ANDREINA GOMEZ Email: addwu2@r.postjobfree.com

Phone: 305-***-****

MSc Microbiology 2710 Segovia Coral Gables, Florida 33134 PERSONAL STATEMENT: I am a motivated, driven, and bilingual Biologist, MSc in Microbiology and MSc in Bioethics, with strong knowledge and academic background in research and working in the industrial field. Always committed to professional ethics, proactive, interested in team work and acquiring new knowledge s and experiences with a high level of organizational skills, research development, team coordination and leadership.

EDUCATION AND TRAINING:

Master of Science in Bioethics

2018 – 2019 St. Thomas University GPA 3.949

Miami, FL USA

Master of Science in Microbiology

2013 – 2014 University of Granada GPA 3.810

Granada, Spain

Bachelor of Science in Biology

2005 – 2012 Simon Bolivar University

Caracas, Venezuela

WORK AND RESEARCH EXPERIENCE:

2016 – 2018: RESEARCHER ASSOCIATE at Simon Bolivar University. Immunology of Infectious Diseases Laboratory (Caracas, Venezuela).

- Inoculation of Plasmodium in murine models by intraperitoneal injection and development of parasitemia curves.

- Evaluation of new compounds for the use and application of antimalarial drugs in murine model.

- Performance of Indirect Enzyme-linked Immunosorbent assay (ELISA).

- Analysis and interpretation of ELISA data and elaboration of protocols for the study of Plasmodium on pregnant rodents BALB/c.

- Preparation and development of cell cultures and cell proliferation assays using Vero cell line.

- Performance of research reports.

- Design of protocols and procedures for specific projects.

- Performance of various editorial duties, working closely with research members assisting with proofreading for manuscripts as well as providing support in the preparation of lab protocols.

- Participation and development of published research studies.

- Training and coordination of activities for pre graduated students, peers and technical assistants. 2013 – 2015: RESEARCHER ASSISTANT (GRAD STUDENT) at University of Granada. Microbial Exopolysaccharides Laboratory (Granada, Spain).

- Performance of morphological, biochemical and enzymatic tests on gram-negative and gram-positive bacteria.

- Laboratory tests for the study of the effects of salt concentration on bacterial growth.

- Bacterial DNA extraction and quantification.

- Performance of amplification of the 16S rRNA gene with polymerase chain reaction (PCR).

- Sequencing data analysis through BioEdit and EzTaxon database for the characterization of new species.

- Construction of phylogenetic trees through MEGA (Molecular Evolutionary Genetics Analysis).

- Performance of Agarose Gel Electrophoresis for the analysis of PCR products.

- Development of new growth media formulas for the isolation and preservation of not-yet-cultured microorganisms.

- Extreme microorganisms study and analysis, followed by evaluation of their application in biotechnology.

- Multidisciplinary work with team members of the pharmaceutical industry in the development of microbiology experiments and isolation of microorganisms.

- Participation and development of research reports and technical papers for publishing.

- Bioburden of BSC.

- Sterilization of biological waste and infectious material.

- Setting up, operating and maintaining laboratory instruments and buffer preparation. 2012 – 2013: RESEARCHER ASSISTANT at Institute of Advanced Studies Foundation (IDEA). Biotechnology Lab (Caracas, Venezuela).

- Isolation, characterization, and identification of foodborne bacteria.

- Gram and spore staining.

- Isolation and biopreservation of pure cultures of fungi.

- Microscopic examination and conventional identification of fungi from environmental samples.

- In vitro propagation of plants and In vitro inoculation with Fungi and Bacteria in plants.

- Performance of In vitro antimicrobial assays of infected plants.

- Bioburden testing of water.

- Maintenance of lab safety and hazardous waste management.

- Organization and coordination of the XXIII Venezuelan Annual Congress of Phytopathology 2013.

- Management of inventory and order of laboratory supplies including glassware, and chemicals for biotech processes.

- Elaboration and supervision of safety laboratory protocols. Practice of biosafety and GLP. 2010 – 2011: INTERN at Polar Brewery. Laboratory of Microbiology (Caracas, Venezuela).

