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Assistant Engineering

Location:
Somerville, MA
Posted:
January 05, 2021

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Resume:

KRITIKA MUKHERJEE

Somerville, MA adi6v4@r.postjobfree.com 781-***-**** https://www.linkedin.com/in/kritika-mukherjee-5185a2172/ SUMMARY

Extremely devoted, passionate and well informed graduate researcher seeking for Full time positions. EDUCATION

Masters of Science in Biotechnology Anticipated December 2020 Northeastern University Boston, MA GPA 3.38

Concentration: Molecular Biotechnology

Course work: Introduction to Biotechnology, The Biotechnology Enterprise, Cell Culture Proc/Biophrm Prod, Molec Cell Biology for Biotech, Experimental Design/Biostats, Protein Chemistry, Cutting-Edge Apps - Molec Biot, Behavioural Pharmacology Drug Discovery, Application Biotechnology Lab.

Training: Autoclave PART I & II, Biosafety Part I / Biosafety Awareness Training, Fundamentals of Laboratory Safety, Hazardous Waste Management Classroom Training.

Bachelor of Engineering in Biotechnology May 2018

Thadomal Shahani Engineering College Bandra, India CGPA 7.47 SKILLS

Biotechnology Skills: Protein Extraction, Electrophoresis, DNA extraction, DNA Purification, Nano drop spectrophotometer, Cell Culture, Animal Culture, PCR, MULTIPLEX PCR, Radioactive Binding Assay, Plant culture, BLAST, FASTA, pyMOL, Agarose Gel Eletrophoresis, SDS-page, Size Exclusion Chromatography, Affinity Chromatography, Paper Chromatography, Proficiency in working with radioactive material, Western Blotting, PRISM, culturing hematopoietic stem cells. Other skills: MS office, C, Excellent observational skills, Accuracy in following procedures and keeping records, Technical skills, Critical thinking skills, Strong written and verbal communication skills, Analytical abilities, Good presentation skills, Team work, Good communication skills, Logical approach. PROJECT

Undergrad Research Project 2017-2018

• Executed a research project on Eisenia Fetida (earthworm) using different pharmacological agents by modulating the proteins. After the successful modulation of proteins they were analysed with the help of graphs. Accomplished protein extraction using

chloroform-methanol technique. Samples were given for profiling at IIT Bombay lab and orbitrap.

• Established connection between pharmacological agents and tissue regeneration for each earthworm by examining under the microscope using histology techniques.

WORK EXPERIENCE

Co-op Genome Engineering

VOR BIOPHARMA 07/2020-Present

• Cultured primary hematopoietic stem cells and THP-1 AML cell line

• Performed electroporation on THP-1 cells with GFP mRNA used as a positive control for various experiments

• Delivered Cas9 RNP complexes into hematopoietic stem cells and THP-1 cells.

• Designed primers for sequencing utilizing Benchling tools.

• Quantified DNA/RNA using the Qubit fluorometer.

• Performed Mycoplasma testing in newly thawed cell lines

• Optimized PCR conditions to select specific oligos for amplicons containing CRISPR/Cas9 induced indels.

• Performed ICE analysis using sanger sequencing to determine editing in the THP-1 cell line and hematopoetic stem cells.

• Used CRISPR Cas9 gene editing technology to knock down a protein in hematopoietic stem cells.

• Performed data analysis to estimate editing efficiency using CRISPR Cas9 tools. Graduate Research Assistant Dec2019- March

Centre for Drug Discovery (Northeastern University)

• Culturing and passing cancer cells lines (213, THP, MCF10A, U87, WBC)

• Practiced cell freezing techniques for long term preservation.

• Performed MTT cell based assay.

• Performed ELISA to confirm a protein sample

• Starve media preparation for cell harvesting.

Graduate Research Assistant July-Dec 2019

Loring’s Lab (Northeastern University)

• Cultured HEK-blue cells

• Worked on the construct of ha7-C5HT3-TMD1-3.

• Performed PCR to amplify the new insert.

• Transformed the dam-/dcm- with the new construct.

• Extracted DNA from the newly transformed cells for sequencing. Graduate Research Assistant

Kim Lewis Antimicrobial Research Centre (Northeastern University) Jan 2019-April 2019

• Worked with a Postdoc fellow in the Lewis lab focussing on investigating the molecular mechanisms of ATP- dependent persister formation using Borrelia burgdorferi - the causative agent of Lyme disease - as a model organism.

• Achieved transformation in E.coli

• Procured ligation in E.coli

• Executed electrophoresis in Borrelia

• Preparation of Borrelia media

• Successfully tested fluorescence in E.coli for Queen 2m activity.

• Conducted plasmid and DNA isolation from E.coli. Haffkine’s Institute (Trainee) 2015-06

• Qualified immunological techniques like ELISA with HRP conjugated antibody to detect an immobilized antigen.

• Cultured HELA cells in TPP flask.

• Achieved chick embryo isolation and dyed the amniotic sac.

• Implemented PCR on a short random sequence and analysed the results with SDS- Page.

• Performed gel electrophoresis with the help of geldoc.

• Accomplished Heamagglutination.

Royal Diagnostic Centre (Internship) 2017-06--2017-08

• Acquired training in routine biochemical, hematological, immunological and clinical pathology investigations.

• Practiced hands on training on sphere random access biochemistry analyser. ACOMPLISHMENTS

• 1st price in National level competition in drama at IIM Khozikhode. Jan 2018

• ‘Hall of fame’ for representing Thadomal Shahani College at National level. Mar 2018 PUBLICATION

Shivangi Modi, Kritika Mukherjee, Project on ‘Assessment of effects of the pharmacological agents in the modulation of regenerative abilities in Eisenia Fetida’ volume 5 issue 3, Paper Id V5I3-1403.



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