DEEPA RUHELA WALIA, Ph.D.

adhzhn@r.postjobfree.com, adhzhn@r.postjobfree.com

Phone: +1-443-***-****,609-***-****

Immigration Status: Permanent resident USA (Green card holder)

PROFESSIONAL STATEMENT

Research Associate Scientist in immuno-therapeutics for pain management-opioids and diabetics, stem cells with 15 years extensive biotechnology/per-clinical experience and interest.

EDUCATION

Ph. D. School of Biotechnology, Jamia Hamdard University, Delhi, India (2015).

M.Phil. Microbiology, School of Microbiology, CCS University, Meerut, India (2001).

M.S. Microbiology, CCS University, Meerut, Uttar Pradesh, India (2000).

B.S. Biology, CCS University, Meerut, Uttar Pradesh, India (1998).

WORK HISTORY

2019 July-Present: Research Associate, University of Maryland Medical School, Baltimore, USA

(Effect of Opioids-poisoning, CO-poisoning, High pressure and CO2 insult on microparticle production in blood and brain and connection with glymphatic channels).

2018 June-2019 July: Postdoctoral Fellow, University of Maryland Medical School, Baltimore, USA

(Protective effect of astrocyte Heme oxygenase-1 after intracerebral hemorrhage).

2016 Nov– 2018 May: Postdoctoral Fellow and Research Specialist, Johns Hopkins Medical School, USA

(Defining IL-4 activated monocytes as a viable cellular immunotherapy in lung cancer) (Suppression of human natural killer cells by different classes of Opioids)

2015 Feb – 2016 Feb: Postdoctoral Fellow (Research Associate), National Institute of Plant Genome research (NIPGR), India

(Studying the role of hyaluronic acid and N-Acetylglucosamine catabolic pathway in virulence of pathogens in mice model)

2010 – 2015: Doctoral thesis project (Advisor: Prof. Asis Datta)

(Thesis title: Molecular cloning and characterization of GlcNAc inducible Hexosaminidase in the human fungal pathogen Candida albicans).

FELLOWSHIPS

2012 – 2014 Senior Research fellowship by Council of Scientific and Industrial Research, India.

2007 – 2012 Senior Research fellowship by Indian Council of Medical Research, India.

2004 – 2007 Junior Research fellowship by National Institute of Plant Genome Research, India.

2002-2003 Microbiology Faculty, CCS University, Meerut, Uttar Pradesh, India.

PUBLICATIONS

1.Peng D, Chen CA, Ruhela D, Li Y, Regan RF., Deferoxamine deconditioning increases neuronal vulnerability to haemoglobin. (2020) Exp Cell Res. Feb 26, 111926. doi: 10.1016.

2. Ruhela D, Bhopale VM, Yang M, Yu K, Weintraub E, Greenblatt A, Thom SR., Blood-borne and brain-derived microparticles in morphine-induced anti-nociceptive tolerance. (2020) Brain Behav Immun. Jan 27, S0889-1591(19)31234-6.

3. Dermot P. Maher, Deepa Walia, Nicola M. Heller., Morphine decreases the function of primary human natural killer cells by both TLR4 and opioid receptor signalling. (2020) Brain Behav Immun. Jan1, 83:298-302.

4. Dermot P. Maher, Deepa Walia, Nicola M. Heller., Suppression of human natural killer cells by different classes of opioids. (2019). Anesthesia & Analgesia. 128(5):1013-1021.

5.Tuday E, Nomura Y, Ruhela D, Nakano M, Fu X, Shah A, Roman B, Yamaguchi A, An SS, Steenbergen C, Baraban JM, Berkowitz DE, Das S., Deletion of the microRNA-degrading nuclease, translin/trax, prevents pathogenic vascular stiffness. (2019) Am J Physiol Heart Circ Physiol.1;317(5):H1116-H1124.

6.Dermot P. Maher, Deepa Walia, Nicola M. Heller. Human natural killer cell activation following stimulation of cannabinoid receptors. (2020) (Manuscript submitted Brain Behav Immun).

7.Ruhela D, Bhopale VM, Yang M, Yu K, Weintraub E, Greenblatt A, Thom SR., High pressure exposure decreases plasma gelsolin concurrent with increased microparticles and IL-1β in humans and mice; gelsolin supplementation lyses particles.(2020) (Manuscript submitted JCI).

8.Deepa Ruhela, Mohan Kamthan, Paramita Saha, Subeer S Majumdar, Kasturi Datta, M.Z.Abdin, Asis Datta, In vivo role of Candida albicans β- Hexosaminidase (HEX1) in carbon scavenging. (2015) Microbiology Open 4 (5): 730–742.

9.Deepa Ruhela, K. Hanumantha Rao, Swagata Ghosh., M. Z. Abdin., Asis Datta., N- acetylglucosamine kinase, HXK1 contributes to white-opaque morphological transition in Candida albicans. (2014) Biochem Biophys Res Commun 445(1):138-144.

