Molecular Phd
Resume:
K. VINOTHKUMAR, PhD
Institute of Life Sciences, Bhubaneshwar, Odisha, India 751023.
Mobile: +91-963*******; E. mail: *************@*****.*** PROFILE
Research experience in the fields of Molecular Microbiology and Molecular Oncology
Experienced in molecular biology techniques (qPCR, gene cloning and protein expression)
Doctorate in the field of bacterial antibiotic resistance with good publication records
Developed PCR-based species specific molecular identification assay for three Vibrio spp.
Skilled in handling microbial culture, mammalian cell culture and laboratory animals
Team player with good communication and writing skills EDUCATION
2016
Doctoral Degree (PhD)
Biochemistry
The M. S. University of Baroda,
Vadodara, India.
2009
Master’s Degree (M.Sc.)
Biotechnology
Bharathidasan University,
Tiruchirappalli, India.
2007
Bachelor’s Degree (B.Sc.)
Zoology with Biotechnology
A.V.V.M. Sri Pushpam College,
Poondi, Thanjavur –Dt, India.
RESEARCH EXPERIENCE
PhD thesis: Molecular characterization of quinolone resistance in the clinical isolates of Vibrio and Shigella spp.
Duration Institution Particulars of work done
August 2017 to April
2020
Institute of Life
Sciences,
Bhubaneshwar, India
Worked as a Post-doctoral fellow on a project
involved in understanding the role of specific genes in disease progression of Chronic myeloid leukemia. June 2014 to June
2017
Indian Institute of
Advanced Research,
Gandhinagar, India.
Worked as a Senior research fellow on the project
entitled “Function characterization of molecular
factors that confer quinolone resistance in Vibrio spp.”
March 2010 to May
2014
Indian Institute of
Advanced Research,
Gandhinagar, India.
Worked as Junior research fellow on different
projects involved in studying the antibiotic resistance in Vibrio and Shigella spp., determining toxic
potential of environmental V. cholerae isolates and development of PCR based rapid identification
technique for three major Vibrio spp.
K. Vinothkumar, PhD 2
TECHNIQUES KNOWN
Molecular Biology-
Nucleic acid isolation (genomic DNA, plasmids, RNA), DNA cloning, Bacterial transformation
Recombinant protein expression, PAGE, Western blot
Multiplex PCR, RT-PCR, qPCR
SNP analysis, PFGE (Pulsed Field Gel Electrophoresis), RAPD, ChIP
Gene knock down using PNA, SiRNA and ShRNA
Microbiology-
Experienced in working on BSL-2 with pathogens such as Vibrio spp. and Shigella spp.
Biochemical tests for bacterial identification, Resistance phenotyping, Minimum inhibitory concentration assay, Mutant prevention concentration assay, Drug accumulation assay
Hemolytic and Haemagglutinin Assay, Protease assay, Cytotoxic assay Computational Biology
Bioinformatics- Basic tools for nucleotide and protein sequence analysis Cell Biology
Mammalian cell culture techniques, MTT assay, Transfection, Transduction, FACS
Experienced in working with laboratory mice (Lineage negative cells isolation from bone marrow, Transplantation of bone marrow cells, Injection: Intravenous, Intraperitoneal, Subcutaneous) PUBLICATIONS
Research Papers: 5 Review article: 1 Book Chapters: 3 RESEARCH TRAINING IMPARTED
Trained two research fellows for the research projects in the laboratory at IIAR, Gandhinagar.
Conceived and designed the dissertation works of one postgraduate student and six undergraduate students and trained them in the laboratory at IIAR, Gandhinagar. AWARDS/ACHIEVEMENTS
1. Awarded National Post-Doctoral Fellowship from Science and Engineering Research Board, Department of Science and Technology and worked on the project entitled “Decoding the role of proto-oncogene Ecotropic Viral Integration Site I (EVI1) in Chronic Myeloid Leukemia-Blast Crisis
(CML-BC)” from April 2018 to April 2020 in ILS, Bhubaneshwar. 2. Awarded Summer Research Fellowship jointly sponsored by Indian Academy of Science
(Bangalore), Indian National Science Academy (New Delhi), and National Academy of Science, India (Allahabad) during May to July 2008 and worked as a summer trainee in IIAR, Gandhinagar. K. Vinothkumar, PhD 3
PUBLICATION LIST
Research Papers:
1. Vinothkumar K, Bhalara SR, Shah A, Ramamurthy T, Niyogi SK, Kumar GN and Bhardwaj AK. Involvement of topoisomerase mutations and qnr and aac (6’) Ib-cr genes in conferring quinolone resistance to the clinical isolates of Vibrio and Shigella spp. from Kolkata, India (1998-2009). J Glob Antimicrob Resist. 2018; 13: 85-90.
2. Vinothkumar K, Kumar GN and Bhardwaj AK. Characterization of Vibrio fluvialis qnrVC5 gene in native and heterologous hosts: Synergy of qnrVC5 with other determinants in conferring quinolone resistance. Front Microbiol. 2016; 7:146. 3. Rajpara N*, Vinothkumar K*, Mohanty P, Singh AK, Singh R, Sinha R, Nag D, Koley H, Bhardwaj AK. Synergistic effect of various virulence factors leading to high toxicity of environmental V. cholerae non-O1/ non-O139 isolates lacking ctx gene: Comparative study with clinical strains. PLoS One. 2013; 8(9): e76200. (* contributed equally) 4. Vinothkumar K, Bhardwaj AK, Ramamurthy T, Niyogi SK. Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis. Diagn Microbiol Infect Dis. 2013; 76(4):526-8. 5. Singh R, Rajpara N, Tak J, Patel A, Mohanty P, Vinothkumar K, Chowdhury G, Ramamurthy T, Ghosh A and Bhardwaj AK. Clinical isolates of Vibrio fluvialis from Kolkata, India, obtained during 2006: Plasmids, qnr and mutation in gyrase A as mechanisms of multidrug resistance. J Med Microbiol. 2012; 61: 369-374.
Review article:
1. Bhardwaj AK, Vinothkumar K, Rajpara N. Bacterial quorum sensing inhibitors: attractive alternatives for control of infectious pathogens showing multiple drug resistance. Recent Pat Antiinfect Drug Discov. 2013; 8(1): 68-83.
Book Chapters:
1. Vinothkumar K, Rajpara N, Nair M and Bhardwaj AK (2017) Multidrug resistance in Vibrio spp. and Shigella spp.: Emergence of untreatable pathogens. Resistance to Antibiotics: Are we prepared to handle this growing ghost? ed. Bhardwaj AK (Foster City: Omics group ebooks), 9-22. 2. Bhardwaj AK and Vinothkumar K. (2015) Evolution of MDRs. Quorum Sensing Vs Quorum Quenching: A battle with no end in sight, ed.V. C. Kalia (New Delhi: Springer), 9-22. 3. Bhardwaj AK, Vinothkumar K, Rajpara N, Mohanty P and Kutar BMRNS (2014) Therapeutic Limitations due to Antibiotic Drug Resistance: Road to Alternate Therapies. Frontiers in Anti- Infective Drug Discovery, Vol.3, eds. A. Rahman and M. I. Choudhary (Sharjah: Bentham Science Publishers), 72-141.
PERSONAL DETAILS
Permanent Address: 2-69, South Street, Sigar and post, Nagapattinam- Dist, Tamil Nadu, Pin: 610106 Languages Known : Tamil, English, Hindi
Date of Birth : 18th August 1987
Marital Status : Married
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