Mammalian cell culture, qPCR

PRIYANKA VAISHAMPAYAN

************.*@************.*** 857-***-****

Education:

Northeastern University September 2018- August 2020 Master of Science in Biotechnology GPA: 3.83/4

Mumbai University August 2015- January 2018

Master of Pharmacy GPA: 7.5/10

Thesis Title: Modified release drug delivery system: Formulation and in vitro evaluation Mumbai University September 2011- May 2015

Bachelor of Pharmacy GPA: 7.0/10.0

Research Experience:

LogicBio Therapeutics, Lexington.

Co-Op Process Development January2020-June2020

Passaged and maintained mammalian HEK 293T cells

Carried out various experiments to support optimization of triple transfection method for AAV viral vector production

Monitored cell density and cell culture metabolites of samples culture post-transfection

Measured AAV vector titers by qPCR method to compare volumetric productivities

Operated small-scale benchtop bioreactors for optimization of upstream process parameters

Purified viral vectors by affinity chromatography with AKTA system

Familiar with Depth Filtration (DF) and Tangential Flow Filtration (TFF) Center for Drug Discovery, Northeastern University, Boston. Graduate Research Assistant September 2018- December 2019

Involved in a project, biophysical and biochemical studies on human monoacylglycerol lipase

(hMGL) and fatty acid binding protein-7(FABP-7), regulating endocannabinoid proteins

Expressed hMGL and FABP enzymes and mutants in E. coli cells

Purified proteins using affinity chromatography resin

Carried out biochemical assays on hMGL and FABP-7 using LC-MS

Performed NMR experiments to analyze conformational changes as well as ligand binding on hMGL enzyme

Supervised and trained two highschool students from ACS Project SEED Program Gansons Ltd, Thane, India.

Graduate Intern: Master of Pharmacy August 2016- January 2017

Formulated coated pellets with drug and polymer utilizing Ganson’s fluidized bed processor coater

Optimized pellet coating process parameters and formulation parameters

Compared drying efficiency using different plates in fluidized bed drier

Skills:

Laboratory skills: Mammalian & Bacterial cell culture, SDS PAGE, Protein purification, Biochemical assays, UV-vis spectrometry, TLC, HPLC, Nanodrop, Autoclave, Dissolution apparatus, Brookfield’s viscometer, Benchtop Bioreactor, AKTA, qPCR, Transfection, Transformation Computer skills: Pymol, Design expert, JMP

Contact this candidate