Killian F. McDonald
**** ******** ***** **., *** FOREST, IL 60452 708-***-**** adhw99@r.postjobfree.com
Research Experience
Toxicology Research at UIC (M.S. of Forensics) 2020-2021
Responsible for developing and validating an extraction and detection protocol for novel blood doping compounds utilizing LC-MS/MS QqQ
Conduct limit of detection, specificity and robustness studies
Perform the necessary maintenance and troubleshooting of LC-MS/MS QqQ instruments
Validate the purchased controls and negative control material
Prepare the solutions and reagents necessary for extraction procedures
Formulate controls and internal standards for analysis
Interpret the data and spectra from the analyses to determine the next steps in the experimental process
Work Experience
Simple Laboratories Molecular Biology Department - Harwood Heights, IL
Laboratory Technician II –July 2020-Current
Serve as hands-on technician performing molecular biology assays, such as RT-PCR
Conduct MagMax96 machinery for automated transfer and processing
Prepare wash and elution plates for PCR plating, as well as reagents necessary for analysis
Define and assign samples, targets, and tasks for PCR analysis using the QuantStudio software
Review and provide analysis of clinical data and prepare reports in accordance with government and company regulations
Serve as a client services representative to handle patient data and coordinate results with medical personnel
Monitor the RT-PCR process and perform quality control
QA all of the SARS-Cov-2 swabs prior to analysis
Inspect instruments prior to use, including required maintenance checks
Maintain department inventory sheets and daily specimen tracking lists
United States Drug Testing Laboratory (USDTL) - Des Plaines, IL
Laboratory Technician I – June 2019-July 2020
Conduct chemical extractions on biological fluids, blood spots and tissues within a forensic drug toxicology laboratory environment
Utilize various extraction techniques such a s solid phase (SPE) and liquid-liquid extractions to prepare samples for alcohol/drug screening and confirmation testing
Utilize safety and quality control procedures to ensure accurate results, accountability and integrity of chain of custody of sample material in accordance with company policy.
Average monthly productivity of 1800 samples; <2% error rate
Other laboratory extraction related activities include: validation of purchased controls, negative control material, method validations, research studies and instrumental test performance
Prepare solutions, reagents, or sample formulations needed for laboratory work and extraction procedures
Education
University of Illinois at Chicago- Chicago, Illinois
Master of Science in Forensic Science, concentration in Toxicology (May 2021)
Loyola University Chicago – Chicago, Illinois
Bachelor of Science in Forensic Science & Minor in Spanish Literature (May 2019)
Professional Organizations
American Academy of Forensic Sciences
Chicago Chromatography Discussion Group
Society of Forensic Toxicologists
Language Skills
Ability to speak, read and write fluently in Spanish
Professional Skills
LC-MS/MS QqQ
Agilent 7890A/5975C GC-MSD
PerkinElmer Spectrum 100 FT-IR-ATR
Applied Biosystems Quantstudio 5 and 12k Flex
Hamilton Microlab Star Liquid Handler
UV-Vis spectrophotometer
Solid Phase Extraction UCT Columns
Also familiar with DNA IQTM by Promega, Quantifiler Trio using TaqMan probe, Applied Biosystems 7500 Real Time PCR System, Applied Biosystems Veriti 96 Well Thermal Cycler, and Applied Biosystems Hitachi 3500 Genetic Analyzer