Customer Service Quality

Qualification Summary

Daily working experience with volumetric glassware (volumetric flasks, pipettes, burettes, graduated cylinders, etc.), quantitative transfer, solution preparation, and serial dilutions

Perform and analyze experiments to support the advancement of drug discovery programs

Follow protocols to execute both small-scale and at-scale experiments

Assist other staff to complete all laboratory tasks as necessary

Experience performing quality control testing per various pharmacopeia, including USP, NF, FCC, and EP monographs

2 years background performing and documenting testing GDP/GLP setting

Proven track record of error-free work

Proficient in laboratory analytical techniques of : Determination of Potency, Related Compound and Organic Impurity Analysis via HPLC/UPLC and GC, Gel-Filtration, Ion-Exchange, and Affinity chromatography systems, Thin Layer Chromatography, SDS-Page, Titrimetry (including potentiometric analysis), Spectroscopy, Distillation, as well as familiarity with Proton and Carbon NMR techniques

Experience with basic molecular biology techniques: PCR, DNA cloning and sequence analysis

Chromatography Data System: Waters Empower 3 proficient in creation of instrument methods, sample set methods and report methods

Bacterial cell culture, fermentation, protein purification and bio separation techniques during biochemistry coursework Molecular biology Techniques: Western Blotting, ELISA, PCR, Polyacrylamide Gel Electrophoresis, Isolation, Extraction and Purification of DNA, RNA and proteins, Recombinant DNA technology, Media preparation

Bio-separation and purification techniques: Microfiltration, Centrifuge, Qiagen gel extraction kit

Education

University of Illinois in Chicago, IL Dec 2017

Bachelor of Science – Biochemistry

Skills

Fluent in Polish

Proficient in the use of Chemdraw, Origin, PyMol, and Microsoft Office applications, especially in the use of Excel for data analysis

A++ Computer Repair Certified with outstanding computer skills

Excellent written and oral communication skills

Comfortable working independently as well as leading and managing small groups

Problem solving mentality driven to find solutions and improve current practices

Comfortable learning and adapting to new software programs, equipment and protocols

Experience and previous commendations in customer service and client interactions

Professional Experience

First Priority, Inc. Elgin, IL Feb 2018 – Aug 2019

Quality Control Finished Product Group, Chemist I - Veterinary Pharmaceutical Production Facility

Daily working experience in the use, maintenance, calibration, and repair of laboratory equipment, including but not limited to:

Chromatography Systems:

UPLC: Waters Acquity

HPLC: Agilent Infinity, Infinity II and Agilent 1200, Waters Alliance 2690

Analytical Equipment: Spectrophotometers (UV/Vis, FT-IR), Karl Fischer Titrator, Melting Point Apparatus, Densitometer, Moisture Balance, Analytical Balance, pH Meter, Conductivity Meter, Kinematic Viscometers, Muffle Furnace, Vacuum Oven, Microscope

Performed verification analysis on in-process and finished products of veterinary pharmaceuticals

Created test methods and controlled documents in order to increase testing efficiency and improve consistency of testing procedures while minimizing errors

Documented testing procedures and results in paper notebooks/controlled documents per GDP

Responsible for the review of testing completed and documentation prepared by other analysts

Performed vendor qualification testing on representative samples from prospective new raw material manufacturers to approve the purchase and use of materials in finished product formulation

Have worked in Biosafety Cabinets regularly while testing process

Managed finished product testing program, including scheduling tasks for multiple group members

Managed and organized hazardous lab waste disposal safely using all appropriate PPE

Assisted in the investigation of non-conformances, determination of root cause and subsequent corrective/preventive actions; responsible for closing investigations initiated within the work group

Trained quality control personnel on equipment usage and testing procedures

Prepared and submitted protocol documentation for FDA approval.

Updated existing test methods and Standard Operating Procedures to comply with cGMP/GLP practices

Administrator for Empower 3 Chromatography Data System, responsible for method development

University of Illinois in Chicago, Chicago, IL Jan 2017 – May 2017

Laboratory Research

Tested whether the mutation S68C of wild-type TEM-1 β-lactamase enzyme impacts enzyme kinetics

Introduced forward and reverse mutant primers to the wild-type enzyme

DNA amplified using PCR reaction and introduced to the mutant primers into the wild-type DNA

Inserted mutated DNA into plasmid vectors in order to produce desired antibiotic resistant E. Coli cell culture

Crude mutated DNA extracted via PCR and gel electrophoresis, followed by the addition of digestion enzymes EcoRI and HindIII to create sticky ends in both the mutated DNA and bacterial vector

Transformation use to insert ligated mutant DNA into E.coli cells to begin vector cloning

Used various extraction techniques such as dialysis, gel-filtration, and ion-exchange to purify crude protein

Performed SDS-page, BCA assay, and activity assay to determine that the S68C mutation decreased the activity significantly, thus determining that the active site of TEM-1 β-lactamase is located at position 68

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