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Curriculum Vitae

Hai Li, Ph.D., Instructor

Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, MSB-6.402, 6431 Fannin Street, Houston, TX 77030, USA

757-***-****; adhokm@r.postjobfree.com

OBJECTIVE STATEMENT

As an experienced protein chemist with a great passion for biopharmaceutical research, I am seeking the position of Research Scientist with my extensive experience and skills in protein science.

EDUCATION

2011, Ph.D., Biochemistry, Old Dominion University, Norfolk, VA 2006, M.S., Biology Indiana State University, Terre Haute, IN 2004, M.S., Biology Nanjing University, Nanjing, China 2001, B.S., Biochemistry Central China Normal University, Wuhan, China PROFESSIONAL TRAINING

2018-present Instructor McGovern Medical School at UTHealth, Houston, TX 2012-2018 Postdoctoral Research Fellow McGovern Medical School at UTHealth 2012-Present Determine the X-ray crystal structures of membrane proteins (using microbial rhodopsins as target); Investigate the structure, function, and molecular mechanism of microbial rhodopsins using biochemical and biophysical approaches

Projects

• 1: determine the X-ray crystal structures of natural anion channelrhodopsins (ACRs)

• 2: determine the and EM and X-ray crystal structures of novel natural ACRs

• 3: study the in vitro activity in liposomes of ACRs

• 4: study the photochemistry including photochemical reaction cycle of ACRs

• 5: study the in vitro activity in liposomes of sodium-pumping rhodopsins Technologies

• Grow membrane protein crystals using lipid cubic phase method

• Reconstitute integral membrane proteins into liposomes

• Express membrane proteins using Pichia pastoris and insect cell/baculovirus system

• Generate stable Pichia clones expressing wild-type and mutants proteins

• Purify membrane proteins using affinity, anion exchange, and size exclusion chromatography and AKTA

• Measure the absorption spectra of rhodopsins using spectrometer and micro-plate reader

• Measure the fluorescence spectra of reconstituted proteoliposome with fluorometer

• Measure the time-resolved absorption change of rhodopsins using flash photolysis

• Process the data of equilibrium and kinetics using program including Origin and pClampfit

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• Harvest protein crystals and determine protein structures using X-ray diffraction

• Write the manuscripts papers for the peer-reviewed journals and abstracts for conferences

Achievements

• determined the crystal structure of an ACR at 2.9 A and determine the molecular mechanism

• determined the in vitro activities of microbial rhodopsins as pumps and channels

• Published >10 papers in high impact journals

• Collaborated with colleagues in the field by sending protein samples for characteriazation

2006-2011 Graduate Research Assistant Old Dominion University, Norfolk, VA 2006-2011 Investigated the bioinformatics, stability, structure, and folding of small soluble proteins (using death domain protein as target) with biochemical and biophysical techniques including fluorescence and CD spectroscopy, stopped- flow, hydrogen-deuterium exchange, quenched-flow, and NMR spectroscopy; Studied the structure and function of chitinase insertion domain by bioinformatics

Technologies

• Identify the conserved residues for structure, function, and stability with bioinformatics

• Express proteins in the heterologous system E. coli

• Purify proteins with chromatography (affinity, ion exchange, size exclusion) with FPLC (AKTA)

• Characterize the protein purity and quality with electrophoresis and western-blot

• Measure the equilibrium fluorescence spectra with fluorometer and kinetics with stopped-flow

• Measure the equilibrium far- and near-UV CD spectra with polarizer and kinetics with stopped-flow

• Perform the 1D- (H, C) and 2D- (HSQC) NMR measurement of protein with NMR spectrometer

• Discover the chitinase insertion domain as a new protein domain by bioinformatics tools

2004-2006 Graduate Research Assistant Indiana State University, Terre Haute, IN Investigated the effects of temperature on ammonium and ergosterol production and chitinase activity of rural and urban isolates of fungal species 2001-2004 Graduate Research Assistant Nanjing University, Nanjing, China Separated and purified novel natural products produced by endophytic microorganisms and investigated their structures and biological properties Technologies

