Research Scientist

Alysia Ryan

*** * **. ***. *** 724-***-****

San Diego, CA 92101 *************@*****.***

EDUCATION

Edinboro University of Pennsylvania

Bachelor of Science in Biology December 2016

Minor: Chemistry

SKILLS & ABILITIES

● Aseptic techniques

● Media preparation

● Cell culture/ Tissue culture

● Worked in an ISO and FDA certified lab

● Knowledge of LIS

● Experienced in GLP, GCP, and GCLP

facilities

● Knowledge of Good Documentation

Practices

EXPERIENCE

Inovio Pharmaceutical, Research Associate II January 2020 - Present

● Responsible for whole blood processing and PBMC isolation

● Responsible for shipping and receiving clinical samples

● Performed EliSpot assay on multiple clinical trials

● Conducted ELISA development and qualification for SARS-CoV-2 (2019-nCoV) Spike Protein under supervision of a senior scientist

● Performed ELISA testing on for COVID-19 vaccine Phase I Clinical trials

● Trained new employees in laboratory processes

● Written and updated SOPs and other controlled documents

● Maintained a complete and up-to-date list of sample inventory Q Squared Solutions, ELISA Lead Technician July 2019 - January 2020

● Oversaw the daily operation of the ELISA team

● Performed ELISAs for various vaccine trials (EBOV, HPV, Norovirus)

● Performed multiplex assay utilizing the Luminex

● Created and maintained a daily operations schedule

● Maintained an up-to-date pending log of all sample testing for multiple assays

● Responsible for the onboarding and training of new operators

● Implemented new organization and 6S procedures within the team

● Responsible for updating and ordering for supplies and reagents

● Performed reagent qualification and validation for new testing

● Assisted in new assay validation and qualification Q Squared Solutions, Laboratory Associate I/II April 2017 - July 2019

● Performed mammalian cell seeding

● Generated worklist for sample testing

● Created and maintained a detailed list of sample testing

● Responsible for training new operators on site and abroad on various laboratory procedures

● Performed the IQ/OQ/PQ process on new equipment

● Performed quality control and preventative maintenance procedures

● Performed specimen labeling, handling, preservation, processing, and storing

● Performed neutralization assays with a variety of viral antigens

● Handled and prepared media and chemical reagents

● Processed data generated from sample testing

24 Hour Fitness, Kids Club Attendant March 2017 - May 2018

● Supervised children between the age of 6 months to 12 years

● Created a welcoming environment in both the gym and kids club

● Assisted in hourly team cleans of the facilities Camp Turner, Camp Counselor June 2013 -August 2015

● Acted as the primary caregiver to children between the age of 6-16 years

● Maintained a structured schedule for campers to follow

● Created and implemented indoor and outdoor activities for campers

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