Senior microbiologist
Resume:
PRAVEENA DHULIPALA
Cell: 516-***-****
*******************@*****.***.
Committed to maintaining quality, compliance and efficiency
OBJECTIVE: Position as clinical/Senior Microbiologist or Research position that could utilize my diverse set of skills.
EXPERIENCE
Bio-Botanica, Inc., Hauppauge, N.Y. April 2017-December 2019
QC Microbiologist
Senior Microbiologist (QC & R&D)/Sanitation supervisor January 2020-Present
Estee Lauder, Melville, N.Y. January 2016-March 2017
QC Microbiologist
LIU Post College January 2016-August 2016
Microbiology Research Assistant under the direction of Marci J. Swede, Ph.D., Chair and Associate Professor, Department of Health Sciences
LIU Post College August 2014-May 2015
Chemistry Laboratory Teaching Assistant under the direction of Professor Neil Edwards, Laboratory Manager
Sankhya Diagnostic Services, India May 2012-June 2013
Clinical Laboratory Microbiologist
Mamata Medical College, Khammam, India May 2010-June 2011
Microbiology Clinical Laboratory Assistant and Teaching assistant under the direction of Suguna Hemachander, M.D., Professor and Head of the Department of Microbiology
EDUCATION
LONG ISLAND UNIVERSITY, NEW YORK August 2013-January 2016
MS in Medical Microbiology
Thesis Title: pbp1 protein localization under stress and non-stress conditions in xrr1 mutant.
Polymerase Chain Reaction amplification test (PCR) and DNA gel electrophoresis was utilized in this study.
Stress granules observed through fluorescent microscopy.
MAMATA MEDICAL COLLEGE, KHAMMAM, INDIA
MSc in Medical Microbiology.
Thesis Title: Bacteriological analysis of drinking water in relation to diarrheic illness in and around
Khammam
Techniques used in this study, included the following: Analysis of stool and water samples; Microscopic examination of stool for parasitic eggs; Water testing by MPN method and H2S Himedia Rapid kit; Shigella spp. identification by sero-agglutination; Salmonella spp. identification by ELISA
CLINICAL HOURS
Mamata Medical Hospital, Khammam, India
Microbiology Laboratory Assistant.
Sample collection, labeling and processing
ELISA for HIV and HBV / Western blot test for confirmation.
Prepared smears from bodily fluid samples, Gram stained and acid-fast stained such smears and further examined them by microscopy
Plated clinical specimens on appropriate microbiological media.
Aseptically worked in a BSL-2 & BSL-3 environment to cultivate Mycobacterium tuberculosis
Examination of stool samples for parasitic eggs by Formal Ether sedimentation technique.
Immunomagnetic separation method for water samples -parasitic cysts/oocysts.
Maintenance of ATCC Microorganisms for use as control strains
Culture media preparation
Antibiotic susceptibility testing by Kirby Bauer disk diffusion method
Clinical report writing
Environmental monitoring
Experience with handling pathogenic strains
Trained on cultivating viruses on eggs and subsequently spotted pocks for identification.
Animal handling, including rabbits, mice and Guinea pigs
Serological testing, including: Rapid method for Plasmodium spp.; Anti-Streptolysin-O
for S. pyogenes; C-reactive protein for inflammation; Rheumatoid factor test; HCG Pregnancy test.
JAGARLAMUDI KUPPUSWAMY CHOWDARY COLLEGE, GUNTUR, INDIA
BSC in Microbiology Minor: Biochemistry and Chemistry
Attended and participated in multiple seminars
Participation in a science exhibition, obtaining 1st prize for the creation of a mosaic virus model.
ACHIEVEMENTS
SUNNY, Old Westbury College, New York, 2016
Participated in MACUB conference: Developmental Biology and Genetics
Won 2nd place award in Graduate level poster presentation
“An interconnection between rapamycin resistance phenotype and stress granule markers in yeast”
Publication: “XRR1 is a novel gene that exhibits a conditional rapamycin resistant phenotype”
Suwal, R., Rivera, V., Dhulipala, P., Chowdhury, S., Swede, M.
Submitted (2017) to Cell Stress and Chaperons. Currently under revision
Thesis publication: Annals of Tropical Medicine and Public Health Journal. “Bacteriological analysis of drinking water in relation to diarrheic illness in and around Khammam”, Volume 6, No. 6, Nov-Dec 2013, pp. 618-621.
LABORATORY EXPERIENCE
SKILLS
Performance of Bioburden for Total Aerobic Plate counts, Combined Yeast Mold Counts and Tests for Objectionable Organisms with more than five years of experience
Endotoxin testing LAL-gel clot with 2 years of experience
Heterotrophic testing
Colony counter
Product release OOS and CAPA
Media preparation and control via Growth Promotion and pH testing
Equipment cleaning verification by ATP swabbing and specific allergen testing
Microorganism identification by API and Vitek
Biolumix
Practices superb aseptic technique
Tests for both Milk and Gluten allergens in products via ELISA method
Has worked extensively with not only ATCC control organisms, but also with a multitude of clinical pathogens
Grams staining, Acid -Fast staining, wet mount, Dye staining techniques. Specimens for Parasitic eggs and larvae
Molecular Method-PCR, Gel-Electrophoresis
Water analysis by Milliflex filtration apparatus
Environmental monitoring utilizing both, settling plates and the RCS; contact plates; and swabs for specific pathogens including Listeria spp. at production sites in industry-Project lead.
Environmental monitoring in clinical areas, medical devices after sterilization with Formaldehyde, ethylene oxide gases and dry or moist heat.
Performance of compressed air testing
Has worked within BSL-2 and BSL-3 environments
Excellent documentation skills
Knowledge of OSHA regulations
Knowledge of USP methods, BAM, AOAC, FDA, NSF regulations, cGMPs (21CFR211), and cGLPs
HACCP
Writing SOPs
Seven months of Management experience, mentor for new colleagues
Purchases Microbiology laboratory supplies in a cost-efficient manner.
Training the technicians and production with sanitation methods.
At present pursuing clinical Pathology online certification.
COMPUTER SKILLS
MS word / MS excel / Power point / LIMS / Batch Metric / Smart Sheets/Electronic notebook
Excellent communication skills.
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