Ritika Singh

Email: adhjvy@r.postjobfree.com

Phone no. +44-079********

Glasgow, G1 1HE

Summary. A postgraduate in pharmacology with a background in biotechnology having expertise in various laboratory techniques and enthusiastic about contributing to the healthcare industry applying for the position of Lab technician. Educational Qualifications

University of Strathclyde

MSc Advanced Pharmacology 55% 2019-20

Thesis title- N terminal proteolysis decides the ending. While the N terminal is important for GPCR signaling absence of it can deactivate the receptor and further inactivates its constitutive property. Proteolysis is important for post translational modifications like transcription, cell differentiation, cell death. There are proteases cleaving a specific site of the N terminal to activate a specific G protein coupled receptor to stimulate a signaling process for example – thrombin cleaving a specific site at protease activated receptor (PAR1) to stimulate a biological reaction known as blood coagulation cascade, on the other hand, some G protein coupled receptors don’t require proteases or peptidases to undergo proteolysis, their N terminal act as an intramolecular ligand or as an intramolecular agonist. By blocking and enhancing N terminal signaling, therapeutics can be designed for prevention of diseases caused by modulation of proteolysis at the N terminal.

Certification

PIL A/K- Animal handling

Species specific: Rats and mice

Dr. APJ Abdul Kalam Technical University

Bachelor’s in Technology Biotechnology 61% 2015-19 Thesis title - Dye effluents discharged from textile industries are responsible for water pollution. Considering the discharge produced in the terms of composition and volume, it was named one of the major sources of wastewater in the world. Therefore, it is important to treat these water bodies as effectively as possible which can be done by using bio sorbents because they are cheap, widely available and environment friendly. Using activated carbon to purify wastewater containing dye to make it clean to be discharged into river bodies. Work Experience

Department of forensic medical and toxicological studies June 2018-August 2018

Muzaffarnagar medical college, Muzaffarnagar, India. o Collection and preservation of forensic evidence like hair, fiber, blood stains, seminal stains, and toxicology sample.

o Identification and comparison of hair, blood stain, fiber. o Difference between hair and fiber.

o Examination of blood and seminal stains, collection, preservation and forwarding of DNA sample.

Defense institute of physiology and applied sciences, DRDO, New Delhi, India June 2017-August 2017

o Reagents and buffer preparation, protein estimation by Lowry and Bradford methods, western blotting.

o Biochemical estimation of GSH reduced glutathione, free radical catalase activity in skin tissue and blood samples of exposed rats, exposure of rats in anaerobic radio frequency chamber.

o Fluroro-Jade staining and behavior of rats.

Laboratory techniques and skills

Molecular biology techniques

o Gel electrophoresis

o Polymerase chase reaction

o Western blotting

Immunology techniques

o Immunoblotting

o Immunohistoblotting

o ELISA

o Antibody generation

o Antibody isolation and Purification

o Immunoprecipitation

Microbiology techniques

o Cell culture techniques

Skills

o MS-EXCEL

o Big data analysis

o Codon code

o ImageJ

o Searching and reviewing literature for study

Volunteer experience and extra curriculars

o Working as a child befriender with Govan help, Glasgow, since November 2019. o Worked as a waiting staff at the ferry, Glasgow from November 2019-February 2020. o Participated in technical festival at IMS Engineering College, Ghaziabad, India while working on project – Smart dustbin.

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