PRAVEENA DHULIPALA

Cell: 516-***-****

ade00u@r.postjobfree.com.

Committed to maintaining quality, compliance and efficiency

OBJECTIVE: Position as clinical/Senior Microbiologist or Research position that could utilize my diverse set of skills.

EXPERIENCE

Bio-Botanica, Inc., Hauppauge, N.Y. April 2017-December 2019

QC Microbiologist

Senior Microbiologist (QC & R&D)/Sanitation supervisor January 2020-Present

Estee Lauder, Melville, N.Y. January 2016-March 2017

QC Microbiologist

LIU Post College January 2016-August 2016

Microbiology Research Assistant under the direction of Marci J. Swede, Ph.D., Chair and Associate Professor, Department of Health Sciences

LIU Post College August 2014-May 2015

Chemistry Laboratory Teaching Assistant under the direction of Professor Neil Edwards, Laboratory Manager

Sankhya Diagnostic Services, India May 2012-June 2013

Clinical Laboratory Microbiologist

Mamata Medical College, Khammam, India May 2010-June 2011

Microbiology Clinical Laboratory Assistant and Teaching assistant under the direction of Suguna Hemachander, M.D., Professor and Head of the Department of Microbiology

EDUCATION

LONG ISLAND UNIVERSITY, NEW YORK August 2013-January 2016

MS in Medical Microbiology

Thesis Title: pbp1 protein localization under stress and non-stress conditions in xrr1 mutant.

Polymerase Chain Reaction amplification test (PCR) and DNA gel electrophoresis was utilized in this study.

Stress granules observed through fluorescent microscopy.

MAMATA MEDICAL COLLEGE, KHAMMAM, INDIA

MSc in Medical Microbiology.

Thesis Title: Bacteriological analysis of drinking water in relation to diarrheic illness in and around

Khammam

Techniques used in this study, included the following: Analysis of stool and water samples; Microscopic examination of stool for parasitic eggs; Water testing by MPN method and H2S Himedia Rapid kit; Shigella spp. identification by sero-agglutination; Salmonella spp. identification by ELISA

CLINICAL HOURS

Mamata Medical Hospital, Khammam, India

Microbiology Laboratory Assistant.

Sample collection, labeling and processing

ELISA for HIV and HBV / Western blot test for confirmation.

Prepared smears from bodily fluid samples, Gram stained and acid-fast stained such smears and further examined them by microscopy

Plated clinical specimens on appropriate microbiological media.

Aseptically worked in a BSL-2 & BSL-3 environment to cultivate Mycobacterium tuberculosis

Examination of stool samples for parasitic eggs by Formal Ether sedimentation technique.

Immunomagnetic separation method for water samples -parasitic cysts/oocysts.

Maintenance of ATCC Microorganisms for use as control strains

Culture media preparation

Antibiotic susceptibility testing by Kirby Bauer disk diffusion method

Clinical report writing

Environmental monitoring

Experience with handling pathogenic strains

Trained on cultivating viruses on eggs and subsequently spotted pocks for identification.

Animal handling, including rabbits, mice and Guinea pigs

Serological testing, including: Rapid method for Plasmodium spp.; Anti-Streptolysin-O

for S. pyogenes; C-reactive protein for inflammation; Rheumatoid factor test; HCG Pregnancy test.

JAGARLAMUDI KUPPUSWAMY CHOWDARY COLLEGE, GUNTUR, INDIA

BSC in Microbiology Minor: Biochemistry and Chemistry

Attended and participated in multiple seminars

Participation in a science exhibition, obtaining 1st prize for the creation of a mosaic virus model.

ACHIEVEMENTS

SUNNY, Old Westbury College, New York, 2016

Participated in MACUB conference: Developmental Biology and Genetics

Won 2nd place award in Graduate level poster presentation

“An interconnection between rapamycin resistance phenotype and stress granule markers in yeast”

Publication: “XRR1 is a novel gene that exhibits a conditional rapamycin resistant phenotype”

Suwal, R., Rivera, V., Dhulipala, P., Chowdhury, S., Swede, M.

Submitted (2017) to Cell Stress and Chaperons. Currently under revision

Thesis publication: Annals of Tropical Medicine and Public Health Journal. “Bacteriological analysis of drinking water in relation to diarrheic illness in and around Khammam”, Volume 6, No. 6, Nov-Dec 2013, pp. 618-621.

LABORATORY EXPERIENCE

SKILLS

Performance of Bioburden for Total Aerobic Plate counts, Combined Yeast Mold Counts and Tests for Objectionable Organisms with more than five years of experience

Endotoxin testing LAL-gel clot with 2 years of experience

Heterotrophic testing

Colony counter

Product release OOS and CAPA

Media preparation and control via Growth Promotion and pH testing

Equipment cleaning verification by ATP swabbing and specific allergen testing

Microorganism identification by API and Vitek

Biolumix

Practices superb aseptic technique

Tests for both Milk and Gluten allergens in products via ELISA method

Has worked extensively with not only ATCC control organisms, but also with a multitude of clinical pathogens

Grams staining, Acid -Fast staining, wet mount, Dye staining techniques. Specimens for Parasitic eggs and larvae

Molecular Method-PCR, Gel-Electrophoresis

Water analysis by Milliflex filtration apparatus

Environmental monitoring utilizing both, settling plates and the RCS; contact plates; and swabs for specific pathogens including Listeria spp. at production sites in industry-Project lead.

Environmental monitoring in clinical areas, medical devices after sterilization with Formaldehyde, ethylene oxide gases and dry or moist heat.

Performance of compressed air testing

Has worked within BSL-2 and BSL-3 environments

Excellent documentation skills

Knowledge of OSHA regulations

Knowledge of USP methods, BAM, AOAC, FDA, NSF regulations, cGMPs (21CFR211), and cGLPs

HACCP

Writing SOPs

Seven months of Management experience, mentor for new colleagues

Purchases Microbiology laboratory supplies in a cost-efficient manner.

Training the technicians and production with sanitation methods.

At present pursuing clinical Pathology online certification.

COMPUTER SKILLS

MS word / MS excel / Power point / LIMS / Batch Metric / Smart Sheets/Electronic notebook

Excellent communication skills.

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