Joanna Garefalaki
Personal information:
Date of birth: March 1, 1990
Nationality: Greek / American (dual Nationality)
Gender: Female
***************@*****.***
linkedin.com/in/joanna-
garefalaki-02380a5b
Experience
March 2020-present
Research Collaborator
Molecular Microbiology/Immunology Laboratory (Micromol), Department of Biological Sciences, University of West Attica
February 2020-
present
Account and Sales Manager
Medical Gate Ltd.
December 2019-
February 2020
Laboratory Quality Assurance / Quality Control Assistant Alfa Medical S.A.
October 2019-
November 2020
Bioinformatician
Institute of Computer Science-Foundation for Research and Technology Hellas - FORTH
(ICS-FORTH)
Education
2017-2019
MSc. Student in Graduate program of Molecular Biology and Plant Biotechnology
(Current grade: 9.41/10)
Department of Biology, University of Crete and Institutes of Molecular Biology/ Biotechnology and Computer Science - FORTH
2013-2017 Bachelor Degree in Biology
(Grade: 7.04/10)
Department of Biology, University of Patras, Greece 2009-2013 Bachelor Degree in Biomedical Laboratory Sciences
(Grade: 7.2/10)
Department of Medical Laboratories, University of West Attica Graduate Research
& Training
October 2018-
September 2019
MSc. Candidate, Graduate Student Researcher ICS-FORTH Master thesis in “Population Genetics and Bioinformatics” Laboratory (EvoLab) at Institute of Computer Science-FORTH (ICS-FORTH).
Group leader: Pavlos Pavlidis
Master Thesis: Evolutionary forces in the genomic neighborhoods of polymorphic Transposable Elements in plant populations.
(Grade: 10/10)
April-September 2018 MSc. Student (Two-Month Rotations) IMBB/ICS-FORTH Laboratory of “ Microbial pathogenicity and Plant Innate Immunity”in Institute of Molecular Biology-Biotechnology-FORTH (IMBB-FORTH) Group leader: Panagiotis Sarris
Projects involved: Cloning of putative virulence factors (effectors) of the pathogenic bacterium Xylella, into a transient expression system via Agrobacterium.
(Grade: 10/10)
“Population Genetics” Laboratory Institute of Computer Science FORTH. Group leader: Pavlos Pavlidis
Projects involved: Phylogenetic Analysis of Plant Nucleotide Binding Leucine-Rich Repeat (NLR) proteins.
(Grade: 10/10)
“Molecular Plant Biology” Laboratory Department of Biology, University of Crete, Institute of Molecular Biology-Biotechnology-FORTH (IMBB-FORTH). Group leader: Kriton Kalantidis
Projects involved: Screening for CRISPR/Cas9-induced DCL knock-out Nicotiana benthamiana plants
(Grade: 9.5/10)
Undergraduate
Research &
Training
October 2016-
September 2017
Bachelor Thesis University of Patras
Laboratory of “Cytogenetics”, Department of Biology, University of Patras. Group leader: George Mitsainas
Topic: Study of Robertsonian (Rb) Chromosomal diversity of the domestic mouse Mus musculus domesticus (Rodentia: Muridae) in the region of Attica.