(Awarded first position among top 20 articles in 2104 at Global Medical Discovery).

10.Mohan Kamthan, Deepa Ruhela, Vijaya Kumar Nalla, Ayushi Kamthan., Protiti Maiti., Asis Datta., Characterization of a Putative Spindle Assembly Checkpoint Kinase Mps1, suggests its involvement in division, morphogenesis and oxidative stress tolerance in Candida albicans. (2014) Plos-One (9): 101***-******.

11. Mohan Kamthan, Ayushi Kamthan, Deepa Ruhela, Protiti Maiti, Neel Sarovar Bhavesh, Asis Datta. Upregulation of galactose metabolic pathway by N-acetylglucosamine induced endogenous synthesis of galactose in Candida albicans. (2013) Fungal Genetics and Biology. (54): 15–24.

TECHNICAL EXPERTISE

1.Immunology and cell Biology:

Clinical studies on lung cancer human patients and mouse model for IL-4 targeted immunotherapy and clinical sample management.

Developed immunoassays, cell-based assays and Macrophage migration assay to quantify the interaction between targeted immune cells and IL-4 cytokine level in Lung cancer model in human, mouse and physiological responses in cultured primary human macrophage cells and described a profile of differentially regulated inflammatory markers relevant to Lung cancer and injury.

Cultured human macrophage and polarized them with cytokines. NK cells killing assays and monocytes cytotoxic assay, IL-1B ELISA, Gelsolin, Phagocytosis assay with human macrophage.

Developed XTT, MTT and Trypan blue exclusion cell viability assays in cancer cell lines to study the effects of small molecule PLD inhibitors.

Advanced Annexin V-PI staining by Flow Cytometry to study apoptosis in lung cancer cells.

Developed hypothesis and research plan to target autophagy mechanism and cell survival signalling in Lung cancer cells to support small molecule drug discovery for cancer therapeutics.

Purification and labelling of antibodies, Antibody kinetics and titration, Antibody internalization.

Primary astrocyte, glial cell and Mammalian cell culture, Stem cell isolation, Microparticle isolation from blood and deep cervical lymph nodes. K562 cell line culture maintenance and

Phase contrast and Confocal microscopy, Immunohistochemistry.

Multi-colour Flow cytometry (Cyto-Flex-BD and MACS Quant -Miltenyi)

2. Neuroscience and animal handling:

Mice handling, breeding and dosing (IV, IP, SubQ and Oral, Intranasal)

Intracerebral haemorrhage surgery and subarachnoid haemorrhage surgery

Behavioural study after surgery and drug dosing through IP and intranasal into mice

IP and IV catheters insertion in mouse during MRI brain imaging.

Cannula Implantation into the Cisterna Magna of Mouse

Tail flick study for Morphine tolerance in Mouse

Brain, Skeletal muscle, Colon Endothelial cell isolation and capillary leak rhodamine conjugated dextran assay.

3. Genomics:

Reverse transcription and quantitative Real-Time PCR

Gene knockout and mutant preparation in microbes and mice with homologous recombination technology.

Proteomics:

Protein purification

DNA-Protein interaction studies: Promoter deletion Assay, Gel Retardation or Gel-shift Assay

Affinity purification, Ion-exchange chromatography, Gas chromatography.

Preparation of protein samples for MALDI-TOF mass spectrometry analysis.

2. Bioinformatics and lab management:

Promoter sequence analyses and transcriptional factor binding prediction analyses

Software tools: Cytoflex, Quantify, FlowJ, ImageJ, GraphPad Prism, Microsoft office.

Developed strong research collaborations across scientific teams and help brought lab grants DoD and ONR grants approx. $500k.

Managed lab equipment and inventory maintenance

Mentored and trained undergrad students in molecular biology and biochemistry research techniques. Partnered with mentor to develop grant and authored article on- Opioids based research and brain-derived microparticles.

REFERENCES

1.Prof. Nicola M. Heller, Associate Professor

Johns Hopkins Medical School Baltimore, 21218, USA

Email: adhzhn@r.postjobfree.com Ph: 955-***-****

2. Prof. Dermot Maher, Assistant Professor and Clinician

Johns Hopkins Medical School Baltimore, 21218, USA

Email: adhzhn@r.postjobfree.com Ph: 955-***-****

3. Prof. Samarjit Das, Assistant Professor

Johns Hopkins Medical School Baltimore, 21218, USA

Email: adhzhn@r.postjobfree.com Ph: 443-***-****

4. Prof. Asis Datta, Professor of Eminence (Ph.D. thesis advisor)

National Institute of Plant Genome Research, New Delhi 110067, India.

Email: adhzhn@r.postjobfree.com Phone: +91-11-267*****, Fax: +91-11- 26741

5. Prof. Kasturi Datta (Professor at Indian National Academy of Sciences)

School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India,

E-mail: adhzhn@r.postjobfree.com Phone: +91-11- 26704327.

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