Joanna Garefalaki

Personal information:

Date of birth: March 1, 1990

Nationality: Greek / American (dual Nationality)

Gender: Female

(693-***-****

adfq07@r.postjobfree.com

linkedin.com/in/joanna-

garefalaki-02380a5b

Experience

March 2020-present

Research Collaborator

Molecular Microbiology/Immunology Laboratory (Micromol), Department of Biological Sciences, University of West Attica

February 2020-

present

Account and Sales Manager

Medical Gate Ltd.

December 2019-

February 2020

Laboratory Quality Assurance / Quality Control Assistant Alfa Medical S.A.

October 2019-

November 2020

Bioinformatician

Institute of Computer Science-Foundation for Research and Technology Hellas - FORTH

(ICS-FORTH)

Education

2017-2019

MSc. Student in Graduate program of Molecular Biology and Plant Biotechnology

(Current grade: 9.41/10)

Department of Biology, University of Crete and Institutes of Molecular Biology/ Biotechnology and Computer Science - FORTH

2013-2017 Bachelor Degree in Biology

(Grade: 7.04/10)

Department of Biology, University of Patras, Greece 2009-2013 Bachelor Degree in Biomedical Laboratory Sciences

(Grade: 7.2/10)

Department of Medical Laboratories, University of West Attica Graduate Research

& Training

October 2018-

September 2019

MSc. Candidate, Graduate Student Researcher ICS-FORTH Master thesis in “Population Genetics and Bioinformatics” Laboratory (EvoLab) at Institute of Computer Science-FORTH (ICS-FORTH).

Group leader: Pavlos Pavlidis

Master Thesis: Evolutionary forces in the genomic neighborhoods of polymorphic Transposable Elements in plant populations.

(Grade: 10/10)

April-September 2018 MSc. Student (Two-Month Rotations) IMBB/ICS-FORTH Laboratory of “ Microbial pathogenicity and Plant Innate Immunity”in Institute of Molecular Biology-Biotechnology-FORTH (IMBB-FORTH) Group leader: Panagiotis Sarris

Projects involved: Cloning of putative virulence factors (effectors) of the pathogenic bacterium Xylella, into a transient expression system via Agrobacterium.

(Grade: 10/10)

“Population Genetics” Laboratory Institute of Computer Science FORTH. Group leader: Pavlos Pavlidis

Projects involved: Phylogenetic Analysis of Plant Nucleotide Binding Leucine-Rich Repeat (NLR) proteins.

(Grade: 10/10)

“Molecular Plant Biology” Laboratory Department of Biology, University of Crete, Institute of Molecular Biology-Biotechnology-FORTH (IMBB-FORTH). Group leader: Kriton Kalantidis

Projects involved: Screening for CRISPR/Cas9-induced DCL knock-out Nicotiana benthamiana plants

(Grade: 9.5/10)

Undergraduate

Research &

Training

October 2016-

September 2017

Bachelor Thesis University of Patras

Laboratory of “Cytogenetics”, Department of Biology, University of Patras. Group leader: George Mitsainas

Topic: Study of Robertsonian (Rb) Chromosomal diversity of the domestic mouse Mus musculus domesticus (Rodentia: Muridae) in the region of Attica.

(Grade: 10/10)

April 2013-October

2013

March 2013-

September 2013

Undergraduate Intern Sotiria General Hospital

“Clinical Laboratory Diagnosis” Laboratories of Biochemistry, Hematology, Microbiology, Cytology, Anatomical Pathology and Venipuncture at “Sotiria” General Hospital for Chest Diseases

Project: Experienced with all the fundamental technical skills which are basic part of the clinical examinations in biomedical laboratories. Bachelor Thesis University of West Attica

Department of Medical Laboratories, University of West Attica. Group leader: Anna Matzouratou

Topic: Review of Genetic Engineering of T-cells and their use against human diseases. Personal Skills

Practical laboratory

skills and abilities

Molecular Biology techniques

Well qualified in genomic DNA extraction, Polymerase Chain Reaction-PCR, Gel electrophoresis

