ANUSHA RENGARAJAN

Email: ade7qw@r.postjobfree.com

PERSONAL

Date of Birth: 16th July 1997

Citizenship: Indian

CAREER OBJECTIVE

Seeking a position as a PhD in your esteemed lab which provides an apt platform for exhibiting my passion for research in developmental, molecular biology. EDUCATION

Completed B-Tech in Biotechnology from SRM Institute of Science and Technology, with 85.18 percentile, 2015-2019

Completed schooling from The School K.F.I, ISCE board, 2015. RESEARCH EXPERIENCE

B-Tech final year thesis: I worked on the project studying “Signature of linker histone protein, H1T2, during rat spermatogenesis” for a period of 6 months from December 2018 to June 2019, under Prof M.R.S.Rao, chromatin biology laboratory, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Bangalore

Project: Creating mutant constructs of small RNA HPnc2630 in Helicobacter Pylori strain SS1 using splicing by overlap extension PCR for a period of two months, February to April 2018, under Dr. Oindrilla Mukherjee, in SRM Institute of Science and Technology, Chennai Work Experience: Understanding the role of Eomesoderm paralogues in Zebrafish Cerebellum development from September 2019, under Dr. Sreelaja Nair, Tata Institute of Fundamental Research (TIFR), Mumbai.

SHORT-TERM INTERNSHIPS

1. Worked in Center for Cellular and Molecular Biology, Hyderbad, for 1 month under Dr. Rakesh Mishra (2018).

2. Completed a month’s course on animal cell culturing in MIT AU_KBC Lab Chennai Chrompet under Dr. Vimalraj(2017).

3. Studied Flow cytometry and its application in diagnosing leukemia in Apollo Cancer Hospital Chennai(2017)

4. Is familiar with liquid chromatography techniques of Agilent Technologies(2016) 5. Completed a project on Genome Analysis at Agilent Technologies, Bangalore, under Dr. Deepak(2016)

6. Completed project on Hysteresis in University of Madras Chennai(2015) 7. Completed Project on enzyme in Sashun Chemical Chennai(2014) RESEARCH INTERESTS/PROJECTS

Chromatin Biology, Developmental Biology in particular embryonic development, Transcriptomics, Cell Biology, Genomic Biology, Epigenetics in particular epigenetic inheritance.

COURSES UNDERTAKEN

Basic Biological Chemistry, Stem Cells and Development, Bioinformatics, Intracellular traffic, Immunology, Animal Biology, Cancer Biology

THESIS SUMMARY

During rat spermatogenesis the chromatin undergoes dramatic remodeling; the chromatin changes from a round active state to an elongated inactive state, testis-specific linker histone H1T2 is said to play a role in the process of remodeling. It has been shown the normal chromosome structure are replaced with more basic proteins during this process. H1T2 shows a specialized localization at the “apical pole”. This is considered to be an epigenetically important area, and is hypothesized to play a role in evicting histones from the mature sperm. To understand the global function of H1T2, I studied the repertoire of the interacting protein partners of H1T2, as well as it’s genome wide occupancy. The significance of the project was to characterize H1T2, which could potentially explain the dramatic changes that occur to the nucleosome during spermatogenesis. My observation was in accordance to the existing studies which show that a different nucleosome set up is seen in the elongating rat spermatids when H1T2 is expressed. The methods used for understanding the possible role played by the linker histone protein H1T2 were, 1) Antibody validation, 2) chromatin immuno-precipitation studies 3) mass-spectrometry studies.

CONFERENCE AND WORKSHOPS

1. International Conference of Immunology,

2. Presented a poster at this conference on “The role of antiangiogenetic markers and molecules in immunosuppression of tumor cells”, which was held in SRM Institute of Science and Technology, from September 26-28, 2018. 3. Attended a National level Workshop on Real time PCR techniques in SRM University KTR, 2017

4. Attended Math workshop in CMI (20114,2015)

5. Won best physics project in SRM University Tech fest, KTR (2015) 6. Participated in the Science Outreach to School at M.S.SWAMINATHAN RESEARCH FOUNDATION (MSSRF) (May-2014).

RESEARCH SKILLS

• Experimental Skills:

Gene Cloning, Site Directed Mutagenesis, Protein Expression and Purification (His/GST tagged), Western Blotting, Protein-protein Crosslinking, DNA-protein Cross-linking, Chromatin Immunoprecipitation, Immunofluorescence on fixed samples, Epifluorescence Microscopy, Histone Extraction from Tissues/Cell Lines, Immunoprecipitation, Ultracentrifugation, In vitro kinase assays, Mass Spectrometry, Dot Blotting, Pep- tide Competition Assay, Animal Cell Culture, Animal (Rat, and Zebrafish) Handling, Plasmid Isolation, Double Insitu-hybridization, Cryosection of zebrafish brain samples using vibrotome and cryostat, Invitro-Fertilization of Zebrafish embryos, Microinjections on zebrafish embryos.

• Computational Skills: ‘Rstudio’ Programming

EXTRA CURRICULAR ACTIVITIES

1. Part of the Basketball team in The School K.F.I (2012-2015) 2. Learning Classical Music(from 2001)

3. Learning Classical Instrument Veena(2010) )

4. Worked in a Non-Government Organization in Kancheepuram called Hand-in- Hand(2013)

5. Participated in a theatre production for the play Ponniyin Selvan,Magic Lantern(2014) 6. Worked in Aurovile(Pondichery) to get a hands on experience in organic farming

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