Laboratory Technician

Katie Valencia

********.********@*****.*** 720-***-**** Brighton, CO www.linkedin.com/in/kaitlynnv

Qualifications

Four plus years spent as a critical piece to research specializing in biochemistry, microbial ecology, genetics, and molecular and cellular biology

Molecular biology techniques such as preparing various environmental samples for Next Generation Sequencing, PCR, ELISA, cell staining, western blotting, and bacterial cloning

Over three months’ handling and studying the model species C. elegans

Frequently presents results in both written and oral format to lab mates and peers

Education

Bachelor of Science in Biochemistry, Minor in Chinese Graduated: May 2020

Colorado State University, Fort Collins, CO GPA: 3.3

Graduated from an American Society for Biochemistry and Molecular Biology program

Studied abroad Summer 2019 in Shanghai, China

Research Experience

Laboratory Technician August 2019 – Present Microbial Ecology, Colorado State University

Worked individually and in a team on over six large scale projects to upgrade workflow in library preparation procedures for Next-Generation Sequencing

Tested DNA extraction and PCR protocols to troubleshoot and rewrite multistep SOPs efficiently obtaining pure DNA for downstream sequencing applications

Ran over 100 Qubit assays and Agilent TapeStation gels to compile and calculate raw data for more accurate quality assurance

Prepared over 1,000 final library samples in two months for Next-Generation Sequencing via DNA extraction, ligation, indexing PCR, and DNA fragment clean up and size selection

Agronomy Foundation Seed Assistant May 2018 – August 2018

Agricultural Research, Colorado State University

Maintained genetic purity of more than 12 different varieties of wheat crop by identifying and knocking out off-types and mutants in the population

Assisted in the process of seed production and packaging of over 2,000 pounds of wheat seed a week by following specific GMP instructions and protocols

Sterilized large work equipment for seed production under a zero-tolerance seed policy to prevent contamination

Undergraduate Research Laboratory Assistant

Bioagricultural Sciences, Colorado State University August 2017 – May 2018

Determined quantity of seeds, dimensions of flowers, and kept clear records for more than 2,000 Canadian Thistle collected from fieldwork

Utilized leafJ analysis to collect and enter data on surface area and width of Canadian thistle leaves to assess environmental impact on development

Germinated and nursed over 1,000 Canadian Thistle plants from seeds in petri dishes

MCD Biology, University of Colorado Boulder May 2017 – August 2017

Produced, maintained, and stained over 15 different strains of worm populations by conducting gene knockouts to manipulate muscle cells and fat storage

Routinely synchronized worm populations 2-3 times a week involving an 8-hour procedure

Identified and imaged changes in fat storage and development of over 100 C. elegans weekly under fluorescent and DIC microscopes

Over 100 hours spent using ImageJ analysis to quantify, compile, and analyze data on intensity and diameter of fat cells which was delivered in a presentation to lab team

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