Molecular cloning and Bacterial transformation

Plant Genetic Modification - Agroinfiltration

ELISA assay

Anatomy and Zoology

Highly skilled in animal, manipulation and anatomy

Animal sample collection (fieldwork)

Cytogenetics

Direct bone marrow and testes chromosomal preparation, Chromosome extraction

Implementation of G- and C- banding chromosome staining techniques

Preparation and study of karyotypes, Microscopy (optical and stereoscopic) Microbiology

Fundamendal microbiology practices (media preparation and autoclaving media and relevant equipment, preparation of streak and spread plates, use of selective media to separate bacteria from mixed culture, cell staining, etc.) Clinical chemistry

All the fundamental technical skills which are basic part of the clinical examinations Programming Python • R • Bash

Image Analysis Adobe Photoshop CS2

Operating Systems Windows • Linux • OS• MS-DOS

Software

packages/Tools and

Applications

Languages

Abilities

MS Office, SPSS, SerialCloner, SnapGene, Picard GATK, Samtools, Bowtie2, BEDOPS, Bedtools2, PLINK, HummerAlign, Belvu, MUSCLE, MAFFT, PAML, MrBayes, JModelTest, PAUP, Dendroscope, RaxmL, LateX

Greek (native)

English Certificate of Proficiency in English (C2) University of Michigan. May 2013. Spanish Diploma de Español como lengua extranjera (Nivel B2) Instituto Cervantes. February 2011.

Excellent communication skills, patient, responsible, able to grasp new concepts quickly and efficiently.

Teaching

experience

Teaching assistant University of Crete

May 2020 Guest Lecturer at Webinar about SARS-CoV-2. Virology Class, Department of Biological Sciences, University of West Attica.

January 2019-March Undergraduate Laboratory Course of “Methods for the functional analysis of biological 2019 macromolecules” (BIO251), Department of Biology November 2018-

December 2018

Undergraduate Laboratory Course of “Fundamental methods of cellular and genetic analysis” (BIO208), Department of Biology.

October 2014-June

2015

Biology courses for high school level Biology at the Community Evening School of Municipality of Patra

Preprints

•Population genomics insights into the recent evolution of SARS-CoV-2. Maria Vasilarou, Nikolaos Alachiotis, Joanna Garefalaki, Apostolos Beloukas, Pavlos Pavlidis doi: https://doi.org/10.1101/2020.04.21.054122

Submitted to Nature Communications (still under review) Poster Presentations

• Evolutionary forces in the genomic neighborhoods of polymorphic Transposable Elements in plant populations. Joanna Garefalaki, Pavlos Pavlidis. 12th Forth Scientific Retreat: FORTH/ICE-HT Conference Center Stadiou St., Platani Patras, Greece, October 14-16 2019

• The traits of contact zones between different chromosomal races of Mus musculus domesticus (Rodentia: Muridae) in two Robertsonian (Rb) systems of Greece. Joanna Garefalaki, Konstantina Chorba, George P. Mitsainas. 6th International Conference of Rodent Biology and Management and 16th Rodens et Spatium : Potsdam, Germany, 3-7 September 2018

Workshops & Seminars

EvoKE 2019 Meeting in Mediterranean Institute for Life Science (MedILS), Split, Croatia

7th International Young Scientists Conference in Computational Science ICS-FORTH Heraklion July 2018

Biosafety Seminar IMBB-FORTH March 2018

1st 2nd and 3rd Greek Medical Laboratory Technologists Conference

12th National Clinical Chemistry Conference University of Patras 2014

Annual Educational Retreat "Sotiria" General Hospital 2013

7th Scientific Retreat of the National Association of Medical Laboratory Technologists: "Quality Control in the Clinical Laboratory" Athens 2013

Other Activities-Volunteering

Volunteer at ANIMA

Science Communication: Participation in Science Communication events (Researchers Night 2017, Patras Science Festival 2017)

Amateur Astronomy (Volunteer at the 10th National Campaign of Amateur Astronomers in Greece)

Drum player in Batala music group

Volunteer as a Biology Teacher for High School level Biology at the Community Evening School of Municipality of Patra (2014-2015)

Blood Donor

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