• Extract and purify small chemical compounds as natural products from endophytic fungi

• Test the antibacterial, antifungal, and antitumor activities of the natural products 3

HONORS AND AWARDS

1998-2000 Excellent Student Scholarship Award, Central China Normal University 2005-2006 Graduate Student Scholarship, Indiana State University 2006-2009 Dominion Scholarship Award, Old Dominion University 2009-2010 University Fellowship Award, Old Dominion University 2014 Poster of Excellence Award in 37th Meeting of American Society Photobiology 2016 4th Place in Poster Presentation in Biochemistry Program Retreat, UTHealth 2017 1st Place in Poster Presentation in Biochemistry Program Retreat, UTHealth 2018 Travel Award and invited speaker at 18th International Conference Retinal Proteins

SELECTED PUBLICATIONS (1st author)

1. Li, H., Huang, C.Y., Govorunova, E., Schafer, C., Sineshchekov, O., Wang, M., Zheng, L., Spudich, J. Crystal structure of a natural light-gated anion channelrhodopsin. eLife,41741, 2019.

2. Li, H., Sineshchekov, O.A., Wu, G., and Spudich, J.L.: In vitro activity of a purified natural anion channelrhodopsin. J. Biol. Chem., 291:253**-*****, 2016. 3. Li, H., Sineshchekov, O.A., da Silva, G.F.Z., and Spudich, J.L.: In vitro demonstration of dual light-driven Na+/H+ pumping by a microbial rhodopsin. Biophys. J., 109:1446-1453, 2015.

4. Li, H., Govorunova, E.G., Sineshchekov, O.A., and Spudich, J.L.: Role of a helix B lysine residue in the photoactive site in channelrhodopsins. Biophys. J., 106:1607-1617, 2014. 5. Greene, L.H.§, Li, H.§, Zhong, J., Zhao, G., and Wilson, K.: Folding of an all-helical Greek- key protein monitored by quenched-flow hydrogen-deuterium exchange and NMR spectroscopy. Euro. Biophys. J., 41:41-51, 2012. (§:co-first author). 6. Li, H. and Greene, L.H.: Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding. PLoS One, 5:e8654, 2010.

7. Li, H., Wojtaszek, J.L., and Greene, L.H.: Analysis of conservation in the Fas associated death domain protein and the importance of conserved tryptophans in structure, stability and folding. Biochim. Biophys. Acta (BBA) - Proteins and Proteom., 1794:583-593, 2009. INVITED TALKS

1. Hai Li, Oleg A. Sineshchekov, Giordano F. Z. da Silva, and John L. Spudich. In vitro demonstration of light-driven Na+ /H+ pumping by a microbial rhodopsin. Gordon Research Conference on Photosensory Receptors & Signal Transduction, Galveston, TX, January 24- 29, 2016.

2. Hai Li, Elena G. Govorunova, Oleg A. Sineshchekov, and John L. Spudich. Role of a helix B lysine residue in the photoactive site in channelrhodopsins. American Society for Photobiology 37th Conference, San Diego, CA, USA, June 14-19, 2014. 3. Hai Li, Lesley H. Greene. Monitoring the folding of Fas-associated death domain protein by pulsed hydrogen-deuterium exchange combined with NMR spectroscopy. Biophysical Society 55th Annual Meeting, Baltimore, MD, USA, March 5-9, 2011. 4. Hai Li, Meghan Woughter, Jessica Wojtaszek, Lesley Greene. The importance of conserved hydrophobic core residues on the structure, stability and folding of Fas- associated death domain protein. 23rd Symposium of the Protein Society, Boston, MA, USA, July 24-29, 2009.

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5. Hai Li, Lesley Greene. Proposed role of the chitinase insertion domain in chitinase function. 235th ACS National Meeting, New Orleans, LA, USA, April 6-10, 2008. 6. Hai Li, Lesley Greene. Investigating the folding of a death domain protein and hydrophobic core formation. 235th ACS National Meeting, New Orleans, LA, USA, April 6-10, 2008.

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