- Isolation and identification of foodborne microorganisms in critical control points of beer production including anaerobic spore-forming bacteria of the genus Bacillus and Clostridium and pathogens. Performance of biochemical testing for bacteria identification.

- Development and maintenance of a culture collection for microorganisms isolated in the brewery production process.

- Microbial testing of water samples collected from critical points of beer and beverage production process using membrane filtration vacuums.

- Performance, analysis, and interpretation of microbiological testing of finished products. Environmental monitoring of in-process and finished product beverages (Gram Staining, Growth Promotion Test (GPT), Total microbial aerobic count).

- Bioburden of Biological safety cabinets.

- Data review of microbiologic test results and screening of detection for specified microorganisms.

- Design and development of standard protocols to assess the reliability of microbiological tests for the implementation of ISO 17025 management system.

- Review of logbooks associated with product testing results and lab equipment calibration.

- Research of documentation of safety standards in the food industry.

- Preparation and storage of microbiological culture media.

- Sterilization and preparation of lab instruments (pipette tips, Pyrex glassware, test tubes, inoculation loops, glass Petri dishes) growth culture media, and solutions.

- Steam sterilization of biological waste or infectious materials (bacteria cultures, contaminated glassware).

- Practice of biosafety, GMP and quality control procedures. 2009 – 2013: RESEARCHER ASSISTANT at Simon Bolivar University. Immunology of Infectious Diseases Laboratory (Caracas, Venezuela).

- Preparation of Hyper Immune Sera in murine model BALB/c.

- Performance of Indirect Enzyme-linked Immunosorbent assay (ELISA).

- Elaboration of SDS – PAGE gels, Immunoblotting and dot-ELISA.

- Isolation and characterization of antigens and antibodies by ELISA test using 96-well microplates and multichannel pipettes.

- Evaluation of hypersensitivity and humoral responses of new drugs using footpad swelling test in BALB/c mice.

- Analysis and interpretation of experimental data.

- Identification of microorganisms of the genus Staphylococcus and Salmonella.

- Performance of Biofilm formation assay. - Laboratory supplies and equipment management. 2008: TECHNICAL ASSISTANT at Simon Bolivar University. Microbiology and Mycology Laboratory

(Caracas, Venezuela).

- Culture media preparation.

- Laboratory instrument sterilization and preparation.

- Preparation of solutions and buffers.

- Isolation, recovery, and preservation of fungi cultures using selective media.

- Soil sample processing for future microbiology tests.

- Mycology and Microbiology Laboratory organization.

- Sterilization of biological waste and infectious material. SKILLS:

Communication Skills and Organizational Skills

- Strong written and verbal communication skills in Spanish and English, gained through my experience as a Researcher.

- Project management skills and strong scientific and research background.

- Excellent cultural interaction gained from my experience living in different countries.

- Ability to maintain company confidentiality.

- High work ethics and a positive attitude towards all tasks.

- Strong willingness to learn and adapt while seeking ways for continuous improvement.

- Excellent time management and organization skills.

- Ability to work on multiple tasks simultaneously and prioritize where necessary.

- Self- motivated, results-driven and dedicated to performing all tasks to the best of my ability. Job-related Skills

- Ability to handle several microbiologic samples in a short period of time.

- Excellent skills to work in sterile conditions.

- High capability to solve problems using scientific rules and methods.

- Ability to work with animals with bioethics knowledge.

- Proficient and qualified in the use and management of specialized equipment such as autoclave, microscopes, electrophoresis units, laminar flow cabinets, electrophoresis units, micro volume spectrophotometers, freeze dryers, thermo cyclers, membrane filtration vacuums and others.

- Understanding of Good Laboratory Practices and Good Manufacturing Practices. PUBLICATIONS:

● David J. Castro; Andreina Gomez-Altuve; José Carlos Reina; Miguel Rodríguez; Inmaculada Sampedro; Inmaculada Llamas; Fernando Martínez-Checa. Roseovarius bejariae sp. nov. - a moderately Halophilic bacterium isolated from a hyper saline steep-sided river bed in Murcia, south-eastern Spain. International Journal of Systematic and Evolutionary Microbiology. Accepted on March 2020, manuscript in production.

