Industrial Training Microbiology

Curriculum Vitae

Sumit Kumar Rastogi, PhD

Contact Address C/O A.P Rastogi,

Near Patel club, Vidyapati nagar

Bari road, Baridih

Jamshedpur, Jharkhand

831017, India

Email Address ************@*****.***

Mobile Number 728-***-****/ 997*******

Date of Birth 21

st

January 1987

Nationality Indian

Sex Male

Languages known Hindi & English

Marital status Married

ACADEMICS

Presently working as a “Post-doctoral fellow” in Molecular Microbiology Laboratory, under supervision of Prof. Anindaya Sundar Ghosh, Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India (December 2018- Present)

“Ph.D. in Life Sciences (Microbiology), on the topic entitled “Functional characterisation of a WW domain-containing protein Ifu5 in Candida albicans” under supervision of Dr. Sneh Lata Panwar, Yeast Molecular Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi and Co-supervision of Dr. Nikhat Manzoor, Medical mycology lab, Jamia Millia Islamia University, New Delhi, India (September 2012- July 2019)

“Master of Science” in Biotechnology, University of Lucknow, India, August (2008 - 2010)

“Bachelor of Science” in Biotechnology, C.C.S University, Meerut, India, July (2005- 2008)

Intermediate in Science (Biology), Deoghar College, Deoghar, Jharkhand, India, April (2001- 2003)

High School, Jharkhand School Examination board, Jharkhand, India, March (1991- 2001)

ACHIEVEMENTS

Qualified GATE (Biotechnology), 2011

Qualified C.S.I.R National Eligibility test (NET), 2010

Selected for BCIL-DBT industrial training in biotechnology, 2008

Qualified CEEB (Combined biotechnology entrance examination), 2008 PUBLICATIONS

1. Sumit Kumar Rastogi, Lasse van Wijlick Shivani Ror, Kathy K Lee, Elvira Román,Nikhat Manzoor, Neil A R Gow, Jesús Pla, Joachim F. Ernst and Sneh Lata Panwar (2019). "Ifu5, a WW domain-containing protein interacts with Efg1 to achieve coordination of normoxic and hypoxic functions to influence pathogenicity traits in Candida albicans, Cellular Microbiology. DOI: 10.1111/cmi.13140 2. Sumit Kumar Rastogi, Yamini Sharma and Nikhat Manzoor (2019). β-citronellol alters cell surface properties of Candida albicans to influence pathogenicity related traits. Medical Mycology. DOI: 10.1093/mmy/myz009

3. Shilpa Pal, Jyoti Verma, Sathi Mallick, Sumit Kumar Rastogi, Akash Kumar and Anindya S. Ghosh (2019), Absence of the glycosyltransferase WcaJ in Klebsiella pneumoniae ATCC13883 affects biofilm formation, increases polymyxin resistance and reduces murine macrophage activation. Microbiology. DOI 10.1099/mic.0.000827

4. Sonali Mishra, Sumit Kumar Rastogi, Sangeeta Singh, Sneh Lata Panwar, Manoj Kumar, Kumar Shrivashde, Krishna Mishra (2019) “Controlling pathogenesis in Candida albicans by targeting Efg1 and glyoxylate pathway through naturally occuring polyphenols”, Molecular Biology Reports. DOI:10.1007/s11033-019- 05014-z

5. Yamini Sharma, Sumit Kumar Rastogi, Saiema Ahmedi & Nikhat Manzoor

(2020). Antifungal activity of β -citronellol against two non-albicans Candida species. Journal of Essential Oil Research. DOI: 10.1080/10412905.2020.1737588 6. Srivastava A, Sircaik S, Husain F, Thomas E, Ror S, Rastogi S, Alim D, Bapat P, Andes DR, Nobile CJ, Panwar SL. (2017). Distinct roles of the 7-transmembrane receptor protein "Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans. Cellular Microbiology 19(12). DOI: 10.1111/cmi.12767 7. Yamini Sharma, Sumit Kumar Rastogi and Nikhat Manzoor (2018). Anti-Candida activity of geraniol: effect on hydrolytic enzyme secretion and biofilm formation. Journal of pure and applied microbiology 12 (3)

SELECTED SKILLS AND TECHNIQUES

Molecular biology techniques:

DNA and RNA isolation, PCR, Cloning, Transformation methods for bacteria and yeast, Southern hybridization, Quntitative Real-Time PCR (qPCR)

Protein biochemistry techniques:

Protein isolation and purification (AKTAprime plus), SDS-PAGE and Western blotting, Functional characterization of novel protein, GFP, HA and Myc tagging of proteins, immunofluorescence, Co-immunoprecipitation assay, Yeast two-hybrid assay

Microbial techniques:

Different staining methods, Culture techniques of bacteria and yeast, Spot assays, Minimum inhibitory concentration (MIC), Virulence assay, Drug efflux and influx assay, Growth curve, Flocculation assay, Adhesion assay, Biofilm assay

Microscopy:

TEM and SEM analysis, Light microscopy, fluorescence microscopy, Confocal microscopy

Bioinformatics:

NCBI, Genetic databases and homology search (BLAST, Clustal W), Protein structure prediction and molecular docking (Swiss modelling, PyMol, PyRx, DoGsite scorer, RAMPAGE), Microarray data analysis

TRAINING, CONFERENCES AND WORKSHOPS

Presented poster on science day, 28

th

feb 2018, JNU, New Delhi

Presented poster in CYB (conference on yeast biology), January 2018, JNU, New Delhi

Attended the Workshop on “Enhancing Research Impact” hosted by Oxford University Press and JNU, 2016, New Delhi, India

Presented poster on science day 2016, JNU, New Delhi, India

Presented poster in ICYB (International Conference on Yeast Biology)2013, IMTECH, Chandigarh, India

Participated in ICYB (International Conference on Yeast Biology) 2011, IIT Mumbai, India

Certificate course on Radiation safety, Jawaharlal Nehru University, New Delhi, India

Attended workshop on FACS by BD biosciences, New Delhi, India

Certificate course on “Microarray data analysis”, In silico biology, Lucknow, India DECLARATION

I hereby declare that all the information stated above is correct and true to the best of my knowledge.

(Sumit Kumar Rastogi)

REFERENCES

Dr. Nikhat Manzoor Prof. Anindya Sundar Ghosh

Associate professor Professor

Department of Biosciences Department of Biotechnology Jamia Millia Islamia University Indian Institute of Technology New Delhi, India Kharagpur, West Bengal, India

Email: ******.*******@*****.**.** Email- *******@**.******.**.**

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