Clinical Research Assistant
Resume:
KRISTEN SPITLER
***** ********** **. ****** ******* FL, 33617
Phone: 813-***-**** Email: *********@*****.*** July 30, 2020
To Whom it May Concern,
For the last year and a half I have been working under Dr. Marco Davila at Moffitt Cancer Center, in collaboration with a reputable pharmaceutical leader in T cell therapies to develop and deliver various CAR T products to the FDA for approval. Starting with protocol design and model optimization, I have managed every aspect of our pre-clinical in-vivo work and assisted with the in-vitro selection process. I have gained expertise in retroviral transfections and transductions, flow cytometry, protein quantification and T cell functional assays, as well as analyses of the aforementioned. In this role I have enhanced my teaching and mentorship skills, training peers and graduate students technical skills and processes for successful in-vivo experimental designs. Furthermore, I have become an avid oral and written communicator, generating figures and reports for presentation at monthly joint research meetings, held with our industry partners. I previously worked as a Research Associate for the University of South Florida for three and a half years. In this role I was partnered with Moffitt Cancer Center to implement a variety of in-vivo projects. I worked closely with Dr. Christine Chung’s lab to explore EGFR inhibitors and immune cell tracking in the tumor microenvironment with regards to head and neck squamous cell carcinoma. My immediate supervisors commended me for my technical skills, organizational abilities, and frequently asked to step in to mitigate difficult situations. While working for the University of South Florida I obtained my Laboratory Animal Technologist (LATG) certification with the American Association of Laboratory Animal Sciences (AALAS). Though I enjoy my current role of uncovering breakthrough science in academia, I believe I am better suited for a role in the industry setting. I am confident that my ability to pursue scientific discovery, standardize and optimize research processes and adhere to strict deadlines makes me to the perfect candidate for this Research Associate Position. I am a fast learner who excels at multi-tasking, detailed documentation, time management, data acquisition, analyses and collaboration. Moreover, I am innovative, enjoy learning new techniques, and thrive in environments where I am able to work independently, but collaboratively. Thank you,
Kristen Spitler
KRISTEN SPITLER
11308 Grandville Dr.
Temple Terrace FL, 33617
*********@*****.***
SUMMARY
Industrious Research Technician with background in studying CAR T immunotherapies. Detail-oriented and adept at managing deadlines and developing systematic approaches to tasks.
SKILLS
• Pre-clinical research modeling
• Detailed documentation
• Project & Time Management
• Leadership & Mentorship
• Flow Cytometry
• Scientific Writing & Presentation
• In-vivo, Ex-vivo, In-vitro
• Tissue Culture
EXPERIENCE
Research Assistant II
Moffitt Cancer Center/Jan 2019 to Current
Tampa, Florida
• Assisted in study planning, research and development of new CAR T candidates in collaboration with Atara Pharmaceuticals.
• Processed clinical patient samples and maintained organized records, in partnership with Precigen.
• Performed retrovirus production, culturing and transduction of primary and murine T cells.
• Applied multi-color Flow cytometry panels for phenotype and function analysis.
• Prepared tables, graphs, fact sheets and written reports summarizing research results.
Research Associate
University of South Florida/Jun 2015 to Jan 2019
Tampa, Florida
• Proficient in a variety of mouse handling skills, including dosing, tissue collections, project management, and colony upkeep.
• Delivered comprehensive training to new technicians on equipment, technical skills, inventory maintenance, and GLP standards.
• Radiation safety trained for working with isotopes and (Cs-137) irradiator use.
• Heavily relied on by supervisors for ability to accommodate for changes and problem solve in complicated or potentially sensitive situations. Highlights:
• Collaborated with Dr. Christine Chung’s lab on monitoring PD-1, EGFR, and CHK1 inhibitors against head and neck squamous cell carcinoma tumor models.
• Collaborated with Dr. Pearlie Burnette’s lab on monitoring the effects of knocked out Cereblon on T cell and hematopoietic function.
• Collaborated with Dr. Robert Gillies lab on studies analyzing Doxorubicin in combination with L-DOS47.
EDUCATION
Bachelor of Applied Science: Public Health
University of South Florida
Tampa, Florida
Due for Graduation in December 2020
Associate of Applied Science/Arts: Business Administration Pasco-Hernando State College
New Port Richey, Florida
Graduated: Apr 2013
COMMUNITY OUTREACH EXPERIENCES
City of Temple Terrace Appointed River Watch Committee Member, Mar 2020 Hillsborough County Guardian Ad Litem Child Advocate Volunteer, Jan 2020 Pasco County Mounted Posse Volunteer, Jun 2015-Jun 2016 SELECTED PUBLICATIONS
Boucher, J.C., Li, G., Kotani, K., Cabral, M.L., Morrissey, D., Lee, S.B., Spitler, K., Beatty, N., Shresthra, B., Yu, B., Aslamuzzaman, K., Weng, X., Sebti, S., Davila, M.L.
(2020). Mutation of the CD28 Costimulatory Domain Confers Enhanced CAR T Cell Function. Blood. 134(Supplement_1):248. https://doi.org/10.1182/blood-201*-****** Full paper provisionally accepted to Cancer Immunology Research Faramand, R., Jain, M., Staedtke, Verena, Kotani, H., Bai, R., Reid, K., Lee, S.B., Spitler, K., Wang, X., Cao, B., Pinilla, J., Lazaryan, A., Khimani, F., Shah, B., Chavez, J., Nishihori, T., Mullinax, J., Gonzalez, R., Hussaini, M., Dam, M., Brandjes, B., Bachmeier, C., Anasetti, C., Locke, F., Davila, M.L. (2020) The Tumor Microenvironment and Inflammation Influence Toxicity in Patients with Large B Cell Lymphoma Treated with Axicabtagene Ciloleucel.
Paper provisionally accepted to Clinical Cancer Research Cervantes, E.V., Boucher, J.C., Lee, S.B., Spitler, K., Reid, K., Davila, M.L. (2019). MDSC Suppression of CAR T Cells Can be Reduced By Targeted Signaling Disruption. Blood 134(Supplement_1):4438. https://doi.org/10.1182/blood-2019- 122752
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