Nitin Supekar. Ph.D.

Sr. Research Associate (Analytical Services and Tranining Laboratory, CCRC, Unviersity of Georgia, Athens) Phone# 706-***-**** Email – adgrba@r.postjobfree.com; adgrba@r.postjobfree.com Experience

Research Professional II - Sr. Research Associate (Analytical Services and training laboratory, Complex Carbohydrate Research Center, University of Georgia, Athens, USA.) 2016 - Present Functioning as a core team member and intellectual contributor for analytical method development for characterization of glycopeptides, monoclonal antibodies (mAb) & glycoproteins by Mass Spectrometry. Developed analytical methods to study the mutations and post translational modifications (PTMs) such as glycosylation on glycoproteins/(mAb) and their effect on its biological functions for preclinical process development using proteomics and glycoproteomics analysis via peptide mapping. Undertaking more than 10 analytical method development projects of glycan, glycoprotein/protein characterization using mass spectroscopy from clinical, academia and industries (mostly confidential and FDA regulatory/GLP compliant analysis) through collaborations and services. Provides training and lectures on mAb, large molecules, glycan, glycoprotein analysis to international scientists and researchers at analytical services and training laboratory.

Graduate Research Assistant (Complex Carbohydrate Research Center, University of Georgia) 2010 – 2016 Extensive experience in development, HPLC purification, MS characterization and immunological evaluation of carbohydrate-based cancer vaccine candidates including unglycosylated and heavily glycosylated glycol- lipopeptides .Studied anticancer properties synthetic glycolipopeptides alone as well as in combination with check point inhibitors such as anti-PD1 & anti-CTLA4 in mouse model using liposomal formulations. Headed number of collaborative research projects involving multiple universities across the world including United States. Key words: mAb, glycans, glycoproteins, PTMs, LC-MS/MS, ESI-MS, peptide mapping, proteomics, Glycomics, Protein primary structure analysis, Orbitrap, Quadrupole, Q-TOF, PD-L1, HER2, EpCAM, CPIs, HPLC, UPLC, SPR, SOP, Empower, Byonic, Proteome Discoverer Academic Credentials

Ph.D. (2010-2016) [Chemical Biology] Complex Carbohydrate Research Center, University of Georgia, Athens.

M.S. Chemistry (2005-2007); Ist Division, University of Pune, India Credentials and achievements

Worked on NIH, USA funded R21 grant on the development of tools for glycoprotein characterization (Under the process of US patenting and commercialization).

Functioning as a key group member in analytical method development projects for mAb, large molecules, glycans and glycoprotein characterization and analysis.

Developed mass spectrometric methods for identification and quantitation of therapeutic monoclonal antibodies using LC-MS, ESI-MS and peptide mapping techniques.

Designed experiments, generated and interpreted data for therapeutic protein characterization.

Conducted experiments using protein chemistry and characterization techniques including mass spectroscopy, separation assays for characterizing protein primary structure and identifying protein post translational modifications, comparability and structure elucidation in support of cell line development & process.

Developed methods for binding affinity and specificity of biomolecules to target by SPR using Biacore instrument.

Provides hands-on training and lectures to international scientists and researchers on protein and glycoprotein characterization at analytical services & training laboratory, Complex Carbohydrate Research Center.

Managed and successfully excuted external collaborations through scientific and strategic understanding. Nitin Supekar. Ph.D.

Sr. Research Associate (Analytical Services and Tranining Laboratory, CCRC, Unviersity of Georgia, Athens) Phone# 706-***-**** Email – adgrba@r.postjobfree.com; adgrba@r.postjobfree.com

Extensive experience with analytical instrumentation including maintainace and troubleshooting.

Strong written, (SOPs, reports, manuscript writing etc,.) and verbal communication and organization skills.

Mentored and trained postdoctoral researchers, Ph. D., graduate and undergraduate students.

President of University of Georgia’s Indian Student Association (2013 to 2014).

