CURRICULUM VITAE

Sundarapu Naga Appalaraju

Senior research fellow – NIMS

Yellamanchili, Visakhapatnam, Andhra Pradesh, (India) adgnud@r.postjobfree.com Contact number +919*********

Professional Summary: As a senior research fellow with more than 3years of experience in microbiology with a strong focus on surveillance of antimicrobial resistance, antimicrobial peptides discovery and microbial taxonomy. Education:

Master of Science in Biochemistry

Central University of Rajasthan - Ajmer, Rajasthan (2013 to 2015) Bachelors in Biochemistry

Andhra University -Visakhapatnam, Andhra Pradesh, (2009 to 2011) Work Experience:

Senior research fellow

Nizam Institute of Medical Science and Research Institute (NIMS) - Hyderabad, Telangana August 2018 to Present Dr.Sukanya Vimala (PI)

Project title: “Regional Center for Antimicrobial Resistance Surveillance Network” Objectives:

• In vitro study of antimicrobial resistance in clinical isolates of a member of the family of Enterobacteriaceae, salmonellae, staphylococci and enterococci species

• In vitro study of antifungal resistance of molds like Candida, Cryptococcus species, Aspergillus species, and zygomycetes Isolated from a sterile site of the patient.

• Phenotypic characterization of antimicrobial resistance in drug- resistant isolates. Surveillance of antimicrobial resistance in environmental isolates.

• Conduct internal quality control and participate in External Quality Assurance Services in strain identification and antimicrobial susceptibility conducted by Nodal Center assigned by ICMR. Research associate

Teena Bio-labs Pvt.Ltd-Hyderabad, Telangana April 2018 to August 2018 Objectives:

• Performing Microbial limiting testing (MLT), Pathogen testing, Bacterial Endotoxin Testing (BET) of industrial products like raw materials, water

• Performing environmental monitoring, documenting and trending results.

• Testing an industrial product with the help of Liquid-Borne particle counting.

• Laboratory equipment qualification/calibration.

Research Fellow

National Institute of Animal Biotechnology (DBT-NIAB)-Hyderabad, Hyderabad, Telangana June 2017 to March 2018

Dr.Vasundhra Bhandari (PI)

Project Title “Characterization of transglycosylases associated with cell wall biogenesis in vancomycin-resistant Staphylococcus aureus”

Objectives:

• Field sampling, isolation, and screening for Vancomycin-resistant staphylococcus aureus from bovine mastitis cow milk.

• Genotypic identification of vancomycin-resistant Staphylococcus aureus and sample preparation for MLST Sequencing.

• Study the Intracellular Staphylococcus aureus virulence in bovine mastitis cow milk using macrophages invasion assay.

• Conducted in-vitro antimicrobial susceptibility test using broth micro dilution assay moreover we also performed Mec-A gene PCR typing and Biofilm assay of vancomycin-resistant staphylococcus aureus strains.

Research Fellow

Institute of Microbial Technology (CSIR-IMTECH) - Chandigarh, Chandigarh May 2015 to March 2017 Dr. Suresh Korpolae (PI)

Project Title: “Expansion and modernization of microbial Type culture collection and Gene Bank” Objectives:

• The main objectives of this project are to work on depository, to supply authentic microbial cultures and to provide related services to the scientists working in research institutions, universities, and industries.

• Regular identification of bacteria and yeast is carried out on the basis of morphological, biochemical physiological and genotypic characteristics.

• Study of Archaeal diversity from saline habitats and screening for industrially important strains using genomics approaches.

• Bacterial systematic of phototrophs and chemotrophs.

• Focused on isolation and characterization of novel antimicrobial peptides from the diversity of complex ecosystems.

Technical Expertise:

• Knowledge of microbial taxonomy, medical bacteriology, mycology, laboratory safety, aseptic technique, a handling of pathogenic bacteria, fungal isolates, culture stock preservation and maintenance. Molecular and phenotypic Species identification using Biochemical,VITEK 2,16S rRNA &18S rRNA analysis and chemo taxonomy,NCBI-BLAST,Ez-Taxon.

• Isolation of genetic material (DNA), Polymerase chain reaction (PCR), DNA extraction, Sequencing PCR, molecular detection of Resistance gene in bacterial and fungal stains.

• In-vitro antimicrobial susceptibility testing using Broth Micro Dilution assay/PCR/Disk diffusion and VITEK 2 Test.D-Test, Vancomycin screen agar, E-strip, Performing carbapenemase detection test for the following isolates Escherichia coli, pseudomonas spp, Klebsiella spp by using carbapenemase modified hodge test,carbapenemase-imipenem-EDTA test and ESBL test.

• Biofilim assay for the Methicillin-resistant Staphylococcus aureus (MRSA strains) and other bacterial and candida species.

• Broth Micro Dilution assay of Candida, moulds dermatophytes, etc.

• Experienced in laboratory machines such as compound microscope, fluorescent microscope,MALDI-TOF and Gas chromatography, Gel filtration chromatography (G-50), HPLC, affinity chromatography, Thin layer chromatography (TLC), UV and Visible spectroscopy,PCR, Agarose Electrophoresis, protein analysis by SDS PAGES, In-Gel activity assay). Rotovap peptide extraction, AKTA pure protein purification system, Protein Estimation (BCA assay), PH meter.

• Maintaining animal cell culture like macrophage raw 264.7, J774 cell line, knowledge on Hemocytometer, and having experimental knowledge in macrophages invasion assay.

• Good communication skills in verbal and written English, Good computer skills (MS-Word, Excel, PowerPoint)

Publication:

Brahma, U., Sharma, P., Murthy, S. et al. Decreased expression of femXAB genes and fnbp mediated biofilm pathways in OS-MRSA clinical isolates. Sci Rep 9, 16028 (2019).https://doi.org/10.1038/s41598-019-52557-z November 2019

References:

Dr.Chandi C.Mandal

Associate Professor

Head Department of Biochemistry Central University of Rajasthan Email: adgnud@r.postjobfree.com

Dr.Suresh Korpolae

Senior Scientist

CSIR- Institute of Microbial Technology Chandigarh, India (160036) Email: adgnud@r.postjobfree.com

Contact this candidate