – September 2001.

Develop and implement curriculum for high school and community college transfer students in UC summer institutes.

Develop policy statements regarding the selection and participation criteria for program participants.

Work with transfer center directors and program directors of local and statewide community colleges to increase transfer rates to University of CA in the science, math engineering and technology majors.

Develop and consult on grant writing for departmental proposals.

Develop and modify detailed annual program plans, and program budgets for summer programs.

Represent and present on the behalf of UC Irvine at relevant public and professional meetings.

Teaching Experience:

Associate Professor, Biola University, La Mirada CA, August 2002 – present.

Develop and teach courses in: General Biology for majors, Cellular and Molecular Biology, Cellular Biology Laboratory, Genetics and Genetics Laboratory, Developmental Biology, and Microbiology, Immunology and Virology. Perform certain extra academic duties as required of a full time faculty member and chair including: developing an undergraduate research program [aging studies in Caenorabditis elegans], serving on the General Education Council, Course Evaluation Committee (chair), Faculty Personnel Committee, and Biological Sciences department chair Spring 2005-Spring 2017.

Microbiology Instructor, Irvine Valley College, Irvine CA, August 1994 –June 1998.

Teach microbiology lecture and laboratory. The course enrolls 32 students per semester. Responsibilities include instruction and evaluation of theory and applied skills in microbiology.

Biological Sciences Instructor, Santa Ana College, Santa Ana CA, August 1993 – June 2000.

Teach biology and microbiology laboratory and lecture. These courses enroll an average of 30 students per semester. Responsibilities include instruction and evaluation of theoretical and applied skills.

Science Teacher, Century High School, Santa Ana Unified School District, September 1997- June 2000.

Develop curriculum and teach integrated science to freshmen, sophomores and juniors.

Instructor, University of California Irvine Summer Institutes (CAMP-Community College Summer Institute, CAMP Summer Science Academy, Upward Bound, and AP Biology, July and August 1994, 1996, 2000, 2001.

Wrote curriculum for and taught activity-based courses in molecular biology, general biology, physics and chemistry to underrepresented minority community college transfer students and high school students in laboratory settings.

Graduate teaching assistant, University of California, Irvine, January 1985 to March 1988.

Taught senior level biochemistry laboratory and genetics for biology majors.

Professional Societies and Affiliations:

American Association for the Advancement of Science

American Field Service: Foreign exchange student to France 1979-1980

California Teachers Association

Publications:

Cruzen M.E. and Arfin, S.M. (1994). Nucleotide and Deduced Amino Acid Sequence of Human Cysteinyl-tRNA Synthetase. DNA Sequence-J. DNA Sequencing and Mapping 4, 243-248.

Cruzen, M.E. Characterization of cDNAs and Genomic DNAs for Human Threonyl- and Cysteinyl-tRNA Synthetase. Doctoral Dissertation. University of California, Irvine, 1993.

Cruzen M.E., Bengtsson, U., McMahon, J., Wasmuth, J., and Arfin, S.M. (1992). Assignment of the Cysteinyl-tRNA Synthetase Gene (CARS) to 11p15.5. Genomics 15, 692-693.

Arfin, S.M., Gerken, S.C., Kontis, K.J., and Cruzen, M.E. (1992). "Threonyl-tRNA Synthetase Gene Amplification in Borrelidin Resistant Mammalian Cells", Gene Amplification in Mammalian Cells: Techniques and Applications. Kellems, R.E. (ed.), Marcel Dekker, Inc. New York, pp. 97-105.

Cruzen, M.E., and Arfin, S.M. (1991). Nucleotide and Deduced Amino Acid Sequence of Human Threonyl-tRNA Synthetase Reveals Extensive Homology to the E. coli and Yeast Enzymes. J. Biol. Chem. 266, 9919-9923.

Research Presentations:

Meeting on C. elegans, Cold Spring Harbor, New York, May 6-10, 1987. Presented a poster on Lysozomal Enzyme Activity as a Biomarker of Aging Using Long-Lived Mutants of Caenorabditis elegans. Abstr. Meeting on C. elegans. p.155.

Thirteenth International tRNA Workshop, Vancouver, Canada, June 4-9, 1989. Presented a poster on Human Threonyl-tRNA synthetase: cDNA and Deduced Amino Acid Sequence. Abstr. 13th international tRNA Workshop Th-am-17.

Undergraduate Science symposium. Biola University, La Mirada CA June 2012-June 2016. Annually organize event and supervise student presentations that worked in my lab doing scanning electron microscope projects on aging Caenorhabditis elegans wild-type and age-1 mutant worms.

References:

Professor Rafe Payne

Professor Emeritus Biola University

13800 Biola aveneue

La Mirada, CA 90639

714-***-****

Dr. Carol Taylor

President Evangel University

Springfield MO

417-***-**** ext. 7200

Dr. Harvey Havoonjian

Biola University