Received Certificate in Global Leadership by Office of International Education, UGA ((2013-2014)..

Participated and presented poster in Annual Meeting of the SFG on Glycobiology, Phoenix, Arizona, USA. Nov. 2019). Thesis and selected Projects

Development of novel tools for the glycoprotein and mAb characterization by Mass Spectrosopy. (NIH funded and under the process of US patenting and commercialization).

Analytical method development for primary structure characterization (sequence) and glycosylation analysis of PD-L1, EpCAM, HER2, and HER2-Fusion protein through Mass spectrometry via peptide mapping.

Mass Spectrometric (LC-MS/MS) method development for protein sequence characterization and glycosylation analysis of native and mutated large biomass degrading glycoproteins (Cel7A) derived from bacterial and fungal sources. [Collaboration with DOE’s National Renewable Energy Laboratory (NREL), Colorado.]

Development, Characterization and Immunological Evaluation of MUC1 Glycopeptide-Based Multicomponent Cancer Vaccine Candidates. [PhD Thesis]

Proficiency in Techniques, Instruments and Hands-On Expertise Instruments and techniques : Mass Spectrometry (Orbitrap Fusion Tribrid, Q-TOF, Q-Exactive, LTQ-Orbitrap Elite/Velos/Discovery, MALDI-TOF-TOF), SPR (Biacore), NMR(Bruker/Varian 500/300 MHz), UPLC (Waters Acquity), HPLC (Agilent/Shimadzu), GC-MS (Agilent), CE-MS

(Beckman Coulter PA800), CEM-Liberty peptide synthesizer, spectrophotometers, SDS-PAGE, Western blot.

Software packages : Proteome Discoverer 1.4, Byonic, xcalibur, Mascot Server, SEQUEST, Peaks, Empower, ChemBioOffice, Mnova for NMR, PyMol, Autodock, Prism, MS Office, MS Project Training programs and workshops attended

‘Hands-on’ training: Orbitrap Fusion, Q-Exactive, Orbitrap Fusion Lumos Tribrid, LTQ-Orbitrap velos mass spectrometry (Thermo Fisher); MALDI-TOF-TOF 4800 (AB life Sciences); CE-MS (Beckman Coulter PA800); SPR

(Biacore) techniques; UPLC (waters); Varian 500 MHz, Bruker Avans 300 NMR (Bruker).

The Georgia Glycoscience Symposium & CFG Workshop "Paradigms for Glycan Action in Development and Disease”– University of Georgia, USA (2 days; March, 2011).

CEM- Liberty automated peptide synthesizer service and training workshop - Greenville, South Carolina. (Sept.2011).

Certification in “Separation and Characterization of Glycoprotein and Glycolipid Oligosaccharides.” at Complex Carbohydrate Research Center Analytical Services, UGA (1 week, August, 2014). Nitin Supekar. Ph.D.

Sr. Research Associate (Analytical Services and Tranining Laboratory, CCRC, Unviersity of Georgia, Athens) Phone# 706-***-**** Email – adgrba@r.postjobfree.com; adgrba@r.postjobfree.com Publications

Chung, S.N, K.B, H.N, R.J, Y.J, B.R, Y.J, Supekar N, Vander Wall.T, “Glycosylation is vital for industrial performance of hyper-active cellulases.” ACS Sustainable Chemistry & Engineering 2019, 7 (5), 4792.

Pan H, Colville M, Supekar N, Paszek M et al. “Sequence-Specific Mucins for Glycocalyx Engineering.” ACS synthetic biology 2019, 8 (10), 2315-2326.

Ge Y, G.M, Z.M, L.J, A.J, L.W, Supekar N, Azadi. “Regulating colonic dendritic cells by commensal glycosylated large surface layer proteinA to sustain gut homeostasis against pathogenic inflammation”Mucosal Immunology 2019,1-13.

