Poorvaja Ganesan

ade5xa@r.postjobfree.com 240-***-**** https://www.linkedin.com/in/poorvaja-ganesan-04822510b/ College Park, MD EDUCATION

M.Engg Project Management (Minor: Bioengineering), University of Maryland, College Park Spring 2018 – Spring 2020

● Courses: Biomaterial Applications, Medical Device Development, Drug Development, Bioinformatics, SCRUM, Agile Development, Product Management & 3D- Printing Techniques B.Tech Biotechnology, Sri Venkateswara College of Engg (Anna University) 2013 – 2017 TECHNICAL SKILLS

● Techniques: ELISA, Western Blotting, Protein Quantitation, Sensitivity Assay, Microscopy, Cell Staining, Spectrophotometry, Animal Handling, Immunodiffusion, Transformation, Plasmid Isolation

● Databases: GeneBank, EMBL, SWISS-PROT, NCBI, Pfam, PDB, KEGG, ExPasy, OMIM, NDB, Entrez

● Tools: BLAST, FASTA, Nanoscribe GT Photonic, MATLAB, Snapgene, PCR, Microarray, EMBOSS, Cytoscape, Python, R WORK EXPERIENCE

Research Assistant & Manufacturing Management Intern, Gel-e Lifesciences Inc. (Maryland) Jun 2019 – Present

● Toxicology Studies: Performed Biocompatibility testing, Cytotoxicity testing using agar diffusion (direct contact), Acute systemic toxicity and Irritation (intracutaneous injection) testing using extraction methods

● Characterization Testing: Tested and optimized the antibacterial surgical hemostat for Temperature, Performance measurement, Viscosity, pH and turbidity.

● Product development management, Double-barrel syringe study, FDA documentation, prepare and test samples of modified biopolymer, organize and perform animal studies, optimization of protocol and preclinical studies relating to antibacterial surgical hemostat and in-vitro and in-vivo characterization for the same. Research Assistant, Gene Editing & Genomics Lab (UMD) May 2018 – Sept 2019

● Developed an instructional CRISPR-Cas9 laboratory course for lower-level undergraduate bioengineering program and skilled in mini-preps, transformation, bacterial cell culture, preparation and isolation of plasmids and competent cells.

● Tested and optimized the CRISPR Cas9 ODIN kit (E. coli). Designed experiments for PCR amplification and analysis of muted cell lines and performed Western blotting. Created new CRISPR Cas9 kits (yeast) for future experiments. Bioinformatics Intern, BioZone Research Technologies Ltd. (India) Jan 2016 – Feb 2016

● Analyzed and retrieved genomic data and network analysis using Cytoscape, BLAST, FASTA, Ramachandran Plot and Autodock for accurate identification of defects in the human genome Research Intern, IIT Madras (India) May 2015 – Jul 2015

● Synthesized Hydroxyapatite compound through chemical deposition and wet-chemical methods and characterized by X- Ray diffraction method, SAED, and thin-layer Chromatography techniques. Research Intern, Frontier Mediville Lifeline, (India) Dec 2014 – Jan 2015

● Performed DNA/RNA isolation, PCR, Agarose gel electrophoresis, SDS PAGE, Silver staining, Primer designing, sterilization of cell culture, cell passaging & revival of cell lines ACADEMIC PROJECTS

Medical Device Development for detecting Oxygen Concentration using CPAP Therapy Aug 2018 – Dec 2018

● Developed a proposal and submitted a detailed FDA report in accordance with FDA regulations (including FMEA Analysis) for a pediatric oxygen concentrator with real-time monitoring using CPAP therapy to treat children who have difficulties in breathing.

Novel Gene Discovery Aug 2018 – Dec 2018

● Novel gene discovery and characterization correlating Guanine nucleotide-binding protein G(k) subunit alpha in Homo sapiens to a homologous gene in a vascular plant organism, Pinus taeda using Matlab to obtain all features and descriptions including genes, pseudogenes, and proteins.

Developed Hybrid Biomaterial for Wound Healing and Angiogenesis Jan 2018 – May 2018

● Developed a proposal for a sturdy chitosan hydrogel with growth factor encapsulated nanoparticles to promote better healing and angiogenesis in wounds

● Compared to existing techniques this method involves minimal invasion into the human body with targeted healing of deep wounds through sophisticated biomarkers in the form of nanoparticles Identification of novel stage-specific Biomarkers in Lung and Cervical Cancer Aug 2016 – May 2017

● Proposed a novel method for the identification of biomarkers in specific cancer types focused on stage specific Biomarkersthat leads to earlier detection of cancer in patient

Callus Development of Phyllanthus amarus& Coriander sativum& Phytochemical Analysis Feb 2015 – May 2015