(Grade: 10/10)
April 2013-October
2013
March 2013-
September 2013
Undergraduate Intern Sotiria General Hospital
“Clinical Laboratory Diagnosis” Laboratories of Biochemistry, Hematology, Microbiology, Cytology, Anatomical Pathology and Venipuncture at “Sotiria” General Hospital for Chest Diseases
Project: Experienced with all the fundamental technical skills which are basic part of the clinical examinations in biomedical laboratories. Bachelor Thesis University of West Attica
Department of Medical Laboratories, University of West Attica. Group leader: Anna Matzouratou
Topic: Review of Genetic Engineering of T-cells and their use against human diseases. Personal Skills
Practical laboratory
skills and abilities
Molecular Biology techniques
Well qualified in genomic DNA extraction, Polymerase Chain Reaction-PCR, Gel electrophoresis
Molecular cloning and Bacterial transformation
Plant Genetic Modification - Agroinfiltration
ELISA assay
Anatomy and Zoology
Highly skilled in animal, manipulation and anatomy
Animal sample collection (fieldwork)
Cytogenetics
Direct bone marrow and testes chromosomal preparation, Chromosome extraction
Implementation of G- and C- banding chromosome staining techniques
Preparation and study of karyotypes, Microscopy (optical and stereoscopic) Microbiology
Fundamendal microbiology practices (media preparation and autoclaving media and relevant equipment, preparation of streak and spread plates, use of selective media to separate bacteria from mixed culture, cell staining, etc.) Clinical chemistry
All the fundamental technical skills which are basic part of the clinical examinations Programming Python • R • Bash
Image Analysis Adobe Photoshop CS2
Operating Systems Windows • Linux • OS• MS-DOS
Software
packages/Tools and
Applications
Languages
Abilities
MS Office, SPSS, SerialCloner, SnapGene, Picard GATK, Samtools, Bowtie2, BEDOPS, Bedtools2, PLINK, HummerAlign, Belvu, MUSCLE, MAFFT, PAML, MrBayes, JModelTest, PAUP, Dendroscope, RaxmL, LateX
Greek (native)
English Certificate of Proficiency in English (C2) University of Michigan. May 2013. Spanish Diploma de Español como lengua extranjera (Nivel B2) Instituto Cervantes. February 2011.
Excellent communication skills, patient, responsible, able to grasp new concepts quickly and efficiently.
Teaching
experience
Teaching assistant University of Crete
May 2020 Guest Lecturer at Webinar about SARS-CoV-2. Virology Class, Department of Biological Sciences, University of West Attica.
January 2019-March Undergraduate Laboratory Course of “Methods for the functional analysis of biological 2019 macromolecules” (BIO251), Department of Biology November 2018-
December 2018
Undergraduate Laboratory Course of “Fundamental methods of cellular and genetic analysis” (BIO208), Department of Biology.
October 2014-June
2015
Biology courses for high school level Biology at the Community Evening School of Municipality of Patra
Preprints
•Population genomics insights into the recent evolution of SARS-CoV-2. Maria Vasilarou, Nikolaos Alachiotis, Joanna Garefalaki, Apostolos Beloukas, Pavlos Pavlidis doi: https://doi.org/10.1101/2020.04.21.054122
Submitted to Nature Communications (still under review) Poster Presentations
• Evolutionary forces in the genomic neighborhoods of polymorphic Transposable Elements in plant populations. Joanna Garefalaki, Pavlos Pavlidis. 12th Forth Scientific Retreat: FORTH/ICE-HT Conference Center Stadiou St., Platani Patras, Greece, October 14-16 2019
• The traits of contact zones between different chromosomal races of Mus musculus domesticus (Rodentia: Muridae) in two Robertsonian (Rb) systems of Greece. Joanna Garefalaki, Konstantina Chorba, George P. Mitsainas. 6th International Conference of Rodent Biology and Management and 16th Rodens et Spatium : Potsdam, Germany, 3-7 September 2018
Workshops & Seminars
EvoKE 2019 Meeting in Mediterranean Institute for Life Science (MedILS), Split, Croatia
7th International Young Scientists Conference in Computational Science ICS-FORTH Heraklion July 2018
Biosafety Seminar IMBB-FORTH March 2018
1st 2nd and 3rd Greek Medical Laboratory Technologists Conference
12th National Clinical Chemistry Conference University of Patras 2014
Annual Educational Retreat "Sotiria" General Hospital 2013
7th Scientific Retreat of the National Association of Medical Laboratory Technologists: "Quality Control in the Clinical Laboratory" Athens 2013
Other Activities-Volunteering
Volunteer at ANIMA
Science Communication: Participation in Science Communication events (Researchers Night 2017, Patras Science Festival 2017)
Amateur Astronomy (Volunteer at the 10th National Campaign of Amateur Astronomers in Greece)
Drum player in Batala music group
Volunteer as a Biology Teacher for High School level Biology at the Community Evening School of Municipality of Patra (2014-2015)
Blood Donor