Singh, Her, Supekar N, Brooks et al. “Role of glycosylation on the ensemble of conformations in the MUC1 immunodominant epitope.” Journal of Peptide Science 2019, 5-12.

Marcelo, Supekar N, Villet et al. “Identification of a secondary binding site in human macrophage galactose-type lectin by microarray studies: Implications for the molecular recognition of its ligands.” Journal of Biological Chemistry 2019, 294 (4), 1300-1311.

N. Supekar, Boons et al. “Synthesis and Immunological Evaluation of a Multicomponent Cancer Vaccine Candidate Containing a Long MUC1 Glycopeptide.” ChemBioChem 2018 19 (2), 121.

Shajahan, Supekar N, Azadi et al.“High-Throughput Automated Micro-permethylation for Glycan Structure Analysis.” Analytical chemistry 2019, 91 (2), 1237-1240.

Shajahan, N. Supekar, Azadi et al. “Tool for Rapid Analysis of Glycopeptide by Permethylation via One-Pot Site Mapping and Glycan Analysis.” Anal Chem. 2017 89(20):107**-*****.

Amore, Knott, N. Supekar, M.Himmel, S.Decker, G.Beckham, L.Taylor et al. “Distinct roles of N- and O-glycans in cellulase activity and stability” Proceedings of the National Academy of Sciences (PNAS) 2017, 114, 52 13667.

Martínez-Sáez, N. Supekar, Peregrina et al. “Synthesis, Conformational Analysis and Biological Evaluation of an Antitumor Vaccine Derived from a Non-natural MUC1 Fragment.” Chemical Science 2016, 7, 2294-230

Lakshminarayanan, Supekar N, Boons, G.-J, Gendler et al. “MUC1 Vaccines Comprised of Glycosylated or Non- Glycosylated Peptides or Tumor-Derived MUC1, Can Circumvent Immunoediting to Control Tumor Growth in MUC1 Transgenic Mice”. PLoS ONE 2016, 11 (1), e0145920.

Thompson, Lakshminarayanan, N. Supekar, Boons et al. “Linear synthesis and immunological properties of a fully synthetic vaccine candidate containing a sialylated MUC1 glycopeptide.” Chem. Commun., 2015, 51, 102**-*****.

Abdel, Lakshminarayanan, Thompson, N. Supekar, Boons et al. “Immune and Anticancer Responses Elicited by Fully Synthetic Aberrantly Glycosylated MUC1 Tripartite Vaccines Modified by TLR2/TLR9 Agonist.”Chembiochem.2014 15(10):1508

Movahedin, Brooks, N. Supekar, Brooks et al. “Glycosylation of MUC1 influences the binding of a therapeutic antibody by altering the conformational equilibrium of the antigen.” Glycobiology 2017 27 (7), 677-687

Rios, P, C.T, M.T, C.P, L.M, P.M, Supekar N, Davis et al. “Synthetic Receptors for the High-Affinity Recognition of O- GlcNAc Derivatives”. Angewandte Chemie International Edition 2016, 55(10):3387-92. Scientific Posters at selected conferences

“Multistage enrichment strategy for sensitive and unambiguous detection of unnatural glycans by both glycoproteomics and glycomics.” N. Supekar; J. Kohler, P. Azadi et al. Poster, Annual Meeting of the SFG on Glycobiology, Phoenix, Arizona, USA. (2-5, November 2019).

Presented poster and talk on “High throughput comprehensive analysis of glycoproteins through Tool for Rapid Analysis of glycopeptide by Permethylation (TRAP) method.” N. Supekar, P. Azadi et al. Georgia Glycoscience Symposium 2018, Atlanta, Georgia, USA. April, 2018.

Development and immunological evaluation of mono-& pentaglycosylated MUC1 vaccines for cancer. N. Supekar, G.J. Boons et al. Poster presentation Pacifichem 2015. Honolulu, Hawaii, USA December, 2015.

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