● Lilian M. Spencer, Andreina Gomez and Eva Collovini. Mechanisms of invasion from sporozoite and merozoite of Plasmodium. Bionatura 2016; Vol. 1:2.

● Lilian Spencer, Anaely Agola, Eduardo Roa, Neira Gamboa, Andreina Gomez and Jaime Charris. Humoral response against hemozoin from Plasmodium berghei and synthetic hemozoin in experimental model of BALB/c mice. British Journal of Medicine and Medical Research 2014; Vol. 4:20.

● Lilian Spencer, Valentina Pedrotti, Deisy Perdomo, Andreina Gomez, Armando Díaz and Cristina Castelli. Characterization of soluble antigens of Staphylococcus spp isolated from dairy farms in Venezuela. Microbiology Research 2012; Vol. 3:e25.

PRESENTATIONS:

● David J. Castro; Andreina Gomez-Altuve; José Carlos Reina; Miguel Rodríguez; Inmaculada Sampedro; Inmaculada Llamas; Fernando Martínez-Checa. “Roseovarius bejariae sp. a bacteria isolated from Rambla Salada (Murcia) using dilution by extinction method”. XII National Congress of Microbiology 2019. Malaga, Spain.

• Andreina Gomez, David Castro, Emilia Quesada, Victoria Béjar and Fernando Martínez-Checa. “Phenotypic evaluation and taxonomic approach of halophilic microorganisms isolated from Rambla Salada (Murcia), using dilution by extinction method”. XII Meeting of the National Network of Extremophiles Microorganisms

(REDEX) 2014. Madrid, Spain.

• Lilian Spencer, Andreina Gomez, Nieves Canudas and Emiliana Mendoza. “Study of the hypersensitivity of photosensitizing compounds”. XXIV IUPAC Symposium on Photochemistry 2012. Coimbra, Portugal.

• Lilian Spencer, Emiliana Mendoza, Nieves Canudas and Andreina Gomez. “Evaluation of the humoral response to photosensitizing compounds in their ground state”. XXIV IUPAC Symposium on Photochemistry 2012. Coimbra, Portugal.

CERTIFICATES, COURSES AND SEMINARS:

2020: Whole genome sequencing of bacterial genomes – tools and applications. Technical University of Denmark – Coursera.

2018: The Extremes of Life: Microbes and their Diversity. Kayoto University – edX. 2018: Astrobiology and the Search for Extraterrestrial Life. The University of Edinburgh – Coursera. 2017 – 2018: ESL (English as a second language) Asa College - Florida, United States. 2017: Microbiology Special Topics in Infectious Diseases. Libertador Experimental University (Venezuela). 2015: General Professional Proficiency, Plus (ILR3+) English. Inlingua International Language School (Florida, United States).

2013: Morphological Characterization and Molecular Biochemistry of Microorganisms. Conference forum course. Venezuelan Society of Plant Pathology and Institute of Advanced Studies Foundation (IDEA)

(Caracas, Venezuela).

2013: Diagnosis and Control of Phytopathogenic Microorganisms. Conference forum course. Venezuelan Society of Plant Pathology and Institute of Advanced Studies Foundation (IDEA) (Barinas, Venezuela). 2012: XXIV IUPAC Symposium of Photochemistry (Coimbra, Portugal). 2010: Update Seminar of Balanced Animal Food Production. Novus International, Inc. (Aragua, Venezuela). 2009: Microbiology Biosafety Seminar. Venezuelan Society of Microbiology (Caracas, Venezuela). REFERENCES:

- Dr. Fernando Martínez-Checa (Microbiology Department Director and Professor) University of Granada (Spain) addwu2@r.postjobfree.com

- Dr. Lilian Spencer (Professor)

University of Technology and Experimental Research (Ecuador) addwu2@r.postjobfree.com Simon Bolivar University (Venezuela) – Retired

- Jeannete Romero (Viral Media Manager, Bulk Manufacturing) Sanofi Pasteur (Canada) addwu2@r.postjobfree.com

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